scholarly journals Development of Antibody-Coated Magnetite Nanoparticles for Biomarker Immobilization

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Christian Chapa Gonzalez ◽  
Carlos A. Martínez Pérez ◽  
Alejandro Martínez Martínez ◽  
Imelda Olivas Armendáriz ◽  
Oscar Zavala Tapia ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Magnetic Separation ◽  
Magnetite Nanoparticles ◽  
Biomedical Applications ◽  
Polymeric Chain ◽  
Scfv Fragment ◽  
Enzyme Adsorption ◽  
Coated Nanoparticles ◽  
Detection Of Biomarkers ◽  
Biological Entities

Magnetic nanoparticles (MNPs) have great potential in biomedical applications because of their magnetic response offers the possibility to direct them to specific areas and target biological entities. Magnetic separation of biomolecules is one of the most important applications of MNPs because their versatility in detecting cancer biomarkers. However, the effectiveness of this method depends on many factors, including the type of functionalization onto MNPs. Therefore, in this study, magnetite nanoparticles have been developed in order to separate the 5′-nucleotidase enzyme (5eNT). The 5eNT is used as a bio-indicator for diagnosing diseases such as hepatic ischaemia, liver tumor, and hepatotoxic drugs damage. Magnetic nanoparticles were covered in a core/shell type with silica, aminosilane, and a double shell of silica-aminosilane. A ScFv (fragment antibody) and anti-CD73 antibody were attached to the coated nanoparticles in order to separate the enzyme. The magnetic separation of this enzyme with fragment antibody was found to be 28% higher than anti-CD73 antibody and the enzyme adsorption was improved with the double shell due to the increased length of the polymeric chain. Magnetite nanoparticles with a double shell (silica-aminosilane) were also found to be more sensitive than magnetite with a single shell in the detection of biomarkers.

scholarly journals One-step synthesis of polyethyleneimine-coated magnetite nanoparticles and their structural, magnetic and power absorption study

RSC Advances ◽  
2020 ◽  
Vol 10 (68) ◽  
pp. 41807-41815
Author(s):  
Lizbet León Félix ◽  
Marco Antonio Rodriguez Martínez ◽  
David Gregorio Pacheco Salazar ◽  
José Antonio Huamani Coaquira
Keyword(s):  
Magnetic Nanoparticles ◽  
Magnetite Nanoparticles ◽  
Biomedical Applications ◽  
Cancer Imaging ◽  
Absorption Study ◽  
Power Absorption ◽  
Chemical Surface ◽  
One Step

Magnetic nanoparticles (NPs) are especially interesting for several biomedical applications due to their chemical surface, especially for targeted cancer imaging and therapeutics.

scholarly journals Biomedical applications of high gradient magnetic separation: progress towards therapeutic haeomofiltration

2015 ◽  
Vol 60 (5) ◽  
Author(s):  
George Frodsham ◽  
Quentin A. Pankhurst
Keyword(s):  
Magnetic Nanoparticles ◽  
Magnetic Separation ◽  
Biomedical Applications ◽  
State Of The Art ◽  
Specific Binding ◽  
Processing Industry ◽  
High Gradient Magnetic Separation ◽  
High Gradient ◽  
Binding Agents ◽  
Key Enabling Technologies

AbstractHigh gradient magnetic separation is a well-established technology in the mineral processing industry, and has been used for decades in the bioprocessing industry. Less well known is the increasing role that high gradient magnetic separation is playing in biomedical applications, for both diagnostic and therapeutic purposes. We review here the state of the art in this emerging field, with a focus on therapeutic haemofiltration, the key enabling technologies relating to the functionalisation of magnetic nanoparticles with target-specific binding agents, and the development of extra-corporeal circuits to enable the

scholarly journals Magnetophoretic Behavior of 3T3 Cells Incubated with Saccharide-Coated MNPs

MRS Advances ◽  
2016 ◽  
Vol 2 (24) ◽  
pp. 1279-1284 ◽  
Author(s):  
Thomas W. Fallows ◽  
Thomas P. Coxon ◽  
Julie E. Gough ◽  
Simon J. Webb
Keyword(s):  
Cell Culture ◽  
Magnetic Nanoparticles ◽  
Magnetic Fields ◽  
Magnetite Nanoparticles ◽  
Fibroblast Cells ◽  
3T3 Cells ◽  
External Magnet ◽  
Coated Nanoparticles

ABSTRACTProviding magnetite nanoparticles with saccharide coatings has been found to significantly increase the interactions of the nanoparticles with cells. Glucose (Glc) or N-acetylglucosamine (GlcNAc) coated magnetic nanoparticles (MNPs) were used to magnetically label 3T3 fibroblast cells, and the response of the labelled cells to external magnetic fields was studied. It was found that cells incubated with Glc- or GlcNAc-coated nanoparticles were much more likely to move towards an external magnet than those incubated with uncoated nanoparticles. Furthermore, cells in suspension moved much faster than those in contact with the surface of polystyrene well plates, with stronger magnets increasing the speed of movement. Cells that were adhering to the floor of the cell culture well and did not move in the x-y plane could still be rotated about the z-axis by moving the external magnet around the cell.

Functionalized magnetic nanoparticles for biomedical applications

2015 ◽  
Vol 21 (42) ◽  
pp. 6038-6054 ◽  
Author(s):  
Dragoș Gudovan ◽  
Paul Balaure ◽  
Dan Mihăiescu ◽  
Adrian Fudulu ◽  
Bogdan Purcăreanu ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Biomedical Applications ◽  
Functionalized Magnetic Nanoparticles

scholarly journals Interfacing aptamers, nanoparticles and graphene in a hierarchical structure for highly selective detection of biomolecules in OECT devices

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Carlotta Peruzzi ◽  
Silvia Battistoni ◽  
Daniela Montesarchio ◽  
Matteo Cocuzza ◽  
Simone Luigi Marasso ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
Au Nanoparticles ◽  
Limit Of Detection ◽  
High Sensitivity ◽  
Minimal Invasiveness ◽  
Hierarchic Structure ◽  
Final Response ◽  
Detection Of Biomolecules ◽  
Good Signal ◽  
Detection Of Biomarkers

AbstractIn several biomedical applications, the detection of biomarkers demands high sensitivity, selectivity and easy-to-use devices. Organic electrochemical transistors (OECTs) represent a promising class of devices combining a minimal invasiveness and good signal transduction. However, OECTs lack of intrinsic selectivity that should be implemented by specific approaches to make them well suitable for biomedical applications. Here, we report on a biosensor in which selectivity and a high sensitivity are achieved by interfacing, in an OECT architecture, a novel gate electrode based on aptamers, Au nanoparticles and graphene hierarchically organized to optimize the final response. The fabricated biosensor performs state of the art limit of detection monitoring biomolecules, such as thrombin-with a limit of detection in the picomolar range (≤ 5 pM) and a very good selectivity even in presence of supraphysiological concentrations of Bovine Serum Albumin (BSA-1mM). These accomplishments are the final result of the gate hierarchic structure that reduces sterich indrance that could contrast the recognition events and minimizes false positive, because of the low affinity of graphene towards the physiological environment. Since our approach can be easily applied to a large variety of different biomarkers, we envisage a relevant potential for a large series of different biomedical applications.

Simulated Clustering Dynamics of Colloidal Magnetic Nanoparticles

Nanoscale ◽  
2021 ◽  
Author(s):  
Frederik Laust Durhuus ◽  
Lau Halkier Wandall ◽  
Mathias Hoeg Boisen ◽  
Mathias Kure ◽  
Marco Beleggia ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Self Assembly ◽  
Biomedical Applications ◽  
Magnetic Nanoparticle ◽  
Bottom Up ◽  
Novel Materials ◽  
Nanoparticle Clusters ◽  
Clustering Dynamics

Magnetically guided self-assembly of nanoparticles is a promising bottom-up method to fabricate novel materials and superstructures, such as, for example, magnetic nanoparticle clusters for biomedical applications. The existence of assembled...

scholarly journals Modulation of Crystallinity through Radiofrequency Electromagnetic Fields in PLLA/Magnetic Nanoparticles Composites: A Proof of Concept

Materials ◽  
2021 ◽  
Vol 14 (15) ◽  
pp. 4300
Author(s):  
Marta Multigner ◽  
Irene Morales ◽  
Marta Muñoz ◽  
Victoria Bonache ◽  
Fernando Giacomone ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Magnetic Nanoparticles ◽  
Electromagnetic Fields ◽  
Biomedical Applications ◽  
Infrared Camera ◽  
Proof Of Concept ◽  
Scanning Calorimetry ◽  
Radiofrequency Electromagnetic Fields ◽  
Degradation Properties ◽  
Heat Released

To modulate the properties of degradable implants from outside of the human body represents a major challenge in the field of biomaterials. Polylactic acid is one of the most used polymers in biomedical applications, but it tends to lose its mechanical properties too quickly during degradation. In the present study, a way to reinforce poly-L lactic acid (PLLA) with magnetic nanoparticles (MNPs) that have the capacity to heat under radiofrequency electromagnetic fields (EMF) is proposed. As mechanical and degradation properties are related to the crystallinity of PLLA, the aim of the work was to explore the possibility of modifying the structure of the polymer through the heating of the reinforcing MNPs by EMF within the biological limit range f·H < 5·× 109 Am−1·s−1. Composites were prepared by dispersing MNPs under sonication in a solution of PLLA. The heat released by the MNPs was monitored by an infrared camera and changes in the polymer were analyzed with differential scanning calorimetry and nanoindentation techniques. The crystallinity, hardness, and elastic modulus of nanocomposites increase with EMF treatment.

Magnetic Nanoparticles for Efficient Removal of Oilfield “Contaminants": Modeling of Magnetic Separation and Validation

2015 ◽  
Author(s):  
Valentina Prigiobbe ◽  
Saebom Ko ◽  
Qing Wang ◽  
Chun Huh ◽  
Steven L. Bryant ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Magnetic Separation ◽  
Efficient Removal
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