Synthesis, Characterization and Pharmacological Screening of Some Novel 2- substituted and 1(H)-substituted Benzimidazole Derivatives as potent Anti-cancer agents

The most of drugs containing Benzimidazole ring is a prominent structural motif found in numerous therapeutically active compounds. Benzimidazole and its synthetic analogues have been found to exhibit industrial, agricultural and biological application such as antitubercular, anti-inflammatory, analgesic, anticancer, anticoagulant, as well as good antifungal and anti microbial activity. Recent advances in technology considers microwave irradiation energy as the most efficient means of heating reactions for chemical transformations that can be accomplished in a minutes. Microwave irradiation assists organic synthesis (MAOS) not only helps in implementing green chemistry but also led to progress in organic synthesis. We report pharmacological screening of some novel 2 substituted and 1(h)-substituted Benzimidazole derivatives.

Pharmacological screening of Eryngium foetidum Linn – A Review

Eryngium foetidum L. (Family Apiaceae) is a biennial herb, and it is used as a culinary herb and spice across the different countries of the world, including Sri Lanka, India, Bangladesh, Malaysia, Singapore, etc. due to its high aroma quality. Also, it is used to treat several ailments, such as respiratory diseases, gastrointestinal ailments, and skin diseases among different indigenous populations for its medicinal properties. Based on ethnomedical evidence, many studies have been conducted to identify the phytoconstituents, underlying mechanisms, and related pharmacological effects of different parts of this plant. This study reviewed the current state of findings related to the Pharmacological activities of E. foetidum. Based on this review, this plant is widely used for ethnomedical and culinary purposes. Pharmacological screening of the plant revealed that it had different activities such as anti-inflammatory, antioxidant, antimicrobial, anthelminthic, anticonvulsant, anticancer, antidiabetic, antimalarial, larvicidal, and hepatoprotective activities. This review further promised that potential new chemical entities could be elicited from the phytoconstituents of E. foetidum.

Phenolic Compounds and Hepatoprotective Activity of Chicory Herb Extract

Introduction. Chicory (Cichorium intybus L.) is widely applied for liver disease treatment by traditional medicine of different countries; as well, it is the object for pharmacological research of hepatoprotective activity. In this regard, the method for obtaining dry extract of wild chicory herb (WCHE) is developed in the All-Russian Research Institute of Medicinal and Aromatic Plants.Aim. Aim of the research is determination of the qualitative composition of phenolic compounds, identification of the substances prevailing in WCHE and conducting pharmacological screening of the extract.Materials and methods. WCHE chemical composition has been explored with HPLC-MS/MS method; the main components were determined quantitatively with HPLC-UF method using single compounds that were isolated by us earlier and identified by NMR spectroscopy. WCHE pharmacological screening of hepatoprotective activity research was involving 50 male rats. Acute toxic hepatitis in animals was induced by a single subcutaneous injection of 50 % oily solution of tetrachloromethane (TCM) at a dosage of 0.4 ml per 100 g body weight. One hour before administration TCM, animals received WCHE at the doses of 100 or 500 mg/kg. 48 hours after TCM administration, the activity of serum enzymes alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), as well as the content of total bilirubin were determined for preliminary establishment of pharmacological activity. Pathomorphological studies of rat liver were carried out using histological methods. The liver histological structure was inspected using liver sections stained with hematoxylin and eosin.Results and discussion. The component composition of WCHE is represented by oxycoumarins, hydroxycinnamic acids and flavonoids. The dominant phenolic compounds are esculetin, chicoriin, chicoric, chlorogenic and caftaric acids. It was found under acute experimental toxic hepatitis, that preliminary WCHE administration reduces the toxic TCM effect on liver cells. In animals treated with WCHE at doses both 100 mg/kg and 500 mg/kg body weight, it was observed decreases in ALT activity by 35 % and 45 %, AST by 15 % and 28 %, alkaline phosphatase by 15 % and 21 %; the content of total bilirubin by 20 % and 29 %, respectively, in comparison with similar indicators in the group of animals that were not treated with the extract. The histological study showed that WCHE administration to animals at the doses of 100 and 500 mg/kg reduces dystrophic changes in hepatocytes, this effect is more pronounced at the extract dosage of 500 mg/kg.Conclusion. Main WCHE components are oxycoumarins (esculetin, chicoriin), hydroxycinnamic acids (chicoric, chlorogenic and caftaric). According to the results of screening studies, it was established that WCHE in doses of 100 mg/kg and 500 mg/kg is a promising object for further pharmacological research.

A novel Ayurvedic medical fumigant powder Bhootastgandha and its effect in Combating Airborne pathogens - A detailed pharmacological activity review

Infectious diseases are becoming popular with every passing day and viral infections among them are the cause for a large number of fatalities since last two decades. All the factors including the lifestyle pave the way to reduced immunity making the human population susceptible to infections. Management of diseases is not only important; prevention of further diseases should also be of prime concern. Infectious diseases that spread through a variety of mediums like air, water etc. requires preventive measures that controls the spread of the microorganisms and keep the preventive aspect intact. Ayurveda treatment has succeeded in achieving both treatment aspect as well as preventative aspect of diseases. Dhoopana Karma is an important modality & primitive method of sterilization which can be used in preventive and curative aspect. The yoga “Bhootastagandha” is taken from the Ayurvedic compilation Sarvaroga Chikitsa Ratnam”. Learning about the practical application of the formulation, the formulation is mentioned among a group of formulations used as fumigating agents. The formulation consists of a group of 8 drugs and all the drugs on pharmacological screening is found to possess Antimicrobial, Antibacterial, Antiviral, Antifungal properties among other pharmacological actions. The details about the pharmacological activity of the drugs are collected from various research articles published. This review article mainly focuses on the phytochemical properties of the ingredient drugs as per Ayurveda classics and the utility of the formulation as an effective fumigant keeping in mind the Antimicrobial, Anti-viral, Anti –Fungal and Anti- Bacterial action of the plants.

A REVIEW OF PHARMACOLOGICAL SCREENING METHODS FOR EXPERIMENTALLY INDUCED POLYCYSTIC OVARIAN DISEASE (PCOD)

PCOD is a complex heterogenous metabolic disorder affecting 10 % of women at their reproductive age. A well effective treatment for a complete cure for PCOD is still a challenge and evaluation of new strategies to treat this disease is of great priority. This review focuses on two aspects of PCOD, namely types of rodent screening models and diagnosis of major parameters. A fully convincing animal model to study PCOD is still a challenge as they are multi-ovular species. But in many ways, the rodent models are similar to human PCOD. This study explores on the parallels and problems associated with the use of different rodent models. Oligo- or anovulation, clinical or biochemical hyperandrogenism and polycystic ovaries constitute the key clinical features on which the diagnosis should be based. Determination of diagnosis parameters such as estrus cyclicity, insulin sensitivity, lipid profile, anti-oxidant status, weight of reproductive system and histopathological features of ovary are discussed along with the currently available treatment methods for PCOD.

Smilax zeylanica: A Promising Antilipidemix and Antiobesity Agent

Background: It is well established fact that obesity has now said to be a disease status.The phytochemicals have always been looked for their enormous stored potential. Similax zeylanica is a naturally occurring flavonoid known for its pharmacological potential. In this study it was aimed to determine the antiobesity and antilipidemic effect of various Similax zeylanica extract. Materials and Methods: The pharmacological screening of Cyclohexane extract of Smilax zeylanica (SZH), ethyl acetate extract Smilax zeylanica (SZE) and methanolic extract of Smilax zeylanica (SZM) was performed. The acute toxicity studies were performed on various animal groups. Obesity was diet induced.The plasma samples were investigated for various biochemical parameters such as glucose, lipid profile (Cholesterol, TG, HDL, LDL and VLDL), Liver function test (SGPT, SGOT). Results and Conclusion: The various parameters studied showed that the methanolic extracts of Similax zeylanica showed enormous antiobesity potential and concluded for the supplementation for antilipidemic effect.