scholarly journals Combining Culture-Dependent and Independent Approaches for the Optimization of Epoxiconazole and Fludioxonil-Degrading Bacterial Consortia

2021 ◽  
Vol 9 (10) ◽  
pp. 2109
Author(s):  
Diogo Alexandrino ◽  
Ana Mucha ◽  
Maria Paola Tomasino ◽  
C. Marisa R. Almeida ◽  
Maria Carvalho
Keyword(s):  
Agricultural Soil ◽  
Enrichment Culture ◽  
Bacterial Species ◽  
The Other ◽  
Microbial Consortia ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Bacterial Consortia ◽  
First Time ◽  
Culture Dependent

Epoxiconazole (EPO) and fludioxonil (FLU) are two widely used fluorinated pesticides known to be highly persistent and with high ecotoxicological potential, turning them into pollutants of concern. This work aimed to optimize two degrading bacterial consortia, previously obtained from an agricultural soil through enrichment with EPO and FLU, by characterizing the contribution of their corresponding bacterial isolates to the biodegradation of these pesticides using both culture-dependent and independent methodologies. Results showed that a co-culture of the strains Hydrogenophaga eletricum 5AE and Methylobacillus sp. 8AE was the most efficient in biodegrading EPO, being able to defluorinate ca. 80% of this pesticide in 28 days. This catabolic performance is likely the result of a commensalistic cooperation, in which H. eletricum may be the defluorinating strain and Methylobacillus sp. may assume an accessory, yet pivotal, catabolic role. Furthermore, 16S rRNA metabarcoding analysis revealed that these strains represent a minority in their original consortium, showing that the biodegradation of EPO can be driven by less abundant phylotypes in the community. On the other hand, none of the tested combinations of bacterial strains showed potential to biodegrade FLU, indicating that the key degrading strains were not successfully isolated from the original enrichment culture. Overall, this work shows, for the first time, the direct involvement of two bacterial species, namely H. eletricum and Methylobacillus sp., in the biodegradation of EPO, while also offering insight on how they might cooperate to accomplish this process. Moreover, the importance of adequate culture-dependent approaches in the engineering of microbial consortia for bioremediation purposes is also emphasized.

scholarly journals Temporal Stability and Biodiversity of Two Complex Antilisterial Cheese-Ripening Microbial Consortia

2003 ◽  
Vol 69 (7) ◽  
pp. 4012-4018 ◽  
Author(s):  
Ariel Maoz ◽  
Ralf Mayr ◽  
Siegfried Scherer
Keyword(s):  
Species Composition ◽  
Bacterial Species ◽  
Temporal Stability ◽  
Individual Characteristics ◽  
Microbial Consortia ◽  
Cheese Ripening ◽  
Safe Food ◽  
The Stability ◽  
The Individual ◽  
First Time

ABSTRACT The temporal stability and diversity of bacterial species composition as well as the antilisterial potential of two different, complex, and undefined microbial consortia from red-smear soft cheeses were investigated. Samples were collected twice, at 6-month intervals, from each of two food producers, and a total of 400 bacterial isolates were identified by Fourier-transform infrared spectroscopy and 16S ribosomal DNA sequence analysis. Coryneform bacteria represented the majority of the isolates, with certain species being predominant. In addition, Marinolactobacillus psychrotolerans, Halomonas venusta, Halomonas variabilis, Halomonas sp. (106 to 107 CFU per g of smear), and an unknown, gram-positive bacterium (107 to 108 CFU per g of smear) are described for the first time in such a consortium. The species composition of one consortium was quite stable over 6 months, but the other consortium revealed less diversity of coryneform species as well as less stability. While the first consortium had a stable, extraordinarily high antilisterial potential in situ, the antilisterial activity of the second consortium was lower and decreased with time. The cause for the antilisterial activity of the two consortia remained unknown but is not due to the secretion of soluble, inhibitory substances by the individual components of the consortium. Our data indicate that the stability over time and a potential antilisterial activity are individual characteristics of the ripening consortia which can be monitored and used for safe food production without artificial preservatives.

Isolation and Genetic Identification of Dibenzothiophene Degrading Bacteria from Contaminated Soil

2012 ◽  
Vol 610-613 ◽  
pp. 292-295 ◽  
Author(s):  
Lin Li ◽  
Chao Cheng Zhao ◽  
Qi You Liu ◽  
Yun Bo Zhang
Keyword(s):  
Phylogenetic Analysis ◽  
Species Identification ◽  
Contaminated Soil ◽  
Bacterial Strain ◽  
Degradation Efficiency ◽  
Genetic Identification ◽  
Microbial Consortia ◽  
Bacterial Strains ◽  
Bacterial Consortia ◽  
Degrading Bacteria

The biodegradation abilities of 10 dibenzothiophene degrading microbial consortia isolated from contaminated soil were investigated. 5 highly efficient dibenzothiophene degrading bacterial strains were obtained from the consortium LKY10 by screening on LB-agar plates.The bacterial strain LKY10-5 reduced more than 90% of dibenzothiophene with 40 mg•L-1concentration, and had higher degradation efficiency than enriched bacterial consortia in 7 days of cultivation. According to species identification and phylogenetic analysis, strain LKY10-1 and LKY10-3 belonged to Actinobacteria and could be included in Rhodococcus and Cellulosimicrobium genus, LKY10-5 and LKY10-6 belonged to Proteobacteria and could be included in Pseudomonas and Devosia genus, and LKY10-13 could be included in Lysinibacillus genus and belonged to Firmicutes.

scholarly journals Atypical Aeromonas salmonicida vapA type V and Vibrio spp. are predominant bacteria recovered from ballan wrasse Labrus bergylta in Scotland

2020 ◽  
Vol 140 ◽  
pp. 47-54 ◽  
Author(s):  
A Papadopoulou ◽  
T Wallis ◽  
JG Ramirez-Paredes ◽  
SJ Monaghan ◽  
A Davie ◽  
...  
Keyword(s):  
Bacterial Species ◽  
Skin Lesions ◽  
Aeromonas Salmonicida ◽  
Sequencing Analysis ◽  
Bacterial Isolates ◽  
Positive Individual ◽  
Labrus Bergylta ◽  
Type V ◽  
First Time ◽  
Vibrio Spp

Healthy and/or moribund farmed and wild ballan wrasse Labrus bergylta (>0.5 to 900 g) were sampled from hatcheries (n = 2) and Atlantic salmon Salmo salar cage sites (n = 8) in Scotland between February 2016 and October 2018. Less than half of the sampled individuals (n = 43; 32.3%) had been vaccinated (autogenous polyvalent vaccine; dip and/or injection) against atypical furunculosis (type V and VI), while 20 (15.0%) fish were not vaccinated, and the rest (70 individuals, 52.7%) were of unknown vaccination status. Swab samples from skin lesions, gill, liver, spleen and kidney were inoculated onto a variety of bacteriological agar plates, and bacteriology identification and sequencing analysis was performed on significant bacterial colonies. Atypical Aeromonas salmonicida (aAs) vapA type V was the predominant bacterial species (70/215 bacterial isolates, 32.5% of bacterial samples; 43/117 positive individual fish, 36.8%) isolated in this survey followed by Vibrio species, which were the most geographically prevalent bacteria. Photobacterium indicum/profundum was also isolated from L. bergylta for the first time during this study. The collection of these bacterial isolates provides useful information for disease management. Identifying the aAs isolates involved in disease in ballan wrasse could provide vital information for improving/updating existing autogenous vaccines.

scholarly journals β-Lactamase inhibition profile of new amidine-substituted diazabicyclooctanes

2021 ◽  
Vol 17 ◽  
pp. 711-718
Author(s):  
Zafar Iqbal ◽  
Lijuan Zhai ◽  
Yuanyu Gao ◽  
Dong Tang ◽  
Xueqin Ma ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Second Generation ◽  
Bacterial Species ◽  
The Other ◽  
Inhibition Activity ◽  
Bacterial Strains ◽  
E Coli ◽  
Mic Value

The diazabicyclooctane (DBO) scaffold is the backbone of non-β-lactam-based second generation β-lactamase inhibitors. As part of our efforts, we have synthesized a series of DBO derivatives A1–23 containing amidine substituents at the C2 position of the bicyclic ring. These compounds, alone and in combination with meropenem, were tested against ten bacterial strains for their antibacterial activity in vitro. All compounds did not show antibacterial activity when tested alone (MIC >64 mg/L), however, they exhibited a moderate inhibition activity in the presence of meropenem by lowering its MIC values. The compound A12 proved most potent among the other counterparts against all bacterial species with MIC from <0.125 mg/L to 2 mg/L, and is comparable to avibactam against both E. coli strains with a MIC value of <0.125 mg/L.

scholarly journals Establishing and Optimizing a Bacterial Consortia for Effective Biodegradation of Petroleum Contaminants: Advancing Classical Microbiology via Experimental and Mathematical Approach

Water ◽  
2021 ◽  
Vol 13 (22) ◽  
pp. 3311
Author(s):  
Baichun Wu ◽  
Jingmin Deng ◽  
Hao Niu ◽  
Jiahao Liang ◽  
Muhammad Arslan ◽  
...  
Keyword(s):  
Partial Correlation ◽  
High Efficiency ◽  
Bacterial Species ◽  
Rhodococcus Erythropolis ◽  
Bacterial Consortium ◽  
Bacterial Strains ◽  
Bacterial Consortia ◽  
Orthogonal Experiments ◽  
Petroleum Contaminants ◽  
Different Strains

In classical microbiology, developing a high-efficiency bacterial consortium is a great challenge for faster biodegradation of petroleum contaminants. In this study, a systematic experimental and mathematical procedure was adopted to establish a bacterial consortium for the effective biodegradation of heavy oil constituents. A total of 27 bacterial consortia were established as per orthogonal experiments, using 8 petroleum-degrading bacterial strains. These bacteria were closer phylogenetic relatives of Brevundimonas sp. Tibet-IX23 (Y1), Bacillus firmus YHSA15, B. cereus MTCC 9817, B. aquimaris AT8 (Y2, Y6 and Y7), Pseudomonas alcaligenes NBRC (Y3), Microbacterium oxydans CV8.4 (Y4), Rhodococcus erythropolis SBUG 2052 (Y5), and Planococcus sp. Tibet-IX21 (Y8), and were used in different combinations. Partial correlation analysis and a general linear model hereafter were applied to investigate interspecific relationships among different strains and consortia. The Y1 bacterial species showed a remarkable synergy, whereas Y3, Y4, and Y6 displayed a strong antagonism in all consortia. Inoculation ratios of different strains significantly influenced biodegradation. An optimal consortium was constructed with Y1, Y2, Y5, Y7, and Y8, which revealed maximum degradation of 11.238 mg/mL OD600 for oil contaminants. This study provides a line of evidence that a functional consortium can be established by mathematical models for improved bioremediation of petroleum-contaminated environment.

scholarly journals Engineered Interspecies Amino Acid Cross-Feeding Increases Population Evenness in a Synthetic Bacterial Consortium

mSystems ◽  
2019 ◽  
Vol 4 (4) ◽  
Author(s):  
Marika Ziesack ◽  
Travis Gibson ◽  
John K. W. Oliver ◽  
Andrew M. Shumaker ◽  
Bryan B. Hsu ◽  
...  
Keyword(s):  
Bacterial Species ◽  
Bacterial Consortium ◽  
Microbial Interactions ◽  
Environmental Benefits ◽  
Microbial Consortia ◽  
Positive Interactions ◽  
Complex Environments ◽  
Bacterial Consortia ◽  
Health And Disease ◽  
Antagonistic Interactions

ABSTRACT In nature, microbes interact antagonistically, neutrally, or beneficially. To shed light on the effects of positive interactions in microbial consortia, we introduced metabolic dependencies and metabolite overproduction into four bacterial species. While antagonistic interactions govern the wild-type consortium behavior, the genetic modifications alleviated antagonistic interactions and resulted in beneficial interactions. Engineered cross-feeding increased population evenness, a component of ecological diversity, in different environments, including in a more complex gnotobiotic mouse gut environment. Our findings suggest that metabolite cross-feeding could be used as a tool for intentionally shaping microbial consortia in complex environments. IMPORTANCE Microbial communities are ubiquitous in nature. Bacterial consortia live in and on our body and in our environment, and more recently, biotechnology is applying microbial consortia for bioproduction. As part of our body, bacterial consortia influence us in health and disease. Microbial consortium function is determined by its composition, which in turn is driven by the interactions between species. Further understanding of microbial interactions will help us in deciphering how consortia function in complex environments and may enable us to modify microbial consortia for health and environmental benefits.

scholarly journals Characterization of gtf, a Glucosyltransferase Gene in the Genomes of Pediococcus parvulus and Oenococcus oeni, Two Bacterial Species Commonly Found in Wine

2008 ◽  
Vol 74 (13) ◽  
pp. 4079-4090 ◽  
Author(s):  
Marguerite Dols-Lafargue ◽  
Hyo Young Lee ◽  
Claire Le Marrec ◽  
Alain Heyraud ◽  
Gérard Chambat ◽  
...  
Keyword(s):  
Bacterial Species ◽  
Oenococcus Oeni ◽  
Mobile Elements ◽  
The Other ◽  
Bacterial Strains ◽  
Model Medium ◽  
Polysaccharide Synthesis

ABSTRACT “Ropiness” is a bacterial alteration in wines, beers, and ciders, caused by β-glucan-synthesizing pediococci. A single glucosyltransferase, Gtf, controls ropy polysaccharide synthesis. In this study, we show that the corresponding gtf gene is also present on the chromosomes of several strains of Oenococcus oeni isolated from nonropy wines. gtf is surrounded by mobile elements that may be implicated in its integration into the chromosome of O. oeni. gtf is expressed in all the gtf + strains, and β-glucan is detected in the majority of these strains. Part of this β-glucan accumulates around the cells forming a capsule, while the other part is liberated into the medium together with heteropolysaccharides. Most of the time, this polymer excretion does not lead to ropiness in a model medium. In addition, we show that wild or recombinant bacterial strains harboring a functional gtf gene (gtf +) are more resistant to several stresses occurring in wine (alcohol, pH, and SO2) and exhibit increased adhesion capacities compared to their gtf mutant variants.

scholarly journals Study the antibiotics sensitivity and beta- lactamase productivity of some Staphylococcus spp. Isolates from different sources of the Al Jamhoree Teaching Hospital in Mosul City

2019 ◽  
Vol 24 (7) ◽  
pp. 27
Author(s):  
Ayman Mohammed Khaleel1 ◽  
Adeeba Younis Shareef ◽  
, Shakeeba Younis Shareef
Keyword(s):  
Staphylococcus Aureus ◽  
Teaching Hospital ◽  
Clinical Laboratory ◽  
Bacterial Species ◽  
Age Groups ◽  
National Committee ◽  
Beta Lactamase ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Staphylococcus Spp

The study includes the isolation of certain types of Gram-positive bacteria Staphylococcus spp..The sample materials (blood, wounds, burns) were collected from both genders of all age groups of inpatients in Al Jamhoree Teaching Hospital in the Mosul during June till end of November 2018. Staphylococcus aureus was the most common among the isolates with 7 isolates(38.9%) followed by Staphylococcus epidermidis, Staphylococcus scriuri Staphylococcus lentus (Staphylococcus simulans) with 2 isolates each with 11.1% followed by Staphylococcus chromogenase, Staphylococcu scapilis and  Staphylococcus xylosus with one isolate for each one 5.5%. The number of isolates was 9 isolates and 50%, followed by wound samples. The isolates were 6 isolated by 33% and the samples of burns were 3 isolates. 17% were isolated and Staphylococcus aureus was the most dominant species. The sensitivity of bacterial isolates was  studied for 15 antibiotics for different antibiotic groups. The results showed that a difference in the resistance ratio of the isolates to these antagonists, as they were resistant to the Ceftrixone, Cloxacillin 100%. The Ciprofloxacillin, Gentamycin, Pipracillin, and Amikacin resistance were  reduced. The Ciprofloxacillin antagonist was the most affected on the bacterial isolates studied, followed by the Rifampin. The results showed that the beta-lactamase enzyme was not produced by any of the Iodic methods by 4 bacterial strains, while the rest of the species varied in the susceptibility of production to the enzyme. The Iodine tube is one of the best methods to detect the production of these enzymes. The susceptibility of bacterial species to the production of large-spectrum beta-lactase enzymes was also tested using the National Committee for Clinical Laboratory Standards (NCCL) and the double-disc method. Staphylococcus lentus, Staphylococcus capilis, Staphylococcus chromanogenes The first NCCL has shown a single isolation of Staphylococcus capilis ability to produce it.   http://dx.doi.org/10.25130/tjps.24.2019.125

scholarly journals Screening and characterisation of bioplastics producing bacteria isolated from oil contaminated soils of Virudhunagar, Tamil Nadu, India

2017 ◽  
Vol 4 (1) ◽  
pp. 21
Author(s):  
K. Susithra ◽  
U. Ramesh ◽  
M. Kannan ◽  
R. Ganesan ◽  
K. Rajarathinam
Keyword(s):  
Contaminated Soils ◽  
Tamil Nadu ◽  
Bacterial Species ◽  
Carbon Sources ◽  
Drug Carriers ◽  
High Yield ◽  
Potential Candidate ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Phb Production

Objective: To screen the high yielding PHB producing bacterial strains and its characterization by phenotypic methods and PHB production was optimized by using different carbon sources, different levels of pH and temperature.Methods: The oil contaminated soils of Virudhunagar town were chosen for the isolation of PHB producing bacterial species. The bacterial isolates were stained by Sudan Black B method for observing the presence of PHB granules and further confirmation by UV and FTIR analysis. The PHB accumulations in bacterial isolates were done by UV-Spectrophotometric analysis. The maximum PHB production was optimized by varying pH, temperature and carbon sources used in the production medium.Results: From the microscopic observations of the individual isolates, seven bacterial isolates were seen by the PHB granules inside the cell. The UV results interpret maximum amount 1052 microgram/ml of PHB by the second bacterial isolate. FTIR confirmed C=O group in the extracted PHB from production media. Also high yield was observed in at pH 9, 370 C and starch.Conclusions: These PHB have potential candidate for some application in packaging and biomedical material production in the form of drug carriers.

Identification of Dominant Bacteria Isolated From Periodontal Abscesses

2021 ◽  
pp. 232020682110507
Author(s):  
Kubra Karacam ◽  
Turgut Demir ◽  
Ozlem Baris
Keyword(s):  
Bacterial Species ◽  
The Body ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Rdna Sequencing ◽  
Bacterial Content ◽  
Dominant Bacteria ◽  
Research Criteria ◽  
First Time ◽  
Culture Dependent

Aim: Various methods investigating the bacterial content causing periodontal abscesses have been applied in studies conducted until today. However, these studies have focused on periodontopathogens. Our study was carried out to research whether different pathogens other than the known periodontopathogens are present in periodontal abscess formation. Therefore, dominant bacterial samples obtained from the periodontal abscess content using the culture-dependent method were identified by 16S rDNA sequencing. Materials and Methods: Samples were obtained using a syringe or a periopaper from periodontal abscesses of 20 volunteers who met the research criteria. The three different bacterial colonies that were observed most intensely in each sample were selected and purified, and the isolates obtained were kept until the next characterization. Genomic DNA was isolated from each isolate; 16S rRNA genes were amplified by polymerase chain reaction and identified using DNA sequencing analyses. Results: As a result of culture-dependent methods, bacterial species belonging to Streptococcus, Staphylococcus, Neisseria, Actinomyces, Morococcus, Moraxella, and Enterococcus genera were isolated from a total of 60 bacterial isolates, three of which were the most densely growing colonies from each periodontal abscess sample. Conclusion: In our study, most of the bacterial species detected were identified for the first time in the bacterial content of periodontal abscesses. In some previously done studies, most of these bacteria species were shown to cause abscesses in different parts of the body. It was concluded that further studies are needed to determine the number and proportion of these bacteria species in total bacterial content to evaluate whether they cause periodontal abscesses.

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