scholarly journals Glyphosate Utilization as the Source of Carbon: Isolation and Identification of new Bacteria

2011 ◽  
Vol 8 (4) ◽  
pp. 1582-1587 ◽  
Author(s):  
M. Mohsen Nourouzi ◽  
T. G. Chuah ◽  
Thomas S. Y. Choong ◽  
C. J. Lim
Keyword(s):  
Carbon Source ◽  
Stenotrophomonas Maltophilia ◽  
Sole Carbon Source ◽  
Enrichment Culture ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Providencia Alcalifaciens ◽  
Mixed Bacteria ◽  
Aminomethyl Phosphonic Acid

Mixed bacteria from oil palm plantation soil (OPS) were isolated to investigate their ability to utilize glyphosate as carbon source. Results showed that approximately all of the glyphosate was converted to aminomethyl-phosphonic acid (AMPA) (99.5%). It is worthy to note that mixed bacteria were able to degrade only 2% of AMPA to further metabolites. Two bacterial strainsi.e. Stenotrophomonas maltophiliaandProvidencia alcalifacienswere obtained from enrichment culture. Bacterial isolates were cultured individually on glyphosate as a sole carbon source. It was observed that both isolates were able to convert glyphosate to AMPA.

scholarly journals Isolation and Characterization of Biosurfactant-producing Alcaligenes sp. YLA11 and its Diesel Degradation Potentials

2021 ◽  
Vol 9 (2) ◽  
pp. 7-12
Author(s):  
Abdulrahman Abdulhamid Arabo ◽  
Raji Arabi Bamanga ◽  
Mujiburrahman Fadilu ◽  
Musa Abubakar ◽  
Fatima Abdullahi Shehu ◽  
...  
Keyword(s):  
Carbon Source ◽  
Incubation Time ◽  
Sole Carbon Source ◽  
Enrichment Culture ◽  
Diesel Oil ◽  
Contaminated Site ◽  
Screening Methods ◽  
Bacterial Strains ◽  
Isolation And Characterization ◽  
Degrading Bacteria

This study aimed to isolate and identify biosurfactant producing and diesel alkanes degrading bacteria. For this reason, bacteria isolated from the diesel contaminated site were screened for their potential to produce biosurfactants and degrade diesel alkanes. Primary selection of diesel degraders was carried out by using conventional enrichment culture technique where 12 bacterial strains were isolated based on their ability to grow on minimal media supplemented with diesel as sole carbon source, which was followed by qualitative screening methods for potential biosurfactant production. Isolate B11 was the only candidate that shows positive signs for drop collapse, foaming, haemolytic test, oil displacement of more than 22 ± 0.05 mm, and emulsification (E24) of 14 ± 0.30%. The effect of various culture parameters (incubation time, diesel concentration, nitrogen source, pH and temperature) on biodegradation of diesel was evaluated. The optimum incubation time was confirmed to be 120 days for isolates B11, the optimum PH was confirmed as 8.0 for the isolate, Similarly, the optimum temperature was confirmed as 35oC. In addition, diesel oil was used as the sole carbon source for the isolates. The favourable diesel concentration was 12.5 % (v/v) for the isolate. The isolate has shown degradative ability towards Tridecane (C13), dodecane, 2, 6, 10-trimethyl- (C15), Tetradecane (C14), 2,6,10-Trimethyltridecane (C16), Pentadecane (C15). It degraded between 0.27% - 9.65% individual diesel oil alkanes. The strain has exhibited the potential of degrading diesel oil n-alkanes and was identified as Alcaligenes species strain B11 (MZ027604) using the 16S rRNA sequencing.

scholarly journals Effect of Bacterial Isolates From Soil Samples on Bisphenol A

2020 ◽  
Vol 31 (1) ◽  
pp. 15
Author(s):  
Dr.Neihaya Heikmat Zaki
Keyword(s):  
Carbon Source ◽  
Bisphenol A ◽  
Sole Carbon Source ◽  
Soil Samples ◽  
Triazine Ring ◽  
Bacterial Isolates ◽  
Optimal Temperature ◽  
Tigris River ◽  
End Products ◽  
Proteus Penneri

Twenty five samples were collected from the soil around the Tigris River from different locations in Iraqi cities, and 45 bacterial isolates were obtained. Three of these isolates were further tested for their degrading capacity of Bisphenol A (BPA) in Basal Mineral Medium, included: Pseudomonas orizohibtanis, Escherishia coli and Proteus penneri. The optimal temperature for the removal of BPA was determined at 20˚C, 37˚ and 45˚C for 1, 5, and 15 days, and the degradation increased up to a temperature of 37°C. Growth test was performed on isolated bacteria with BisPhenol A as the sole carbon source, and with increasing incubation time, the culture grew almost linearly to 24 hours. BPA decreased after 1days after incubating with tested bacterial isolates, and almost broken after 5 days, while it disappeared after 15 days at 37C, and Pseudomonas orizohibtanis exhibited the best degradation of BPA. The absorbance peaks in the UV region appeared at 222 and 276 nm and attributed to the benzene ring and triazine ring respectively. The end products of BPA degradation were analyzed by GCMS after 15 days of incubation. The chromatogram for Pseudomanas orizohibtanis showed three peaks at retention times of 70, 210 and 280 min, and referred to hexasiloxane, heptasiloxane, and Octasiloxane respectively. The present study was aimed to isolate bacteria from the soil of the Tigris River, and determined the ability to degrade Bisphenol-A, and characterized the environmental conditions of bacterial growth, and then analysis the products of the degradation by GC-MS.

scholarly journals Carbon source utilization and hydrogen production by isolated anaerobic bacteria

2016 ◽  
Vol 9 (1) ◽  
pp. 62-67 ◽  
Author(s):  
R. Jame ◽  
V. Zelená ◽  
B. Lakatoš ◽  
Ľ. Varečka
Keyword(s):  
Carbon Source ◽  
Anaerobic Fermentation ◽  
Carbon Sources ◽  
Anaerobic Bacteria ◽  
Growth Yield ◽  
Bacterial Isolates ◽  
Carbon Source Utilization ◽  
Bacterial Strains ◽  
Monocarboxylic Acids ◽  
Sheep Rumen

Abstract Five bacterial isolates were tested for their ability to generate hydrogen during anaerobic fermentation with various carbon sources. One isolate from sheep rumen was identified as Escherichia coli and four isolates belonged to Clostridium spp. Glucose, arabinose, ribose, xylose, lactose and cellobiose were used as carbon sources. Results showed that all bacterial strains could utilize these compounds, although the utilization of pentoses diminished growth yield. The excretion of monocarboxylic acids (acetate, propionate, formiate, butyrate) into medium was changed after replacing glucose by other carbon sources. Di- and tricarboxylic acids were excreted in negligible amounts only. Spectra of excreted carboxylic acids were unique for each strain and all carbon sources. All isolates produced H2 between 4—9 mmol·L−1 during the stationary phase of growth with glucose as energy source. This value was dramatically reduced when pentoses were used as carbon source. Lactose and cellobiose, starch and cellulose were suitable substrates for the H2 production in some but not all isolates. No H2 was produced by proteinaceous substrate, such as blood. Results show that both substrate utilization and physiological responses (growth, excretion of carboxylates, H2 production) are unique functions of each isolate.

scholarly journals Studies on poly-3-hydroxyoctanoate biosynthesis by a consortium of microorganisms

2016 ◽  
Vol 27 (1) ◽  
pp. 44-47 ◽  
Author(s):  
Mihaela Carmen Eremia ◽  
Irina Lupescu ◽  
Mariana Vladu ◽  
Maria Petrescu ◽  
Gabriela Savoiu ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Carbon Source ◽  
Pseudomonas Putida ◽  
Sole Carbon Source ◽  
Bacterial Biomass ◽  
Fermentation Medium ◽  
Energy Reserve ◽  
Bacterial Strains ◽  
Sodium Octanoate ◽  
Intracellular Energy

Abstract Polyhydroxyalcanoates (PHAs) are specifically produced by a wide variety of bacteria, as an intracellular energy reserve in the form of homo- and copolymers of [R]-β-hydroxyalkanoic acids, depending on the C source used for microorganism growth, when the cells are grown under stressing conditions. In this paper we present microbiological accumulation of poly-3-hydroxyoctanoate (PHO) by using a consortium of bacterial strains, Pseudomonas putida and Bacillus subtilis, in a rate of 3:1, grown on a fermentation medium based on sodium octanoate as the sole carbon source. The experiments performed in the above mentioned conditions led to the following results: from 18.70 g sodium octanoate (7.72 g/L in the fermentation medium) used up during the bioprocess, 3.93-3.96 g/L dry bacterial biomass and 1.834 - 1.884 g/L PHA, containing 85.83 - 86.8% PHO, were obtained.

The metabolism of p-fluorophenylacetic acid by a Pseudomonas sp. I. Isolation and identification of intermediates in degradation

1971 ◽  
Vol 17 (5) ◽  
pp. 635-644 ◽  
Author(s):  
D. B. Harper ◽  
E. R. Blakley
Keyword(s):  
Sole Carbon Source ◽  
Culture Medium ◽  
Enrichment Culture ◽  
Culture Technique ◽  
Spectroscopic Techniques ◽  
Resting Cells ◽  
Pseudomonas Sp ◽  
Isolation And Identification ◽  
Hydroxyphenylacetic Acid ◽  
Organic Fluorine

A Pseudomonas sp. capable of growing on p-fluorophenylacetic acid as sole carbon source has been isolated using the enrichment culture technique. All the organic fluorine is released into the culture medium as fluoride ion during growth. A number of fluorinated intermediates have been isolated from the culture medium when resting cells were incubated with the substrate. Using infrared, nuclear magnetic resonance, and mass spectroscopic techniques together with chemical degradative procedures, these have been identified as D(+)-monofluorosuccinic acid, trans-3-fluoro-3-hexenedioic acid, (−)-4-carboxymethyl-4-fluorobutanolide, 4-fluoro-2-hydroxyphenylacetic acid, and 4-fluoro-3-hydroxyphenylacetic acid.

UTILIZATION OF AROMATIC HYDROCARBONS BY ARTHROBACTER SPP.

1967 ◽  
Vol 13 (2) ◽  
pp. 205-211 ◽  
Author(s):  
I. L. Stevenson
Keyword(s):  
Carbon Source ◽  
Aromatic Hydrocarbons ◽  
Sole Carbon Source ◽  
Total Population ◽  
Residual Soil ◽  
Bacterial Isolates ◽  
Aromatic Substrates ◽  
Wide Range ◽  
Nutritional Studies ◽  
Different Soils

Bacterial isolates from a number of different soils were screened by growth observation and microscopic examination for Arthrobacter spp. Incidence of arthrobacter in the total population varied, but averaged around 15% in the soils investigated. One hundred and thirty arthrobacter isolates were tested for their ability to utilize aromatic hydrocarbons as their sole carbon source. Seventy-seven percent of these organisms were able to grow on at least two aromatic substrates and many were capable of growth on a wide range of these compounds. Nutritional studies indicated that arthrobacter with simple requirements were able to utilize the greatest number of aromatic hydrocarbons as their sole carbon source. The ability of the arthrobacter to metabolize aromatic compounds is discussed in terms of their possible role in the formation and turnover of residual soil organic matter.

scholarly journals Isolation and Potential Testing of North Sumatera Berastagi Agricultural Soil In Degrading Marshal Insecticide With Carbosulfan Active Material

2020 ◽  
Vol 2 (02) ◽  
pp. 147-156
Author(s):  
Nunuk Priyani
Keyword(s):  
Carbon Source ◽  
Agricultural Soil ◽  
Sole Carbon Source ◽  
Active Material ◽  
Bacterial Isolates ◽  
Selective Media

The isolation of bacteria from Berastagi agricultural soil North Sumatera has been done. The aim is to evaluate their ability in degrading carbosulfan. Sixteen bacterial isolates were obtained using selective media Bushnel Hass Agar (BHA) containing 12 ppm of carbosulfan. The parameters observed were the growth of isolates, biosurfactant activity, biosurfactant concentration, and the residue of carbosulfan after 21 days of incubation. The result showed that all isolates were able to degrade carbosulfan as the sole carbon source. Two isolates namely JBM 3 (isolate from citrus agricultural soil Berastagi) and KBM 1 (isolate from cabbage agricultural soil Berastagi) were selected for further test to determine their ability to degrade carbosulfan. The results showed that both of the isolates were able to degrade carbosulfan. Compare to control, isolate JBM 3 was able to decrease the concentration of carbosulfan by 33.33%, while isolate KBM 1 was able to reduce carbosulfan concentration up to 40.47%.

scholarly journals Combining Culture-Dependent and Independent Approaches for the Optimization of Epoxiconazole and Fludioxonil-Degrading Bacterial Consortia

2021 ◽  
Vol 9 (10) ◽  
pp. 2109
Author(s):  
Diogo Alexandrino ◽  
Ana Mucha ◽  
Maria Paola Tomasino ◽  
C. Marisa R. Almeida ◽  
Maria Carvalho
Keyword(s):  
Agricultural Soil ◽  
Enrichment Culture ◽  
Bacterial Species ◽  
The Other ◽  
Microbial Consortia ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Bacterial Consortia ◽  
First Time ◽  
Culture Dependent

Epoxiconazole (EPO) and fludioxonil (FLU) are two widely used fluorinated pesticides known to be highly persistent and with high ecotoxicological potential, turning them into pollutants of concern. This work aimed to optimize two degrading bacterial consortia, previously obtained from an agricultural soil through enrichment with EPO and FLU, by characterizing the contribution of their corresponding bacterial isolates to the biodegradation of these pesticides using both culture-dependent and independent methodologies. Results showed that a co-culture of the strains Hydrogenophaga eletricum 5AE and Methylobacillus sp. 8AE was the most efficient in biodegrading EPO, being able to defluorinate ca. 80% of this pesticide in 28 days. This catabolic performance is likely the result of a commensalistic cooperation, in which H. eletricum may be the defluorinating strain and Methylobacillus sp. may assume an accessory, yet pivotal, catabolic role. Furthermore, 16S rRNA metabarcoding analysis revealed that these strains represent a minority in their original consortium, showing that the biodegradation of EPO can be driven by less abundant phylotypes in the community. On the other hand, none of the tested combinations of bacterial strains showed potential to biodegrade FLU, indicating that the key degrading strains were not successfully isolated from the original enrichment culture. Overall, this work shows, for the first time, the direct involvement of two bacterial species, namely H. eletricum and Methylobacillus sp., in the biodegradation of EPO, while also offering insight on how they might cooperate to accomplish this process. Moreover, the importance of adequate culture-dependent approaches in the engineering of microbial consortia for bioremediation purposes is also emphasized.

scholarly journals Production of Polyhydroxyalkanoates (PHA) for Biomedical Application using Sugar Industry Waste and Native Micro Flora via Biphasic System.

2019 ◽  
Vol 7 (4.14) ◽  
pp. 7
Author(s):  
P Paulraj ◽  
S Periyavedi ◽  
K Sajna ◽  
P T Mohamed Javad ◽  
P Sajeesh ◽  
...  
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
Biomedical Application ◽  
Sugar Industry ◽  
Cellulolytic Activity ◽  
Bacterial Strains ◽  
Industry Waste ◽  
Research Production ◽  
System A ◽  
The Cost

Plastics are synthetic polymers that changed our daily life due to their applications. But it’s increasing environmental problems had made the researchers find the alternative way such as usage of biodegradable plastics like Polyhydroxyalkanoates (PHA) which has similar properties to the conventional plastic. In this research production and characterization of PHAs using sugar industry wastes as a sole carbon source was carried in order to reduce the cost of production of PHA. Isolation of heterotrophic native micro flora from soil sample contaminated with sugar effluent for amylolytic and cellulolytic activity with PHA production potentials was carried out.  Suitable conditions for PHA accumulation were optimized with 1X and 2X MSM with sugar effluent as a sole carbon source and bacteriostatic antibiotics as an inducer using bi-phasic system. A total of 14 different heterotrophic native bacterial strains were isolated, among them, 4 isolates showed starch hydrolytic property, 7 isolates showed cellulolytic activity and 7 were PHA producers. The strain with the highest PHA accumulation (99.99µg/mL of culture) in 2X MSM with sugar effluent within 6 hours was considered as a potential strain (53% of PHB CWD). The isolate was confirmed as Chryseobacterium Sp. using 16S rRNA sequencing. 

scholarly journals Biodegradation of Bis(2-Chloroethyl) Ether by Xanthobacter sp. Strain ENV481

2007 ◽  
Vol 73 (21) ◽  
pp. 6870-6875 ◽  
Author(s):  
Kevin McClay ◽  
Charles E. Schaefer ◽  
Simon Vainberg ◽  
Robert J. Steffan
Keyword(s):  
United States ◽  
Carbon Source ◽  
Diethylene Glycol ◽  
Sole Carbon Source ◽  
Bacterial Isolate ◽  
Chloroacetic Acid ◽  
Sole Source ◽  
Superfund Site ◽  
Bacterial Strains ◽  
Northeastern United States

ABSTRACT Degradation of bis(2-chloroethyl) ether (BCEE) was observed to occur in two bacterial strains. Strain ENV481, a Xanthobacter sp. strain, was isolated by enrichment culturing of samples from a Superfund site located in the northeastern United States. The strain was able to grow on BCEE or 2-chloroethylethyl ether as the sole source of carbon and energy. BCEE degradation in strain ENV481 was facilitated by sequential dehalogenation reactions resulting in the formation of 2-(2-chloroethoxy)ethanol and diethylene glycol (DEG), respectively. 2-Hydroxyethoxyacetic acid was detected as a product of DEG catabolism by the strain. Degradation of BCEE by strain ENV481 was independent of oxygen, and the strain was not able to grow on a mixture of benzene, ethylbenzene, toluene, and xylenes, other prevalent contaminants at the site. Another bacterial isolate, Pseudonocardia sp. strain ENV478 (S. Vainberg et al., Appl. Environ. Microbiol. 72:5218-5224, 2006), degraded BCEE after growth on tetrahydrofuran or propane but was not able to grow on BCEE as a sole carbon source. BCEE degradation by strain ENV478 appeared to be facilitated by a monooxygenase-mediated O-dealkylation mechanism, and it resulted in the accumulation of 2-chloroacetic acid that was not readily degraded by the strain.

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