scholarly journals FRAKSI ETANOL, KLOROFORM, DAN N-HEKSAN BUNGA KAMBOJA PUTIH (Plumeria acuminata L.) SEBAGAI ANTIBAKTERI TERHADAP Escherichia coli dan Staphylococcus aureus DENGANBIOAUTOGRAFI

1970 ◽  
pp. 111-122
Author(s):  
Yuli Wahyu Trimulyani
Keyword(s):  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Inhibition Zone ◽  
Bacterial Suspension ◽  
Chloroform Fraction ◽  
Contact Method ◽  
Fractionation Process ◽  
E Coli ◽  
Layer Chromatography ◽  
Ethanol Fraction

Frangipani flowers (Plumeria acuminata L.) Flower is one of the plants that has antibacterial properties. Compounds that act as antibacterial are flavonoids and tannins. The purpose of this study was to prove the antibacterial activity of ethanol, chloroform, and n-hexane fractions of Frangipani flowers (Plumeria acuminata L.) flower as antibacterial against E. coli and S. aureus with Bioautography.The process of extracting plumeria acuminata L. flowers was carried out by maceration method using 70% ethanol. The extract was continued with the fractionation process with ethanol, n-hexane and chloroform solvents. Antibacterial activity testing using the well method with concentrations of 20%, 40%, 60%, 80%, 100%, positive chloramphenicol control, and negative aquadest control. The compound content test used the Thin Layer Chromatography (TLC) method with the mobile phase of chloroform: methanol: water (2:5:3) (v/v).Bioautography test using the contact method, namely the elution TLC plate, was placed on the NA medium containing a bacterial suspension for 3 hours. The biggest antibacterial test results of ethanol fraction of plumeria acuminata L. flowers were at a concentration of 100% with a diameter of inhibition zone of 13.55 mm in E. coli while 14.15 mm S. aureus. The results of TLC showed the presence of flavonoids with a price of Rf 0.70 and tannin at a price of Rf 0.68. Bioautography results showed a inhibition zone with a price of Rf 0.70 in E. coli and S. aureus which are flavonoid compounds. Based on the results of the research that has been done it can be concluded that the ethanol fraction of white frangipani flowers has antibacterial activity against E.coli and S. aureus, while the chloroform fraction and n-hexane fraction do not have antibacterial activity. The active compounds that act as antibacterial are flavonoids and tannin, but the ones that provide antibacterial effects are flavonoids with an Rf value of 0.70. Keywords: Bioautography,Escherichiacoli, Frangipani flowers, Staphylococcusaureus, TLC

scholarly journals ANALISIS BIOAUTOGRAFI DAN KARAKTERISASI DENGAN FTIR PADA FRAKSI DAUN LABU SIAM (Sechium edule (jacq).SW) TERHADAP Porphyromonas gingivalis DAN Streptococcus mutans

1970 ◽  
pp. 55-66
Author(s):  
Laila Susanti ◽  
Isbiyantoro Isbiyantoro ◽  
Septiana Simanjuntak
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Mutans ◽  
Porphyromonas Gingivalis ◽  
Antibacterial Properties ◽  
Inhibition Zone ◽  
Bacterial Suspension ◽  
Chloroform Fraction ◽  
Contact Method ◽  
Fractionation Process ◽  
Sechium Edule

Siamese pumpkin leaves are one of the plants that have antibacterial properties. Compounds that act as antibacterial are flavonoite, tannin, triterpenes, alkaloids, and saponins. The aim of this study was to prove the antibacterial activity of ethanol hexane and chloroform fraction of pumpkin siam leaves and characterize the functional groups of active compounds from pumpkin siam leaves (Sechium edule (jacq). SW) with FTIR spectrophotometers. The extraction process of Siamese Pumpkin leaves was carried out by maceration method using 70% ethanol. The extract was continued with the fractionation process with ethanol, n-hexane and chloroform solvents. Antibacterial activity testing using the well method with a concentration of 100%, positive control chlorhexidi and negative control aquadst. The compound content test used the Thin Layer Chromatography (TLC) method with the stationary phase using silica plate G60 F254 and comparison of the mobile phase of chloroform: methanol: water (2: 5: 3) (v / v). Bioautography test using the contact method, namely the elution TLC plate, was placed on the NA medium containing a bacterial suspension for 3 hours. Antibacterial test results The largest ethanol fraction of pumpkin leaves was at a concentration of 100% with a diameter of inhibition zone of 11.49 mm on P. gingivalis while 16.04 mm in S. mutans. The results of TLC showed the presence of flavonoids with a price of Rf 0.47 and tannin with a price of Rf 0.84. Bioautography results showed a inhibition zone with a price of Rf 0.49 in the bacteria P. gingivalis and S. mutans which are flavonoid compounds. Characterization by FTIR spectrophotometer O-H phenol, C = C aromatic, C-O-C ether, aromatic C-H functional group Keywords: (Sechium edule (jacq) .SW). Porphyromonas Gingivalis, Streptococcus mutans, TLC, Bioautography and FTIR

Antimicrobial Activity and Hardness of as Cast and as Homogenized Cu-Ti Alloys

2015 ◽  
Vol 1130 ◽  
pp. 136-140 ◽  
Author(s):  
Dinni Nurhayani ◽  
Akhmad Ardian Korda
Keyword(s):  
Stainless Steel ◽  
Antibacterial Activity ◽  
Pure Copper ◽  
Antibacterial Properties ◽  
Inhibition Zone ◽  
Bacterial Suspension ◽  
Ti Alloy ◽  
Steel Aisi ◽  
Antibacterial Susceptibility ◽  
Microstructure Effect

Cu-Ti alloy, pure copper, and stainless steel AISI 304 were tested in this study for their microstructure, effect of alloying element to hardness, and antibacterial activity. The antibacterial properties were tested against E. coli suspensions. The titanium in Cu-Ti alloy was varied 1-4 wt. %, and the microstructure was modified by homogenization treatment. The antibacterial susceptibility test was done by inhibition zone measurement and bacterial suspension inoculation methods. Result showed the addition of titanium increased hardness from pure copper (44.93 HV) to 79.68-207.6 HV. No inhibition zone was observed for the stainless steel samples, while the largest inhibition zone diameter was gained by pure copper (12.4 mm). The killing bacteria activity test concluded that the compositions of the alloys were not directly linked to the antibacterial activity.

Antibacterial Activity of a Chitosan Derivative Obtained in the Absence of a Solvent

2016 ◽  
Vol 869 ◽  
pp. 869-873 ◽  
Author(s):  
Luizângela Reis Osório ◽  
Idglan Sá Lima ◽  
Humberto Medeiros Barreto ◽  
Josy Anteveli Osajima ◽  
Edson Cavalcanti Silva Filho
Keyword(s):  
Antibacterial Activity ◽  
Chemical Change ◽  
Antibacterial Properties ◽  
Infrared Spectrometry ◽  
Contact Method ◽  
Direct Reaction ◽  
Chitosan Derivative ◽  
E Coli ◽  
Resistant Strains ◽  
Drug Resistant Strains

A novel chitosan derivative was prepared through direct reaction of pure chitosan with acetylacetone in the absence of a solvent, and it was characterized by elemental analysis, Fourier transform infrared spectrometry (FTIR), and 13C Nuclear Magnetic Ressonance (NMR) spectroscopy. Moreover, the antibacterial properties of the new biomaterial were tested by the direct contact method against multi-drug resistant strains of Staphylococcus aureus and Escherichia coli. The results from the characterization were consistent with the modification of the chemical structure made. The new derivative showed a better antibacterial activity than raw chitosan against E. coli strains, indicating that incorporation of imine link (Schiff base) enhanced its antibacterial activity against Gram-negative bacterium. On the contrary, this chemical change did not decrease its antibacterial activity against Gram-positive bacterium.

AKTIVITAS ANTIBAKTERI DARI EKSTRAK JAMUR LAUT

2016 ◽  
Vol 1 (1) ◽  
pp. 21-26
Author(s):  
Nur Fadilah ◽  
Erawati Erawati ◽  
Hendrik Nahar ◽  
Didit Kustantio Dewanto
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Marine Fungi ◽  
Inhibition Zone ◽  
Ethyl Acetate Fraction ◽  
Raw Material ◽  
Antibacterial Compounds ◽  
E Coli ◽  
Ethanol Fraction ◽  
Inhibit Concentration

A search new antibacterial compounds should continue to be done as a raw material of antibiotics. The purpose of this study, which is to get kind of marine fungi antibacterial activity and get the value of MIC and MBC. The study was conducted from the sampling sponge, marine fungi isolation, extraction, preparation of test bacteria, antibacterial activity testing and analysis of MIC and MBC. Data was analyzed by measuring the diameter of inhibition zone are formed. The results obtained, that debris ethanol fraction from marine fungi J3 can inhibit the growth of E. coli and S. aureus at a concentration of 100 mg/L to 100.000 mg/L. At the debris ethyl acetate fraction able to inhibit concentration of 1.000 mg/L to 100.000 mg/L. At the filtrate ethyl acetate fraction was able to inhibit 10.000 mg/L to 100.000 mg/L. For both types of test bacteria, MIC and MBC values are indicated by the highest debris ethanol fraction and lowest by the fraction of ethyl acetate filtrate.

Preparation of a novel positively charged nanofiltration composite membrane incorporated with silver nanoparticles for pharmaceuticals and personal care product rejection and antibacterial properties

2016 ◽  
Vol 73 (8) ◽  
pp. 1910-1919 ◽  
Author(s):  
Zhong-Hua Huang ◽  
Yan-Na Yin ◽  
Gu-li-mi-la Aikebaier ◽  
Yan Zhang
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Photoelectron Spectroscopy ◽  
Pure Water ◽  
Antibacterial Properties ◽  
Inhibition Zone ◽  
Personal Care ◽  
X Ray ◽  
E Coli ◽  
Positively Charged

A novel positively charged N-[(2-hydroxy-3-trimethylammonium)propyl] chloride chitosan (HTCC)-Ag/polyethersulfone (PES) composite nanofiltration membrane was easily prepared by coating the active layer, HTCC, onto PES as the support through epichlorohydrin as the cross-linking reagent and nano-Ag particles as the introduced inorganic components. Scanning election microscopy, X-ray photoelectron spectroscopy, atomic force microscopy, and X-ray diffraction were employed to characterize the morphology of the resultant membranes, of which the molecular weight cut-off was about 941 Da. At 25 °C, the pure water permeability is 16.27 L/h·m2·MPa. Our results showed that the rejection of pharmaceuticals and personal care products (PPCPs) followed the sequence: atenolol > carbamazepine > ibuprofen, confirming that the membranes were positively charged. The antibacterial properties of the membranes were compared to elucidate the existence of Ag nanoparticles which help to improve antibacterial activity against Gram-negative Escherichia coli (DH5α, Rosetta) and Gram-positive Bacillus subtilis. The inhibition zone diameters of HTCC-Ag/PES membranes towards E. coli DH5α, E. coli Rosetta and Bacillus subtilis were 17.77, 16.18, and 15.44 mm, respectively. It was found that HTCC-Ag/PES membrane has a better antibacterial activity against E. coli than against Bacillus subtilis, especially for E. coli DH5α.

scholarly journals Gram-scale synthesis of splat-shaped Ag–TiO2 nanocomposites for enhanced antimicrobial properties

2020 ◽  
Vol 11 ◽  
pp. 1119-1125
Author(s):  
Mohammad Jaber ◽  
Asim Mushtaq ◽  
Kebiao Zhang ◽  
Jindan Wu ◽  
Dandan Luo ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Inhibition Zone ◽  
Multidrug Resistant ◽  
Bacterial Strains ◽  
Gram Negative ◽  
E Coli ◽  
Contagious Diseases ◽  
Good Biocompatibility

The control over contagious diseases caused by pathogenic organisms has become a serious health issue. The extensive usage of antibiotics has led to the development of multidrug-resistant bacterial strains. In this regard, metal-oxide-based antibacterial nanomaterials have received potential research interest due to the efficient prevention of microorganism growth. In this study, splat-shaped Ag–TiO2 nanocomposites (NCs) were synthesized on the gram scale and the enhanced antibacterial properties of TiO2 in the presence of silver were examined. The formation of Ag–TiO2 NCs was analyzed through various characterization techniques. The cell viability experimental results demonstrated that the Ag–TiO2 NCs have good biocompatibility. The antibacterial activity of the prepared Ag–TiO2 NCs was tested against the Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Escherichia coli (E. coli) bacterial strains. The Ag–TiO2 NCs exhibited promising and superior antibacterial properties compared to TiO2 nanospheres as confirmed by the bacterial growth and inhibition zone. The improvement in the antibacterial activity was attributed to the synergistic effect of the hybrid nature of TiO2 nanoparticles in the presence of Ag.

scholarly journals Antibacterial Activity of Lidah Mertua (Sansevieria Trifasciata Prain.) Leaves Extract on Escherichia coli and Staphylococcus aureus

2019 ◽  
Vol 7 (22) ◽  
pp. 3882-3886
Author(s):  
Yessi Febriani ◽  
Vriezka Mierza ◽  
Novi Putri Handayani ◽  
Surismayanti Surismayanti ◽  
Ibrenaita Ginting
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Chemical Compounds ◽  
Inhibition Zone ◽  
Chromatography Method ◽  
E Coli ◽  
Layer Chromatography ◽  
And Staphylococcus Aureus

BACKGROUND: Infection is the most common diseases in developing country, including Indonesia. Bacteria that often causes infection is Escherichia coli and Staphylococcus aureus. One of the traditional plants that can be used as an antibacterial is lidah mertua. AIM: The purpose of this study was to find out the profile of chemical compounds by thin layer chromatography method and determine the antibacterial activity of Lidah Mertua leaves by in vitro. METHODS: This research conducted an experimentally using non-polar, semipolar, and polar as solvents to get extract against E. coli and S. aureus as bacterial testing. The antibacterial activity using agar diffusion method to get minimum inhibitory concentration (MIC). RESULTS: The result of the research on thin layer chromatography showed that the compounds contained in the Lidah Mertua leaves were polifenol, steroids and alkaloids. The data obtained were tabulated and analysed descriptively. The antibacterial activity show that n-hexane extract does not provide inhibitory activity. MIC value show that aethyl acetate extract of lidah mertua leaves inhibited the growth of E. coli and S. aureus at concentration 50 mg/mL and 25 mg/mL with diameters of inhibition zone is 8.50 mm and 8.20 mm and methanol extract of lidah mertua leaves inhibited the growth of E. coli and S. aureus at concentration 12.5 mg/mL and 25 mg/mL with diameters of inhibition zone is 8.46 mm and 8.32 mm. CONCLUSION: The profile of chemical compounds by thin layer chromatography method showed that the compounds contained in the Lidah Mertua leaves were polifenol, steroids and alkaloids. The antibacterial activity show that n-hexane extract does not provide inhibitory activity, but aethyl acetate extract of lidah mertua leaves inhibited the growth of E. coli and S. aureus.

scholarly journals Antibacterial Activity of Aloe Vera Gel-based Edible Coating with the addition of Gum Arabic and Ascorbic Acid

2021 ◽  
Vol 5 (1) ◽  
pp. 1-4
Author(s):  
Ni Made Defy Janurianti ◽  
I Made Supartha Utama ◽  
Ida Bagus Wayan Gunam
Keyword(s):  
Antibacterial Activity ◽  
Aloe Vera ◽  
Antibacterial Properties ◽  
Inhibition Zone ◽  
Edible Coating ◽  
Diffusion Method ◽  
Heating Process ◽  
Antibacterial Compounds ◽  
E Coli ◽  
Aloe Vera Gel

Aloe vera gel has antibacterial properties. The content of antibacterial compounds in aloe vera gel is saponins, anthraquinones, tannins, aloin, and acemannan. Aloe vera gel has the potential as an edible coating for food products. The Processing carried out goes through a heating process that does think to damage the antibacterial compounds in the aloe vera gel. This study aims to determine the antibacterial activity of aloe vera gel formulated as an edible coating on S. aureus, S. mutans, E. coli and K. pneumoniae bacteria. The research method used is the disc diffusion method. Based on the test results, pure aloe vera gel has a larger inhibition zone diameter than aloe vera gel processed into an edible coating. Diameter of pure aloe vera gel inhibition zone in S. aureus, S. mutans, E. coli, and K. pneumoniae bacteria. respectively 10.966 ± 0.573 mm; 11.806 ± 0.215 mm; 10,860 ± 0.675 mm, and 10.686 ± 0.081 mm. Aloe vera gel formulated into an edible coating with antibacterial ability, namely at a concentration of 100%, which has anti activity with the inhibition zone's diameter in S. aureus, S. mutans E. coli, and K. pneumoniae bacteria, respectively. is 10,470 ± 0.213 mm; 10,673 ± 0.127 mm; 10,113 ± 0.040 mm, and 9.676 ± 0.604 mm.

scholarly journals Phytochemical Screening and Antibacterial Activity of Acorus calamus L. Extracts

2019 ◽  
Vol 4 (1) ◽  
pp. 68-78
Author(s):  
Bayyinatul Muchtaromah ◽  
Alfiah Hayati ◽  
Erna Agustina
Keyword(s):  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Common Species ◽  
Chloroform Extract ◽  
Hexane Extract ◽  
Acorus Calamus ◽  
E Coli ◽  
Microdilution Method

  Staphylococcus aureus and Escherichia coli are among the most common species of gram-positive and gram-negative bacteria, which cause vaginitis, in infertile women. The Calamus rhizome (Acorus calamus L.) is an Indonesian plant that has antibacterial properties that can be used to treat vaginitis and increase fertility. The aim of this study was to determine the phytochemical and antibacterial activity of the calamus rhizoma in polar, semi-polar and non-polar solvents in the growth of S. aureus and E. coli. The antibacterial activity test was in the form of inhibitory test using the Kirby-Bauer, Minimum Inhibi-tion Concentration (MIC) and Minimum Bactericidal Concentration (MBC) by microdilution method with multilevel dilution (concentra-tions 50; 25; 12.5; 6.25; 3.13; 1.56; 0.78; and 0.39%). The screening results showed that ethanol and n-hexane extract contained alkaloids and triterpenoids, while chloroform extract was only triterpenoid. Chloroform extract produced the largest inhibition zone diameter of S. aureus and E. coli (7.26 and 3.28 mm), followed by ethanol extract (5.90 and 3.07 mm) and n-hexane extract (5.33 and 2.95 mm). The concentrations of 0.39 and 0.78% were the values of MIC and MBC for all three extracts, indicating that the extract of the calamus rhizome with several solvents in this study had the same antibacterial activity. 

scholarly journals A Facile Strategy to Fabricate Silver-Functionalized Superhydrophobic Cotton Fabrics with Long-Term Antibacterial Properties

2021 ◽  
Author(s):  
Zhanpeng Ye ◽  
Hui Rong ◽  
Shuangyang Li ◽  
Qing Ma ◽  
Liandong Deng ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Inhibition Zone ◽  
Dip Coating ◽  
Hybrid Nanoparticles ◽  
Preparation Process ◽  
E Coli ◽  
Wetting Properties ◽  
Medical Materials

Abstract Silver-functionalized textiles, have attracted considerable attention as the most common protective medical materials. However, their poor antibacterial durability and relatively tedious preparation process limit their applications. In this study, a kind of multifunctional cotton fabric was prepared through simultaneous dip-coating of silver-silica hybrid nanoparticles (Ag-MSNs) and fluorinated MSNs (F-MSNs), leading to excellent antifouling due to superhydrophobicity and long-term antibacterial properties from the sustained release of Ag ions (Ag+). Detailed studies were performed to evaluate their structure and protective performance, especially the long-term antibacterial properties of the obtained fabrics through the inhibition zone experiment for 25 d. The as-prepared fabrics showed good non-wetting properties and sustained antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). Further washing and abrasion experimentations demonstrated that the as-prepared fabrics had stable hydrophobicity and antibacterial properties, much more reliable than commercially derived antibacterial gauze. Considering the readily available inexpensive materials and simple preparation process as well as long-term and efficient sterilization effect, the strategy provided herein opens a new and facile way to fabricate multifunctional fabrics with robust hydrophobic and antibacterial activity for the future medical materials.

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