scholarly journals Robust and artifact-free mounting of tissue samples for atomic force microscopy

BioTechniques ◽  
2014 ◽  
Vol 56 (1) ◽  
Author(s):  
Joshua T. Morgan ◽  
Vijay Krishna Raghunathan ◽  
Sara M. Thomasy ◽  
Christopher J. Murphy ◽  
Paul Russell
Keyword(s):  
Atomic Force Microscopy ◽  
Tissue Samples ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Test-retest reproducibility of atomic force microscopy measurements of human trabecular meshwork stiffness

2020 ◽  
Vol 2 (4) ◽  
pp. 34-43
Author(s):  
Larry Kagemann ◽  
Joe Candiello ◽  
Gadi Wollstein ◽  
Hiroshi Ishikawa ◽  
Richard A. Bilonick ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Trabecular Meshwork ◽  
Human Donor ◽  
Tissue Samples ◽  
Force Microscopy ◽  
Young’S Moduli ◽  
Atomic Force ◽  
Coefficients Of Variation ◽  
Cryostat Sections ◽  
Anterior Posterior

Purpose: The purpose of the present study was to quantify test-retest reproducibility of measurements of stiffness of the human trabecular meshwork (HTM) by atomic force microscopy (AFM). Methods: Eleven 40 μm radial limbal cryostat sections from a fresh human donor rim were mounted on charged slides and rehydrated at room temperature. Stiffness at four TM locations (anterior to posterior along Schlemm’s canal) was measured by AFM. At each location, a 6 x 6 grid was sampled. Indentation points were evenly distributed over a 20 μm x 20 μm area, with a rate of one load/unload cycle per second. Measurements were then repeated for calculation of test-retest variability. Results: The test-retest coefficients of variation for the four measurement locations (anterior to posterior) were 24.39, 25.28, 12.74, and 14.26%, respectively, with a notable drop in the two posterior locations compared to the anterior. The test-retest coefficient for the sections was 19.17%. For the entire eye, the test-retest coefficient of variation for the measurement of the TM stiffness was 17.13%. Young’s moduli consistently decreased from anterior to posterior location. Conclusions: Wide regional variation suggests that single value does little to fully describe the complex array of TM stiffness levels within the eye, and future studies of TM stiffness assessed by AFM should include multiple tissue samples from each eye, with documentation of the anterior-posterior location of each measurement.

scholarly journals Detecting Protein Aggregates on Untreated Human Tissue Samples by Atomic Force Microscopy Recognition Imaging

2010 ◽  
Vol 99 (5) ◽  
pp. 1660-1667 ◽  
Author(s):  
Rhiannon Creasey ◽  
Shiwani Sharma ◽  
Jamie E. Craig ◽  
Christopher T. Gibson ◽  
Andreas Ebner ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Human Tissue ◽  
Protein Aggregates ◽  
Tissue Samples ◽  
Force Microscopy ◽  
Atomic Force ◽  
Recognition Imaging

scholarly journals A cost-effective method to immobilize hydrated soft-tissue samples for atomic force microscopy

BioTechniques ◽  
2016 ◽  
Vol 61 (4) ◽  
Author(s):  
Suchit Sahai ◽  
Marysuna Wilkerson ◽  
Ana Maria Zaske ◽  
Scott D. Olson ◽  
Charles S. Cox ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Soft Tissue ◽  
Cost Effective ◽  
Tissue Samples ◽  
Force Microscopy ◽  
Atomic Force ◽  
Cost Effective Method

Electrostatic Forces on the Surface of Metals as Measured by Atomic Force Microscopy

2000 ◽  
Vol 10 (1-2) ◽  
pp. 15
Author(s):  
Eugene Sprague ◽  
Julio C. Palmaz ◽  
Cristina Simon ◽  
Aaron Watson
Keyword(s):  
Atomic Force Microscopy ◽  
Electrostatic Forces ◽  
Force Microscopy ◽  
Atomic Force
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