Meiotic behaviour of sex chromosomes investigated by three-colour FISH on 35 142 sperm nuclei from two 47,XYY males

1997 ◽  
Vol 99 (3) ◽  
pp. 407-412 ◽  
Author(s):  
E. Chevret ◽  
S. Rousseaux ◽  
M. Monteil ◽  
Y. Usson ◽  
J. Cozzi ◽  
...  
Keyword(s):  
Sex Chromosomes ◽  
Meiotic Behaviour ◽  
Sperm Nuclei

Segregation of sex chromosomes into sperm nuclei in a man with 47,XXY Klinefelter's karyotype: a FISH analysis

1997 ◽  
Vol 99 (4) ◽  
pp. 474-477 ◽  
Author(s):  
M. Guttenbach ◽  
H. W. Michelmann ◽  
B. Hinney ◽  
W. Engel ◽  
M. Schmid
Keyword(s):  
Sex Chromosomes ◽  
Fish Analysis ◽  
Sperm Nuclei

Meiotic behaviour of sex chromosomes and polymeiosis in three species of insectivores

Genetica ◽  
1984 ◽  
Vol 65 (3) ◽  
pp. 187-192 ◽  
Author(s):  
R. Jlm�nez ◽  
M. Burgos ◽  
R. Diaz de la Guardia
Keyword(s):  
Sex Chromosomes ◽  
Meiotic Behaviour

A High Resolution Cytochemical Study of Nuclear ATPase in Human Spermatozoa

1975 ◽  
Vol 33 ◽  
pp. 594-595
Author(s):  
A. Sosa ◽  
L. Calzada
Keyword(s):  
High Resolution ◽  
Atpase Activity ◽  
Nuclear Membrane ◽  
Human Sperm ◽  
Sperm Nucleus ◽  
Human Spermatozoa ◽  
Cytochemical Study ◽  
Membrane Bound ◽  
Sperm Nuclei ◽  
Interchromatin Space

The dependence of nuclear metabolism on the function of the nuclear membrane is not well understood. Whether or not the function of the nuclear membrane is partial or totally responsible of the repressed template activity of human sperm nucleus has not at present been elucidated. One of the membrane-bound enzymatic activities which is concerned with the mechanisms whereby substances are thought to cross cell membranes is adenosintriphosphatase (ATPase). This prompted its characterization and distribution by high resolution photogrammetry on isolated human sperm nuclei. Isolated human spermatozoa nuclei were obtained as previously described. ATPase activity was demonstrated by the method of Wachstein and Meisel modified by Marchesi and Palade. ATPase activity was identified as dense and irregularly distributed granules confined to the internal leaflet of the nuclear membrane. Within the nucleus the appearance of the reaction product occurs as homogenous and dense precipitates in the interchromatin space.

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