Chicken Embryo Lethal Orphan (CELO) Virus DNA Present in Hamster Cell Lines Derived from CELO-Induced Hepatomas

Intervirology ◽  
1978 ◽  
Vol 9 (5) ◽  
pp. 321-324 ◽  
Author(s):  
John T. May ◽  
James K. Welsh ◽  
Bonnie B. Asch ◽  
Kenneth J. McCormick
Keyword(s):  
Cell Lines ◽  
Chicken Embryo ◽  
Celo Virus

scholarly journals Repair of a previously uncharacterized second host-range gene contributes to full replication of modified vaccinia virus Ankara (MVA) in human cells

2020 ◽  
Vol 117 (7) ◽  
pp. 3759-3767 ◽  
Author(s):  
Chen Peng ◽  
Bernard Moss
Keyword(s):  
Vaccinia Virus ◽  
Host Range ◽  
Cell Lines ◽  
Chicken Embryo ◽  
A549 Cells ◽  
Human Cells ◽  
Modified Vaccinia Virus Ankara ◽  
Core Proteins ◽  
Embryo Fibroblasts ◽  
Chicken Embryo Fibroblasts

Modified vaccinia virus Ankara (MVA), a widely used vaccine vector for expression of genes of unrelated pathogens, is safe, immunogenic, and can incorporate large amounts of added DNA. MVA was derived by extensively passaging the chorioallantois vaccinia virus Ankara (CVA) vaccine strain in chicken embryo fibroblasts during which numerous mutations and deletions occurred with loss of replicative ability in most mammalian cells. Restoration of the deleted C12L gene, encoding serine protease inhibitor 1, enhances replication of MVA in human MRC-5 cells but only slightly in other human cells. Here we show that repair of the inactivated C16L/B22R gene of MVA enhances replication in numerous human cell lines. This previously uncharacterized gene is present at both ends of the genome of many orthopoxviruses and is highly conserved in most, including smallpox and monkeypox viruses. The C16L/B22R gene is expressed early in infection from the second methionine of the previously annotated Copenhagen strain open reading frame (ORF) as a 17.4-kDa protein. The C16/B22 and C12 proteins together promote MVA replication in human cells to levels that are comparable to titers in chicken embryo fibroblasts. Both proteins enhance virion assembly, but C16/B22 increases proteolytic processing of core proteins in A549 cells consistent with higher infectious virus titers. Furthermore, human A549 cells expressing codon-optimized C16L/B22R and C12L genes support higher levels of MVA replication than cell lines expressing neither or either alone. Identification of the genes responsible for the host-range defect of MVA may allow more rational engineering of vaccines for efficacy, safety, and propagation.

Virus-Specific Markers and Virus-Like Particles in Cell Lines of Tumors Produced by CELO Virus in Syrian Golden Hamsters2

1978 ◽  
Vol 61 (1) ◽  
pp. 163-171 ◽  
Author(s):  
Bonnie B. Asch ◽  
Kenneth J. McCormick ◽  
Wayne A. Stenback ◽  
John J. Trentin
Keyword(s):  
Cell Lines ◽  
Virus Like Particles ◽  
Celo Virus

scholarly journals PDTM-03. CHICKEN EMBRYO CHORIOALLANTOIC MEMBRANE (CAM) ASSAY AS A XENOGRAFT MODEL FOR TREATMENT OF DIFFUSE INTRINSIC PONTINE GLIOMAS

2019 ◽  
Vol 21 (Supplement_6) ◽  
pp. vi187-vi187
Author(s):  
Erica Power ◽  
Liang Zhang ◽  
Fabrice Lucien-Matteoni ◽  
David Daniels
Keyword(s):  
Cell Lines ◽  
Chicken Embryo ◽  
Tumor Volume ◽  
Radiation Treatment ◽  
Tumor Biology ◽  
Chorioallantoic Membrane ◽  
Xenograft Model ◽  
Cam Assay ◽  
Diffuse Intrinsic Pontine Gliomas ◽  
H3k27m Mutation

Abstract BACKGROUND Diffuse intrinsic pontine gliomas (DIPG), known today as diffuse midline gliomas with the H3K27M mutation, are inoperable and aggressive brain tumors found predominantly in children. Despite extensive research no treatment or cure has been elucidated. Studies in 2012 showed that the majority of DIPG tumors have the H3K27M mutation, but the development of novel therapeutics has been hindered by lack of good laboratory models. Although patient derived cell lines with the H3K27M mutation have increased, the development of good murine xenografts are lagging, requiring extensive time and money. Previous studies have shown that the chicken embryo Chorioallantoic Membrane (CAM) model is an affordable and time-efficient method of studying tumor biology and response to treatment. We sought to develop this in H3K27M tumors to bridge the gap between in vitro—in vivo studies. METHODS Three patient-derived H3K27M cell lines were cultured and implanted on the chick embryo CAM on day 9 post-fertilization. Tumors underwent multiple drug treatment with 0.5uM or 2.0uM Alisertib (days 11, 13, 15) or a single targeted radiation treatment at 2 Grey (day 13). Tumor volume was assessed via ultrasound on day 16. The tumor specimens were harvested and analyzed by immunohistochemistry. RESULTS Patient-derived DIPG cells implanted on the CAM maintain their molecular characteristics and form tumors on the surface of the CAM as evidenced by H&E and H3K27M status. Statistically significant differences in volume were found in three H3K27M DIPG lines in Alisertib treated tumors compared to the control (p< 0.001, p< 0.001, p= 0.005). Statistically significant differences in tumor volume were found when treating two DIPG lines with targeted radiation therapy compared to controls (p= 0.01, p= 0.02). CONCLUSIONS These results validated the CAM assay as a reliable xenograft model for studying DIPG tumor biology and determining efficacy of drug and radiation treatment.

The Chicken-Embryo-Lethal-Orphan (CELO) Virus as a Tissue-Culture Contaminant

1962 ◽  
Vol 6 (4) ◽  
pp. 406 ◽  
Author(s):  
Vance J. Yates ◽  
Dharam V. Ablashi ◽  
Pei W. Chang ◽  
Dorothy E. Fry
Keyword(s):  
Tissue Culture ◽  
Chicken Embryo ◽  
Celo Virus

Purification, Physical Features, and Electron Microscopy of Celo Virus

1970 ◽  
Vol 28 ◽  
pp. 166-167
Author(s):  
W. A. Stenback ◽  
J. P. Anderson ◽  
K. J. McCormick ◽  
J. J. Trentin
Keyword(s):  
Electron Microscopy ◽  
Chicken Embryo ◽  
Allantoic Fluid ◽  
Human Cells ◽  
Potential Candidate ◽  
Cancer Etiology ◽  
Avian Adenovirus ◽  
Celo Virus ◽  
Physical Features

Chicken-embryo-lethal-orphan (CELO) virus is an avian adenovirus shown to be oncogenic in newborn random bred and inbred hamsters (1). Transformation of human cells by this agent has been reported (2). This virus is ubiquitously present in chickens and market eggs and is therefore considered to be a potential candidate for cancer etiology in man.Virus for the present study was inoculated into 10-day CELO-free embryonated eggs, and crude stocks consisted of 72-hr allantoic fluid harvests. To 80 ml of crude stock was added 0. 8 gm Na-deoxycholate and 4. 0 ml of 0. 25% trypsin, and the mixture incubated at 37 C for 30 min.

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