Reduced Adjuvant Activity of Bordetella pertussis on the Secondary Immune Response of Mice Evoked by an Immunogenic Threshold Dose of Sheep Erythrocytes

Pathobiology ◽  
1969 ◽  
Vol 34 (6) ◽  
pp. 340-351
Author(s):  
H. Finger ◽  
P. Emmerling ◽  
G. Köthe
Keyword(s):  
Immune Response ◽  
Bordetella Pertussis ◽  
Threshold Dose ◽  
Secondary Immune Response ◽  
Adjuvant Activity ◽  
Sheep Erythrocytes

Antigen-sensitive spleen cells and the adjuvant activity of Bordetella pertussis

1970 ◽  
Vol 16 (7) ◽  
pp. 623-627 ◽  
Author(s):  
H. Finger ◽  
P. Emmerling ◽  
M. Büsse
Keyword(s):  
Immune Response ◽  
Bordetella Pertussis ◽  
Primary Immune Response ◽  
Target Cells ◽  
Primary Immunization ◽  
Spleen Cells ◽  
Adjuvant Effect ◽  
Adjuvant Activity ◽  
Simultaneous Injection ◽  
Sheep Erythrocytes

In this study we determined at both the cellular and humoral level whether or not the primary immune response of mice can be significantly enhanced by administration of a bacterial adjuvant after the primary immunization with sheep erythrocytes. As compared to the immunization of mice with 8 × 106 sheep erythrocytes alone, the simultaneous injection of 3 × 109Bordetella pertussis cells and 8 × 106 sheep erythrocytes resulted in an accelerated and prolonged multiplication of hemolysin-forming spleen cells. The adjuvant effect was also documented by increased production of serum hemolysins. When the bacterial adjuvant was given 6, 12, or 24 h after the primary antigenic stimulus, however, neither increased plaque counts nor enhanced serum hemolysin titers were detectable. These findings agree with the concept that B. pertussis cells cause multiplication of antigen-sensitive target cells or affect the initial stages of differentiation of these cells.

The influence of Bordetella pertussis on the preparation of mouse spleens for the secondary immune response

1969 ◽  
Vol 15 (7) ◽  
pp. 814-816 ◽  
Author(s):  
H. Finger ◽  
P. Emmering ◽  
E. Brüss
Keyword(s):  
Immune Response ◽  
Bordetella Pertussis ◽  
Lymphoid Tissues ◽  
Secondary Immune Response ◽  
Spleen Cells ◽  
Simultaneous Injection ◽  
Sheep Erythrocytes ◽  
Initial Injection ◽  
Erythrocyte Antigen

The simultaneous injection of 4 × 108 sheep erythrocytes and 3 × 109 killed cells of Bordetella pertussis effects an accelerated and prolonged multiplication of antibody-forming spleen cells if compared with mice immunized with the erythrocyte antigen only. If mice of both groups are given a second injection of 4 × 108 sheep erythrocytes 6 weeks later, the numbers of indirect plaque-forming cells are markedly increased in pertussis-treated mice. This is connected with enhanced 7 S serum hemolysin titers. The conclusion is drawn that an initial injection of B. pertussis prepares the lymphoid tissues of mice for the secondary immune response.

Variable Adjuvant Activity of Bordetella pertussis with Respect to the Primary and Secondary Immunization of Mice

1970 ◽  
Vol 1 (3) ◽  
pp. 251-258
Author(s):  
H. Finger ◽  
P. Emmerling ◽  
E. Brüss
Keyword(s):  
Immune Responses ◽  
Bordetella Pertussis ◽  
Serum Antibody ◽  
Secondary Response ◽  
Primary Immunization ◽  
Spleen Cells ◽  
Adjuvant Effect ◽  
Adjuvant Activity ◽  
Sheep Erythrocytes ◽  
X Cells

We carried out a study on the adjuvant effect of Bordetella pertussis vaccine on the primary and secondary immune responses of the mouse to sheep erythrocytes, quantitating antibody-producing spleen cells and serum antibody. The simultaneous injection of sheep erythrocytes and B. pertussis , when compared to immunization with sheep red blood cells alone, resulted in an increased and prolonged multiplication of antibody-forming spleen cells. The adjuvant effect was also documented by increased production of serum hemolysins and agglutinins. Further, B. pertussis enhanced the priming effect of the antigen for the secondary response. However, when the bacterial adjuvant was given together with a second antigenic stimulus 41 days after the primary immunization, the peak values of direct and indirect plaque-forming spleen cells did not differ from the corresponding control animals further inoculated with sheep erythrocytes alone. Nonetheless, the influence of the bacterial adjuvant was still expressed by the delayed decrease of the numbers of plaque-forming spleen cells. On the basis of the X-Y-Z scheme it is suggested that B. pertussis cells as adjuvant enhance the multiplication of antigen-sensitive X cells or affect the initial stages of differentiation of these cells. This effect of the pertussis vaccine can be distinguished from a general proliferative action on other cells.

Effect of route of immunization on development of antibody-forming cells in hilar lymph nodes

1980 ◽  
Vol 30 (2) ◽  
pp. 391-396
Author(s):  
J Stein-Streilein ◽  
D A Hart
Keyword(s):  
Immune Response ◽  
Respiratory Tract ◽  
Lymph Nodes ◽  
Lower Respiratory Tract ◽  
Immunoglobulin M ◽  
Secondary Immune Response ◽  
Sheep Erythrocytes ◽  
Low Concentrations ◽  
Draining Lymph Nodes ◽  
Intraperitoneal Inoculation

Studies were designed to compare the effect of the route of inoculation of sheep erythrocytes (SRBC) on the development of antibody-forming cells (AFC) in the hilar lymph nodes of BDF1 mice. The major observation from these studies was that the immunogenicity of low concentrations of antigen was dependent on the route of inoculation. Intratracheal, intravenous, or footpad inoculation of low numbers of SRBC (10(7)) did not stimulate development of AFC in hilar lymph nodes. However, intraperitoneal inoculation of the same concentration of antigen induced an immunoglobulin M AFC response in the hilar lymph nodes. Moreover, both intraperitoneal and intratracheal inoculation of 10(7) SRBC primed the mice for an anamnestic AFC response to a subsequent intratracheal inoculation of 10(7) SRBC. These findings support the hypothesis that presentation of antigen by the local or systemic routes may induce either or both a primary a secondary immune response in the draining lymph nodes of the lower respiratory tract.

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