The Chicken-Embryo-Lethal-Orphan (CELO) Virus as a Tissue-Culture Contaminant

1962 ◽  
Vol 6 (4) ◽  
pp. 406 ◽  
Author(s):  
Vance J. Yates ◽  
Dharam V. Ablashi ◽  
Pei W. Chang ◽  
Dorothy E. Fry
Keyword(s):  
Tissue Culture ◽  
Chicken Embryo ◽  
Celo Virus

Cytoplasmic Tubules in Cells Infected with Laryngotracheitis Virus

1969 ◽  
Vol 27 ◽  
pp. 376-377
Author(s):  
A. M. Watrach
Keyword(s):  
Tissue Culture ◽  
Small Proportion ◽  
Chicken Embryo ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Morphologic Characteristics ◽  
Infectious Laryngotracheitis ◽  
Laryngotracheitis Virus ◽  
In Cells

During a study of the development of infectious laryngotracheitis (LT) virus in tissue culture cells, unusual tubular formations were found in the cytoplasm of a small proportion of the affected cells. It is the purpose of this report to describe the morphologic characteristics of the tubules and to discuss their possible association with the development of virus.The source and maintenance of the strain of LT virus have been described. Prior to this study, the virus was passed several times in chicken embryo kidney (CEK) tissue culture cells.

Whole Mount Electron Microscopy of Chicken Microchromosomes

1970 ◽  
Vol 28 ◽  
pp. 256-257
Author(s):  
Tadashi A. Okada ◽  
David E. Comings
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Chicken Embryo ◽  
Chromosome Structure ◽  
Large Size ◽  
Whole Mount ◽  
Chromatin Fibers ◽  
Embryo Fibroblasts ◽  
Chicken Embryo Fibroblasts

Although many observations have been made on the ultrastructure of chromosomes by whole mount electron microscopy, the large size of most chromosomes has made it difficult to trace the chromatin fibers throughout the entire course of a given chromatid.In the present study, microchromosomes of the chicken were used to provide information on this facet of chromosome structure. Cells from a tissue culture of chicken embryo fibroblasts were exposed to colcemid 0.1 ug/ml for 3 hours and the cells in mitosis were selectively removed by shaking with 0.05% trypsin.

scholarly journals A FINE STRUCTURAL STUDY OF ADHESIVE CELL JUNCTIONS IN HETEROTYPIC CELL AGGREGATES

1970 ◽  
Vol 47 (1) ◽  
pp. 197-210 ◽  
Author(s):  
Peter B. Armstrong
Keyword(s):  
Tissue Culture ◽  
Electron Microscope ◽  
Cell Sorting ◽  
Chicken Embryo ◽  
Cell Junctions ◽  
Cell Contact ◽  
Cell Aggregates ◽  
Undifferentiated Cell ◽  
Cell Type ◽  
Embryo Heart

Mixed suspensions of cells obtained by dissociation of 7 day chicken embryo heart and pigmented retina were allowed to reaggregate in tissue culture. The reaggregates which resulted contained both kinds of cells. Establishment of homogeneous tissues by cell sorting out in these reaggregates was advanced by 20 hr in culture and was complete within 2 days. When sorting out was advanced, heterotypic aggregates were fixed, sectioned, and examined in the electron microscope. Particular attention was paid to the morphology of regions of contact between cells. No qualitative differences were observed in the contact junctions between like cells (heart-heart or pigmented retina-pigment retina junctions) and unlike cells (heart-pigmented retina junctions). Broad areas of undifferentiated cell contact with cell membranes separated by a 100–200 A gap were formed regardless of cell type. Specialized junctions of the fascia and macula adherens type were also present, not only between like cells but also between unlike cells.

Chicken Embryo Lethal Orphan (CELO) Virus DNA Present in Hamster Cell Lines Derived from CELO-Induced Hepatomas

Intervirology ◽  
1978 ◽  
Vol 9 (5) ◽  
pp. 321-324 ◽  
Author(s):  
John T. May ◽  
James K. Welsh ◽  
Bonnie B. Asch ◽  
Kenneth J. McCormick
Keyword(s):  
Cell Lines ◽  
Chicken Embryo ◽  
Celo Virus
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