scholarly journals DNA Nanobiosensors: An Outlook on Signal Readout Strategies

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Arun Richard Chandrasekaran
Keyword(s):  
Atomic Force Microscopy ◽  
Gel Electrophoresis ◽  
Point Of Care ◽  
Cost Effective ◽  
Diagnostic Tools ◽  
Force Microscopy ◽  
Atomic Force ◽  
Structural Versatility ◽  
Signal Readout

A suite of functionalities and structural versatility makes DNA an apt material for biosensing applications. DNA-based biosensors are cost-effective and sensitive and have the potential to be used as point-of-care diagnostic tools. Along with robustness and biocompatibility, these sensors also provide multiple readout strategies. Depending on the functionality of DNA-based biosensors, a variety of output strategies have been reported: fluorescence- and FRET-based readout, nanoparticle-based colorimetry, spectroscopy-based techniques, electrochemical signaling, gel electrophoresis, and atomic force microscopy.

Peel-off probe: a cost-effective probe for electrical atomic force microscopy

2000 ◽  
Author(s):  
Thomas Hantschel ◽  
Stefan Slesazeck ◽  
N. Duhayon ◽  
Mingwei Xu ◽  
Wilfried Vandervorst
Keyword(s):  
Atomic Force Microscopy ◽  
Cost Effective ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals High-sensitivity Detection of Proteins using Gel Electrophoresis and Atomic Force Microscopy

Hyomen Kagaku ◽  
2009 ◽  
Vol 30 (6) ◽  
pp. 351-356
Author(s):  
Hiroshi SEKIGUCHI ◽  
Atsushi HIDAKA ◽  
Yoshihito SHIGA ◽  
Atsushi IKAI ◽  
Toshiya OSADA
Keyword(s):  
Atomic Force Microscopy ◽  
Gel Electrophoresis ◽  
High Sensitivity ◽  
Force Microscopy ◽  
Atomic Force ◽  
High Sensitivity Detection ◽  
Sensitivity Detection

DNA interaction of ruthenium(ii) complexes with imidazo[4,5-f][1,10]phenanthroline derivatives

2019 ◽  
Vol 48 (12) ◽  
pp. 3914-3921 ◽  
Author(s):  
Bole Yu ◽  
Thomas W. Rees ◽  
Jiewen Liang ◽  
Chengzhi Jin ◽  
Yu Chen ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Atomic Force Microscopy ◽  
Light Scattering ◽  
Gel Electrophoresis ◽  
Dna Interaction ◽  
Force Microscopy ◽  
Atomic Force ◽  
Transmission Electron ◽  
Spectral Titration

The DNA interaction properties of four Ru(ii) complexes with imidazo[4,5-f][1,10]phenanthroline derivatives were investigated by spectral titration, gel electrophoresis (GAR), dynamic light scattering (DLS), zeta potential, atomic force microscopy (AFM), and transmission electron microscopy (TEM).

scholarly journals Separating DNA with Different Topologies by Atomic Force Microscopy in Comparison with Gel Electrophoresis

2010 ◽  
Vol 114 (37) ◽  
pp. 12162-12165 ◽  
Author(s):  
Yong Jiang ◽  
Mahir Rabbi ◽  
Piotr A. Mieczkowski ◽  
Piotr E. Marszalek
Keyword(s):  
Atomic Force Microscopy ◽  
Gel Electrophoresis ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Comparative effect of three polishing systems on porcelain surface roughness after orthodontic bracket debonding and composite resin removal: An atomic force microscopy

2019 ◽  
Vol 9 ◽  
pp. 223-229
Author(s):  
Faeze Qabel ◽  
Riehane Talaei ◽  
Saeedeh Saeedi ◽  
Raheb Ghorbani ◽  
Nazila Ameli
Keyword(s):  
Surface Roughness ◽  
Atomic Force Microscopy ◽  
Composite Resin ◽  
Cost Effective ◽  
Orthodontic Bracket ◽  
Force Microscopy ◽  
Atomic Force ◽  
The Mean ◽  
Level Of Significance ◽  
One Way Anova

Purpose: Porcelain polishing after orthodontic bracket debonding and resin removal is imperative to eliminate surface roughness and minimize the risk of plaque accumulation, periodontal disease, and porcelain discoloration. This study aimed to assess the effect of three polishing systems on porcelain surface roughness after orthodontic bracket debonding. Materials and Methods: Thirty porcelain blocks were divided into three groups. Surface roughness of the samples was first measured using atomic force microscopy (AFM) and recorded as baseline. Orthodontic brackets were bonded to blocks by composite resin. After bracket debonding, resin remnants were removed by tungsten carbide bur. The blocks were then polished with Sof-Lex discs, Meisinger, and Jota porcelain polishing kit. Surface roughness was measured again using AFM. The Shapiro–Wilk test, one-way ANOVA, and Tukey’s post hoc test were used for data analysis through SPSS version 18.0. Level of significance was set at 5%. Results: The mean change in surface roughness after polishing with Jota kit (56.6 nm) was significantly greater than that compared to Sof-Lex discs (10.7 nm) (P = 0.003) and Meisinger kit (26.6 nm) (P = 0.024). The mean change in surface roughness was not significantly different between Sof-Lex and Meisinger groups. Surface roughness significantly increased in all three groups (P < 0.05). Conclusion: Meisinger polishing kit and Sof-Lex discs were not significantly different in terms of the resultant surface roughness. Thus, the conventional use of Sof-Lex discs seems to be more cost-effective due to their lower cost.

Collagen Structure Deterioration in the Skin of Patients with Pelvic Organ Prolapse Determined by Atomic Force Microscopy

2015 ◽  
Vol 21 (2) ◽  
pp. 324-333 ◽  
Author(s):  
Svetlana L. Kotova ◽  
Peter S. Timashev ◽  
Anna E. Guller ◽  
Anatoly B. Shekhter ◽  
Pavel I. Misurkin ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Pelvic Organ Prolapse ◽  
Connective Tissue ◽  
Histological Study ◽  
Pelvic Organ ◽  
Diagnostic Tools ◽  
Pathological Changes ◽  
Significant Deterioration ◽  
Force Microscopy ◽  
Atomic Force

AbstractWe used atomic force microscopy (AFM) to diagnose pathological changes in the extracellular matrix (ECM) of skin connective tissue in patients with pelvic organ prolapse (POP). POP is a common condition affecting women that considerably decreases the patients’ quality of life. Deviations from normal morphology of the skin ECM from patients with POP occur including packing and arrangement of individual collagen fibers and arrangement of collagen fibrils. The nanoindentation study revealed significant deterioration of the mechanical properties of collagen fibril bundles in the skin of POP patients as compared with the skin of healthy subjects. Changes in the skin ECM appeared to correlate well with changes in the ECM of the pelvic ligament tissue associated with POP. AFM data on the ECM structure of normal and pathologically altered connective tissue were in agreement with results of the standard histological study on the same clinical specimens. Thus, AFM and related techniques may serve as independent or complementary diagnostic tools for tracking POP-related pathological changes of connective tissue.

High-sensitivity detection of proteins using gel electrophoresis and atomic force microscopy

2009 ◽  
Vol 109 (8) ◽  
pp. 916-922 ◽  
Author(s):  
Hiroshi Sekiguchi ◽  
Atsushi Hidaka ◽  
Yoshihito Shiga ◽  
Atsushi Ikai ◽  
Toshiya Osada
Keyword(s):  
Atomic Force Microscopy ◽  
Gel Electrophoresis ◽  
High Sensitivity ◽  
Force Microscopy ◽  
Atomic Force ◽  
High Sensitivity Detection ◽  
Sensitivity Detection

[Ru(TAP)3]2+-Photosensitized DNA Cleavage Studied by Atomic Force Microscopy and Gel Electrophoresis: A Comparative Study

2006 ◽  
Vol 12 (3) ◽  
pp. 758-762 ◽  
Author(s):  
Hiroshi Uji-i ◽  
Philippe Foubert ◽  
Frans C. De Schryver ◽  
Steven De Feyter ◽  
Etienne Gicquel ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Comparative Study ◽  
Dna Cleavage ◽  
Gel Electrophoresis ◽  
Force Microscopy ◽  
Atomic Force
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