Evaluation of segregation patterns of 21;21 Robertsonian translocation along with sex chromosomes and interchromosomal effects in sperm nuclei of carrier by FISH technique

2002 ◽  
Vol 63 (2) ◽  
pp. 232-236 ◽  
Author(s):  
Hasan Acar ◽  
M. Selman Yildirim ◽  
Tülin Çora ◽  
Serdar Ceylaner
Keyword(s):  
Sex Chromosomes ◽  
Robertsonian Translocation ◽  
Fish Technique ◽  
Sperm Nuclei

scholarly journals Application of the FISH Technique to Visualize Sex Chromosomes in Domestic Cat Spermatozoa

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 2106
Author(s):  
Barbara Kij-Mitka ◽  
Halina Cernohorska ◽  
Svatava Kubickova ◽  
Sylwia Prochowska ◽  
Wojciech Niżański ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Sex Chromosomes ◽  
Chromosomal Abnormalities ◽  
Molecular Cytogenetics ◽  
Molecular Probes ◽  
Domestic Cat ◽  
Fish Technique ◽  
Y Chromosomes

Fluorescence in situ hybridization is a molecular cytogenetics technique that enables the visualization of chromosomes in cells via fluorescently labeled molecular probes specific to selected chromosomes. Despite difficulties in carrying out the FISH technique on sperm, related to the need for proper nuclear chromatin decondensation, this technique has already been used to visualize chromosomes in human, mouse, cattle, swine, horse, and dog spermatozoa. Until now, FISH has not been performed on domestic cat sperm; therefore, the aim of this study was to visualize sex chromosomes in domestic cat sperm. The results showed the presence of X and Y chromosomes in feline spermatozoa. The procedure used for sperm decondensation and fluorescence in situ hybridization was adequate to visualize chromosomes in domestic cat spermatozoa and, in the future, it may be used to determine the degree of chromosomal abnormalities in these gametes.

scholarly journals Cytogenetics of the neotropical flesh fly Pattonela intermutans (Diptera, Sarcophagidae)

2000 ◽  
Vol 23 (3) ◽  
pp. 563-567 ◽  
Author(s):  
Patricia Pasquali Parise-Maltempi ◽  
Rita Maria Pereira Avancini
Keyword(s):  
Sex Chromosomes ◽  
Chromosome Pair ◽  
Sex Chromosome ◽  
Organizer Region ◽  
Nucleolar Organizer Region ◽  
Nucleolar Organizer ◽  
Pericentromeric Region ◽  
Flesh Fly ◽  
Fish Technique

Pattonella intermutans has 2n = 12 chromosomes including three metacentric and two submetacentric pairs of autosomes and an XX/XY sex chromosome pair. The autosomes are characterized by the presence of a C band in the pericentromeric region while sex chromosomes are totally heterochromatic. The FISH technique showed a nucleolar organizer region (NOR) in autosome IV.

scholarly journals The influence of the Robertsonian translocation Rb(X.2)2Ad on anaphase I non-disjunction in male laboratory mice

1989 ◽  
Vol 53 (2) ◽  
pp. 77-86 ◽  
Author(s):  
I.-D. Adler ◽  
R. Johannisson ◽  
H. Winking
Keyword(s):  
Sex Chromosomes ◽  
Robertsonian Translocation ◽  
Sex Chromosome ◽  
Light And Electron Microscopy ◽  
Normal Karyotype ◽  
Chromosome 2 ◽  
Male Genome ◽  
The Difference ◽  
Normal Pairing

SummaryA Robertsonian translocation in the mouse between theXchromosome and chromosome 2 is described. The male and female carriers of the Rb(X.2)2Ad were fertile. A homozygous/hemizygous line was maintained. The influence of theX-autosomal Robertsonian translocation on anaphase I non-disjunction in male mice was studied by chromosome counts in cells at metaphase II of meoisis and by assessment of aneuploid progeny. The results conclusively show that the inclusion of Rb2Ad in the male genome induces non-disjunction at the first meoitic division. In second metaphase cells the frequency of sex-chromosomal aneuploidy was 10·8%, and secondary spermatocytes containing two or no sex chromosome were equally frequent. The Rb2Ad males sired 3·9% sex-chromosome aneuploid progeny. The difference in aneuploidy frequencies in the germ cells and among the progeny suggests that the viability of XO and XXY individuals is reduced. The pairing configurations of chromosomes 2, Rb2Ad andYwere studied during meiotic prophase by light and electron microscopy. Trivalent pairing was seen in all well spread nuclei. Complete pairing of the acrocentric autosome 2 with the corresponding segment of the Rb2Ad chromosome was only seen in 3·2% of the cells analysed in the electron microscope. The pairing between theXand theYchromosome in the Rb2Ad males corresponded to that in males with normal karyotype. Reasons for sex-chromosomal non-disjunction despite the normal pairing pattern between the sex chromosomes may be seen in the terminal chiasma location coupled with the asynchronous separation of the sex chromosomes and the autosomes. The Rb2Ad chromosome can be useful for studies ofXinactivation, as a marker for parental derivation of theXchromosome and for mapping loci byin situhybridization.

Segregation of sex chromosomes into sperm nuclei in a man with 47,XXY Klinefelter's karyotype: a FISH analysis

1997 ◽  
Vol 99 (4) ◽  
pp. 474-477 ◽  
Author(s):  
M. Guttenbach ◽  
H. W. Michelmann ◽  
B. Hinney ◽  
W. Engel ◽  
M. Schmid
Keyword(s):  
Sex Chromosomes ◽  
Fish Analysis ◽  
Sperm Nuclei

scholarly journals Molecular cytogenetics analysis with whole chromosome paint probes of sperm nuclei from a (13;15) Robertsonian translocation carrier

2005 ◽  
Vol 50 (7) ◽  
pp. 360-364 ◽  
Author(s):  
Nathalie Rives ◽  
Célia Ravel ◽  
Véronique Duchesne ◽  
Jean-Pierre Siffroi ◽  
Nathalie Mousset-Siméon ◽  
...  
Keyword(s):  
Robertsonian Translocation ◽  
Molecular Cytogenetics ◽  
Translocation Carrier ◽  
Chromosome Paint ◽  
Sperm Nuclei

Meiotic behaviour of sex chromosomes investigated by three-colour FISH on 35 142 sperm nuclei from two 47,XYY males

1997 ◽  
Vol 99 (3) ◽  
pp. 407-412 ◽  
Author(s):  
E. Chevret ◽  
S. Rousseaux ◽  
M. Monteil ◽  
Y. Usson ◽  
J. Cozzi ◽  
...  
Keyword(s):  
Sex Chromosomes ◽  
Meiotic Behaviour ◽  
Sperm Nuclei

scholarly journals The phenomenon of cell chimerism in goats

2012 ◽  
Vol 50 (No. 7) ◽  
pp. 311-314 ◽  
Author(s):  
T. Rychlik ◽  
A. Kozubska-Sobocinska ◽  
B. Rejduch ◽  
J. Sikora
Keyword(s):  
Sex Chromosomes ◽  
Blood Cells ◽  
Cytogenetic Analysis ◽  
Chromosome Painting ◽  
Sex Chromosome ◽  
Same Sex ◽  
Fish Technique ◽  
Opposite Sex ◽  
Painting Probes ◽  
Two Populations

Cell chimerism was diagnosed in goats with test reagents that identify erythrocyte antigens and with bovine probes that paint sex chromosomes. Same-sex and opposite-sex twins and their parents, representing the Fawn Improved breed, were used in the study. Ovine test reagents (anti-Aa, -Be, -Bi, -Bd, -Bb, -Ca, -R) were used to analyse the blood groups of twins. Cytogenetic analysis was based on FISH technique. Identical antigens and incomplete results of the reaction of blood cells with some immune sera showed that these animals had two populations of erythrocytes differing in antigens A<sub>1</sub>, B<sub>2</sub>, B<sub>3</sub>, B<sub>15 </sub>and R. The analysis of 100 metaphase plates for each animal, which were subjected to FISH technique using bovine sex chromosome painting probes, showed the presence of two cell lines: 60,XX and 60,XY.

Analysis of Meiotic Segregation Pattern and Interchromosomal Effects in a Bull Heterozygous for a 3/16 Robertsonian Translocation

2018 ◽  
Vol 156 (4) ◽  
pp. 197-203 ◽  
Author(s):  
Harmonie Barasc ◽  
Nathalie Mouney-Bonnet ◽  
Clémence Peigney ◽  
Anne Calgaro ◽  
Clémence Revel ◽  
...  
Keyword(s):  
Robertsonian Translocation ◽  
Chromosomal Rearrangements ◽  
Segregation Pattern ◽  
Fish Analysis ◽  
Meiotic Segregation ◽  
Robertsonian Translocations ◽  
Sperm Nuclei ◽  
Meiotic Segregation Pattern ◽  
First Time ◽  
Low Rate

Robertsonian translocations are the most frequent chromosomal rearrangements detected in cattle. Here, we report on the detection of a new Robertsonian translocation between chromosomes BTA3 and BTA16. This rob(3;16) was dicentric, suggesting that its occurrence was recent. FISH analysis of decondensed sperm nuclei revealed a relatively low rate of unbalanced gametes produced by adjacent segregation (5.87%). In addition, and for the first time in bovines, a significant interchromosomal effect (ICE) was detected for 2 different autosomes: BTA17 (global disomy + nullisomy rate of 9%) and BTA20 (1.8%). These results suggest that ICE should be taken into consideration when assessing the putative effect of Robertsonian translocations on reproduction.

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