scholarly journals A cost-effective method to immobilize hydrated soft-tissue samples for atomic force microscopy

BioTechniques ◽  
2016 ◽  
Vol 61 (4) ◽  
Author(s):  
Suchit Sahai ◽  
Marysuna Wilkerson ◽  
Ana Maria Zaske ◽  
Scott D. Olson ◽  
Charles S. Cox ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Soft Tissue ◽  
Cost Effective ◽  
Tissue Samples ◽  
Force Microscopy ◽  
Atomic Force ◽  
Cost Effective Method

scholarly journals Antibacterial Activity of Silver Nanoparticles Synthesized by Bark Extract of Syzygium cumini

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Ram Prasad ◽  
Vyshnava Satyanarayana Swamy
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Cost Effective ◽  
Diffusion Method ◽  
Bark Extract ◽  
Force Microscopy ◽  
Atomic Force ◽  
Cost Effective Method ◽  
Uv Visible ◽  
Major Attention

The unique property of the silver nanoparticles having the antimicrobial activity drags the major attention towards the present nanotechnology. The environmentally nontoxic, ecofriendly, and cost-effective method that has been developed for the synthesis of silver nanoparticles using plant extracts creates the major research interest in the field of nanobiotechnology. The synthesized silver nanoparticles have been characterized by the UV-visible spectroscopy, atomic force microscopy (AFM), and scanning electron microscopy (SEM). Further, the antibacterial activity of silver nanoparticles was evaluated by well diffusion method, and it was found that the biogenic silver nanoparticles have antibacterial activity against Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 29213), Pseudomonas aeruginosa (ATCC 27853), Azotobacter chroococcum WR 9, and Bacillus licheniformis (MTCC 9555).

Peel-off probe: a cost-effective probe for electrical atomic force microscopy

2000 ◽  
Author(s):  
Thomas Hantschel ◽  
Stefan Slesazeck ◽  
N. Duhayon ◽  
Mingwei Xu ◽  
Wilfried Vandervorst
Keyword(s):  
Atomic Force Microscopy ◽  
Cost Effective ◽  
Force Microscopy ◽  
Atomic Force

Determination of mechanical properties of soft tissue scaffolds by atomic force microscopy nanoindentation

2011 ◽  
Vol 44 (13) ◽  
pp. 2356-2361 ◽  
Author(s):  
Yanxia Zhu ◽  
Zhuxin Dong ◽  
Uchechukwu C. Wejinya ◽  
Sha Jin ◽  
Kaiming Ye
Keyword(s):  
Mechanical Properties ◽  
Atomic Force Microscopy ◽  
Soft Tissue ◽  
Tissue Scaffolds ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals DNA Nanobiosensors: An Outlook on Signal Readout Strategies

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Arun Richard Chandrasekaran
Keyword(s):  
Atomic Force Microscopy ◽  
Gel Electrophoresis ◽  
Point Of Care ◽  
Cost Effective ◽  
Diagnostic Tools ◽  
Force Microscopy ◽  
Atomic Force ◽  
Structural Versatility ◽  
Signal Readout

A suite of functionalities and structural versatility makes DNA an apt material for biosensing applications. DNA-based biosensors are cost-effective and sensitive and have the potential to be used as point-of-care diagnostic tools. Along with robustness and biocompatibility, these sensors also provide multiple readout strategies. Depending on the functionality of DNA-based biosensors, a variety of output strategies have been reported: fluorescence- and FRET-based readout, nanoparticle-based colorimetry, spectroscopy-based techniques, electrochemical signaling, gel electrophoresis, and atomic force microscopy.

scholarly journals Comparative effect of three polishing systems on porcelain surface roughness after orthodontic bracket debonding and composite resin removal: An atomic force microscopy

2019 ◽  
Vol 9 ◽  
pp. 223-229
Author(s):  
Faeze Qabel ◽  
Riehane Talaei ◽  
Saeedeh Saeedi ◽  
Raheb Ghorbani ◽  
Nazila Ameli
Keyword(s):  
Surface Roughness ◽  
Atomic Force Microscopy ◽  
Composite Resin ◽  
Cost Effective ◽  
Orthodontic Bracket ◽  
Force Microscopy ◽  
Atomic Force ◽  
The Mean ◽  
Level Of Significance ◽  
One Way Anova

Purpose: Porcelain polishing after orthodontic bracket debonding and resin removal is imperative to eliminate surface roughness and minimize the risk of plaque accumulation, periodontal disease, and porcelain discoloration. This study aimed to assess the effect of three polishing systems on porcelain surface roughness after orthodontic bracket debonding. Materials and Methods: Thirty porcelain blocks were divided into three groups. Surface roughness of the samples was first measured using atomic force microscopy (AFM) and recorded as baseline. Orthodontic brackets were bonded to blocks by composite resin. After bracket debonding, resin remnants were removed by tungsten carbide bur. The blocks were then polished with Sof-Lex discs, Meisinger, and Jota porcelain polishing kit. Surface roughness was measured again using AFM. The Shapiro–Wilk test, one-way ANOVA, and Tukey’s post hoc test were used for data analysis through SPSS version 18.0. Level of significance was set at 5%. Results: The mean change in surface roughness after polishing with Jota kit (56.6 nm) was significantly greater than that compared to Sof-Lex discs (10.7 nm) (P = 0.003) and Meisinger kit (26.6 nm) (P = 0.024). The mean change in surface roughness was not significantly different between Sof-Lex and Meisinger groups. Surface roughness significantly increased in all three groups (P < 0.05). Conclusion: Meisinger polishing kit and Sof-Lex discs were not significantly different in terms of the resultant surface roughness. Thus, the conventional use of Sof-Lex discs seems to be more cost-effective due to their lower cost.

scholarly journals Test-retest reproducibility of atomic force microscopy measurements of human trabecular meshwork stiffness

2020 ◽  
Vol 2 (4) ◽  
pp. 34-43
Author(s):  
Larry Kagemann ◽  
Joe Candiello ◽  
Gadi Wollstein ◽  
Hiroshi Ishikawa ◽  
Richard A. Bilonick ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Trabecular Meshwork ◽  
Human Donor ◽  
Tissue Samples ◽  
Force Microscopy ◽  
Young’S Moduli ◽  
Atomic Force ◽  
Coefficients Of Variation ◽  
Cryostat Sections ◽  
Anterior Posterior

Purpose: The purpose of the present study was to quantify test-retest reproducibility of measurements of stiffness of the human trabecular meshwork (HTM) by atomic force microscopy (AFM). Methods: Eleven 40 μm radial limbal cryostat sections from a fresh human donor rim were mounted on charged slides and rehydrated at room temperature. Stiffness at four TM locations (anterior to posterior along Schlemm’s canal) was measured by AFM. At each location, a 6 x 6 grid was sampled. Indentation points were evenly distributed over a 20 μm x 20 μm area, with a rate of one load/unload cycle per second. Measurements were then repeated for calculation of test-retest variability. Results: The test-retest coefficients of variation for the four measurement locations (anterior to posterior) were 24.39, 25.28, 12.74, and 14.26%, respectively, with a notable drop in the two posterior locations compared to the anterior. The test-retest coefficient for the sections was 19.17%. For the entire eye, the test-retest coefficient of variation for the measurement of the TM stiffness was 17.13%. Young’s moduli consistently decreased from anterior to posterior location. Conclusions: Wide regional variation suggests that single value does little to fully describe the complex array of TM stiffness levels within the eye, and future studies of TM stiffness assessed by AFM should include multiple tissue samples from each eye, with documentation of the anterior-posterior location of each measurement.

scholarly journals Detecting Protein Aggregates on Untreated Human Tissue Samples by Atomic Force Microscopy Recognition Imaging

2010 ◽  
Vol 99 (5) ◽  
pp. 1660-1667 ◽  
Author(s):  
Rhiannon Creasey ◽  
Shiwani Sharma ◽  
Jamie E. Craig ◽  
Christopher T. Gibson ◽  
Andreas Ebner ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Human Tissue ◽  
Protein Aggregates ◽  
Tissue Samples ◽  
Force Microscopy ◽  
Atomic Force ◽  
Recognition Imaging

scholarly journals Robust and artifact-free mounting of tissue samples for atomic force microscopy

BioTechniques ◽  
2014 ◽  
Vol 56 (1) ◽  
Author(s):  
Joshua T. Morgan ◽  
Vijay Krishna Raghunathan ◽  
Sara M. Thomasy ◽  
Christopher J. Murphy ◽  
Paul Russell
Keyword(s):  
Atomic Force Microscopy ◽  
Tissue Samples ◽  
Force Microscopy ◽  
Atomic Force

Development of a Novel Process Chain Based on Atomic Force Microscopy Scratching for Small and Medium Series Production of Polymer Nanostructured Components

2010 ◽  
Vol 132 (3) ◽  
Author(s):  
E. B. Brousseau ◽  
F. Krohs ◽  
E. Caillaud ◽  
S. Dimov ◽  
O. Gibaru ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Low Cost ◽  
Cost Effective ◽  
Direct Write ◽  
Series Production ◽  
Process Chain ◽  
Force Microscopy ◽  
Atomic Force ◽  
Patterned Structures ◽  
Functional Features

The continuing trend for producing novel micro- and nanostructured devices and components in a broad range of materials is a major motivating factor driving the research in the micro- and nanomanufacturing sector toward developing innovative process chains. Some of such chains enable the serial production of micro- and nanostructured parts in polymer material by combining innovatively and optimizing simultaneously master making and replication technologies. For producing features at the nanoscale, the master making processes that are currently commonly employed rely on complex lithography-based pattern transfers and/or on beam-based direct write processes. Unfortunately, the required equipment to perform these techniques are often capital intensive and necessitate particular operating temperatures or vacuum conditions. At the same time, during the development phase of new or improved nanotechnology-enabled products, it is beneficial to produce rapidly polymer prototypes to test the functionality of components with nanoscale features. Thus, the technologies currently available for nanostructuring replication masters do not comply with the low cost requirements typically associated with the production of small batches of components for prototyping purposes. As a result, this could restrict the successful development of products with functional features at the nanoscale. In this research, a new process chain is presented for the fabrication of nanostructured components in polymer that relies on a simple and cost-effective master making technology. In particular, atomic force microscopy scratching is employed as an alternative technique for nanostructuring replication masters for microinjection molding. The conducted experimental study demonstrated the potential of this approach for small and medium series production of nanostructured devices in thermoplastic materials. In addition, the effects of different scratching parameters on the achievable surface roughness and depth of the patterned structures were analyzed by employing the design of experiments approach.

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