Perturbative theory of the electro-optic response of second-harmonic generation membrane potential sensors

2002 ◽  
Author(s):  
Jerome Mertz ◽  
Laurent Moreaux ◽  
T. Pons
Keyword(s):  
Membrane Potential ◽  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Electro Optic

Electro-optic response of second-harmonic generation membrane potential sensors

2003 ◽  
Vol 28 (8) ◽  
pp. 625 ◽  
Author(s):  
L. Moreaux ◽  
T. Pons ◽  
V. Dambrin ◽  
M. Blanchard-Desce ◽  
J. Mertz
Keyword(s):  
Membrane Potential ◽  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Electro Optic

scholarly journals Second-Harmonic Generation Imaging of Membrane Potential with Photon Counting

2008 ◽  
Vol 14 (6) ◽  
pp. 526-531 ◽  
Author(s):  
Jiang Jiang ◽  
Rafael Yuste
Keyword(s):  
Membrane Potential ◽  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Photon Counting ◽  
Analog Voltage ◽  
Second Harmonic Generation Imaging ◽  
Kinetics Of ◽  
Small Voltage ◽  
Pulse Variation

AbstractSecond-harmonic generation (SHG) can be used for imaging membrane potential in neurons, but poor signal-to-noise (S/N) limits accurate measurements of small voltage transients. We use photon counting to improve the S/N of weak SHG signal detection. Photon counting generates shot-noise limited and integrable signals, eliminates pulse-to-pulse variation, and built-in discriminators reduces the background to practically zero. In single trials, by using photon counting, we obtain a more than a twofold S/N increase over analog voltage detection. Trial-to-trial variability is also reduced by 50%. Finally, we show that, using photon counting, the kinetics of fast events such as action potentials can be recorded more accurately.

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