Photonic Crystal Optical Tweezers for Living Cells

2017 ◽  
Author(s):  
Lih Y. Lin ◽  
Peifeng Jing ◽  
Ethan Keeler
Keyword(s):  
Photonic Crystal ◽  
Optical Tweezers ◽  
Living Cells

Large Deformation of Biological Cells by Optical Tweezers

2003 ◽  
Author(s):  
S. Suresh ◽  
C. T. Lim ◽  
M. Dao
Keyword(s):  
Optical Tweezers ◽  
Mechanical Test ◽  
Living Cells ◽  
Test Methods ◽  
Mechanical Forces ◽  
Deformation Characteristics ◽  
Biological Cells ◽  
Whole Cells ◽  
Aspiration Technique ◽  
Stress States

The chemical and biological functions of living cells are known to be influenced strongly by mechanical forces and deformation, and the ability of cells to detect and support forces, in turn, is also affected by chemical and biological factors. Furthermore, the progression of a number of inherited and infectious diseases have also been identified to have a strong correlation with the mechanical deformation characteristics of biological cells. Consequently, the deformation characteristics of whole cells and cell membranes have long been investigated using a variety of experimental methods, such as the micropipette aspiration technique, and by computational modeling (see, for example, refs. [1, 2]). Recent advances in experimental techniques capable of probing mechanical forces and displacements to a resolution of picoNewton and nanometer, respectively, have facilitated use of mechanical test methods for living cells whereby precise measurements of response under different stress states could be investigated.

Using optical tweezers to investigate the specific single-interaction between apoA-I molecule and ABCA1 on living cells

2013 ◽  
Vol 11 (9) ◽  
pp. 091701-91704 ◽  
Author(s):  
Jie Yu Jie Yu ◽  
Xunliang Tong Xunliang Tong ◽  
Chengbin Li Chengbin Li ◽  
Yining Huang Yining Huang ◽  
Anpei Ye Anpei Ye
Keyword(s):  
Optical Tweezers ◽  
Living Cells

scholarly journals Bio-Molecular Applications of Recent Developments in Optical Tweezers

Biomolecules ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 23 ◽  
Author(s):  
Dhawal Choudhary ◽  
Alessandro Mossa ◽  
Milind Jadhav ◽  
Ciro Cecconi
Keyword(s):  
Photonic Crystal ◽  
Optical Tweezers ◽  
Single Molecule ◽  
Continuous Wave ◽  
Imaging Techniques ◽  
Resolving Power ◽  
Optical Traps ◽  
Laser Source ◽  
One Dimensional

In the past three decades, the ability to optically manipulate biomolecules has spurred a new era of medical and biophysical research. Optical tweezers (OT) have enabled experimenters to trap, sort, and probe cells, as well as discern the structural dynamics of proteins and nucleic acids at single molecule level. The steady improvement in OT’s resolving power has progressively pushed the envelope of their applications; there are, however, some inherent limitations that are prompting researchers to look for alternatives to the conventional techniques. To begin with, OT are restricted by their one-dimensional approach, which makes it difficult to conjure an exhaustive three-dimensional picture of biological systems. The high-intensity trapping laser can damage biological samples, a fact that restricts the feasibility of in vivo applications. Finally, direct manipulation of biological matter at nanometer scale remains a significant challenge for conventional OT. A significant amount of literature has been dedicated in the last 10 years to address the aforementioned shortcomings. Innovations in laser technology and advances in various other spheres of applied physics have been capitalized upon to evolve the next generation OT systems. In this review, we elucidate a few of these developments, with particular focus on their biological applications. The manipulation of nanoscopic objects has been achieved by means of plasmonic optical tweezers (POT), which utilize localized surface plasmons to generate optical traps with enhanced trapping potential, and photonic crystal optical tweezers (PhC OT), which attain the same goal by employing different photonic crystal geometries. Femtosecond optical tweezers (fs OT), constructed by replacing the continuous wave (cw) laser source with a femtosecond laser, promise to greatly reduce the damage to living samples. Finally, one way to transcend the one-dimensional nature of the data gained by OT is to couple them to the other large family of single molecule tools, i.e., fluorescence-based imaging techniques. We discuss the distinct advantages of the aforementioned techniques as well as the alternative experimental perspective they provide in comparison to conventional OT.

scholarly journals Photonic Crystal Optical Tweezers with High Efficiency for Live Biological Samples and Viability Characterization

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Peifeng Jing ◽  
Jingda Wu ◽  
Gary W. Liu ◽  
Ethan G. Keeler ◽  
Suzie H. Pun ◽  
...  
Keyword(s):  
Photonic Crystal ◽  
Optical Tweezers ◽  
Biological Samples ◽  
High Efficiency

Fluorescent II−VI Semiconductor Quantum Dots in Living Cells:  Nonlinear Microspectroscopy in an Optical Tweezers System

2008 ◽  
Vol 112 (9) ◽  
pp. 2734-2737 ◽  
Author(s):  
Patricia M. A. Farias ◽  
Beate S. Santos ◽  
André A. de Thomaz ◽  
Ricardo Ferreira ◽  
Frederico D. Menezes ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Optical Tweezers ◽  
Living Cells ◽  
Semiconductor Quantum Dots

2D multiforce optical tweezers to investigate cell adhesion strengthening in living cells

2006 ◽  
Author(s):  
Valentina Emiliani ◽  
Daniele Sanvitto ◽  
Christiane Durieux
Keyword(s):  
Cell Adhesion ◽  
Optical Tweezers ◽  
Living Cells

HACF-based optical tweezers available for living cells manipulating and sterile transporting

2018 ◽  
Vol 427 ◽  
pp. 563-566 ◽  
Author(s):  
Yu Zhang ◽  
Yan Li ◽  
Yaxun Zhang ◽  
Chuanzhen Hu ◽  
Zhihai Liu ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Living Cells

scholarly journals Probing Ligand-Receptor Interaction in Living Cells Using Force Measurements With Optical Tweezers

2020 ◽  
Vol 8 ◽  
Author(s):  
Carolin Riesenberg ◽  
Christian Alejandro Iriarte-Valdez ◽  
Annegret Becker ◽  
Maria Dienerowitz ◽  
Alexander Heisterkamp ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Living Cells ◽  
Receptor Interaction ◽  
Force Measurements
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