Relationships between DNA damage protection activity, total phenolic content, condensed tannin content and antioxidant potential among Indian barley cultivars

2017 ◽  
Vol 11 ◽  
pp. 201-206 ◽  
Author(s):  
Raj Kumar Salar ◽  
Sukhvinder Singh Purewal ◽  
Kawaljit Singh Sandhu
Antioxidants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1214
Author(s):  
Pinderpal Kaur ◽  
Kawaljit Singh Sandhu ◽  
Sneh Punia Bangar ◽  
Sukhvinder Singh Purewal ◽  
Maninder Kaur ◽  
...  

Six different solvents were used as extraction medium (water, methanol, ethanol, acidified methanol, benzene and acetone) to check their phenolics extraction efficacy from flour of two rye cultivars. Rye extracts with different solvents were further analyzed for the estimation of phytochemicals and antioxidant properties. Different tests (TPC, TAC, DPPH, FRAP, ABTS, RPA and CTC) were performed to check the antioxidant properties and tannin contents in extracts. A bioactive profile of a rye cultivar indicated the presence of total phenolic compounds (0.08–2.62 mg GAE/g), total antioxidant capacity (0.9–6.8 mg AAE/g) and condensed tannin content (4.24–9.28 mg CE/100 g). HPLC was done to check phenolics in rye extract with the best solvent (water), which indicated the presence of Catechol (91.1–120.4 mg/100 g), resorcinol (52–70.3 mg/100 g), vanillin (1.3–5.5 mg/100 g), ferulic acid (1.4–1.5 mg/100 g), quercetin (4.6–4.67 mg/100 g) and benzoic acid (5.3 mg/100 g) in rye extracts. The presence of DNA damage protection potential in rye extracts indicates its medicinal importance. Rye flour could be utilized in the preparation of antioxidant-rich health-benefiting food products.


Author(s):  
PRIYANKA CHAKRABORTY ◽  
NRIPENDRA NATH BALA ◽  
SUDIPTA DAS

Objective: Our main aim is to evaluate the comparative in vitro antioxidant and DNA damage protection activity study with different extracts of tubers of Arisaema tortuosum. Methods: A. tortuosum (Wall.) Schott. (Araceae family) is commonly known as whipcord cobra lily, used in India as traditional medicine. In vitro, antioxidant activity was done by six methods including determination of total phenolic and total flavonoid compounds. The DNA damage was estimated by standard diphenylamine reaction. Results: Methanolic extract of tubers of A. tortuosum has been found rich in phenolic and flavonoid compounds when compared with other extracts. Methanolic extract of rhizomes of A. tortuosum showed potent antioxidant activity at a concentration of 400 μg/mL and potent the DNA damage protecting activity at a concentration of 50 μg/mL. Conclusion: Comparative study against oxidative damage and protection to the DNA damage protection activity of the different extracts of tubers of A. tortuosum (Wall.) Schott. showed that methanolic extract is most potent, though ethyl acetate extract also showed activity.


2019 ◽  
Vol 13 (2) ◽  
pp. 1479-1488 ◽  
Author(s):  
Sukhvinder Singh Purewal ◽  
Kawaljit Singh Sandhu ◽  
Raj Kumar Salar ◽  
Pinderpal Kaur

RSC Advances ◽  
2016 ◽  
Vol 6 (35) ◽  
pp. 29741-29756 ◽  
Author(s):  
Yu Xiao ◽  
Juan Fan ◽  
Yulian Chen ◽  
Xin Rui ◽  
Qiuqin Zhang ◽  
...  

In this study,Rhizopus oligosporusRT-3, which was first isolated in our group, was used for solid state fermentation of soybeans (R. oligosporus-fermented soybeans, RFS) in a short time (22 h).


2020 ◽  
Vol 16 (2) ◽  
pp. 124-132 ◽  
Author(s):  
Mahsa Souri ◽  
Alireza Shakeri

Background: Dittrichia graveolens (L.) is a strongly aromatic plant distributed in Mediterranean regions. This research concerns the optimization of the Total Phenolic Content (TPC) and the Total Tannin Content (TTC) of Dittrichia graveolens (L.) extracts using Response Surface Methodology for Ultrasound Assisted Extraction (UAE) and Microwave Assisted Extraction (MAE). Central Composite Design (CCD) was used to evaluate the effect of the solvent concentration and the extraction time, in different methods on TTC and TPC. The antioxidant activities and antibacterial activities were evaluated. Methods: The plant extracts were prepared using maceration, microwave and ultrasound assisted extraction. TPC and TTC were measured using Folin-Ciocalteu method. The antioxidant activities were studied using DPPH reagent and disc diffusion method was used to study the antibacterial activities. Results: This study showed the optimum condition for UAE was 49.96% methanol concentration and 11.2 min sonication, while for MAE was 55.44% methanol concentration and 2.26 min microwave extraction. It also indicated that MAE was the most effective method in comparison to UAE and maceration. The antioxidant activities of MAE extract (IC50=7.7mg/mL) were more than UAE extract (IC50=21.5mg/mL) and maceration (IC50=32.3mg/mL). Conclusion: As a conclusion, it was indicated that MAE was the most effective method. The higher total phenolic content caused higher antioxidant activities as MAE extract had the highest antioxidant activities. The antibacterial test showed the great potential of this plant as an antibacterial compound resource against different bacteria.


2019 ◽  
Vol 13 (1) ◽  
pp. 755-763
Author(s):  
K. Soumya ◽  
Karickal R. Haridas ◽  
Jesna James ◽  
V. B. Sameer Kumar ◽  
Lincy Edatt ◽  
...  

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
C. Priyanthi ◽  
R. Sivakanesan

Background. The present study evaluates the antioxidant properties of some Sri Lankan red rice varieties using water extracts. Methods. Water extracts of rice varieties Attakkari, Bg2907, and Bg407 were used in this study. The total antioxidant capacity was measured by ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, and reducing power assays. The total phenolic content (TPC), total flavonoid content (TFC), monomeric anthocyanin, and condensed tannin contents were measured by Folin-Ciocalteu, aluminium chloride, pH differential, and vanillin assays, respectively. Results. It was observed that mean FRAP, DPPH, reducing power, TPC, TFC, monomeric anthocyanin content, and condensed tannin content were in the range of 0.561 ± 0.113 to 0.695 ± 0.077   mmol / 100   g fresh weight (FW), 26.07 ± 3.08 to 53.66 ± 7.61   mg / mL   FW , 33.49 ± 4.105.14 to 40.81 ± 3.65   mg / mL , 0.676 ± 0.078 to 0.900 ± 0.057   mg tannic acid equivalent (TAE)/g, 5.36 ± 0.75 to 6.38 ± 0.82   mg   TAE / g   FW , 0.0202 ± 0.005 to 0.0292 ± 0.009   mg / g   FW , and 0.078 ± 0.015 to 0.104 ± 0.017   mg   TAE / g   FW , respectively. Significant differences were observed in DPPH, reducing power, and TPC among rice varieties ( p < 0.05 ). Rice variety Attakkari had the highest total antioxidant capacity (TAC), scavenging activity, reducing power, TPC, TFC, monomeric anthocyanin content, and condensed tannin content followed by Bg2907 and Bg406. Conclusion. Total phenolic compounds, total flavonoid, and condensed tannin are the major antioxidants in all three varieties of rice while the monomeric anthocyanin is only a minor antioxidant.


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