2D multiforce optical tweezers to investigate cell adhesion strengthening in living cells

2006 ◽  
Author(s):  
Valentina Emiliani ◽  
Daniele Sanvitto ◽  
Christiane Durieux
Keyword(s):  
Cell Adhesion ◽  
Optical Tweezers ◽  
Living Cells

Large Deformation of Biological Cells by Optical Tweezers

2003 ◽  
Author(s):  
S. Suresh ◽  
C. T. Lim ◽  
M. Dao
Keyword(s):  
Optical Tweezers ◽  
Mechanical Test ◽  
Living Cells ◽  
Test Methods ◽  
Mechanical Forces ◽  
Deformation Characteristics ◽  
Biological Cells ◽  
Whole Cells ◽  
Aspiration Technique ◽  
Stress States

The chemical and biological functions of living cells are known to be influenced strongly by mechanical forces and deformation, and the ability of cells to detect and support forces, in turn, is also affected by chemical and biological factors. Furthermore, the progression of a number of inherited and infectious diseases have also been identified to have a strong correlation with the mechanical deformation characteristics of biological cells. Consequently, the deformation characteristics of whole cells and cell membranes have long been investigated using a variety of experimental methods, such as the micropipette aspiration technique, and by computational modeling (see, for example, refs. [1, 2]). Recent advances in experimental techniques capable of probing mechanical forces and displacements to a resolution of picoNewton and nanometer, respectively, have facilitated use of mechanical test methods for living cells whereby precise measurements of response under different stress states could be investigated.

Using optical tweezers to investigate the specific single-interaction between apoA-I molecule and ABCA1 on living cells

2013 ◽  
Vol 11 (9) ◽  
pp. 091701-91704 ◽  
Author(s):  
Jie Yu Jie Yu ◽  
Xunliang Tong Xunliang Tong ◽  
Chengbin Li Chengbin Li ◽  
Yining Huang Yining Huang ◽  
Anpei Ye Anpei Ye
Keyword(s):  
Optical Tweezers ◽  
Living Cells

Fluorescent II−VI Semiconductor Quantum Dots in Living Cells:  Nonlinear Microspectroscopy in an Optical Tweezers System

2008 ◽  
Vol 112 (9) ◽  
pp. 2734-2737 ◽  
Author(s):  
Patricia M. A. Farias ◽  
Beate S. Santos ◽  
André A. de Thomaz ◽  
Ricardo Ferreira ◽  
Frederico D. Menezes ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Optical Tweezers ◽  
Living Cells ◽  
Semiconductor Quantum Dots

HACF-based optical tweezers available for living cells manipulating and sterile transporting

2018 ◽  
Vol 427 ◽  
pp. 563-566 ◽  
Author(s):  
Yu Zhang ◽  
Yan Li ◽  
Yaxun Zhang ◽  
Chuanzhen Hu ◽  
Zhihai Liu ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Living Cells

scholarly journals Probing Ligand-Receptor Interaction in Living Cells Using Force Measurements With Optical Tweezers

2020 ◽  
Vol 8 ◽  
Author(s):  
Carolin Riesenberg ◽  
Christian Alejandro Iriarte-Valdez ◽  
Annegret Becker ◽  
Maria Dienerowitz ◽  
Alexander Heisterkamp ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Living Cells ◽  
Receptor Interaction ◽  
Force Measurements

scholarly journals Growth Process and Mechanics of Cell Adhesion Investigated by Optical Tweezers

2010 ◽  
Vol 98 (3) ◽  
pp. 728a
Author(s):  
Hideaki Miyoshi ◽  
Tadao Sugiura ◽  
Kotaro Minato
Keyword(s):  
Cell Adhesion ◽  
Optical Tweezers ◽  
Growth Process

Automated Capillary-Based Vacuum Pulse-Assisted Instrument for Single-Cell Acquisition and Concurrent Detachment/Adhesion Assay, A-picK

2021 ◽  
pp. 247263032098721
Author(s):  
David Ma ◽  
Zhongcai Ma ◽  
Lili C. Kudo ◽  
Stanislav L. Karsten
Keyword(s):  
Cell Adhesion ◽  
Single Cell ◽  
Optical Tweezers ◽  
Time Course ◽  
Cancer Metastasis ◽  
Large Body ◽  
A549 Cells ◽  
Molecular Profile ◽  
Adhesion Assay ◽  
Adhesion Properties

A large body of evidence points to the importance of cell adhesion molecules in cancer metastasis. Alterations in adhesion and attachment properties of neoplastic cells are important biomarkers of the metastatic potential of cancer. Loss of intracellular adhesion is correlated with more invasive phenotype by increasing the chances of malignant cells escaping from their site of origin, promoting metastasis. Therefore, there is great demand for rapid and accurate measurements of individual cell adhesion and attachment. Current technologies that measure adhesion properties in either suspension or bulk (microfluidics) remain very complex (e.g., atomic force microscopy [AFM], optical tweezers). Moreover, existing tools cannot provide measurements for fully attached individual adherent cells as they operate outside of such a force range. Even more importantly, none of the existing approaches permit concurrent and automated single-cell adhesion measurement and collection, which prohibits direct correlation between single-cell adhesion properties and molecular profile. Here, we report a fully automated and versatile platform, A-picK, that offers single-cell adhesion assay and isolation in parallel. We demonstrate the use of this approach for a time course analysis of human lung carcinoma A549 cells and substrate-specific adhesion potential using seven different substrates, including fibronectin, laminin, poly-l-lysine, carboxyl, amine, collagen, and gelatin.

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