Time-dependence of the voltage-sensitivity of second harmonic generation from Styryl dyes

2006 ◽  
Author(s):  
Andrew C. Millard ◽  
Lei Jin ◽  
Leslie M. Loew
Keyword(s):  
Second Harmonic Generation ◽  
Time Dependence ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Voltage Sensitivity ◽  
Styryl Dyes

scholarly journals Label-free imaging of membrane potentials by intramembrane field modulation, assessed by Second Harmonic Generation Microscopy

2021 ◽  
Author(s):  
Yovan de Coene ◽  
Stijn Jooken ◽  
Olivier Deschaume ◽  
Valérie Van Steenbergen ◽  
Pieter Vanden Berghe ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Hek293t Cell ◽  
Cellular Function ◽  
Membrane Voltage ◽  
Live Cells ◽  
Voltage Sensitivity ◽  
Label Free

Optical interrogation of cellular electrical activity has proven itself essential for understanding cellular function and communication in complex networks. Voltage-sensitive dyes are important tools for assessing excitability but these highly lipophilic sensors may affect cellular function. Label-free techniques offer a major advantage as they eliminate the need for these external probes. In this work, we show that endogenous second harmonic generation (SHG) from live cells is highly sensitive to changes in membrane potential. Simultaneous electrophysiological control of a living (HEK293T) cell, through whole-cell voltage clamp reveals a linear relation between the SHG intensity and membrane voltage. Our results suggest that due to the high ionic strengths and fast optical response of biofluids, membrane hydration is not the main contributor to the observed field sensitivity. We further provide a conceptual framework that indicates that the SHG voltage sensitivity reflects the electric field within the biological asymmetric lipid bilayer owing to a nonzero χeff(2) tensor. Changing the membrane potential without surface modifications such as electrolyte screening offers high optical sensitivity to membrane voltage (≈40% per 100 mV), indicating the power of SHG for label-free read-out. These results hold promise for the design of a non-invasive label-free read-out tool for electrogenic cells.

scholarly journals Sensitivity of Second Harmonic Generation from Styryl Dyes to Transmembrane Potential

2004 ◽  
Vol 86 (2) ◽  
pp. 1169-1176 ◽  
Author(s):  
Andrew C. Millard ◽  
Lei Jin ◽  
Mei-de Wei ◽  
Joseph P. Wuskell ◽  
Aaron Lewis ◽  
...  
Keyword(s):  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Transmembrane Potential ◽  
Second Harmonic ◽  
Styryl Dyes

Test of directional photoionisation models of second harmonic generation in optical fibres

1992 ◽  
Vol 139 (2) ◽  
pp. 133 ◽  
Author(s):  
N.M. Lawandy ◽  
T.J. Driscoll ◽  
C.L. Adler ◽  
N.M. Lawandy
Keyword(s):  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Optical Fibres
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