Membrane potential detection with second-harmonic generation and two-photon excited fluorescence: A theoretical comparison

2006 ◽  
Vol 258 (2) ◽  
pp. 203-209 ◽  
Author(s):  
Thomas Pons ◽  
Jerome Mertz
Keyword(s):  
Membrane Potential ◽  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Two Photon ◽  
Theoretical Comparison

Extracting diagnostic stromal organization features based on intrinsic two-photon excited fluorescence and second-harmonic generation signals

2009 ◽  
Vol 14 (2) ◽  
pp. 020503 ◽  
Author(s):  
Shuangmu Zhuo ◽  
Jianxin Chen ◽  
Shusen Xie ◽  
Zhibin Hong ◽  
Xingshan Jiang
Keyword(s):  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Two Photon

scholarly journals Multimodal two-photon imaging using a second harmonic generation-specific dye

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Mutsuo Nuriya ◽  
Shun Fukushima ◽  
Atsuya Momotake ◽  
Takanori Shinotsuka ◽  
Masato Yasui ◽  
...  
Keyword(s):  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging

scholarly journals Fibro-C-Index: comprehensive, morphology-based quantification of liver fibrosis using second harmonic generation and two-photon microscopy

2009 ◽  
Vol 14 (4) ◽  
pp. 044013 ◽  
Author(s):  
Dean C. S. Tai ◽  
Nancy Tan ◽  
Shuoyu Xu ◽  
Chiang Huen Kang ◽  
Ser Mien Chia ◽  
...  
Keyword(s):  
Liver Fibrosis ◽  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Second-Harmonic Generation Imaging of Membrane Potential with Photon Counting

2008 ◽  
Vol 14 (6) ◽  
pp. 526-531 ◽  
Author(s):  
Jiang Jiang ◽  
Rafael Yuste
Keyword(s):  
Membrane Potential ◽  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Photon Counting ◽  
Analog Voltage ◽  
Second Harmonic Generation Imaging ◽  
Kinetics Of ◽  
Small Voltage ◽  
Pulse Variation

AbstractSecond-harmonic generation (SHG) can be used for imaging membrane potential in neurons, but poor signal-to-noise (S/N) limits accurate measurements of small voltage transients. We use photon counting to improve the S/N of weak SHG signal detection. Photon counting generates shot-noise limited and integrable signals, eliminates pulse-to-pulse variation, and built-in discriminators reduces the background to practically zero. In single trials, by using photon counting, we obtain a more than a twofold S/N increase over analog voltage detection. Trial-to-trial variability is also reduced by 50%. Finally, we show that, using photon counting, the kinetics of fast events such as action potentials can be recorded more accurately.

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