UTILIZATION OF AROMATIC HYDROCARBONS BY ARTHROBACTER SPP.

1967 ◽  
Vol 13 (2) ◽  
pp. 205-211 ◽  
Author(s):  
I. L. Stevenson
Keyword(s):  
Carbon Source ◽  
Aromatic Hydrocarbons ◽  
Sole Carbon Source ◽  
Total Population ◽  
Residual Soil ◽  
Bacterial Isolates ◽  
Aromatic Substrates ◽  
Wide Range ◽  
Nutritional Studies ◽  
Different Soils

Bacterial isolates from a number of different soils were screened by growth observation and microscopic examination for Arthrobacter spp. Incidence of arthrobacter in the total population varied, but averaged around 15% in the soils investigated. One hundred and thirty arthrobacter isolates were tested for their ability to utilize aromatic hydrocarbons as their sole carbon source. Seventy-seven percent of these organisms were able to grow on at least two aromatic substrates and many were capable of growth on a wide range of these compounds. Nutritional studies indicated that arthrobacter with simple requirements were able to utilize the greatest number of aromatic hydrocarbons as their sole carbon source. The ability of the arthrobacter to metabolize aromatic compounds is discussed in terms of their possible role in the formation and turnover of residual soil organic matter.

scholarly journals Effect of Bacterial Isolates From Soil Samples on Bisphenol A

2020 ◽  
Vol 31 (1) ◽  
pp. 15
Author(s):  
Dr.Neihaya Heikmat Zaki
Keyword(s):  
Carbon Source ◽  
Bisphenol A ◽  
Sole Carbon Source ◽  
Soil Samples ◽  
Triazine Ring ◽  
Bacterial Isolates ◽  
Optimal Temperature ◽  
Tigris River ◽  
End Products ◽  
Proteus Penneri

Twenty five samples were collected from the soil around the Tigris River from different locations in Iraqi cities, and 45 bacterial isolates were obtained. Three of these isolates were further tested for their degrading capacity of Bisphenol A (BPA) in Basal Mineral Medium, included: Pseudomonas orizohibtanis, Escherishia coli and Proteus penneri. The optimal temperature for the removal of BPA was determined at 20˚C, 37˚ and 45˚C for 1, 5, and 15 days, and the degradation increased up to a temperature of 37°C. Growth test was performed on isolated bacteria with BisPhenol A as the sole carbon source, and with increasing incubation time, the culture grew almost linearly to 24 hours. BPA decreased after 1days after incubating with tested bacterial isolates, and almost broken after 5 days, while it disappeared after 15 days at 37C, and Pseudomonas orizohibtanis exhibited the best degradation of BPA. The absorbance peaks in the UV region appeared at 222 and 276 nm and attributed to the benzene ring and triazine ring respectively. The end products of BPA degradation were analyzed by GCMS after 15 days of incubation. The chromatogram for Pseudomanas orizohibtanis showed three peaks at retention times of 70, 210 and 280 min, and referred to hexasiloxane, heptasiloxane, and Octasiloxane respectively. The present study was aimed to isolate bacteria from the soil of the Tigris River, and determined the ability to degrade Bisphenol-A, and characterized the environmental conditions of bacterial growth, and then analysis the products of the degradation by GC-MS.

Acidobacteria from oligotrophic soil from the Cerrado can grow in a wide range of carbon source concentrations

2013 ◽  
Vol 59 (11) ◽  
pp. 746-753 ◽  
Author(s):  
Virgilio Hipólito Lemos de Castro ◽  
Luis Felipe Schroeder ◽  
Betania Ferraz Quirino ◽  
Ricardo Henrique Kruger ◽  
Cristine Chaves Barreto
Keyword(s):  
Carbon Source ◽  
16S Rrna ◽  
Growth Rates ◽  
Carboxymethyl Cellulose ◽  
Sole Carbon Source ◽  
Solid Medium ◽  
Colloidal Chitin ◽  
Brazilian Cerrado ◽  
Liquid Media ◽  
Wide Range

Soils from the Brazilian Cerrado are nutrient-poor, acidic, and aluminum-rich. A previous study revealed that members of the phylum Acidobacteria were predominant in these oligotrophic soils. Five acidobacteria from Cerrado soil were isolated on VL-55 medium containing 0.05% of xylan as carbon source. All isolates belong to the Acidobacteria subdivision 1, and their 16S rRNA showed similarities of 94.2%–96% with Acidobacterium capsulatum or 98.6% with Edaphobacter aggregans. All isolates were able to sustain growth in a wide range of carbon source concentrations. Growth occurred in all concentrations of arabinose, dextrose, and xylose; only one isolate did not grow on fructose. Isolates grew poorly on N-acetyl-d-glucosamine at all concentrations tested. In general, increasing concentrations of these monosaccharides did not inhibit growth rates. Isolates exhibited growth on solid medium containing xylan, carboxymethyl cellulose, and colloidal chitin; however, growth was observed on solid medium that did not contain these polysaccharides. These isolates may be able to use the solidifying agents tested (gellan gum or agar) as carbon source. This interpretation is supported by the absence of growth in liquid media containing chitin or carboxymethyl cellulose at 0.05% as sole carbon source, whereas growth in the same conditions using xylan was confirmed.

scholarly journals Isolation and Potential Testing of North Sumatera Berastagi Agricultural Soil In Degrading Marshal Insecticide With Carbosulfan Active Material

2020 ◽  
Vol 2 (02) ◽  
pp. 147-156
Author(s):  
Nunuk Priyani
Keyword(s):  
Carbon Source ◽  
Agricultural Soil ◽  
Sole Carbon Source ◽  
Active Material ◽  
Bacterial Isolates ◽  
Selective Media

The isolation of bacteria from Berastagi agricultural soil North Sumatera has been done. The aim is to evaluate their ability in degrading carbosulfan. Sixteen bacterial isolates were obtained using selective media Bushnel Hass Agar (BHA) containing 12 ppm of carbosulfan. The parameters observed were the growth of isolates, biosurfactant activity, biosurfactant concentration, and the residue of carbosulfan after 21 days of incubation. The result showed that all isolates were able to degrade carbosulfan as the sole carbon source. Two isolates namely JBM 3 (isolate from citrus agricultural soil Berastagi) and KBM 1 (isolate from cabbage agricultural soil Berastagi) were selected for further test to determine their ability to degrade carbosulfan. The results showed that both of the isolates were able to degrade carbosulfan. Compare to control, isolate JBM 3 was able to decrease the concentration of carbosulfan by 33.33%, while isolate KBM 1 was able to reduce carbosulfan concentration up to 40.47%.

scholarly journals Glyphosate Utilization as the Source of Carbon: Isolation and Identification of new Bacteria

2011 ◽  
Vol 8 (4) ◽  
pp. 1582-1587 ◽  
Author(s):  
M. Mohsen Nourouzi ◽  
T. G. Chuah ◽  
Thomas S. Y. Choong ◽  
C. J. Lim
Keyword(s):  
Carbon Source ◽  
Stenotrophomonas Maltophilia ◽  
Sole Carbon Source ◽  
Enrichment Culture ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Providencia Alcalifaciens ◽  
Mixed Bacteria ◽  
Aminomethyl Phosphonic Acid

Mixed bacteria from oil palm plantation soil (OPS) were isolated to investigate their ability to utilize glyphosate as carbon source. Results showed that approximately all of the glyphosate was converted to aminomethyl-phosphonic acid (AMPA) (99.5%). It is worthy to note that mixed bacteria were able to degrade only 2% of AMPA to further metabolites. Two bacterial strainsi.e. Stenotrophomonas maltophiliaandProvidencia alcalifacienswere obtained from enrichment culture. Bacterial isolates were cultured individually on glyphosate as a sole carbon source. It was observed that both isolates were able to convert glyphosate to AMPA.

Selective degradation of biphenyl and methylbiphenyls in crude oil by two strains of marine bacteria

1983 ◽  
Vol 29 (5) ◽  
pp. 497-503 ◽  
Author(s):  
P. M. Fedorak ◽  
D. W. S. Westlake
Keyword(s):  
Carbon Source ◽  
Benzoic Acid ◽  
Crude Oil ◽  
Marine Sediment ◽  
Marine Bacteria ◽  
Sole Carbon Source ◽  
Bacterial Isolates ◽  
Aromatic Fraction ◽  
Selective Degradation ◽  
Prudhoe Bay

Bacterial isolates were obtained from marine sediment and water enrichment cultures which had been maintained for 3 years by monthly transfers on artificial seawater with Prudhoe Bay crude oil as the sole carbon source. Capillary gas chromatographic analyses showed that two isolates selectively degraded only biphenyl, 3-methylbiphenyl, 4-methylbiphenyl, and three other minor, unidentified compounds in the aromatic fraction. No degradation was detected in the saturate fraction, nor in the sulfur heterocyclic component of the oil. When grown on any of the pure biphenyls, these isolates produced a transient, yellow intermediate which had the spectrophotometric characteristics of α-hydroxymuconic semialdehydes. Growth on either methylbiphenyl compound produced a methylbenzoic acid, indicating that the nonmethylated ring was the first to be cleaved. The isolates, identified as Alcaligenes sp. and Acinetobacter sp., were also able to grow on benzoic acid, 3-, and 4-methylbenzoic acids, indicating that they could further metabolize the aromatic acid intermediates.

scholarly journals Polycyclic Aromatic Hydrocarbons Degradation by Aquatic Bacteria Isolated from Khazar Sea, the World’s Largest Lake

2020 ◽  
Vol 27 (1) ◽  
pp. 121-130
Author(s):  
Vida Ebrahimi ◽  
Shirin Eyvazi ◽  
Soheila Montazersaheb ◽  
Parivar Yazdani ◽  
Mohammad Amin Hejazi ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Carbon Source ◽  
Aromatic Hydrocarbons ◽  
Sea Water ◽  
Environmental Pollutants ◽  
Bacterial Isolates ◽  
Mazandaran Province ◽  
Degrading Bacteria ◽  
Lung Dysfunction ◽  
Polycyclic Aromatic

Background: Aquatic microorganisms have an important role in the bioremediation of environmental pollutants. Polycyclic Aromatic Hydrocarbons (PAHs) are described as dangerous pollutants that can bind covalently to the nucleic acids, causing mutations. Therefore, they have carcinogenic and toxic properties. Also, are involved in diseases such as asthma, lung dysfunction, and chronic bronchitis. This study aimed to isolate and characterize aquatic bio-degrading bacteria from the world’s largest lake, Khazar, with the ability to use PAHs as only carbon source. Methods: Samples were taken from the estuary of Siah Rud River (Mazandaran province, Iran) and Fereydunkenar beach leading to isolation of twenty-three bacteria on marine agar and sea water media. The isolates were cultured on separate ONR7a medium, each supplemented with only one PAH; as the sole carbon source; including naphthalene, phenanthrene, and anthracene. Results: Eleven bacterial isolates were able to grow on supplemented media: TBZ-E1, TBZ-E2, TBZ-E3, TBZ-S12, TBZ-S16, TBZ-E20, TBZ-SF2, TBZ-F1, TBZ-F2, TBZ-F3 and TBZ2. These isolates belong to Alteromonas, Marivivens, Pseudoalteromonas, Vibrio, Shewanella, Photobacterium, Mycobacterium and Pseudomonas genera. The qualitative analysis showed that the consortium of isolates TBZ-F1, TBZ-F2, TBZ-F3, TBZ-SF2, and TBZ2 displayed the highest degradation rate for phenanthrene and naphthalene. Naphthalene, phenanthrene, and anthracene were potently degraded by TBZ2 and TBZ-SF2 and accordingly were subjected to measure degradation potential of mentioned PAHs. Conclusion: The bacterial isolates of Caspian lake have a critical duty in biodegradation of PAHs. These isolates are representative samples of the bacterial population of this lake, participating in the purification process of this habitat.

scholarly journals Pandrug-resistant Pseudomonas sp. expresses New Delhi metallo-β-lactamase-1 and consumes ampicillin as sole carbon source

2020 ◽  
Author(s):  
Vivek Kumar Ranjan ◽  
Shriparna Mukherjee ◽  
Subarna Thakur ◽  
Krutika Gupta ◽  
Ranadhir Chakraborty
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
New Delhi ◽  
Pseudomonas Sp

scholarly journals Study of a plugging microbial consortium using crude oil as sole carbon source

2008 ◽  
Vol 5 (4) ◽  
pp. 367-374 ◽  
Author(s):  
Jing Wang ◽  
Guiwen Yan ◽  
Mingquan An ◽  
Jieli Liu ◽  
Houming Zhang ◽  
...  
Keyword(s):  
Carbon Source ◽  
Crude Oil ◽  
Sole Carbon Source ◽  
Microbial Consortium
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