scholarly journals Probing Ligand-Receptor Interaction in Living Cells Using Force Measurements With Optical Tweezers

2020 ◽  
Vol 8 ◽  
Author(s):  
Carolin Riesenberg ◽  
Christian Alejandro Iriarte-Valdez ◽  
Annegret Becker ◽  
Maria Dienerowitz ◽  
Alexander Heisterkamp ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Living Cells ◽  
Receptor Interaction ◽  
Force Measurements

scholarly journals Force measurements with optical tweezers inside living cells

2014 ◽  
Author(s):  
Josep Mas ◽  
Arnau Farré ◽  
Jordi Sancho-Parramon ◽  
Estela Martín-Badosa ◽  
Mario Montes-Usategui
Keyword(s):  
Optical Tweezers ◽  
Living Cells ◽  
Force Measurements

Large Deformation of Biological Cells by Optical Tweezers

2003 ◽  
Author(s):  
S. Suresh ◽  
C. T. Lim ◽  
M. Dao
Keyword(s):  
Optical Tweezers ◽  
Mechanical Test ◽  
Living Cells ◽  
Test Methods ◽  
Mechanical Forces ◽  
Deformation Characteristics ◽  
Biological Cells ◽  
Whole Cells ◽  
Aspiration Technique ◽  
Stress States

The chemical and biological functions of living cells are known to be influenced strongly by mechanical forces and deformation, and the ability of cells to detect and support forces, in turn, is also affected by chemical and biological factors. Furthermore, the progression of a number of inherited and infectious diseases have also been identified to have a strong correlation with the mechanical deformation characteristics of biological cells. Consequently, the deformation characteristics of whole cells and cell membranes have long been investigated using a variety of experimental methods, such as the micropipette aspiration technique, and by computational modeling (see, for example, refs. [1, 2]). Recent advances in experimental techniques capable of probing mechanical forces and displacements to a resolution of picoNewton and nanometer, respectively, have facilitated use of mechanical test methods for living cells whereby precise measurements of response under different stress states could be investigated.

scholarly journals Hemodynamic forces can be accurately measured in vivo with optical tweezers

2017 ◽  
Author(s):  
Sébastien Harlepp ◽  
Fabrice Thalmann ◽  
Gautier Follain ◽  
Jacky G. Goetz
Keyword(s):  
Optical Tweezers ◽  
Cell Biology ◽  
Accurate Determination ◽  
Force Measurements ◽  
Hemodynamic Forces ◽  
Non Invasive ◽  
Drag Forces ◽  
Measured Flow ◽  
Living Organisms

AbstractForce sensing and generation at the tissular and cellular scale is central to many biological events. There is a growing interest in modern cell biology for methods enabling force measurements in vivo. Optical trapping allows non-invasive probing of pico-Newton forces and thus emerged as a promising mean for assessing biomechanics in vivo. Nevertheless, the main obstacles rely in the accurate determination of the trap stiffness in heterogeneous living organisms, at any position where the trap is used. A proper calibration of the trap stiffness is thus required for performing accurate and reliable force measurements in vivo. Here, we introduce a method that overcomes these difficulties by accurately measuring hemodynamic profiles in order to calibrate the trap stiffness. Doing so, and using numerical methods to assess the accuracy of the experimental data, we measured flow profiles and drag forces imposed to trapped red blood cells of living zebrafish embryos. Using treatments enabling blood flow tuning, we demonstrated that such method is powerful in measuring hemodynamic forces in vivo with accuracy and confidence. Altogether, this study demonstrates the power of optical tweezing in measuring low range hemodynamic forces in vivo and offers an unprecedented tool in both cell and developmental biology.

Using optical tweezers to investigate the specific single-interaction between apoA-I molecule and ABCA1 on living cells

2013 ◽  
Vol 11 (9) ◽  
pp. 091701-91704 ◽  
Author(s):  
Jie Yu Jie Yu ◽  
Xunliang Tong Xunliang Tong ◽  
Chengbin Li Chengbin Li ◽  
Yining Huang Yining Huang ◽  
Anpei Ye Anpei Ye
Keyword(s):  
Optical Tweezers ◽  
Living Cells

Dynamic tether force measurements of the outer hair cells plasma membrane with optical tweezers

2001 ◽  
Author(s):  
Bahman Anvari ◽  
Zhiwei Li ◽  
Masayoshi Takashima ◽  
Peter Brecht ◽  
Jorge H. Torres ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Optical Tweezers ◽  
Hair Cells ◽  
Outer Hair Cells ◽  
Force Measurements
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