The Dura as Anti-Adhesive Coating Polypropylene Implant for Laparoscopic Hernioplasty

10.12737/5948 ◽  
2014 ◽  
Vol 8 (1) ◽  
pp. 1-5
Author(s):  
Воробейчук ◽  
G. Vorobeychuk ◽  
Драйер ◽  
M. Drayer ◽  
Тлибекова ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Wistar Rats ◽  
Research Work ◽  
Parietal Peritoneum ◽  
Grid Structure ◽  
Adhesive Properties ◽  
Internal Organs ◽  
Laparoscopic Hernioplasty ◽  
Adhesive Coating

This study presents the results of investigation of anti-adhesive properties of the dura combined with polypropylene implant, and a composite mash with a hydrogellayer Parietex Composite. 40 Wistar rats were used for this research work. The study showed that the use of the dura as an anti-adhesive coating prevents fusion of the internal organs with the implant, and the polypropylene used as a mash basis, firmly fuses with the parietal peritoneum, promoting in growth of connective tissue in the grid structure. These results are comparable to results obtained using Parietex Composite.

The Cuvierian Tubules of Holothuria leucospilota

1957 ◽  
Vol s3-98 (44) ◽  
pp. 455-472
Author(s):  
R. ENDEAN
Keyword(s):  
Connective Tissue ◽  
Outer Layer ◽  
Sea Water ◽  
Adhesive Properties ◽  
Chemical Constitution ◽  
Longitudinal Muscles ◽  
Testicular Hyaluronidase ◽  
Ground Mass

Each Cuvierian tubule of Holothuria leucospilota consists of a bounding layer of large cells beneath which are bundles of fibres embedded in a clear ground-mass. Present also is an axial cavity containing a core of morula-shaped cells. Immediately below the outer layer longitudinal muscles occur and, internal to these, muscle-bands which run spirally are found. The cells of the outer layer contain either granulated compartments or angular bodies. Both compartments and angular bodies contain proteinaceous materials. When a tubule is stretched in sea water the cells of the outer layer split and their contents form an amorphous mass which has strong adhesive properties. The fibres present in the connective tissue of the tubules are collagenous and are associated with acid polysaccharides part, at least, of which are mucopolysaccharides. A polysaccharide which can be removed by testicular hyaluronidase is present in the interfibrillar ground-mass. The chemical constitution of the tubule connective tissue is similar in many respects to that of vertebrate connective tissue. The mode of discharge and elongation of the tubules is discussed.

scholarly journals Study of the Acute Toxicity of a New Dosage Form of Naloxone Hydrochloride for Intranasal Administration

Drug Research ◽  
2019 ◽  
Vol 70 (01) ◽  
pp. 23-25
Author(s):  
Eduard A. Bariev ◽  
Ivan I. Krasnyuk ◽  
Maria N. Anurova ◽  
Elena O. Bakhrushina ◽  
Valery V. Smirnov ◽  
...  
Keyword(s):  
Body Weight ◽  
Protein Metabolism ◽  
Intranasal Administration ◽  
Wistar Rats ◽  
Relative Mass ◽  
General State ◽  
Initial Body Weight ◽  
Internal Organs ◽  
Naloxone Hydrochloride ◽  
Observation Period

AbstractThe experiment was conducted on 10 Wistar rats, male and female, with initial body weight 270–280 g (males) and 250–260 g (females). The drug was administered using a spray cap in 10 doses of 0.1 mg at 45 min intervals. The average cumulative dose of the drug per naloxone hydrochloride was 36.6 mg/kg for males and 39.4 mg/kg for females. The animals were monitored for 2 weeks after the exposure and then euthanized by a gentle decapitation.We noticed that after each drug administration the animals showed a decrease in motor activity. During the observation period there were no animal deaths or signs of abnormalities in their general state or behavior. Beginning on day 7 a significant increase in body weight of the animals was noted in comparison with the initial data. The relative mass of the internal organs of the treated rats remained within the physiological norm.We conclude that naloxone hydrochloride after an intranasal administration at 36.6 mg/kg for males and 39.4 mg/kg for females does not cause death of animals and or have a toxic effect on their general state, does not change their protein metabolism characteristics or the appearance of the internal organs and their mass.

scholarly journals A Systematic Approach to the Application of Soft Tissue Histopathology in Paleopathology

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Christina Grove ◽  
Oliver Peschel ◽  
Andreas G. Nerlich
Keyword(s):  
Soft Tissue ◽  
Connective Tissue ◽  
Time Interval ◽  
Tissue Preservation ◽  
Fat Tissue ◽  
Short Term ◽  
Internal Organs ◽  
Time Intervals ◽  
Pathological Conditions ◽  
Desert Areas

The application of histology to soft tissue remains offers an important technique to obtain diagnostically important information on various physiological and pathological conditions in paleopathology. In a series of 29 cases with mummified tissue ranging between 16 months and c. 5.200 years of postmortem time interval, we systematically investigated paleohistology and the preservation of various tissues. We established a reproducible histological ranking system for the evaluation of mummified tissue preservation. The application of this scheme to the series showed good tissue preservation of tissues with high connective tissue content but also fat tissue and connective tissue rich organs, such as lung tissue, while most other internal organs were less well preserved despite highly different postmortem time intervals. There are some organs with only poor conservation even in short term periods such as the kidneys and CNS. Artificial mummification does not provide better conservation than naturally mummified tissues; “cold” mummies may be much better conserved than those from desert areas. The identification of specific pathologies underlines the potential power of paleohistology.

scholarly journals Association of Immunofluorescence pattern of Antinuclear Antibody with Specific Autoantibodies in the Bangladeshi Population

2015 ◽  
Vol 40 (2) ◽  
pp. 74-78 ◽  
Author(s):  
S Sharmin ◽  
S Ahmed ◽  
A Abu Saleh ◽  
F Rahman ◽  
MR Choudhury ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Lupus Erythematosus ◽  
Antinuclear Antibody ◽  
Research Work ◽  
Connective Tissue Disorder ◽  
Nuclear Antigen ◽  
Dot Blot ◽  
Serum Samples ◽  
Speckled Pattern ◽  
Extractable Nuclear Antigen

Antinuclear antibody (ANA) is useful in the diagnosis of connective tissue disorder (CTD). Association of specific autoantibodies with the immunofluorescence pattern of ANA in CTD, noted in western literature has been considered as reference in all over the world. However, in Bangladesh no such research work or data correlating the autoantibodies and their ANA patterns is found. Objective of the study was to identify an association between immunofluorescence patterns of antinuclear antibody on HEp-2 cell and more specific antinuclear reactivities (e.g. anti-dsDNA and anti-extractable nuclear antigen) in the serum samples of CTD patients. Serum samples of 152 CTD patients (Systemic lupus erythematosus, Rhumatoid arthritis, Sjogren´s syndrome, Systemic sclerosis, Polymyositis, Mixed connective tissue disease) were diagnosed clinically, attending at Bangabandhu Sheikh Mujib Medical University (BSMMU) during the study period of January, 2010 to December, 2010. Samples were subjected for ANA testing by Indirect Immunofluorescence (IIF) on HEp-2 cell (ALPHADIA) in dilution of 1:40, anti-dsDNA by ELISA and anti- extractable nuclear antigen (anti-ENA) by Dot Immunoblot. Dot blot strips were tested for anti-Sm, anti-RNP, anti-SSA/Ro, anti-SSB/La, anti-Scl-70 and anti-Jo-1. Out of 152 patients 110 (72.3%) cases were ANA positive by IIF on HEp-2 cell. ANA positive sera exhibited four fluorescence patterns such as speckled (50.8%), peripheral (21.6%) ,homogenous (18.1%) and nucleolar pattern (9%). Peripheral pattern and homogenous pattern was predominantly associated with anti-dsDNA (p<0.05). Speckled pattern was significantly associated with anti-ENA (p<0.05).The most commonly identified antinuclear autoreactivity was directed towards anti-RNP (25.7%) then anti-Scl-70 (20%), anti-SSA (14.2%) and anti-SSB (5.7%). Multiple anti-ENA reactivities were identified in 34.28% cases. Peripheral and homogenous pattern is strongly associated with anti-dsDNA and speckled pattern may predict anti-ENA (specially ribonucleoprotiens). As a definite correlation between the ANA patterns and the group of antibodies was detected by dot immunoblot, one could predict presence of certain specific auto antibodies for a particular ANA pattern identified. This may restrict on the cost of laboratory investigations in a developing country like Bangladesh. Thus, ANA-IIF method may reduce the expense of detailed immunological work-up with minimal loss in diagnostic accuracy.Bangladesh Med Res Counc Bull 2014; 40 (2): 74-78

scholarly journals A Comparative Study of Biocompatibility in Rat Connective Tissue of a New Mineral Trioxide Compound (Theracal) versus MTA and a Bioactive G3 Glass

2021 ◽  
Vol 10 (12) ◽  
pp. 2536
Author(s):  
Jesús Mena-Álvarez ◽  
Cristina Rico-Romano ◽  
Carlos Gutiérrez-Ortega ◽  
Pablo Arias-Sanz ◽  
Javier Castro-Urda
Keyword(s):  
Connective Tissue ◽  
Inflammatory Reaction ◽  
Direct Contact ◽  
Wistar Rats ◽  
Tissue Response ◽  
New Mineral ◽  
Exact Test ◽  
Dorsal Area ◽  
Subcutaneous Connective Tissue ◽  
Hematoxylin Eosin

The aim of this paper was to assess the differences in tissue response to implantation during 15, 30 and 45 days in the subcutaneous connective tissue of Wistar rats from three biomaterials: Angelus MTA®, Theracal LC®, and Angelus MTA® to which 25% bioglass G3 was added. Twenty-four Wistar rats were used, the materials were inserted into the rat’s dorsal area in silicone tubes 5 mm long by 1.5 mm diameter. Histological reaction was assessed at 15, 30, and 45 days after implantation. They were then stained with hematoxylin eosin and evaluated by two observers. Data were analyzed using Fisher’s exact test and Mann–Whitney’s U test was used to determine the association between variables. Angelus MTA induced the formation of dystrophic calcifications twice as much as Theracal LC (p < 0.05). The addition of G3 did not affect the greater or lesser occurrence of calcifications (p > 0.05). Theracal LC and MTA plus G3 caused an inflammatory reaction, which was chronic at 15 days and decreased in intensity, almost disappearing after 45 days. Theracal LC, as well as Angelus MTA plus G3, were well tolerated when implanted in the subcutaneous connective tissue of rat. Histologically, no inconvenience was found for the use by direct contact of Theracal LC, and the mixture of MTA with 25% bioactive glass G3, in the tissue of Wistar rats.

scholarly journals Slaughter indicators of pigs when feeding cellulose-amylolytic additives

2019 ◽  
pp. 108-113
Author(s):  
V. Y. Novakovska
Keyword(s):  
Physiological State ◽  
Research Work ◽  
Internal Organs ◽  
Slaughter Weight ◽  
Fattening Pigs

The analysis of the research work on the study of slaughter indicators of fattening pigs when feeding cellulose-amylolytic additive in the composition of the diet is presented. The indicators of pre-slaughter weight, slaughter weight, slaughter output and weight of the internal organs are evaluated. It has been found that the use of cellulose-amylolytic additive in the amount of 19.2 g per day in the diet affects the physiological state of animals.

scholarly journals Histological analysis of state of tissues in internal organs of black-backed shad Alosa kessleri kessleri

2017 ◽  
Vol 188 (1) ◽  
pp. 155-161
Author(s):  
Maria P. Grushko ◽  
Nadezhda N. Fedorova ◽  
Alina A. Aitimova
Keyword(s):  
Connective Tissue ◽  
Environmental Pollution ◽  
Histological Analysis ◽  
Internal Organs ◽  
Common Features ◽  
Tissue Proliferation ◽  
Wide Range ◽  
Negative Impacts

Internal organs of black-backed shad are examined by histological analysis. Wide range of pathologies is found, which are supposedly caused by chronic environmental pollution. The pathologies of tissue and cellular levels have common features but various symptoms, as edema, hemorrhage, necrosis, inflammation, connective tissue proliferation, proliferative changes in the epithelium. The gills, liver; kidney, and intestines are the most sensitive to negative impacts of environments.

scholarly journals Possible Antioxidant and Haematinic Properties of the Stem Bark of Theobroma cacao L. in Wistar Rats

2021 ◽  
pp. 18-26
Author(s):  
B. O. Oluwatayo ◽  
T. A. Kolawole ◽  
C. C. Wali ◽  
O. A. Olayanju ◽  
A. E. J. Okwori
Keyword(s):  
Aqueous Extract ◽  
Theobroma Cacao ◽  
Wistar Rats ◽  
Antioxidant Activities ◽  
Research Work ◽  
Stem Bark ◽  
Dependent Manner ◽  
Group 2 ◽  
Dose Dependent ◽  
Theobroma Cacao L

Background: This study investigated the potential antioxidant effects of aqueous extract of the stem bark of Theobroma cacao L. in Wistar rats. Methods: Twenty Wistar rats weighing between 126 g – 224 g were grouped randomly into 4groups of 5 rats each. Group 1 served as control and received water while groups 2, 3 and 4 rats were given 1000mg/kg, 3000mg/kg and 5000mg/kg b.wt of the extract respectively for 28days. On the 29th day, the rats were anaesthetized and blood samples were collected for analysis of some haematological parameters, enzymatic and non- enzymatic antioxidant activities. Results: The results obtained showed that there was significant increase (p<0.001) in SOD, Catalase activities and MDA levels in a dose dependent manner. The results also showed significant increase (p<0.001) in RBC Group 2, 3 and 4 rats when compared to the Group1. Significant increase was also observed in Hemoglobin (Hb) and Hematocrit (Hct) level in group 2 and 3 rats (p<0.001). Mean corpuscular volume was significantly increased in group 2 rats (p<0.001). Conclusion: The findings from this study showed the antioxidant and hematinic potentials of the stem bark of Theobroma cacao L.The aqueous extract of the stem bark of Theobroma cacao L. has a potential antioxidative and hematinic effects in Wistar rats. This is largely due to its rich phytochemical and nutritive contents. Further research work will be needed to see the possible application of these properties in humans.

scholarly journals Broad-Spectrum Anti-Adhesive Coating Based on an Extracellular Polymer from a Marine Cyanobacterium

Marine Drugs ◽  
2019 ◽  
Vol 17 (4) ◽  
pp. 243 ◽  
Author(s):  
Bruna Costa ◽  
Rita Mota ◽  
Paula Parreira ◽  
Paula Tamagnini ◽  
M. Cristina L. Martins ◽  
...  
Keyword(s):  
Medical Device ◽  
Plasma Proteins ◽  
Bacterial Resistance ◽  
Thrombus Formation ◽  
Adhesive Properties ◽  
Marine Cyanobacterium ◽  
Technological Platform ◽  
Etiological Agents ◽  
Extracellular Polymer ◽  
Adhesive Coating

Medical device-associated infections are a major health threat, representing about half of all hospital-acquired infections. Current strategies to prevent this problem based on device coatings with antimicrobial compounds (antibiotics or antiseptics) have proven to be insufficient, often toxic, and even promoting bacterial resistance. Herein, we report the development of an infection-preventive coating (CyanoCoating) produced with an extracellular polymer released by the marine cyanobacterium Cyanothece sp. CCY 0110. CyanoCoating was prepared by spin-coating and its bacterial anti-adhesive efficiency was evaluated against relevant etiological agents (Staphylococcus aureus, S. epidermidis, Pseudomonas aeruginosa and Escherichia coli) and platelets, both in the presence or absence of human plasma proteins. CyanoCoating cytotoxicity was assessed using the L929 fibroblasts cell line. CyanoCoating exhibited a smooth topography, low thickness and high hydrophilic properties with mild negative charge. The non-cytotoxic CyanoCoating prevented adhesion of all the bacteria tested (≤80%) and platelets (<87%), without inducing platelet activation (even in the presence of plasma proteins). The significant reduction in protein adsorption (<77%) confirmed its anti-adhesive properties. The development of this anti-adhesive coating is an important step towards the establishment of a new technological platform capable of preventing medical device-associated infections, without inducing thrombus formation in blood-contacting applications.

Sign in / Sign up

Export Citation Format

Share Document