scholarly journals Evaluation of Antioxidant and DNA Damage Protection Activity of the Hydroalcoholic Extract ofDesmostachya bipinnataL. Stapf

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Upendarrao Golla ◽  
Solomon Sunder Raj Bhimathati
Keyword(s):  
Dna Damage ◽  
Antioxidant Activity ◽  
Yeast Cells ◽  
Dependent Manner ◽  
Hydroalcoholic Extract ◽  
Dose Dependent ◽  
Protection Activity ◽  
Dna Damage Protection ◽  
Damage Protection

Desmostachya bipinnataStapf (Poaceae/Gramineae) is an official drug of ayurvedic pharmacopoeia. Various parts of this plant were used extensively in traditional and folklore medicine to cure various human ailments. The present study was aimed to evaluate the antioxidant and DNA damage protection activity of hydroalcoholic extract ofDesmostachya bipinnatabothin vitroandin vivo, to provide scientific basis for traditional usage of this plant. The extract showed significant antioxidant activity in a dose-dependent manner with an IC50value of264.18±3.47 μg/mL in H2O2scavenging assay and prevented the oxidative damage to DNA in presence of DNA damaging agent (Fenton’s reagent) at a concentration of 50 μg/mL. Also, the presence of extract protected yeast cells in a dose-dependent manner against DNA damaging agent (Hydroxyurea) in spot assay. Moreover, the presence of extract exhibited significant antioxidant activityin vivoby protecting yeast cells against oxidative stressing agent (H2O2). Altogether, the results of current study revealed thatDesmostachya bipinnatais a potential source of antioxidants and lends pharmacological credence to the ethnomedical use of this plant in traditional system of medicine, justifying its therapeutic application for free-radical-induced diseases.

scholarly journals COMPARATIVE STUDY AGAINST OXIDATIVE STRESS AND DNA DAMAGE PROTECTION ACTIVITY OF THE DIFFERENT EXTRACTS OF TUBERS OF ARISAEMA TORTUOSUM (WALL.) SCHOTT.

2019 ◽  
pp. 166-170
Author(s):  
PRIYANKA CHAKRABORTY ◽  
NRIPENDRA NATH BALA ◽  
SUDIPTA DAS
Keyword(s):  
Dna Damage ◽  
Antioxidant Activity ◽  
Comparative Study ◽  
Methanolic Extract ◽  
Total Phenolic ◽  
Flavonoid Compounds ◽  
Protection Activity ◽  
Dna Damage Protection ◽  
Damage Protection

Objective: Our main aim is to evaluate the comparative in vitro antioxidant and DNA damage protection activity study with different extracts of tubers of Arisaema tortuosum. Methods: A. tortuosum (Wall.) Schott. (Araceae family) is commonly known as whipcord cobra lily, used in India as traditional medicine. In vitro, antioxidant activity was done by six methods including determination of total phenolic and total flavonoid compounds. The DNA damage was estimated by standard diphenylamine reaction. Results: Methanolic extract of tubers of A. tortuosum has been found rich in phenolic and flavonoid compounds when compared with other extracts. Methanolic extract of rhizomes of A. tortuosum showed potent antioxidant activity at a concentration of 400 μg/mL and potent the DNA damage protecting activity at a concentration of 50 μg/mL. Conclusion: Comparative study against oxidative damage and protection to the DNA damage protection activity of the different extracts of tubers of A. tortuosum (Wall.) Schott. showed that methanolic extract is most potent, though ethyl acetate extract also showed activity.

Fermented pearl millet: a product with enhanced bioactive compounds and DNA damage protection activity

2019 ◽  
Vol 13 (2) ◽  
pp. 1479-1488 ◽  
Author(s):  
Sukhvinder Singh Purewal ◽  
Kawaljit Singh Sandhu ◽  
Raj Kumar Salar ◽  
Pinderpal Kaur
Keyword(s):  
Dna Damage ◽  
Pearl Millet ◽  
Bioactive Compounds ◽  
Protection Activity ◽  
Dna Damage Protection ◽  
Damage Protection

Enhanced total phenolic and isoflavone aglycone content, antioxidant activity and DNA damage protection of soybeans processed by solid state fermentation with Rhizopus oligosporus RT-3

RSC Advances ◽  
2016 ◽  
Vol 6 (35) ◽  
pp. 29741-29756 ◽  
Author(s):  
Yu Xiao ◽  
Juan Fan ◽  
Yulian Chen ◽  
Xin Rui ◽  
Qiuqin Zhang ◽  
...  
Keyword(s):  
Dna Damage ◽  
Antioxidant Activity ◽  
Solid State ◽  
Solid State Fermentation ◽  
Total Phenolic ◽  
Rhizopus Oligosporus ◽  
Short Time ◽  
Dna Damage Protection ◽  
Damage Protection

In this study,Rhizopus oligosporusRT-3, which was first isolated in our group, was used for solid state fermentation of soybeans (R. oligosporus-fermented soybeans, RFS) in a short time (22 h).

scholarly journals Pedigree analyses of yeast cells recovering from DNA damage allow assignment of lethal events to individual post-treatment generations.

Genetics ◽  
1990 ◽  
Vol 124 (1) ◽  
pp. 57-65
Author(s):  
F Klein ◽  
A Karwan ◽  
U Wintersberger
Keyword(s):  
Dna Damage ◽  
Post Treatment ◽  
Yeast Cells ◽  
Dependent Manner ◽  
The Third ◽  
Lethal Mutations ◽  
Dna Damaging Agents ◽  
Kinetics Of ◽  
Dose Dependent ◽  
Insight Into

Abstract Haploid cells of Saccharomyces cerevisiae were treated with different DNA damaging agents at various doses. A study of the progeny of individual such cells (by pedigree analyses up to the third generation) allowed the assignment of lethal events to distinct post treatment generations. By microscopically inspecting those cells which were not able to form visible colonies we could discriminate between cells dying from immediately effective lethal hits and those generating microcolonies (three to several hundred cells) probably as a consequence of lethal mutation(s). The experimentally obtained numbers of lethal events (which we call apparent lethal fixations) were mathematically transformed into mean probabilities of lethal fixations as taking place in cells of certain post treatment generations. Such analyses give detailed insight into the kinetics of lethality as a consequence of different kinds of DNA damage. For example, X-irradiated cells lost viability mainly by lethal hits (which we call 00-fixations); only at a higher dose also lethal mutations fixed in the cells that were in direct contact with the mutagen (which we call 0-fixations), but not in later generations, occurred. Ethyl methanesulfonate (EMS)-treated cells were hit by 00-fixations in a dose dependent manner; 0-fixations were not detected for any dose of EMS applied; the probability for fixation of lethal mutations was found equally high for cells of the first and second post treatment generation and, unexpectedly, was well above control in the third post-treatment generation. The distribution of all sorts of lethal fixations taken together, which occurred in the EMS-damaged cell families, was not random.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Amaranth Leaf Extract Protects Against Hydrogen Peroxide Induced Oxidative Stress in Drosophila Melanogaster

2021 ◽  
Author(s):  
Johnmark Ndinawe ◽  
Hellen W. Kinyi
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Drosophila Melanogaster ◽  
Leaf Extract ◽  
Dependent Manner ◽  
Polyphenol Compounds ◽  
Dose Dependent

Abstract ObjectiveAmaranths leaves are rich in ascorbic acid and polyphenol compounds which have antioxidant activity. The aim of this study was to evaluate their in vivo antioxidant activity. The effect of consumption of Amaranth leaf extract on in vivo antioxidant activity, catalase enzyme activity and H2O2 induced oxidative stress in Drosophila melanogaster flies was assessed.ResultsConsumption of Amaranth leaf extract was associated with increased survival on exposure to H202 in a dose dependent manner in Drosophila melanogaster flies.

scholarly journals Study ofIn vitroantioxidant and DNA damage protection activity of a novel luteolin derivative isolated fromTerminalia chebula

2019 ◽  
Vol 13 (1) ◽  
pp. 755-763
Author(s):  
K. Soumya ◽  
Karickal R. Haridas ◽  
Jesna James ◽  
V. B. Sameer Kumar ◽  
Lincy Edatt ◽  
...  
Keyword(s):  
Dna Damage ◽  
Protection Activity ◽  
Dna Damage Protection ◽  
Damage Protection
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