scholarly journals Subcellular distribution of 5-hydroxytryptamine in rabbit brain stem

1970 ◽  
Vol 66 (4) ◽  
pp. 400-407 ◽  
Author(s):  
Tomio SEGAWA ◽  
Isami KURUMA ◽  
Katsuya TAKATSUKA ◽  
Hiroshi TAKAGI
Keyword(s):  
Brain Stem ◽  
Subcellular Distribution ◽  
Rabbit Brain

The raphe nuclei of the rabbit brain stem

1979 ◽  
Vol 187 (1) ◽  
pp. 199-243 ◽  
Author(s):  
David L. Felten ◽  
John P. Cummings
Keyword(s):  
Brain Stem ◽  
Raphe Nuclei ◽  
Rabbit Brain ◽  
Raphé Nuclei

scholarly journals Resting and stimulated respiration in vitro in the central nervous system. Regional distribution and relation to cell density

1967 ◽  
Vol 105 (2) ◽  
pp. 831-835 ◽  
Author(s):  
J. W. Ridge
Keyword(s):  
Cerebral Cortex ◽  
Respiratory Rate ◽  
Brain Stem ◽  
Cell Density ◽  
Regional Distribution ◽  
Dry Weight ◽  
Weight Basis ◽  
Rabbit Brain ◽  
Percentage Increase

1. The respiratory rates in vitro of ten structures of the rabbit brain were measured in the presence of a normal (5mm) and an elevated (50mm) concentration of K+ ions. The results were expressed on a dry-weight basis and in terms of cell density. 2. On a dry-weight basis, with a normal concentration of K+, there was a steady decrease in respiratory rate on passing from the cerebral cortex through successively lower centres to the brain-stem, i.e. respiration was negatively correlated with phylogenetic age. 3. When the resting respiratory rates were expressed in terms of cell density there was no correlation with phylogenetic age. The neuron-containing structures of the cerebrum and brain-stem had identical respiratory rates with the exception of the cerebral cortex which had a higher rate. 4. The K+-stimulated respiratory rates/cell also showed a negative correlation with phylogenetic age; the higher centres responded with a greater percentage increase than the lower. 5. The results from the cerebellar cortex were anomalous. 6. There were variations of respiratory rate within the diencephalon. The thalamus had a relatively high respiratory rate, the anterior and ventral regions relatively low and the hypothalamus intermediate. 7. The oxygen consumption of white matter was not increased by a high external concentration of K+.

The influences of drugs on the uptake of 5-hydroxytryptamine by synaptic vesicles of rabbit brain stem

1968 ◽  
Vol 20 (10) ◽  
pp. 800-801 ◽  
Author(s):  
Tomio Segawa ◽  
Isami Kuruma ◽  
Katsuya Takatsuka ◽  
Hiroshi Takagi
Keyword(s):  
Brain Stem ◽  
Synaptic Vesicles ◽  
Rabbit Brain

scholarly journals Biochemical properties and subcellular distribution of the BI and rbA isoforms of alpha 1A subunits of brain calcium channels.

1996 ◽  
Vol 134 (2) ◽  
pp. 511-528 ◽  
Author(s):  
T Sakurai ◽  
R E Westenbroek ◽  
J Rettig ◽  
J Hell ◽  
W A Catterall
Keyword(s):  
Calcium Channels ◽  
Subcellular Distribution ◽  
Mossy Fiber ◽  
Biochemical Properties ◽  
Rabbit Brain ◽  
Nerve Terminals ◽  
Brain Membrane ◽  
Receptor Sites ◽  
Molecular Masses ◽  
Affinity Receptor

Biochemical properties and subcellular distribution of the class A calcium channel alpha 1 subunits (alpha 1A) from rat and rabbit brain were examined using site-directed anti-peptide antibodies specific for rat rbA (anti-CNA3) and for rabbit BI (anti-NBI-1 and anti-NBI-2) isoforms of alpha 1A. In immunoblotting experiments, anti-CNA3 specifically identifies multiple alpha 1A polypeptides with apparent molecular masses of 210, 190, and 160 kD, and anti-NBI-1 and anti-NBI-2 specifically recognize 190-kD alpha 1A polypeptides in rat brain membrane. In rabbit brain, anti-NBI-1 or anti-NBI-2 specifically detect alpha 1A polypeptides with apparent molecular masses of 220, 200, and 190 kD, while anti-CNA3 specifically recognizes 190-kD alpha 1A polypeptides. These polypeptides evidently represent multiple isoforms of alpha 1A present in both rat and rabbit brain. Anti-CNA3 specifically immunoprecipitates high affinity receptor sites for omega-conotoxin MVIIC (Kd approximately 100 pM), whereas anti-NBI-2 immunoprecipitates two distinct affinity receptor sites for omega-conotoxin MVIIC (Kd approximately 100 pM and approximately 1 microM). Coimmunoprecipitation experiments indicate that alpha 1A subunits recognized by anti-CNA3 and anti-NBI-2 are associated with syntaxin in a stable, SDS-resistant complex and with synaptotagmin. Immunofluorescence studies reveal that calcium channels recognized by anti-NBI-2 are localized predominantly in dendrites and nerve terminals forming synapses on them, while calcium channels recognized by anti-CNA3 are localized more prominently in cell bodies and in nerve terminals. The mossy fiber terminals in hippocampus and the terminals of climbing and parallel fibers in cerebellum are differentially stained by these isoform-specific antibodies. These results indicate that both rbA and BI isoforms of alpha 1A are expressed in rat and rabbit brain and form calcium channels having alpha 1A subunits with distinct molecular mass, pharmacology, and subcellular localization.

scholarly journals UPTAKE AND STORAGE MECHANISM OF 5-HYDRO-XYTRYPTAMINE IN RABBIT BRAIN STEM AND EFFECT OF RESERPINE

1971 ◽  
Vol 21 (1) ◽  
pp. 57-67 ◽  
Author(s):  
Katsuya TAKATSUKA ◽  
Tomio SEGAWA ◽  
Hiroshi TAKAGI
Keyword(s):  
Brain Stem ◽  
Rabbit Brain ◽  
Storage Mechanism ◽  
And Storage
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