The centromere specific histone CENP-A is selectively retained in discrete foci in mammalian sperm nuclei

Chromosoma ◽  
1990 ◽  
Vol 100 (1) ◽  
pp. 32-36 ◽  
Author(s):  
Douglas K. Palmer ◽  
Kathleen O'Day ◽  
Robert L. Margolis
Keyword(s):  
Mammalian Sperm ◽  
Sperm Nuclei

scholarly journals Interspecies Differences in the Stability of Mammalian Sperm Nuclei Assessed in Vivo by Sperm Microinjection and in Vitro by Flow Cytometry1

1988 ◽  
Vol 39 (1) ◽  
pp. 157-167 ◽  
Author(s):  
Sally D. Perreault ◽  
Randy R. Barbee ◽  
Kenneth H. Elstein ◽  
Robert M. Zucker ◽  
Carol L. Keefer
Keyword(s):  
Interspecies Differences ◽  
Mammalian Sperm ◽  
Sperm Nuclei ◽  
The Stability

Zinc is sufficiently abundant within mammalian sperm nuclei to bind stoichiometrically with protamine 2

2000 ◽  
Vol 56 (4) ◽  
pp. 512-519 ◽  
Author(s):  
G. Bench ◽  
M.H. Corzett ◽  
C.E. Kramer ◽  
P.G. Grant ◽  
R. Balhorn
Keyword(s):  
Mammalian Sperm ◽  
Sperm Nuclei ◽  
Protamine 2

scholarly journals Flow cytometry of mammalian sperm: progress in DNA and morphology measurement.

1979 ◽  
Vol 27 (1) ◽  
pp. 353-358 ◽  
Author(s):  
D Pinkel ◽  
P Dean ◽  
S Lake ◽  
D Peters ◽  
M Mendelsohn ◽  
...  
Keyword(s):  
Mammalian Species ◽  
Human Sperm ◽  
High Refractive Index ◽  
Optical Effect ◽  
Related Information ◽  
Mammalian Sperm ◽  
Flow Cytometric ◽  
Dual Beam ◽  
Sperm Nuclei ◽  
Dna Measurements

Variability in DNA content and head shape of mammalian sperm are potentially useful markers for flow cytometric monitoring of genetic damage in spermatogenic cells. The high refractive index and extreme flatness of the sperm heads produce an optical effect which interferes with DNA measurements in flow cytometers which have dye excitation and fluorescence light collection normal to the axis of flow. Orientation of sperm in flow controls this effect and results in coefficients of variation of 2.5% and 4.2%, respectively, for DNA measurements of mouse and human sperm. Alternatively, the optical effect can be used to generate shape-related information. Measurements on randomly oriented sperm from three mammalian species using a pair of fluorescence detectors indicate that large shape differences are detectable. Acriflavine-Feulgen stained sperm nuclei are significantly bleached during flow cytometric measurements at power levels routinely used in many flow cytometers. Dual beam studies of this phenomenon indicate it may be useful in detecting abnormally shaped sperm.

Development of mammalian sperm nuclei in an amphibian cell-free system

1994 ◽  
Vol 41 (1) ◽  
pp. 318
Author(s):  
V. Tok ◽  
S.C. Ng ◽  
M. Montag ◽  
S.L. Liow ◽  
A. Bongso ◽  
...  
Keyword(s):  
Free System ◽  
Cell Free System ◽  
Mammalian Sperm ◽  
Sperm Nuclei

Synthesis and amino acid composition of basic proteins in mammalian sperm nuclei

1975 ◽  
Vol 47 (2) ◽  
pp. 349-365 ◽  
Author(s):  
Anthony R. Bellvé ◽  
Everett Anderson ◽  
Linda Hanley-Bowdoin
Keyword(s):  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Basic Proteins ◽  
Mammalian Sperm ◽  
Sperm Nuclei

A High Resolution Cytochemical Study of Nuclear ATPase in Human Spermatozoa

1975 ◽  
Vol 33 ◽  
pp. 594-595
Author(s):  
A. Sosa ◽  
L. Calzada
Keyword(s):  
High Resolution ◽  
Atpase Activity ◽  
Nuclear Membrane ◽  
Human Sperm ◽  
Sperm Nucleus ◽  
Human Spermatozoa ◽  
Cytochemical Study ◽  
Membrane Bound ◽  
Sperm Nuclei ◽  
Interchromatin Space

The dependence of nuclear metabolism on the function of the nuclear membrane is not well understood. Whether or not the function of the nuclear membrane is partial or totally responsible of the repressed template activity of human sperm nucleus has not at present been elucidated. One of the membrane-bound enzymatic activities which is concerned with the mechanisms whereby substances are thought to cross cell membranes is adenosintriphosphatase (ATPase). This prompted its characterization and distribution by high resolution photogrammetry on isolated human sperm nuclei. Isolated human spermatozoa nuclei were obtained as previously described. ATPase activity was demonstrated by the method of Wachstein and Meisel modified by Marchesi and Palade. ATPase activity was identified as dense and irregularly distributed granules confined to the internal leaflet of the nuclear membrane. Within the nucleus the appearance of the reaction product occurs as homogenous and dense precipitates in the interchromatin space.

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