scholarly journals Membrane potential dynamics of axons in cultured hippocampal neurons probed by second-harmonic-generation imaging

2010 ◽  
Vol 15 (2) ◽  
pp. 020503 ◽  
Author(s):  
Mutsuo Nuriya ◽  
Masato Yasui
Keyword(s):  
Membrane Potential ◽  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Hippocampal Neurons ◽  
Second Harmonic ◽  
Second Harmonic Generation Imaging ◽  
Cultured Hippocampal Neurons

scholarly journals Second-Harmonic Generation Imaging of Membrane Potential with Photon Counting

2008 ◽  
Vol 14 (6) ◽  
pp. 526-531 ◽  
Author(s):  
Jiang Jiang ◽  
Rafael Yuste
Keyword(s):  
Membrane Potential ◽  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Photon Counting ◽  
Analog Voltage ◽  
Second Harmonic Generation Imaging ◽  
Kinetics Of ◽  
Small Voltage ◽  
Pulse Variation

AbstractSecond-harmonic generation (SHG) can be used for imaging membrane potential in neurons, but poor signal-to-noise (S/N) limits accurate measurements of small voltage transients. We use photon counting to improve the S/N of weak SHG signal detection. Photon counting generates shot-noise limited and integrable signals, eliminates pulse-to-pulse variation, and built-in discriminators reduces the background to practically zero. In single trials, by using photon counting, we obtain a more than a twofold S/N increase over analog voltage detection. Trial-to-trial variability is also reduced by 50%. Finally, we show that, using photon counting, the kinetics of fast events such as action potentials can be recorded more accurately.

scholarly journals Second-Harmonic Generation Imaging of Membrane Potential with Retinal Analogues

2011 ◽  
Vol 100 (1) ◽  
pp. 232-242 ◽  
Author(s):  
Patrick Theer ◽  
Winfried Denk ◽  
Mordechai Sheves ◽  
Aaron Lewis ◽  
Peter B. Detwiler
Keyword(s):  
Membrane Potential ◽  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Retinal Analogues ◽  
Second Harmonic Generation Imaging

scholarly journals Second harmonic generation imaging of fascia within thick tissue block

2007 ◽  
Vol 15 (12) ◽  
pp. 7296 ◽  
Author(s):  
Christian P. Pfeffer ◽  
Bjorn R. Olsen ◽  
François Légaré
Keyword(s):  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Tissue Block ◽  
Second Harmonic Generation Imaging

scholarly journals Second-harmonic generation imaging analysis can help distinguish sarcoidosis from tuberculoid leprosy

2018 ◽  
Vol 23 (12) ◽  
pp. 1
Author(s):  
Fabiane Leonel Utino ◽  
Marina Garcia ◽  
Paulo Eduardo Neves Ferreira Velho ◽  
Andréa Fernandes Eloy da Costa França ◽  
Rafael Fantelli Stelini ◽  
...  
Keyword(s):  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Imaging Analysis ◽  
Tuberculoid Leprosy ◽  
Second Harmonic Generation Imaging
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