Cytochemistry and Immunofluorescence of CELO Virus, an Oncogenic Avian Adenovirus, in Chicken Embryo Kidney and Chicken Embryo Fibroblastic Cell Cultures

1972 ◽  
Vol 16 (3) ◽  
pp. 558 ◽  
Author(s):  
Louise T. Miller ◽  
V. Jasty ◽  
Vance J. Yates
Keyword(s):  
Cell Cultures ◽  
Chicken Embryo ◽  
Fibroblastic Cell ◽  
Avian Adenovirus ◽  
Celo Virus

Purification, Physical Features, and Electron Microscopy of Celo Virus

1970 ◽  
Vol 28 ◽  
pp. 166-167
Author(s):  
W. A. Stenback ◽  
J. P. Anderson ◽  
K. J. McCormick ◽  
J. J. Trentin
Keyword(s):  
Electron Microscopy ◽  
Chicken Embryo ◽  
Allantoic Fluid ◽  
Human Cells ◽  
Potential Candidate ◽  
Cancer Etiology ◽  
Avian Adenovirus ◽  
Celo Virus ◽  
Physical Features

Chicken-embryo-lethal-orphan (CELO) virus is an avian adenovirus shown to be oncogenic in newborn random bred and inbred hamsters (1). Transformation of human cells by this agent has been reported (2). This virus is ubiquitously present in chickens and market eggs and is therefore considered to be a potential candidate for cancer etiology in man.Virus for the present study was inoculated into 10-day CELO-free embryonated eggs, and crude stocks consisted of 72-hr allantoic fluid harvests. To 80 ml of crude stock was added 0. 8 gm Na-deoxycholate and 4. 0 ml of 0. 25% trypsin, and the mixture incubated at 37 C for 30 min.

Comparative use of fructose and glucose in human liver and fibroblastic cell cultures

In Vitro ◽  
1984 ◽  
Vol 20 (9) ◽  
pp. 699-706 ◽  
Author(s):  
B. Delhotal ◽  
F. Lemonnier ◽  
M. Couturier ◽  
C. Wolfrom ◽  
M. Gautier ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Human Liver ◽  
Fibroblastic Cell

scholarly journals Counter-transport in chick embryo fibroblasts. A significant factor in measurement of glucose entry

1982 ◽  
Vol 206 (2) ◽  
pp. 301-309 ◽  
Author(s):  
R J Gay ◽  
H Amos
Keyword(s):  
Chick Embryo ◽  
Glucose Transport ◽  
Cell Cultures ◽  
Chicken Embryo ◽  
Primary Cell ◽  
Primary Cell Cultures ◽  
Embryo Fibroblasts ◽  
Chicken Embryo Fibroblasts ◽  
Stimulation Of

Enhanced rates of carrier-mediated 3-O-methyl-D-glucose (0.1 mM) transport were observed in primary cell cultures of chicken embryo fibroblasts deprived of glucose for 1 day. The addition of 5.5 mM-glucose, glucosamine or 2-deoxy-D-glucose for 15 min (37 degrees C) to glucose-starved cultures followed by washing and immediate measurement of 3-O-methyl-D-glucose transport resulted in an apparent further stimulation of transport. Transport stimulation increased with increasing concentrations of the added preincubation sugar and was observed at test concentrations ranging from 0.1 mM- to 10 mM-3-O-methyl-D-glucose. This enhancement occurred when the preloaded sugar was rapidly effluxing from cells and was eliminated by allowing cultures to incubate in buffer without sugar for 30 min (37 degrees C) after the removal of hexose and before measuring transport. A transient overshoot in the cumulative uptake of 3-O-methyl-D-glucose was observed in glucose-starved cultures that were pre-incubated in the presence of 55 mM-glucose or -glucosamine for 15 min (37 degrees C). These data suggest that counter-transport accounts for the apparent enhancement of glucose-transport capability observed in glucose-starved cells when they are briefly re-exposed to hexose.

A Simple Technique for Preparation of Chicken-Embryo-Skin Cell Cultures

1982 ◽  
Vol 26 (1) ◽  
pp. 182 ◽  
Author(s):  
A. Silim ◽  
M. A. S. Y. El Azhary ◽  
R. S. Roy
Keyword(s):  
Cell Cultures ◽  
Chicken Embryo ◽  
Simple Technique ◽  
Skin Cell

scholarly journals Transcriptional Organization of the Avian Adenovirus CELO

1998 ◽  
Vol 72 (11) ◽  
pp. 9278-9285 ◽  
Author(s):  
Vincent Payet ◽  
Claire Arnauld ◽  
Jean-Paul Picault ◽  
André Jestin ◽  
Patrick Langlois
Keyword(s):  
Human Adenovirus ◽  
Virus Genome ◽  
Open Reading Frames ◽  
Structural Protein ◽  
Avian Adenovirus ◽  
Recent Computer ◽  
Celo Virus ◽  
Cdna Isolation ◽  
Late Expression

ABSTRACT A detailed map of the transcriptional organization of the CELO virus genome was produced. Recent computer analysis of CELO virus has indicated the presence of 38 putative open reading frames (ORFs). This study, based on analysis of the transcriptional products of CELO in vitro, confirmed the presence of RNAs for 26 of these 38 ORFs. All of the results were obtained by cDNA isolation or specific reverse transcriptase PCR. Observation of ORF transcription kinetics postinfection revealed the existence of early and late expression, with the earliest starting at 2 h postinfection. The 5′ untranslated regions of some RNAs were also studied, and this revealed the existence of a bipartite leader sequence, comparable in structure to the tripartite leader of mastadenovirus. The leader most probably involved in transcriptional activity was observed in most of the structural protein genes of the CELO virus genome. This suggests some homology in transcriptional organization between the avian adenovirus CELO and known mastadenoviruses such as human adenovirus.

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