High sensitivity of second harmonic generation from styryl dyes to cell membrane potential

2003 ◽  
Author(s):  
Andrew C. Millard ◽  
Lei Jin ◽  
Aaron Lewis ◽  
Leslie M. Loew
Keyword(s):  
Membrane Potential ◽  
Cell Membrane ◽  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
High Sensitivity ◽  
Cell Membrane Potential ◽  
Styryl Dyes

scholarly journals Second harmonic generation correlation spectroscopy for characterizing translationally diffusing protein nanocrystals

2016 ◽  
Vol 72 (7) ◽  
pp. 849-859
Author(s):  
Ximeng Y. Dow ◽  
Christopher M. Dettmar ◽  
Emma L. DeWalt ◽  
Justin A. Newman ◽  
Alexander R. Dow ◽  
...  
Keyword(s):  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Unit Volume ◽  
Second Harmonic ◽  
Incident Beam ◽  
High Sensitivity ◽  
Correlation Spectroscopy ◽  
Optical Process ◽  
Active Nanoparticles ◽  
Low Sensitivity

Second harmonic generation correlation spectroscopy (SHG-CS) is demonstrated as a new approach to protein nanocrystal characterization. A novel line-scanning approach was performed to enable autocorrelation analysis without sample damage from the intense incident beam. An analytical model for autocorrelation was developed, which includes a correction for the optical scattering forces arising when focusing intense, infrared beams. SHG-CS was applied to the analysis of BaTiO3nanoparticles ranging from 200 to ∼500 nm and of photosystem I nanocrystals. A size distribution was recovered for each sample and compared with the size histogram measured by scanning electron microscopy (SEM). Good agreement was observed between the two independent measurements. The intrinsic selectivity of the second-order nonlinear optical process provides SHG-CS with the ability to distinguish well ordered nanocrystals from conglomerates and amorphous aggregates. Combining the recovered distribution of particle diameters with the histogram of measured SHG intensities provides the inherent hyperpolarizability per unit volume of the SHG-active nanoparticles. Simulations suggest that the SHG activity per unit volume is likely to exhibit relatively low sensitivity to the subtle distortions within the lattice that contribute to resolution loss in X-ray diffraction, but high sensitivity to the presence of multi-domain crystals.

scholarly journals Second-Harmonic Generation Imaging of Membrane Potential with Photon Counting

2008 ◽  
Vol 14 (6) ◽  
pp. 526-531 ◽  
Author(s):  
Jiang Jiang ◽  
Rafael Yuste
Keyword(s):  
Membrane Potential ◽  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Photon Counting ◽  
Analog Voltage ◽  
Second Harmonic Generation Imaging ◽  
Kinetics Of ◽  
Small Voltage ◽  
Pulse Variation

AbstractSecond-harmonic generation (SHG) can be used for imaging membrane potential in neurons, but poor signal-to-noise (S/N) limits accurate measurements of small voltage transients. We use photon counting to improve the S/N of weak SHG signal detection. Photon counting generates shot-noise limited and integrable signals, eliminates pulse-to-pulse variation, and built-in discriminators reduces the background to practically zero. In single trials, by using photon counting, we obtain a more than a twofold S/N increase over analog voltage detection. Trial-to-trial variability is also reduced by 50%. Finally, we show that, using photon counting, the kinetics of fast events such as action potentials can be recorded more accurately.

Imaging Membrane Potential with Second Harmonic Generation Microscopy

2004 ◽  
Vol 10 (S02) ◽  
pp. 224-225 ◽  
Author(s):  
Boaz A Nemet ◽  
Volodymyr Nikolenko ◽  
Jiang Jiang ◽  
Rafael Yuste
Keyword(s):  
Membrane Potential ◽  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Second Harmonic Generation Microscopy

Extended abstract of a paper presented at Microscopy and Microanalysis 2004 in Savannah, Georgia, USA, August 1–5, 2004.

scholarly journals Label-free imaging of membrane potentials by intramembrane field modulation, assessed by Second Harmonic Generation Microscopy

2021 ◽  
Author(s):  
Yovan de Coene ◽  
Stijn Jooken ◽  
Olivier Deschaume ◽  
Valérie Van Steenbergen ◽  
Pieter Vanden Berghe ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Hek293t Cell ◽  
Cellular Function ◽  
Membrane Voltage ◽  
Live Cells ◽  
Voltage Sensitivity ◽  
Label Free

Optical interrogation of cellular electrical activity has proven itself essential for understanding cellular function and communication in complex networks. Voltage-sensitive dyes are important tools for assessing excitability but these highly lipophilic sensors may affect cellular function. Label-free techniques offer a major advantage as they eliminate the need for these external probes. In this work, we show that endogenous second harmonic generation (SHG) from live cells is highly sensitive to changes in membrane potential. Simultaneous electrophysiological control of a living (HEK293T) cell, through whole-cell voltage clamp reveals a linear relation between the SHG intensity and membrane voltage. Our results suggest that due to the high ionic strengths and fast optical response of biofluids, membrane hydration is not the main contributor to the observed field sensitivity. We further provide a conceptual framework that indicates that the SHG voltage sensitivity reflects the electric field within the biological asymmetric lipid bilayer owing to a nonzero χeff(2) tensor. Changing the membrane potential without surface modifications such as electrolyte screening offers high optical sensitivity to membrane voltage (≈40% per 100 mV), indicating the power of SHG for label-free read-out. These results hold promise for the design of a non-invasive label-free read-out tool for electrogenic cells.

scholarly journals Interface Driven Effects in Magnetization-Induced Optical Second Harmonic Generation in Layered Films Composed of Ferromagnetic and Heavy Metals

Materials ◽  
2021 ◽  
Vol 14 (13) ◽  
pp. 3573
Author(s):  
Evgeniy Mamonov ◽  
Irina Kolmychek ◽  
Victoria Radovskaya ◽  
Igor Pashen’kin ◽  
Nikita Gusev ◽  
...  
Keyword(s):  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Experimental Studies ◽  
High Sensitivity ◽  
Magnetization Distribution ◽  
Optical Second Harmonic Generation ◽  
Optical Effects ◽  
Non Local ◽  
The Magnetic Field

Properties of nanolayers can substantially differ from those of bulky materials, in part due to pronounced interface effects. It is known that combinations of layers of heavy and ferromagnetic metals leads to the appearance of specific spin textures induced by interface-induced Dzyaloshinskyi–Moria interaction (DMI), which attracts much interest and requires further studies. In this paper, we study magneto-optical effects in two- and three-layer films composed of a few nanometer thick Co layer adjacent to nanofilms of non-magnetic materials (Pt, W, Cu, Ta, MgO). For experimental studies of the interface magnetization-induced effects, we used the optical second harmonic generation (SHG) technique known for its high sensitivity to the symmetry breaking. We found that the structural asymmetry leads to the increase of the averaged SHG intensity, as well as to the magnetic field-induced effects in SHG. Moreover, by choosing the proper geometry of the experiment, we excluded the most studied linear in magnetization SHG contributions and, thus, succeeded in studying higher order in magnetization and non-local magnetic effects. We revealed odd in magnetization SHG effects consistent with the phenomenological description involving inhomogeneous (gradient) magnetization distribution at interfaces and found them quite pronounced, so that they should be necessarily taken into account when analyzing the non-linear magneto-optical response of nanostructures.

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