The metabolism of p-fluorophenylacetic acid by a Pseudomonas sp. I. Isolation and identification of intermediates in degradation

1971 ◽  
Vol 17 (5) ◽  
pp. 635-644 ◽  
Author(s):  
D. B. Harper ◽  
E. R. Blakley
Keyword(s):  
Sole Carbon Source ◽  
Culture Medium ◽  
Enrichment Culture ◽  
Culture Technique ◽  
Spectroscopic Techniques ◽  
Resting Cells ◽  
Pseudomonas Sp ◽  
Isolation And Identification ◽  
Hydroxyphenylacetic Acid ◽  
Organic Fluorine

A Pseudomonas sp. capable of growing on p-fluorophenylacetic acid as sole carbon source has been isolated using the enrichment culture technique. All the organic fluorine is released into the culture medium as fluoride ion during growth. A number of fluorinated intermediates have been isolated from the culture medium when resting cells were incubated with the substrate. Using infrared, nuclear magnetic resonance, and mass spectroscopic techniques together with chemical degradative procedures, these have been identified as D(+)-monofluorosuccinic acid, trans-3-fluoro-3-hexenedioic acid, (−)-4-carboxymethyl-4-fluorobutanolide, 4-fluoro-2-hydroxyphenylacetic acid, and 4-fluoro-3-hydroxyphenylacetic acid.

The metabolism of p-fluorobenzoic acid by a Pseudomonas sp.

1971 ◽  
Vol 17 (8) ◽  
pp. 1015-1023 ◽  
Author(s):  
D. B. Harper ◽  
E. R. Blakley
Keyword(s):  
Metabolic Pathways ◽  
Culture Medium ◽  
Resting Cells ◽  
Pseudomonas Sp ◽  
Metabolic Intermediates ◽  
Organic Fluorine

A species of Pseudomonas previously used to study the degradation of p-fluorophenylacetic acid was used to investigate the degradation of p-fluorobenzoic acid. During growth on the latter substrate all organic fluorine was released into the culture medium as fluoride. Several metabolic intermediates were isolated from the culture medium of resting cells. The major compounds have been identified as 4-fluorocatechol, β-acetylacrylic acid, (+)-4-carboxymethyl-4-fiuorobut-2-enolide, (−)-4-carboxymethyl-but-2-enolide, and β-ketoadipic acid. A small quantity of a compound tentatively identified as β-fiuoroacrylic acid was also isolated. On the basis of these findings, together with respiratory studies on p-fluorobenzoic acid grown cells with various substrates, two metabolic pathways are proposed which involve elimination of fluorine at either of two alternative stages in the breakdown of p-fluorobenzoic acid.

scholarly journals Chemical Constituents of Cichorium intybus and their Inhibitory Effects against Urease and α-Chymotrypsin Enzymes

2011 ◽  
Vol 6 (8) ◽  
pp. 1934578X1100600
Author(s):  
Sumayya Saied ◽  
Shazia Shah ◽  
Zulfiqar Ali ◽  
Ajmal Khan ◽  
Bishnu P. Marasini ◽  
...  
Keyword(s):  
Chemical Constituents ◽  
2D Nmr ◽  
Cichorium Intybus ◽  
Spectroscopic Techniques ◽  
Inhibitory Effects ◽  
Isolation And Identification ◽  
Aerial Parts ◽  
Phytochemical Investigation ◽  
Hydroxyphenylacetic Acid ◽  
First Time

Phytochemical investigation of the aerial parts of Cichorium intybus L. resulted in the isolation and identification of two new natural metabolites, 2,6-di[but-3( E)-en-2-onyl]naphthalene (1), and 3,3′,4,4′-tetrahydroxychalcone (2), along with nine known compounds. Their structures were determined by spectroscopic techniques including 1D and 2D NMR. The known compounds were identified as scopoletin (3), 4-hydroxyphenylacetic acid (4), 3-hydroxy-4-methoxybenzoic acid (5), 4,4′-dihydroxychalcone (6), 6,7-dihydroxycoumarine (7), 1-triacontanol (8), lupeol (9), β-sitosterol (10), and β-sitosterol-3- O-β-glucopyranoside (11). Compounds 4-6 and 8 are reported for the first time from C. intybus. Compounds 2 and 3 showed weak inhibitory activities against urease and α-chymotrypsin enzymes, respectively.

scholarly journals Glyphosate Utilization as the Source of Carbon: Isolation and Identification of new Bacteria

2011 ◽  
Vol 8 (4) ◽  
pp. 1582-1587 ◽  
Author(s):  
M. Mohsen Nourouzi ◽  
T. G. Chuah ◽  
Thomas S. Y. Choong ◽  
C. J. Lim
Keyword(s):  
Carbon Source ◽  
Stenotrophomonas Maltophilia ◽  
Sole Carbon Source ◽  
Enrichment Culture ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Providencia Alcalifaciens ◽  
Mixed Bacteria ◽  
Aminomethyl Phosphonic Acid

Mixed bacteria from oil palm plantation soil (OPS) were isolated to investigate their ability to utilize glyphosate as carbon source. Results showed that approximately all of the glyphosate was converted to aminomethyl-phosphonic acid (AMPA) (99.5%). It is worthy to note that mixed bacteria were able to degrade only 2% of AMPA to further metabolites. Two bacterial strainsi.e. Stenotrophomonas maltophiliaandProvidencia alcalifacienswere obtained from enrichment culture. Bacterial isolates were cultured individually on glyphosate as a sole carbon source. It was observed that both isolates were able to convert glyphosate to AMPA.

scholarly journals Biodegradation of Eugenol byBacillus CereusStrain PN24

2010 ◽  
Vol 7 (s1) ◽  
pp. S474-S480 ◽  
Author(s):  
Jagannath C. Kadakol ◽  
Chandrappa M. Kamanavalli
Keyword(s):  
Vanillic Acid ◽  
Bacterial Strain ◽  
Culture Medium ◽  
Protocatechuic Acid ◽  
Enrichment Culture ◽  
Culture Method ◽  
Sole Source ◽  
The Other ◽  
Cell Free Extract ◽  
Isolation And Identification

Bacillus cereusPN24 was isolated from soil by a conventional enrichment culture method using eugenol as a sole source of carbon and energy. The organism also utilized eugenol, 4-vinyl guaiacol, vanillin, vanillic acid and protocatechuic acid as growth substrates. The organism degraded eugenol to protocatechuic acid, which was further metabolized by aβ-ketoadipate pathway. On the other hand, the intermediate of the eugenol-degrading pathway, such as ferulic acid was not detected in the culture medium as an intermediate, as evidenced by isolation and identification of metabolites and enzyme activities in the cell-free extract. Such a bacterial strain could be used for phenolic environmental clean-up given optimal nutrient conditions.

scholarly journals Characterizations of novel pesticide-degrading bacterial strains from industrial wastes found in the industrial cities of Pakistan and their biodegradation potential

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12211
Author(s):  
Noreen Asim ◽  
Mahreen Hassan ◽  
Farheen Shafique ◽  
Maham Ali ◽  
Hina Nayab ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Enrichment Culture ◽  
Culture Technique ◽  
Industrial Wastes ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
16S Rrna Analysis ◽  
Degrading Bacteria ◽  
Considerable Degradation ◽  
Different Strains

Background Lack of infrastructure for disposal of effluents in industries leads to severe pollution of natural resources in developing countries. These pollutants accompanied by solid waste are equally hazardous to biological growth. Natural attenuation of these pollutants was evidenced that involved degradation by native microbial communities. The current study encompasses the isolation of pesticide-degrading bacteria from the vicinity of pesticide manufacturing industries. Methods The isolation and identification of biodegrading microbes was done. An enrichment culture technique was used to isolate the selected pesticide-degrading bacteria from industrial waste. Results Around 20 different strains were isolated, among which six isolates showed significant pesticide biodegrading activity. After 16S rRNA analysis, two isolated bacteria were identified as Acinetobacter baumannii (5B) and Acidothiobacillus ferroxidans, and the remaining four were identified as different strains of Pseudomonas aeruginosa (1A, 2B, 3C, 4D). Phylogenetic analysis confirmed their evolution from a common ancestor. All strains showed distinctive degradation ability up to 36 hours. The Pseudomonas aeruginosa strains 1A and 4D showed highest degradation percentage of about 80% for DDT, and P. aeruginosa strain 3C showed highest degradation percentage, i.e., 78% for aldrin whilst in the case of malathion, A. baumannii and A. ferroxidans have shown considerable degradation percentages of 53% and 54%, respectively. Overall, the degradation trend showed that all the selected strains can utilize the given pesticides as sole carbon energy sources even at a concentration of 50 mg/mL. Conclusion This study provided strong evidence for utilizing these strains to remove persistent residual pesticide; thus, it gives potential for soil treatment and restoration.

A Flavobacterium sp. that degrades diazinon and parathion

1973 ◽  
Vol 19 (7) ◽  
pp. 873-875 ◽  
Author(s):  
N. Sethunathan ◽  
T. Yoshida
Keyword(s):  
Carbon Dioxide ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Enrichment Culture ◽  
Culture Technique ◽  
Mineral Medium ◽  
Paddy Water

A Flavobacterium sp., isolated from paddy water by enrichment culture technique, decomposed diazinon in a mineral medium as sole carbon source. The bacterium readily hydrolyzed diazinon to 2-isopropyl-6-methyl-4-hydroxy-pyrimidine which was then converted to carbon dioxide. The bacterium also converted parathion to p-nitrophenol. The enzyme involved in the hydrolysis was constitutive.

scholarly journals Pandrug-resistant Pseudomonas sp. expresses New Delhi metallo-β-lactamase-1 and consumes ampicillin as sole carbon source

2020 ◽  
Author(s):  
Vivek Kumar Ranjan ◽  
Shriparna Mukherjee ◽  
Subarna Thakur ◽  
Krutika Gupta ◽  
Ranadhir Chakraborty
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
New Delhi ◽  
Pseudomonas Sp

Effect of culture medium ions on chromate reduction by resting cells of Agrobacterium radiobacter

1993 ◽  
Vol 39 (3) ◽  
Author(s):  
Santiago LLovera ◽  
Ramon Bonet ◽  
MariaDolores Simon-Pujol ◽  
Francisco Congregado
Keyword(s):  
Culture Medium ◽  
Chromate Reduction ◽  
Resting Cells ◽  
Agrobacterium Radiobacter

scholarly journals Synergistic biodegradation of aromatic-aliphatic copolyester plastic by a marine microbial consortium

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Ingrid E. Meyer-Cifuentes ◽  
Johannes Werner ◽  
Nico Jehmlich ◽  
Sabine E. Will ◽  
Meina Neumann-Schaal ◽  
...  
Keyword(s):  
Sole Carbon Source ◽  
Microbial Consortium ◽  
Degradation Products ◽  
Enrichment Culture ◽  
Synthetic Polymers ◽  
Plastic Film ◽  
Marine Microorganisms ◽  
Community Members ◽  
Maximum Conversion ◽  
Degradation Step

AbstractThe degradation of synthetic polymers by marine microorganisms is not as well understood as the degradation of plastics in soil and compost. Here, we use metagenomics, metatranscriptomics and metaproteomics to study the biodegradation of an aromatic-aliphatic copolyester blend by a marine microbial enrichment culture. The culture can use the plastic film as the sole carbon source, reaching maximum conversion to CO2 and biomass in around 15 days. The consortium degrades the polymer synergistically, with different degradation steps being performed by different community members. We identify six putative PETase-like enzymes and four putative MHETase-like enzymes, with the potential to degrade aliphatic-aromatic polymers and their degradation products, respectively. Our results show that, although there are multiple genes and organisms with the potential to perform each degradation step, only a few are active during biodegradation.

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