scholarly journals Extraction and Characterization of Natural Dye from Green Walnut Shells and Its Use in Dyeing Polyamide: Focus on Antibacterial Properties

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Mohammad Mirjalili ◽  
Loghman Karimi
Keyword(s):  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Natural Dye ◽  
Cupric Sulfate ◽  
Walnut Shells ◽  
Soxhlet Apparatus ◽  
Dyed Fabrics ◽  
Potassium Aluminum Sulfate ◽  
Layer Chromatography

Extraction of dyes from walnut using Soxhlet apparatus has been studied. The color components extracted and isolated from walnut shells were characterized by column chromatography, thin layer chromatography (TLC), nuclear magnetic resonance (NMR), mass spectroscopy (MS), and infrared (IR) techniques. Natural dye extract obtained from the walnut was used in dyeing polyamide fabrics with different mordants. The dyed fabrics were evaluated for antibacterial activity against pathogenic strains of Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria. As such, the relationship between antibacterial activity and dye concentration is investigated. Durability of antibacterial activity to laundering is also discussed. Results indicate that the polyamide dyed with walnut displayed excellent antibacterial activity in the presence of ferric sulfate, cupric sulfate, and potassium aluminum sulfate and exhibited good and durable fastness properties.

scholarly journals STUDIES ON BIOSURFACTANT PRODUCED USING Exiguobacterium profundum

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Nur Asni Setiani ◽  
Welly Octaviyani ◽  
Syarif Hamdani ◽  
Irma Mardiah
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Pharmaceutical Preparations ◽  
Initial Screening ◽  
Gram Negative ◽  
Emulsification Index ◽  
Exiguobacterium Profundum ◽  
Layer Chromatography

Background: The manufacture of pharmaceutical preparations generally adds surfactants. Microbial biosurfactants can be an alternative because biodegradable and have antibacterial properties.   Objective: This study aimed to examine the biosurfactant activity of Exiguobacterium profundum.Methods: Hemolysis and spreading oil tests were performed as an initial screening. Biosurfactant production was carried out by growing bacteria on oil-enriched media with shaker system for 7 days. Biosurfactant activity can be seen from the emulsification index, while the characterization of biosurfactant were used thin layer chromatography and antibacterial qualitative testing.Results: Exiguobacterium profundum could spread the oil layer and form micelles. The emulsification index on days 0, 1, 3, 5, and 7 showed percentage in sequence 44.83%, 48.28%, 48.28%, 40%, and 43.75%. The result of TLC showed lipopeptide group which is marked with red stain with ninhydrin appearance. Antibacterial testing using Escherichia coli showed the formation of clear zones around the disk paper.Conclusion: The biosurfactant produced by Exigoubacterium profundum can be classified into lipopeptide group which has antibacterial activity against gram-negative.

scholarly journals Antibacterial Properties of Biofilm Schiff Base Derived from Dialdehyde Cellulose and Chitosan

2019 ◽  
Vol 19 (2) ◽  
pp. 405
Author(s):  
Agung Pratama ◽  
Firman Sebayang ◽  
Rumondang Bulan Nasution
Keyword(s):  
Tensile Strength ◽  
Antibacterial Activity ◽  
Schiff Base ◽  
Antibacterial Properties ◽  
Oxidized Cellulose ◽  
Natural Polymers ◽  
Dialdehyde Cellulose ◽  
Ft Ir ◽  
Ir Analysis

Cellulose and chitosan are natural polymers that have been used as biocomposite. The aim of this research is to obtain biofilms from chitosan and oxidized cellulose crosslinks. This research is divided into three steps, i.e., isolation of cellulose from oil palm trunk and oxidation of cellulose using NaIO4 (0.2; 0.4; 0.6; 0.8; 1.0 mg/mL) to obtain dialdehyde cellulose (DAC), crosslink of oxidized cellulose with chitosan (DD = 72.85%) to obtain biofilm of chitosan/DAC (CDAC), and characterization of biofilms. The crosslinked reaction was confirmed by FT-IR analysis that showed the spectrum of Schiff base C=N group at 1651 cm–1. Tensile strength increased gradually when the NaIO4 concentration used was 0.2–0.6 mg/mL, but after those concentrations, the tensile strength slightly decreased. The morphology analysis showed that CDAC had smoother morphology than DAC, which was shown rough and showed some particle indicated the presence of unreacted cellulose. CDAC biofilms that prepared with 1.0 mg/mL NaIO4 showed the greatest antibacterial activity.

scholarly journals NOVEL COMPOSITE OF SILVER-BACTERIAL CELLULOSE (Ag-BC) FROM SIWALAN SAP (Borassus flabellifer) AND ITS ANTIBACTERIAL ACTIVITY

2020 ◽  
Vol 83 (1) ◽  
pp. 19-25
Author(s):  
Suntini Suntini ◽  
Anastasia Wheni Indrianingsih ◽  
Harjono Harjono
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Cellulose ◽  
Antibacterial Properties ◽  
Reduction Process ◽  
Diffusion Method ◽  
Natural Material ◽  
Gram Positive Bacteria ◽  
Antibacterial Assay ◽  
Natural Composite

Recently, a wound healing from natural composite with excellent properties is in a high demand. In this study, a novel composite of bacterial cellulose made from Siwalan sap (Borassus flabellifer) was achieved. Siwalan is a common plant in Java Island of Indonesia and the application is very limited for beverage only. This study aims to determine the effect of the AgNO3/NaBH4 concentration ratio in the development of Ag-BC composites and its antibacterial properties from Siwalan sap. Ag-BC composites were prepared by impregnating the silver solution into the BC matrix through the reduction process with NaBH4. Characterization of Ag-BC composites conducted using Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and Energy Dispersive X-ray (EDX). Antibacterial assay was performed using disc diffusion method against Salmonella typhimurium (Gram-negative) and Staphylococcus aureus (Gram-positive) bacteria. The characterization of Ag-BC composite shows the nanostructure of BC with a length of fiber around 35-60 nm in width. The SEM-EDX micrograph showed that silver particles were impregnated into the BC matrix. Antibacterial activity test results showed that the Ag-BC composite had the ability to inhibit the bacteria S. typhimurium and S. aureus with good inhibition. This result showed the potential application of Ag-BC composite from Siwalan plant as a natural material for medical and pharmaceutical purpose, especially as an antibacterial agent.

scholarly journals Antibacterial Activity and Drug Loading of Moxifloxacin-Loaded Poly(Vinyl Alcohol)/Chitosan Electrospun Nanofibers

2021 ◽  
Vol 8 ◽  
Author(s):  
Qi Liu ◽  
Wen-Chong Ouyang ◽  
Xiu-Hong Zhou ◽  
Tao Jin ◽  
Zheng-Wei Wu
Keyword(s):  
Antibacterial Activity ◽  
Vinyl Alcohol ◽  
Composite Nanofibers ◽  
Drug Loading ◽  
Antibacterial Properties ◽  
Electrospun Nanofibers ◽  
Poly Vinyl Alcohol ◽  
X Ray Diffraction ◽  
Blank Group

In this study, nanofibers with different ratios of poly(vinyl alcohol) and chitosan incorporated with moxifloxacin hydrochloride (MH/PVA/CS) were fabricated through the blending electrospinning, and the morphological features were tested using scanning electron microscopy (SEM). Further characterization of the new nanofiber was accomplished by Thermogravimetric analysis (TG), X-ray diffraction (XRD) and Fourier-transform infrared spectroscopy (FTIR). Antibacterial activity of the MH-loaded nanofibers at different drug loading were tested and compared with the blank group. Experimental results show that the MH/PVA/CS nanofibers exhibited the good antibacterial properties against Staphylococcus aureus and Pseudomonas aeruginosa due to the MH incorporation. Compared with blank nanofibers, MH/PVA/CS nanofibers have significantly better antibacterial properties, and different proportions of PVA and CS have a certain effect on the antibacterial activity of nanofibers. The conclusions in this paper show that MH/PVA/CS composite nanofibers may have great potential in antibacterial materials.

scholarly journals Synthesis and Characterization of Selenium Nanoparticles-Lysozyme Nanohybrid System with Synergistic Antibacterial Properties

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Mahsa Vahdati ◽  
Tahereh Tohidi Moghadam
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Growth ◽  
Biomedical Applications ◽  
Antibacterial Properties ◽  
Complex Form ◽  
Vital Role ◽  
Selenium Nanoparticles ◽  
Low Concentrations ◽  
Very High

AbstractIn the light of promising potency of selenium nanoparticles in biomedical applications, this is the first study to report the synergistic antibacterial activity of these nanoparticles and lysozyme. The nanohybrid system was prepared with various concentrations of each component. Resistance of Escherichia coli and Staphylococcus aureus was compared in the presence of individual Nano and Bio counterparts as well as the nanohybrid system. Upon interaction of SeNPs with Lysozyme, the nanohybrid system efficiently enhanced the antibacterial activity compared to the protein. Therefore, SeNPs play an important role in inhibition of bacterial growth at very low concentrations of protein; whereas very high amount of the protein is required to inhibit bacterial growth individually. On the other hand, lysozyme has also played a vital role in antibacterial property of SeNPs, inducing 100% inhibition at very low concentration of each component. Hence, presence of both nano and bio counterparts induced vital interplay in the Nanohybrid system. The aged samples also presented good stability of SeNPs both as the intact and complex form. Results of this effort highlight design of nanohybrid systems with synergistic antibacterial properties to overcome the emerging antibiotic resistance as well as to define fruitful applications in biomedicine and food safety.

scholarly journals Antibacterial dyeing of polyamide using turmeric as a natural dye

2013 ◽  
Vol 13 (2) ◽  
pp. 51-56 ◽  
Author(s):  
Mohammad Mirjalili ◽  
Loghman Karimi
Keyword(s):  
Antibacterial Activity ◽  
Aluminum Sulfate ◽  
Curcuma Longa ◽  
Cupric Sulfate ◽  
Bacteriostatic Activity ◽  
Fastness Properties ◽  
Potassium Aluminum Sulfate ◽  
Escherichia Coli Bacteria ◽  
Dyed Fabric ◽  
The Relationship

Abstract Curcuma longa rhizome (turmeric) is a medicinal plant used for fabric and food coloration. In this study, polyamide (nylon 6.6) fabric was dyed with different mordants at various turmeric concentrations. The dyed fabric was evaluated for bacteriostatic activity against pathogenic strains of Gram-positive (Staphylococcus aureus) and Gramnegative (Escherichia coli) bacteria. The relationship between bacteriostatic activity and turmeric concentration was investigated. Durability of antibacterial activity to laundering is also discussed. Results indicate that the polyamide dyed with turmeric displayed excellent antibacterial activity in the presence of ferric sulfate, cupric sulfate, and potassium aluminum sulfate, and exhibited good and durable fastness properties.

scholarly journals Preparation and characterization of Copper Chitosan Nanocomposites with Antibacterial Activity for Applications in Tissue Engineering

2017 ◽  
Author(s):  
◽  
K. Maldonado-Lara
Keyword(s):  
Tissue Engineering ◽  
Antibacterial Activity ◽  
Potential Application ◽  
Antibacterial Properties ◽  
Hydroxyl Groups ◽  
Liquid Media ◽  
Solution Blending ◽  
Nanoparticles Dispersion ◽  
Bacterial Penetration

The Present work describes the preparation of nanocomposites based on chitosan (QS)/copper nanoparticles (nCu) with antibacterial properties and potential application in tissue engineering. For this purpose, nanocomposites were prepared by solution blending with ultrasound assisted, aiming to increase the nanoparticles dispersion in the biopolymer. FTIR analyses demonstrates that nCu supported in QS increase their interaction of nanoparticles with amine/hydroxyl groups of QS molecule. UV-Vis analyses demonstrates that QS/nCu nanocomposites have an absorption signal associated with the presence of nanoparticles and the possible Cu2+ ions release in liquid media. AFM analyses shown that hydrated QS form a mesh with micro pores, improving the bacterial penetration and the direct contact with nCu. This behavior was corroborated by antibacterial assays, where QS/nCu nanocomposites shown an antibacterial activity higher than 90% between 90-180 minutes of interaction. Our results suggest that is possible to obtain combined antibacterial/biocompatible nanomaterials with potential application in tissue engineering.

scholarly journals FRAKSI ETANOL, KLOROFORM, DAN N-HEKSAN BUNGA KAMBOJA PUTIH (Plumeria acuminata L.) SEBAGAI ANTIBAKTERI TERHADAP Escherichia coli dan Staphylococcus aureus DENGANBIOAUTOGRAFI

1970 ◽  
pp. 111-122
Author(s):  
Yuli Wahyu Trimulyani
Keyword(s):  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Inhibition Zone ◽  
Bacterial Suspension ◽  
Chloroform Fraction ◽  
Contact Method ◽  
Fractionation Process ◽  
E Coli ◽  
Layer Chromatography ◽  
Ethanol Fraction

Frangipani flowers (Plumeria acuminata L.) Flower is one of the plants that has antibacterial properties. Compounds that act as antibacterial are flavonoids and tannins. The purpose of this study was to prove the antibacterial activity of ethanol, chloroform, and n-hexane fractions of Frangipani flowers (Plumeria acuminata L.) flower as antibacterial against E. coli and S. aureus with Bioautography.The process of extracting plumeria acuminata L. flowers was carried out by maceration method using 70% ethanol. The extract was continued with the fractionation process with ethanol, n-hexane and chloroform solvents. Antibacterial activity testing using the well method with concentrations of 20%, 40%, 60%, 80%, 100%, positive chloramphenicol control, and negative aquadest control. The compound content test used the Thin Layer Chromatography (TLC) method with the mobile phase of chloroform: methanol: water (2:5:3) (v/v).Bioautography test using the contact method, namely the elution TLC plate, was placed on the NA medium containing a bacterial suspension for 3 hours. The biggest antibacterial test results of ethanol fraction of plumeria acuminata L. flowers were at a concentration of 100% with a diameter of inhibition zone of 13.55 mm in E. coli while 14.15 mm S. aureus. The results of TLC showed the presence of flavonoids with a price of Rf 0.70 and tannin at a price of Rf 0.68. Bioautography results showed a inhibition zone with a price of Rf 0.70 in E. coli and S. aureus which are flavonoid compounds. Based on the results of the research that has been done it can be concluded that the ethanol fraction of white frangipani flowers has antibacterial activity against E.coli and S. aureus, while the chloroform fraction and n-hexane fraction do not have antibacterial activity. The active compounds that act as antibacterial are flavonoids and tannin, but the ones that provide antibacterial effects are flavonoids with an Rf value of 0.70. Keywords: Bioautography,Escherichiacoli, Frangipani flowers, Staphylococcusaureus, TLC

scholarly journals Bio-Guided Fractionation of Retama raetam (Forssk.) Webb & Berthel Polar Extracts

Molecules ◽  
2021 ◽  
Vol 26 (19) ◽  
pp. 5800
Author(s):  
Mariem Saada ◽  
Hanen Wasli ◽  
Inès Jallali ◽  
Rim Kboubi ◽  
Karl Girard-Lalancette ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
Antibacterial Properties ◽  
Chemical Characterization ◽  
Polar Compounds ◽  
Bioactive Molecules ◽  
Prenylated Flavonoids ◽  
Retama Raetam ◽  
Polar Extracts ◽  
Layer Chromatography

The fractionation of the methanolic extract (MeOH-E) of Retama raetam (Forssk.) Webb & Berthel and further analysis by thin layer chromatography resulted in four fractions (F1, F2, F3 and F4) that, in parallel with the MeOH-E, were screened for antioxidant, cytotoxic, antidiabetic and antibacterial properties. In addition, chemical characterization of their bioactive molecules was performed using LC-DAD-ESI/MSn. The results indicated that F3 was the most promising regarding antioxidant and cytotoxicity abilities, possibly due to its richness in flavonoids class, particularly isoflavones. In turn, F1 was characterized by the presence of the most polar compounds from MeOH-E (organic acids and piscidic acid) and showed promising abilities to inhibit α-amylase, while F4, which contained prenylated flavonoids and furanoflavonoids, was the most active against the tested bacteria. The gathered results emphasize the distinct biological potentials of purified fractions of Retama raetam.

scholarly journals PRELIMINARY PHYTOCHEMICAL EVALUATION AND SPECTRAL CHARACTERIZATION OF ACTIVE CONSTITUENTS IN THE DRIED EXTRACTS OF THE WHOLE PLANT ARGYREIA IMBRICATA (ROX) SANT AND PATEL

2019 ◽  
pp. 56-60
Author(s):  
SEBASTIN V ◽  
GOPALAKRISHNAN G ◽  
SREEJITH M ◽  
ANOOB KUMAR KI
Keyword(s):  
Column Chromatography ◽  
Spectral Characterization ◽  
Whole Plant ◽  
Soxhlet Apparatus ◽  
Spectral Evaluation ◽  
Pharmacological Screening ◽  
Dried Extract ◽  
Layer Chromatography ◽  
Tlc Separation

Objective: The present study was designed with the aim of preliminary phytochemical evaluation and spectral characterization phytochemicals present in the extracts of whole plant Argyreia imbricata (Rox) Sant and Patel. Methods: The whole plant A. imbricata was collected, authenticated, and dried in the shade for powdering in mechanical grinder. The powdered plant material was extracted with Soxhlet apparatus using different solvents, and the dried extract obtained was subjected to preliminary phytochemical evaluation, and then column chromatography separation and the separated fractions were subjected to thin-layer chromatography (TLC) separation. Based on the yield in TLC, the selected compounds were subjected to carbon-13 nuclear magnetic resonance, proton NMR (1H NMR), and mass spectroscopic evaluation. Results: In the preliminary phytochemical evaluation, the presence of alkaloids, glycosides, phenolic compounds, flavonoids, carbohydrates, saponins, sterols, and terpenoids was found in different extracts. In column chromatography separation, totally 650 fractions were collected, and four compounds were isolated with the help of TLC. Among the four, two compounds were selected for spectral evaluation based on the yield obtained. The selected compounds were identified and named as, 3-ethyl-5-(hydroxymethyl) phenol and methyl 4-hydroxybenzoate with the help of spectral evaluation. Conclusion: The outcome of this study is beneficial, and further investigation in the future directed toward the detailed pharmacological screening of the extracts may give more valuable results.

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