scholarly journals Magnetophoretic Behavior of 3T3 Cells Incubated with Saccharide-Coated MNPs

MRS Advances ◽  
2016 ◽  
Vol 2 (24) ◽  
pp. 1279-1284 ◽  
Author(s):  
Thomas W. Fallows ◽  
Thomas P. Coxon ◽  
Julie E. Gough ◽  
Simon J. Webb
Keyword(s):  
Cell Culture ◽  
Magnetic Nanoparticles ◽  
Magnetic Fields ◽  
Magnetite Nanoparticles ◽  
Fibroblast Cells ◽  
3T3 Cells ◽  
External Magnet ◽  
Coated Nanoparticles

ABSTRACTProviding magnetite nanoparticles with saccharide coatings has been found to significantly increase the interactions of the nanoparticles with cells. Glucose (Glc) or N-acetylglucosamine (GlcNAc) coated magnetic nanoparticles (MNPs) were used to magnetically label 3T3 fibroblast cells, and the response of the labelled cells to external magnetic fields was studied. It was found that cells incubated with Glc- or GlcNAc-coated nanoparticles were much more likely to move towards an external magnet than those incubated with uncoated nanoparticles. Furthermore, cells in suspension moved much faster than those in contact with the surface of polystyrene well plates, with stronger magnets increasing the speed of movement. Cells that were adhering to the floor of the cell culture well and did not move in the x-y plane could still be rotated about the z-axis by moving the external magnet around the cell.

scholarly journals Development of Antibody-Coated Magnetite Nanoparticles for Biomarker Immobilization

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Christian Chapa Gonzalez ◽  
Carlos A. Martínez Pérez ◽  
Alejandro Martínez Martínez ◽  
Imelda Olivas Armendáriz ◽  
Oscar Zavala Tapia ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Magnetic Separation ◽  
Magnetite Nanoparticles ◽  
Biomedical Applications ◽  
Polymeric Chain ◽  
Scfv Fragment ◽  
Enzyme Adsorption ◽  
Coated Nanoparticles ◽  
Detection Of Biomarkers ◽  
Biological Entities

Magnetic nanoparticles (MNPs) have great potential in biomedical applications because of their magnetic response offers the possibility to direct them to specific areas and target biological entities. Magnetic separation of biomolecules is one of the most important applications of MNPs because their versatility in detecting cancer biomarkers. However, the effectiveness of this method depends on many factors, including the type of functionalization onto MNPs. Therefore, in this study, magnetite nanoparticles have been developed in order to separate the 5′-nucleotidase enzyme (5eNT). The 5eNT is used as a bio-indicator for diagnosing diseases such as hepatic ischaemia, liver tumor, and hepatotoxic drugs damage. Magnetic nanoparticles were covered in a core/shell type with silica, aminosilane, and a double shell of silica-aminosilane. A ScFv (fragment antibody) and anti-CD73 antibody were attached to the coated nanoparticles in order to separate the enzyme. The magnetic separation of this enzyme with fragment antibody was found to be 28% higher than anti-CD73 antibody and the enzyme adsorption was improved with the double shell due to the increased length of the polymeric chain. Magnetite nanoparticles with a double shell (silica-aminosilane) were also found to be more sensitive than magnetite with a single shell in the detection of biomarkers.

Preparation Methods of Copper-Ferrocyanide Functionalized Magnetic Nanoparticles for Selective Removal of Cesium in Aqueous Solution

2013 ◽  
Author(s):  
Hee-Man Yang ◽  
Kune Woo Lee ◽  
Bum-Kyoung Seo ◽  
Jei Kwon Moon
Keyword(s):  
Aqueous Solution ◽  
Magnetic Nanoparticles ◽  
Magnetite Nanoparticles ◽  
Selective Removal ◽  
Radioactive Cesium ◽  
Preparation Methods ◽  
External Magnet ◽  
Copper Ferrocyanide ◽  
Functionalized Magnetic Nanoparticles

Copper ferrocyanide functionalized magnetite nanoparticles (Cu-FC-MNPs) were successfully synthesized by the immobilization of copper and ferrocyanide on the surface of [1-(2 amino-ethyl)-3-aminopropyl] trimethoxysilane modified magnetite nanoparticles. A radioactive cesium (Cs) adsorption test was carried out to investigate the effectiveness of Cu-FC-MNPS for the removal of radioactive Cs. Furthermore, the Cu-FC-MNPs showed excellent separation ability by an external magnet in an aqueous solution.

scholarly journals Kilham Polyomavirus: Activation of Gene Expression and DNA Replication in Mouse Fibroblast Cells by an Enhancer Substitution

2001 ◽  
Vol 75 (21) ◽  
pp. 10015-10023 ◽  
Author(s):  
Shouting Zhang ◽  
Göran Magnusson
Keyword(s):  
Gene Expression ◽  
Dna Replication ◽  
T Antigen ◽  
Mouse Fibroblast ◽  
Fibroblast Cells ◽  
Large T Antigen ◽  
Wild Type ◽  
3T3 Cells ◽  
Late Promoter ◽  
Substitution Mutant

ABSTRACT The Kilham strain of polyomavirus (KV) infects vascular endothelial cells in vivo (J. E. Greenlee, Infect. Immun. 26:705–713, 1979), but no permissive cell type for growth of the virus in vitro has been identified. The failure of KV DNA to replicate in mouse fibroblast cells after transfection suggested that viral gene expression had narrow cell specificity. A KV substitution mutant having a part of the regulatory region of KV DNA replaced with a segment of the polyomavirus transcriptional enhancer was constructed. The substitution mutant was able to replicate in transfected 3T3 cells, and the newly replicated viral DNA associated with protein to form particles with the density of virions in CsCl equilibrium gradients. However, these particles were noninfectious when tested on 3T3 cells, suggesting that absorption or uptake of virus particles was defective for these cells. Analysis of early and late promoter activities by luciferase reporter gene expression showed that the enhancer substitution had a moderate positive effect on early gene expression and a large effect on the expression of the late genes. KV large T antigen inhibited the activities of both the wild-type and the substitution mutant early promoter, whereas only the mutant late promoter was activated under the same conditions. A comparison of the KV and polyomavirus large T antigens showed that they were not interchangeable in the initiation of KV and polyomavirus DNA synthesis. Furthermore, the wild-type KV origin of DNA replication was less active than the mutant structure in the presence of saturating amounts of KV large T antigen. Together, our data demonstrate several differences between the two types of large T antigen in their interactions with cellular proteins.

scholarly journals Identification of natural products and their derivatives as promising inhibitors of protein glycation with non-toxic nature against mouse fibroblast 3T3 cells

2017 ◽  
Vol 8 (4) ◽  
pp. 533 ◽  
Author(s):  
Ghulam Abbas ◽  
Ahmed Suliman Al-Harrasi ◽  
Hidayat Hussain ◽  
Samina Abdul Sattar ◽  
M. Iqbal Choudhary
Keyword(s):  
Acetic Acid ◽  
Significant Inhibition ◽  
Mouse Fibroblast ◽  
Protein Glycation ◽  
Fibroblast Cells ◽  
Inhibition Activity ◽  
3T3 Cells ◽  
Gossypol Acetic Acid ◽  
Deoxypeganine Hydrochloride

<p>This study was performed to identify new inhibitors of protein glycation <em>in vitro</em>. Protein glycation is one of the major causes of late diabetic complications. In this study, terpenoids and alkaloids, isolated from different medicinal plants, along with their derivatives, were evaluated for their antiglycation activity <em>in vitro,</em> while MTT assay on mouse fibroblast 3T3 cells was used to assess their potential cytotoxicity. Among the tested compounds, gossypol (2,2′-<em>bis</em>-(formyl-1,6,7-trihydroxy-5-isopropyl-3-methylnaphthalene) (<strong>1</strong>), isolated from<em> Gossypium herbaceum, </em>and its derivatives,<em> </em>gossypol acetic acid (<strong>2</strong>), gossypolidene- 4-aminoantipyrine (<strong>4</strong>), and gazolidone (<strong>6</strong>), showed a potent antiglycation activity (IC<sub>50</sub> &lt; 16 <em>µ</em>M), while gossypolidene-4-aminoantipyrine (<strong>5</strong>) showed a significant antiglycation activity with IC<sub>50 </sub>value 82.934±2.924<em> µ</em>M, in BSA-fluorescence assay. Alkaloid, noscapine (3S)-6,7-Dimethoxy-3-[(5R)-4-methoxy-6-methyl-5,6,7,8-tetrahy-dro-1,3-dioxolo[4,5-g]isoquinolin-5-yl] isobenzofuran-1(3<em>H</em>)-one (<strong>7</strong>), isolated from <em>Papaver somniferum, N</em>-nitrosoaphyllinic acid (<strong>9</strong>), a derivative of alkaloid aphylline<em>, </em>and 2<em>H</em>-quinolizine, octahydro salt (<strong>11</strong>), a salt of alkaloid lupinine, exhibited significant inhibition activity with<em> </em>IC<sub>50 </sub>values 152.662±5.432, 393.758 ±4.001 µM and 110.203±4.816µM, respectively. Similarly, compounds<strong> </strong>gossypolidene thiocarbamide (<strong>3</strong>), deoxypeganine hydrochloride (<strong>8</strong>)<strong>, </strong>lupinine (<strong>10</strong>) and cytisine (<strong>12</strong>) showed moderate inhibition with IC<sub>50</sub> values of 401.865 ±18.450, 863.322 ±6.415, 712.176±7.745, and 728.462±2.331<em> </em>µM, respectively. The results were compared with the standard antiglycation agent, rutin (<strong>13</strong>) (IC<sub>50 </sub>=98.012±2.030 µM).</p>Cellular cytotoxicity assay showed only gossypol acetic acid (<strong>2</strong>) and gossypolidene thiocarbamide (<strong>3</strong>) as somewhat toxic to 3T3 (mouse fibroblast) cells with IC<sub>50 </sub>values<em> </em>2.07 ±0.61 and 5.00 ±1.89 µM, respectively. Cycloheximide was used as a standard in this assay with IC<sub>50</sub> value 0.3 ± 0.089 μM

Effect of Surface Charge on the Uptake of Magnetic Nanoparticles in Mouse Fibroblast Cells

2017 ◽  
Vol 8 ◽  
pp. 1-5 ◽  
Author(s):  
Jefunnie S. Matahum ◽  
Chao-Ming Su ◽  
Wei-Jie Wang ◽  
Shyh-Liang Lou ◽  
Tzong-Rong Ger
Keyword(s):  
Magnetic Nanoparticles ◽  
Surface Charge ◽  
Mouse Fibroblast ◽  
Fibroblast Cells ◽  
Effect Of Surface

Thermal Conductivity Enhancement of Room Temperature Ionic Liquids (RTILs) With Various Magnetic Nanoparticles

2012 ◽  
Author(s):  
N. Y. Jagath B. Nikapitiya ◽  
Hyejin Moon
Keyword(s):  
Thermal Conductivity ◽  
Ionic Liquids ◽  
Magnetic Nanoparticles ◽  
Magnetic Fields ◽  
Room Temperature ◽  
Particle Concentration ◽  
Thermal Conductivity Enhancement ◽  
Room Temperature Ionic Liquids ◽  
Conductivity Enhancement ◽  
Magnetic Nanofluids

This paper reports an experimental study of thermal conductivity of room temperature ionic liquids (RTILs) based magnetic nanofluids. Various magnetic nanoparticles of metal oxides with high thermal conductivity, such as CuO, Al2O3, Fe3O4 and Carbon Nano Tubes (CNTs), were used to prepare magnetic nanofluids, while RTIL, trihexyl (tetradecyl) posphonium dicyanamide was used as the base fluid. Two major parameters that affect to the thermal conductivity enhancement of fluids were investigated. The effect of particle concentration and external magnetic fields were tested. It was observed that the magnetic nanofluids thermal conductivities increase with increment of particle concentration and external magnetic field parallel to the temperature gradient. Besides, it was observed that under higher magnetic fields, thermal conductivity enhancement tends to approach a saturation state. Surfactant was used to disperse magnetic nanoparticles within the RTILs. The transient hot wire method was used for this investigation.

scholarly journals Role of zinc substitution in magnetic hyperthermia properties of magnetite nanoparticles: interplay between intrinsic properties and dipolar interactions

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Yaser Hadadian ◽  
Ana Paula Ramos ◽  
Theo Z. Pavan
Keyword(s):  
Magnetic Nanoparticles ◽  
Magnetite Nanoparticles ◽  
Linear Response Theory ◽  
Magnetic Hyperthermia ◽  
Superior Performance ◽  
Dipolar Interactions ◽  
Intrinsic Properties ◽  
Specific Loss ◽  
Heating Efficiency ◽  
Specific Loss Power

AbstractOptimizing the intrinsic properties of magnetic nanoparticles for magnetic hyperthermia is of considerable concern. In addition, the heating efficiency of the nanoparticles can be substantially influenced by dipolar interactions. Since adequate control of the intrinsic properties of magnetic nanoparticles is not straightforward, experimentally studying the complex interplay between these properties and dipolar interactions affecting the specific loss power can be challenging. Substituting zinc in magnetite structure is considered as an elegant approach to tune its properties. Here, we present experimental and numerical simulation results of magnetic hyperthermia studies using a series of zinc-substituted magnetite nanoparticles (ZnxFe1-xFe2O4, x = 0.0, 0.1, 0.2, 0.3 and 0.4). All experiments were conducted in linear regime and the results were inferred based on the numerical simulations conducted in the framework of the linear response theory. The results showed that depending on the nanoparticles intrinsic properties, interparticle interactions can have different effects on the specific loss power. When dipolar interactions were strong enough to affect the heating efficiency, the parameter σ = KeffV/kBT (Keff is the effective anisotropy and V the volume of the particles) determined the type of the effect. Finally, the sample x = 0.1 showed a superior performance with a relatively high intrinsic loss power 5.4 nHm2kg−1.

scholarly journals Effect of Geometrical Structure, Drying, and Synthetic Method on Aminated Chitosan-Coated Magnetic Nanoparticles Utility for HSA Effective Immobilization

Molecules ◽  
2019 ◽  
Vol 24 (10) ◽  
pp. 1925 ◽  
Author(s):  
Marta Ziegler-Borowska ◽  
Kinga Mylkie ◽  
Mariana Kozlowska ◽  
Pawel Nowak ◽  
Dorota Chelminiak-Dudkiewicz ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Synthesis Method ◽  
Pharmacokinetic Parameters ◽  
Drying Methods ◽  
Drying Method ◽  
Coated Nanoparticles ◽  
Immobilized Proteins ◽  
Effect Of The Support ◽  
Different Content ◽  
Active Substances

Human serum albumin (HSA) is one of the most frequently immobilized proteins on the surface of carriers, including magnetic nanoparticles. This is because the drug–HSA interaction study is one of the basic pharmacokinetic parameters determined for drugs. In spite of many works describing the immobilization of HSA and the binding of active substances, research describing the influence of the used support on the effectiveness of immobilization is missing. There are also no reports about the effect of the support drying method on the effectiveness of protein immobilization. This paper examines the effect of both the method of functionalizing the polymer coating covering magnetic nanoparticles (MNPs), and the drying methods for the immobilization of HSA. Albumin was immobilized on three types of aminated chitosan-coated nanoparticles with a different content of amino groups long distanced from the surface Fe3O4-CS-Et(NH2)1–3. The obtained results showed that both the synthesis method and the method of drying nanoparticles have a large impact on the effectiveness of immobilization. Due to the fact that the results obtained for Fe3O4-CS-Et(NH2)2 significantly differ from those obtained for the others, the influence of the geometry of the shell structure on the ability to bind HSA was also explained by molecular dynamics.

scholarly journals One-step synthesis of polyethyleneimine-coated magnetite nanoparticles and their structural, magnetic and power absorption study

RSC Advances ◽  
2020 ◽  
Vol 10 (68) ◽  
pp. 41807-41815
Author(s):  
Lizbet León Félix ◽  
Marco Antonio Rodriguez Martínez ◽  
David Gregorio Pacheco Salazar ◽  
José Antonio Huamani Coaquira
Keyword(s):  
Magnetic Nanoparticles ◽  
Magnetite Nanoparticles ◽  
Biomedical Applications ◽  
Cancer Imaging ◽  
Absorption Study ◽  
Power Absorption ◽  
Chemical Surface ◽  
One Step

Magnetic nanoparticles (NPs) are especially interesting for several biomedical applications due to their chemical surface, especially for targeted cancer imaging and therapeutics.

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