scholarly journals Non-random chromosome positioning in mammalian sperm nuclei, with migration of the sex chromosomes during late spermatogenesis

2005 ◽  
Vol 118 (9) ◽  
pp. 1811-1820 ◽  
Author(s):  
H. A. Foster
Keyword(s):  
Sex Chromosomes ◽  
Mammalian Sperm ◽  
Sperm Nuclei

scholarly journals Interspecies Differences in the Stability of Mammalian Sperm Nuclei Assessed in Vivo by Sperm Microinjection and in Vitro by Flow Cytometry1

1988 ◽  
Vol 39 (1) ◽  
pp. 157-167 ◽  
Author(s):  
Sally D. Perreault ◽  
Randy R. Barbee ◽  
Kenneth H. Elstein ◽  
Robert M. Zucker ◽  
Carol L. Keefer
Keyword(s):  
Interspecies Differences ◽  
Mammalian Sperm ◽  
Sperm Nuclei ◽  
The Stability

The centromere specific histone CENP-A is selectively retained in discrete foci in mammalian sperm nuclei

Chromosoma ◽  
1990 ◽  
Vol 100 (1) ◽  
pp. 32-36 ◽  
Author(s):  
Douglas K. Palmer ◽  
Kathleen O'Day ◽  
Robert L. Margolis
Keyword(s):  
Mammalian Sperm ◽  
Sperm Nuclei

Segregation of sex chromosomes into sperm nuclei in a man with 47,XXY Klinefelter's karyotype: a FISH analysis

1997 ◽  
Vol 99 (4) ◽  
pp. 474-477 ◽  
Author(s):  
M. Guttenbach ◽  
H. W. Michelmann ◽  
B. Hinney ◽  
W. Engel ◽  
M. Schmid
Keyword(s):  
Sex Chromosomes ◽  
Fish Analysis ◽  
Sperm Nuclei

Zinc is sufficiently abundant within mammalian sperm nuclei to bind stoichiometrically with protamine 2

2000 ◽  
Vol 56 (4) ◽  
pp. 512-519 ◽  
Author(s):  
G. Bench ◽  
M.H. Corzett ◽  
C.E. Kramer ◽  
P.G. Grant ◽  
R. Balhorn
Keyword(s):  
Mammalian Sperm ◽  
Sperm Nuclei ◽  
Protamine 2

scholarly journals Flow cytometry of mammalian sperm: progress in DNA and morphology measurement.

1979 ◽  
Vol 27 (1) ◽  
pp. 353-358 ◽  
Author(s):  
D Pinkel ◽  
P Dean ◽  
S Lake ◽  
D Peters ◽  
M Mendelsohn ◽  
...  
Keyword(s):  
Mammalian Species ◽  
Human Sperm ◽  
High Refractive Index ◽  
Optical Effect ◽  
Related Information ◽  
Mammalian Sperm ◽  
Flow Cytometric ◽  
Dual Beam ◽  
Sperm Nuclei ◽  
Dna Measurements

Variability in DNA content and head shape of mammalian sperm are potentially useful markers for flow cytometric monitoring of genetic damage in spermatogenic cells. The high refractive index and extreme flatness of the sperm heads produce an optical effect which interferes with DNA measurements in flow cytometers which have dye excitation and fluorescence light collection normal to the axis of flow. Orientation of sperm in flow controls this effect and results in coefficients of variation of 2.5% and 4.2%, respectively, for DNA measurements of mouse and human sperm. Alternatively, the optical effect can be used to generate shape-related information. Measurements on randomly oriented sperm from three mammalian species using a pair of fluorescence detectors indicate that large shape differences are detectable. Acriflavine-Feulgen stained sperm nuclei are significantly bleached during flow cytometric measurements at power levels routinely used in many flow cytometers. Dual beam studies of this phenomenon indicate it may be useful in detecting abnormally shaped sperm.

Meiotic behaviour of sex chromosomes investigated by three-colour FISH on 35 142 sperm nuclei from two 47,XYY males

1997 ◽  
Vol 99 (3) ◽  
pp. 407-412 ◽  
Author(s):  
E. Chevret ◽  
S. Rousseaux ◽  
M. Monteil ◽  
Y. Usson ◽  
J. Cozzi ◽  
...  
Keyword(s):  
Sex Chromosomes ◽  
Meiotic Behaviour ◽  
Sperm Nuclei

Development of mammalian sperm nuclei in an amphibian cell-free system

1994 ◽  
Vol 41 (1) ◽  
pp. 318
Author(s):  
V. Tok ◽  
S.C. Ng ◽  
M. Montag ◽  
S.L. Liow ◽  
A. Bongso ◽  
...  
Keyword(s):  
Free System ◽  
Cell Free System ◽  
Mammalian Sperm ◽  
Sperm Nuclei

Past, present and future perspectives on sexing sperm

2003 ◽  
Vol 83 (3) ◽  
pp. 375-384 ◽  
Author(s):  
D. L. Garner ◽  
G. E. Seidel Jr.
Keyword(s):  
Flow Cytometry ◽  
Key Words ◽  
Sex Chromosomes ◽  
Dna Content ◽  
Sex Chromosome ◽  
Future Perspectives ◽  
Hoechst 33342 ◽  
Cell Sorter ◽  
Mammalian Sperm ◽  
Bovine Sperm

Development of flow cytometry for sorting mammalian sperm according to their sex chromosomes began in the late 1970s and early 1980s. This technology, which has recently been commercialized for bovine sperm, is based on the differences in DNA content between X- and Y-chromosome-bearing sperm. Under ideal conditions, 5000 live bovine sperm of each sex can be sorted per second at 90% accuracy. Pregnancy rates of 50% have been achieved routinely in well-managed heifers with sex-sorted, cryopreserved bovine sperm compared to 60–80% with unsexed control sperm. About 90% of offspring have been of the selected sex. Sorting sperm according to sex chromosome content is similarly successful in many other mammals including exotic species, but sorting efficiencies are somewhat less for sperm from some species. Key words: Mammals, sex chromosomes, flow cytometer, cell sorter, DNA content, X and Y sperm, Hoechst 33342

Synthesis and amino acid composition of basic proteins in mammalian sperm nuclei

1975 ◽  
Vol 47 (2) ◽  
pp. 349-365 ◽  
Author(s):  
Anthony R. Bellvé ◽  
Everett Anderson ◽  
Linda Hanley-Bowdoin
Keyword(s):  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Basic Proteins ◽  
Mammalian Sperm ◽  
Sperm Nuclei
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