Effect of distamycin a on the primary and secondary immune response of mouse spleen cellsin vitro against sheep erythrocytes

1975 ◽  
Vol 5 (1) ◽  
Author(s):  
Guido Rossi ◽  
Otto B. Zaalberg
Keyword(s):  
Immune Response ◽  
Mouse Spleen ◽  
Secondary Immune Response ◽  
Sheep Erythrocytes ◽  
Distamycin A

scholarly journals EFFECTS OF HYDROCORTISONE ON THE IN VITRO PRIMARY IMMUNE RESPONSE OF MOUSE SPLEEN CELLS TO SHEEP ERYTHROCYTES

1972 ◽  
Vol 25 (5) ◽  
pp. 345-353 ◽  
Author(s):  
MASANOBU SUGIMOTO ◽  
SHIN-ICHI TAMURA ◽  
TAKESHI KURATA ◽  
YASUYUKI EGASHIRA
Keyword(s):  
Immune Response ◽  
Primary Immune Response ◽  
Mouse Spleen ◽  
Spleen Cells ◽  
Sheep Erythrocytes

Effect of route of immunization on development of antibody-forming cells in hilar lymph nodes

1980 ◽  
Vol 30 (2) ◽  
pp. 391-396
Author(s):  
J Stein-Streilein ◽  
D A Hart
Keyword(s):  
Immune Response ◽  
Respiratory Tract ◽  
Lymph Nodes ◽  
Lower Respiratory Tract ◽  
Immunoglobulin M ◽  
Secondary Immune Response ◽  
Sheep Erythrocytes ◽  
Low Concentrations ◽  
Draining Lymph Nodes ◽  
Intraperitoneal Inoculation

Studies were designed to compare the effect of the route of inoculation of sheep erythrocytes (SRBC) on the development of antibody-forming cells (AFC) in the hilar lymph nodes of BDF1 mice. The major observation from these studies was that the immunogenicity of low concentrations of antigen was dependent on the route of inoculation. Intratracheal, intravenous, or footpad inoculation of low numbers of SRBC (10(7)) did not stimulate development of AFC in hilar lymph nodes. However, intraperitoneal inoculation of the same concentration of antigen induced an immunoglobulin M AFC response in the hilar lymph nodes. Moreover, both intraperitoneal and intratracheal inoculation of 10(7) SRBC primed the mice for an anamnestic AFC response to a subsequent intratracheal inoculation of 10(7) SRBC. These findings support the hypothesis that presentation of antigen by the local or systemic routes may induce either or both a primary a secondary immune response in the draining lymph nodes of the lower respiratory tract.

scholarly journals REGULATION OF THE IMMUNE RESPONSE

1971 ◽  
Vol 134 (3) ◽  
pp. 577-587 ◽  
Author(s):  
John W. Kappler ◽  
Michael Hoffmann ◽  
Richard W. Dutton
Keyword(s):  
Immune Response ◽  
T Cells ◽  
Mouse Spleen ◽  
Phagocytic Cells ◽  
Sheep Erythrocytes ◽  
Primary Means ◽  
In Vitro Response

The effect of passively transfered antiserum against sheep erythrocytes (SRBC) on the antigen stimulated increase of SRBC-specific plaque-forming cells (anti-SRBC-PFC) and SRBC-specific thymus-derived lymphocytes (SRBC-specific T-cells) in the mouse spleen was examined. A dose of antiserum which severely suppressed the development of anti-SRBC-PFC did not prevent the increase in SRBC-specific T-cells, as measured by their ability to cooperate in the in vitro response to trinitrophenylated (TNP) SRBC. It was shown that the insensitivity of these T-cells to antiserum could not be explained by their low antigen requirement as compared to that of PFC. In the in vivo response of mice to TNP-SRBC, antibody specific for TNP suppressed the appearance of both anti-TNP- and anti-SRBC-PFC. The presence of free SRBC specifically prevented the suppression of the anti-SRBC-PFC. These observations are consistent with opsonization by phagocytic cells as the primary means of the observed suppression of PFC development by antibody.

The influence of Bordetella pertussis on the preparation of mouse spleens for the secondary immune response

1969 ◽  
Vol 15 (7) ◽  
pp. 814-816 ◽  
Author(s):  
H. Finger ◽  
P. Emmering ◽  
E. Brüss
Keyword(s):  
Immune Response ◽  
Bordetella Pertussis ◽  
Lymphoid Tissues ◽  
Secondary Immune Response ◽  
Spleen Cells ◽  
Simultaneous Injection ◽  
Sheep Erythrocytes ◽  
Initial Injection ◽  
Erythrocyte Antigen

The simultaneous injection of 4 × 108 sheep erythrocytes and 3 × 109 killed cells of Bordetella pertussis effects an accelerated and prolonged multiplication of antibody-forming spleen cells if compared with mice immunized with the erythrocyte antigen only. If mice of both groups are given a second injection of 4 × 108 sheep erythrocytes 6 weeks later, the numbers of indirect plaque-forming cells are markedly increased in pertussis-treated mice. This is connected with enhanced 7 S serum hemolysin titers. The conclusion is drawn that an initial injection of B. pertussis prepares the lymphoid tissues of mice for the secondary immune response.

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