scholarly journals Holographic optogenetic stimulation of patterned neuronal activity for vision restoration

2013 ◽  
Vol 4 (1) ◽  
Author(s):  
Inna Reutsky-Gefen ◽  
Lior Golan ◽  
Nairouz Farah ◽  
Adi Schejter ◽  
Limor Tsur ◽  
...  
Keyword(s):  
Neuronal Activity ◽  
Optogenetic Stimulation ◽  
Vision Restoration ◽  
Stimulation Of

scholarly journals Modification of oxygen consumption and blood flow in mouse somatosensory cortex by cell-type-specific neuronal activity

2019 ◽  
Author(s):  
Matilda Dahlqvist ◽  
Kirsten Thomsen ◽  
Dmitry Postnov ◽  
Martin Lauritzen
Keyword(s):  
Neuronal Activity ◽  
Pyramidal Neurons ◽  
Rhythmic Activity ◽  
Inhibitory Neurons ◽  
Gamma Activity ◽  
Somatosensory Stimulation ◽  
Neuronal Excitation ◽  
Optogenetic Stimulation ◽  
Higher Cognitive Functions ◽  
Stimulation Of

AbstractGamma activity arises from the interplay between pyramidal neurons and fast-spiking parvalbumin (PV) interneurons, is an integral part of higher cognitive functions and is assumed to contribute importantly to brain metabolic responses. Cerebral metabolic rate of oxygen (CMRO2) responses were evoked by optogenetic stimulation of cortical PV interneurons and pyramidal neurons. We found that CMRO2 responses depended on neuronal activation, but not on the power of gamma activity induced by optogenetic stimulation. This implies that evoked gamma activity per se is not energy demanding. Optogenetic stimulation of PV interneurons during somatosensory stimulation reduced excitatory neuronal activity but did not potentiate O2 consumption as previously hypothesized. In conclusion, our data suggest that activity-driven CMRO2 responses depend on neuronal excitation rather than the cerebral rhythmic activity they induce. Excitation of both excitatory and inhibitory neurons requires energy, but inhibition of cortical excitatory neurons by interneurons does not potentiate activity-driven energy consumption.

scholarly journals Modification of oxygen consumption and blood flow in mouse somatosensory cortex by cell-type-specific neuronal activity

2019 ◽  
Vol 40 (10) ◽  
pp. 2010-2025 ◽  
Author(s):  
Matilda K Dahlqvist ◽  
Kirsten J Thomsen ◽  
Dmitry D Postnov ◽  
Martin J Lauritzen
Keyword(s):  
Neuronal Activity ◽  
Pyramidal Neurons ◽  
Rhythmic Activity ◽  
Inhibitory Neurons ◽  
Gamma Activity ◽  
Somatosensory Stimulation ◽  
Neuronal Excitation ◽  
Optogenetic Stimulation ◽  
Higher Cognitive Functions ◽  
Stimulation Of

Gamma activity arising from the interplay between pyramidal neurons and fast-spiking parvalbumin (PV) interneurons is an integral part of higher cognitive functions and is assumed to contribute significantly to brain metabolic responses. Cerebral metabolic rate of oxygen (CMRO2) responses were evoked by optogenetic stimulation of cortical PV interneurons and pyramidal neurons. We found that CMRO2 responses depended on neuronal activation, but not on the power of gamma activity induced by optogenetic stimulation. This implies that evoked gamma activity per se is not energy demanding. Optogenetic stimulation of PV interneurons during somatosensory stimulation reduced excitatory neuronal activity but did not potentiate O2 consumption as previously hypothesized. In conclusion, our data suggest that activity-driven CMRO2 responses depend on neuronal excitation rather than the cerebral rhythmic activity they induce. Excitation of both excitatory and inhibitory neurons requires energy, but inhibition of cortical excitatory neurons by interneurons does not potentiate activity-driven energy consumption.

scholarly journals Optogenetic stimulation of infralimbic PFC reproduces ketamine’s rapid and sustained antidepressant actions

2015 ◽  
Vol 112 (26) ◽  
pp. 8106-8111 ◽  
Author(s):  
Manabu Fuchikami ◽  
Alexandra Thomas ◽  
Rongjian Liu ◽  
Eric S. Wohleb ◽  
Benjamin B. Land ◽  
...  
Keyword(s):  
Prefrontal Cortex ◽  
Neuronal Activity ◽  
Pyramidal Neurons ◽  
Rodent Models ◽  
Cellular Mechanisms ◽  
Optogenetic Stimulation ◽  
Layer V ◽  
And Function ◽  
Stimulation Of ◽  
Synaptic Responses

Ketamine produces rapid and sustained antidepressant actions in depressed patients, but the precise cellular mechanisms underlying these effects have not been identified. Here we determined if modulation of neuronal activity in the infralimbic prefrontal cortex (IL-PFC) underlies the antidepressant and anxiolytic actions of ketamine. We found that neuronal inactivation of the IL-PFC completely blocked the antidepressant and anxiolytic effects of systemic ketamine in rodent models and that ketamine microinfusion into IL-PFC reproduced these behavioral actions of systemic ketamine. We also found that optogenetic stimulation of the IL-PFC produced rapid and long-lasting antidepressant and anxiolytic effects and that these effects are associated with increased number and function of spine synapses of layer V pyramidal neurons. The results demonstrate that ketamine infusions or optogenetic stimulation of IL-PFC are sufficient to produce long-lasting antidepressant behavioral and synaptic responses similar to the effects of systemic ketamine administration.

scholarly journals Optogenetic stimulation of glutamatergic neuronal activity in the striatum enhances neurogenesis in the subventricular zone of normal and stroke mice

2017 ◽  
Vol 98 ◽  
pp. 9-24 ◽  
Author(s):  
Mingke Song ◽  
Shan Ping Yu ◽  
Osama Mohamad ◽  
Wenyuan Cao ◽  
Zheng Zachory Wei ◽  
...  
Keyword(s):  
Neuronal Activity ◽  
Subventricular Zone ◽  
Optogenetic Stimulation ◽  
Stimulation Of

scholarly journals Incerto-thalamic modulation of fear via GABA and dopamine

2021 ◽  
Author(s):  
Archana Venkataraman ◽  
Sarah C. Hunter ◽  
Maria Dhinojwala ◽  
Diana Ghebrezadik ◽  
JiDong Guo ◽  
...  
Keyword(s):  
Brain Regions ◽  
Zona Incerta ◽  
Dependent Manner ◽  
Post Traumatic Stress ◽  
Functional Roles ◽  
Functional Connections ◽  
Optogenetic Stimulation ◽  
Fear Generalization ◽  
Stimulation Of

AbstractFear generalization and deficits in extinction learning are debilitating dimensions of Post-Traumatic Stress Disorder (PTSD). Most understanding of the neurobiology underlying these dimensions comes from studies of cortical and limbic brain regions. While thalamic and subthalamic regions have been implicated in modulating fear, the potential for incerto-thalamic pathways to suppress fear generalization and rescue deficits in extinction recall remains unexplored. We first used patch-clamp electrophysiology to examine functional connections between the subthalamic zona incerta and thalamic reuniens (RE). Optogenetic stimulation of GABAergic ZI → RE cell terminals in vitro induced inhibitory post-synaptic currents (IPSCs) in the RE. We then combined high-intensity discriminative auditory fear conditioning with cell-type-specific and projection-specific optogenetics in mice to assess functional roles of GABAergic ZI → RE cell projections in modulating fear generalization and extinction recall. In addition, we used a similar approach to test the possibility of fear generalization and extinction recall being modulated by a smaller subset of GABAergic ZI → RE cells, the A13 dopaminergic cell population. Optogenetic stimulation of GABAergic ZI → RE cell terminals attenuated fear generalization and enhanced extinction recall. In contrast, optogenetic stimulation of dopaminergic ZI → RE cell terminals had no effect on fear generalization but enhanced extinction recall in a dopamine receptor D1-dependent manner. Our findings shed new light on the neuroanatomy and neurochemistry of ZI-located cells that contribute to adaptive fear by increasing the precision and extinction of learned associations. In so doing, these data reveal novel neuroanatomical substrates that could be therapeutically targeted for treatment of PTSD.

scholarly journals Involvement of MCH-oxytocin neural relay within the hypothalamus in murine nursing behavior

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yoko Kato ◽  
Harumi Katsumata ◽  
Ayumu Inutsuka ◽  
Akihiro Yamanaka ◽  
Tatsushi Onaka ◽  
...  
Keyword(s):  
Mammalian Species ◽  
Virgin Female ◽  
Essential Elements ◽  
Hypothalamic Nucleus ◽  
Male Mice ◽  
Hypothalamic Area ◽  
Functional Roles ◽  
Genetic Ablation ◽  
Optogenetic Stimulation ◽  
Stimulation Of

AbstractMultiple sequential actions, performed during parental behaviors, are essential elements of reproduction in mammalian species. We showed that neurons expressing melanin concentrating hormone (MCH) in the lateral hypothalamic area (LHA) are more active in rodents of both sexes when exhibiting parental nursing behavior. Genetic ablation of the LHA-MCH neurons impaired maternal nursing. The post-birth survival rate was lower in pups born to female mice with congenitally ablated MCH neurons under control of tet-off system, exhibiting reduced crouching behavior. Virgin female and male mice with ablated MCH neurons were less interested in pups and maternal care. Chemogenetic and optogenetic stimulation of LHA-MCH neurons induced parental nursing in virgin female and male mice. LHA-MCH GABAergic neurons project fibres to the paraventricular hypothalamic nucleus (PVN) neurons. Optogenetic stimulation of PVN induces nursing crouching behavior along with increasing plasma oxytocin levels. The hypothalamic MCH neural relays play important functional roles in parental nursing behavior in female and male mice.

Sign in / Sign up

Export Citation Format

Share Document