A glycerol-inducible thermostable lipase from Bacillus sp.: medium optimization by a Plackett–Burman design and by response surface methodology

2004 ◽  
Vol 50 (5) ◽  
pp. 361-368 ◽  
Author(s):  
Namita Gupta ◽  
Gauri Mehra ◽  
Rani Gupta
Keyword(s):  
Enzyme Production ◽  
Medium Optimization ◽  
Lipase Production ◽  
Inoculum Density ◽  
Bacillus Sp ◽  
Optimal Composition ◽  
Thermostable Lipase ◽  
Burman Design ◽  
The One ◽  
Plackett Burman Design

The production of a neutral lipase from a Bacillus sp. was improved tremendously (193-fold) following media optimization involving both the "one-at-a-time" and the statistical designing approaches. The present lipase was poorly induced by oils, instead its production was induced in the presence of sugars and sugar alcohols, mainly galactose, lactose, glycerol, and mannitol. A high inoculum density of 15% v/v (A550 = 0.8) led to maximum lipase production. Interestingly, the enzyme induction was growth independent, a property very different from most of the lipases investigated to date. The optimal composition of the growth medium to achieve maximum lipase production was determined to be as follows: NH4Cl, 35 g·L–1; glycerol, 10 mL·L–1; K2HPO4, 3 g·L–1; KH2PO4, 1 g·L–1; MgSO4·7H2O, 0.1 g·L–1; glucose, 2 g·L–1; MgCl2, 0.6 mmol·L–1, with 15% inoculum density and an incubation period of 24 h. About 62 U·mL–1 of enzyme production was achieved in the optimized medium.Key words: lipase, glycerol inducible, statistical designing.

scholarly journals Production of xylanase by Fusarium oxysporum using agro-industrial waste

2021 ◽  
Vol 17 (8) ◽  
Author(s):  
Lunara Thais Alves de Bastos ◽  
Ryhára Dias Batista ◽  
Alanna Cristinne Martins Lima ◽  
Iara Leandro dos Santos ◽  
Fabrício Coutinho de Paula-Elias ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Low Cost ◽  
Agricultural Waste ◽  
Corn Straw ◽  
Xylanase Production ◽  
Burman Design ◽  
The One ◽  
Plackett Burman Design ◽  
Submerged Conditions ◽  
Selection Of

This study aimed to optimize the production of xylanase, under submerged conditions using Fusarium oxysporum. Firstly, it was carried out the selection of the best source of carbon from agricultural waste, such as soybean husk, cassava peel, pineapple crown, corn straw, rice husk, bacaba, barley bagasse and, corncob. They were milled and sieved with a maximum granulation of 1 mm. The cultures were carried out by 132 hours 30 °C and 180 rpm. Among the agroindustrial residues, corn straw was the one that stood out with a greater enzymatic activity of 12.6 U/mL. In the second step, it was used Plackett-Burman design to screen the nutrients sources important to xylanase production. Thus, independent variables significant were urea and MgSO4. These variables selected by Plackett-Burman were then used in a Central Composite Rotational Design present activity of 26.6 U/mL and the predicted was 34.5 U/mL. So, the xylanase production by F. oxysporum can be optimize using corn straw, a low-cost waste found in large quantities.

scholarly journals Statistical Optimization by Response Surface Methodology to Enhance Lipase Production by Aspergillus fumigatus

2019 ◽  
Vol 13 (1) ◽  
pp. 86-93 ◽  
Author(s):  
Akshita Mehta ◽  
Roji Sharma ◽  
Reena Gupta
Keyword(s):  
Aspergillus Fumigatus ◽  
Response Surface ◽  
Incubation Time ◽  
Lipase Production ◽  
Inoculum Size ◽  
Coefficient Of Determination ◽  
Burman Design ◽  
Commercial Applications ◽  
Plackett Burman Design ◽  
Central Composite

Background:Lipases have various commercial applications and microorganisms serve as a potential source of production.Objective:The aim of this paper was to study the effect of interactions among different production parameters on lipase yield ofAspergillus fumigatus.Method:Plackett Burman and Central Composite Design (CCD) were established by using Design Expert software 10.0.Results:In the present study, interactions were studied for six different variables such as inoculum size, pH, temperature, galactose concentration, peptone concentration and incubation time. In Plackett-Burman design, galactose concentration, peptone concentration, pH and incubation time were found to be important factors. Using the statistical approach, the optimum factors were found to be as: galactose concentration (1.5%), peptone concentration (1.8%), pH (10.0) and incubation time (72 h) at 45°C under response surface curves. Upon statistical analysis, the coefficient of determination (R2) obtained was 0.9318 which showed that the model was significant.Conclusion:The statistical tools used predicted the optimal conditions for the production of the lipase. The optimized parameters were galactose concentration 1.5%, peptone concentration 1.4%, temperature 45°C, pH 10.0 and incubation time of 72 h for obtaining a maximum lipase activity of 6.22 U/ml.

scholarly journals Potential of Aspergilus Niger MT809753 for Bio-treatment Paper Industry Wastewater After Screening Effective Factors on Production of Cellulase Enzyme by Plackett-burman Design

2021 ◽  
Author(s):  
Marwa El-Sesy ◽  
Amira M Aly
Keyword(s):  
Rice Straw ◽  
Enzyme Production ◽  
Paper Industry ◽  
Cellulase Production ◽  
Barley Straw ◽  
Cellulase Enzyme ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Aspergilus Niger ◽  
Industry Wastewater

Abstract A microorganism capable of degrading cellulose present in rice straw was isolated from wastewater samples and identified as Aspergilus niger MT809753 by 18S rDNA. In the present study various cheap agronomic cellulosic wastes as (cotton seed husks, barley straw, rice straw and maize straw) were utilized as crude inducers for the cellulase enzyme production and represent the carbon source for isolates where cellulose activity was measured by (DNS) method. The highest cellulases enzyme production was obtained by fungal isolate Aspergilus niger MT809753 within 24 hours (0.532 IU/ml) using rice straw. Plackett-Burman design was used as conventional method for statistically screening of different variables. Nine variables of the production process were selected. The results illustrate those seven variables, namely as (inoculum size, substrate concentration, incubation temperature, pH, shaking conditions, and incubation time and peptone concentration) had influence with high confidence levels, while the remaining two variables did not show a significant effect on cellulase production. After using response optimization the experiment was performed and the obtained cellulase production was 1.08 IU/ml. A bench scale study was performed to examine paper industry wastewater treatment efficiency by Aspergillus Niger MT809753. Results reveal that organisms have proved their bioremediation potency in treatment of paper industry effluent. The importance of the research stems from the fact that it sheds light on the role of some fungi in the production of the cellulase enzyme. So our goal is to obtain local isolates from fungi having a high ability to produce the cellulase enzyme, as well as developing an effective treatment processes to get rid of environmental cellulosic pollution and utilization of cellulosic wastes as cheap carbon sources.

scholarly journals Application of Plackett-Burman Experimental Design for Lipase Production by Aspergillus niger Using Shea Butter Cake

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Aliyu Salihu ◽  
Muntari Bala ◽  
Shuaibu M. Bala
Keyword(s):  
Aspergillus Niger ◽  
Statistical Approach ◽  
Tween 80 ◽  
Lipase Production ◽  
Rapid Identification ◽  
Shea Butter ◽  
Medium Components ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Main Substrate

Plackett-Burman design was used to efficiently select important medium components affecting the lipase production by Aspergillus niger using shea butter cake as the main substrate. Out of the eleven medium components screened, six comprising of sucrose, (NH4)2SO4, Na2HPO4, MgSO4, Tween-80, and olive oil were found to contribute positively to the overall lipase production with a maximum production of 3.35 U/g. Influence of tween-80 on lipase production was investigated, and 1.0% (v/w) of tween-80 resulted in maximum lipase production of 6.10 U/g. Thus, the statistical approach employed in this study allows for rapid identification of important medium parameters affecting the lipase production, and further statistical optimization of medium and process parameters can be explored using response surface methodology.

scholarly journals Screening and Selection of Media Components for Protease Production by Bacillus sp. EBTA6 Using Plackett–Burman Design

2020 ◽  
Vol 8 (6) ◽  
pp. 1250-1255
Author(s):  
Fikriye Alev Akçay ◽  
Ayşe Avcı
Keyword(s):  
Yeast Extract ◽  
Protease Activity ◽  
Inoculum Size ◽  
Protease Production ◽  
Bacillus Sp ◽  
Medium Components ◽  
Dipotassium Phosphate ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Selection Of

In this study, effects of medium components and inoculum size on the protease production by Bacillus sp. EBTA6 that was isolated from a home-made Tarhana sample were investigated. The cell-free supernatant of bacterium cultured on a shaking incubator for 24 h was used to test protease activity as the response. With a total number of 11 factors, 12 different experiments were run and the highest experimental protease activity was measured as 2280.4 U/mL. Results were analyzed statistically by ANOVA and the most efficient factors were detected as yeast extract, dipotassium phosphate, casein, and peptone with a contribution of 93.78, 2.19, 1.96, 1.31%, respectively. For validation of the selected factors, a further experiment was performed by using of yeast extract (9.98 g/L), dipotassium phosphate (1.27 g/L), casein (8.69 g/L), and peptone (9.88 g/L) obtained from the design equation. The experimental response was found as 2411.4 U/mL which was only 5.5% higher than the predicted response showing that the model was applicable.

scholarly journals Medium optimization and subsequent fermentative regulation for the scaled-up production of anti-tuberculosis drug leads ilamycin-E1/E2

2021 ◽  
Author(s):  
Zhiying Fan ◽  
Nian Tong ◽  
Zhoukang Zhuang ◽  
Cheng Ma ◽  
Junying Ma ◽  
...  
Keyword(s):  
Medium Optimization ◽  
Feeding Strategy ◽  
Malt Extract ◽  
Ph Change ◽  
Lead Compounds ◽  
Negative Factor ◽  
Burman Design ◽  
Drug Leads ◽  
Plackett Burman Design ◽  
Continuous Pulse

Tuberculosis (TB) and its emerged drug resistance exert huge threats on the global health, therefore development of novel anti-TB antibiotics is very essential. Ilamycin-E1/E2 is a pair of cycloheptapeptide enantiomers obtained from a marine-derived Streptomyces atratus SCSIO ZH16-ΔilaR mutant, and become promising anti-TB lead compounds due to their significant anti-TB activities, but their low titer hampered the further clinical development. In this work, the statistical Plackett-Burman design (PBD) model was applied to screen out bacterial peptone as the only significant but negative factor affecting the ilamycin-E1/E2 production. Subsequent single factor optimization revealed that replacement of bacterial peptone with malt extract eliminated the accumulation of porphyrin-type competitive byproduct, and the titer of ilamycin-E1/E2 in shaking flasks was improved from original 13.6±0.8 to 142.7±5.7 mg/L for about 10.5 folds. Furthermore, a pH coordinated feeding strategy was first adopted in scaled-up production of ilamycin-E1/E2. The obtained titer of ilamycin-E1/E2 in 30L was 169.8±2.5 mg/L, while in 300L fermentor was only 131.5±7.5 mg/L due to the unsynchronization of feeding response and pH change. Therefore, the continuous pulse feeding strategy was further applied in 300L fermentor and finally achieved 415.7±29.2 mg/L ilamycin-E1/E2, which represented about 30.5 folds improvement at last. Our work provided the solid basis to achieve sufficient ilamycin-E1/E2 lead compounds and support their potential anti-TB drug development.

scholarly journals Medium Optimization by Response Surface Methodology for Improved Cholesterol Oxidase Production by a Newly Isolated Streptomyces rochei NAM-19 Strain

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Elsayed Ahmed Elsayed ◽  
Nayera Ahmed Abdelwahed
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Enzyme Production ◽  
Medium Optimization ◽  
Design Method ◽  
Cholesterol Oxidase ◽  
Malt Extract ◽  
Optimization Strategy ◽  
Egyptian Soil ◽  
Burman Design

Cholesterol oxidase is an alcohol oxidoreductase flavoprotein with wide biotechnological applications. The current work describes the isolation of a potential cholesterol oxidase producing streptomycete from Egyptian soil. The isolated strain produced cholesterol oxidase in submerged culture using a medium containing glucose, yeast extract, malt extract, and CaCO3 with the addition of cholesterol as an inducer. The isolated strain was identified as Streptomyces rochei NAM-19 based on 16S rRNA sequencing and phylogeny. Optimization of cholesterol oxidase production has been carried out using response surface methodology. The Plackett-Burman design method was used to evaluate the significant components of the production medium followed by Box-Behnken experimental design to locate the true optimal concentrations, which are significantly affecting enzyme production. Results showed that the predicted enzyme response could be closely correlated with the experimentally obtained production. Furthermore, the applied optimization strategy increased volumetric enzyme production by 2.55 times (65.1 U/mL) the initial production obtained before medium optimization (25.5 U/mL).

scholarly journals Statistical Based Bioprocess Design for Improved Production of Amylase from Halophilic Bacillus sp. H7 Isolated from Marine Water

Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 2833
Author(s):  
J. N. Bandal ◽  
V. A. Tile ◽  
R. Z. Sayyed ◽  
H. P. Jadhav ◽  
N. I. Wan Azelee ◽  
...  
Keyword(s):  
Catalytic Efficiency ◽  
Fold Increase ◽  
Soluble Starch ◽  
Statistical Optimization ◽  
Bacillus Sp ◽  
Bioprocess Design ◽  
Medium Components ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Central Composite

Amylase (EC 3.2.1.1) enzyme has gained tremendous demand in various industries, including wastewater treatment, bioremediation and nano-biotechnology. This compels the availability of enzyme in greater yields that can be achieved by employing potential amylase-producing cultures and statistical optimization. The use of Plackett–Burman design (PBD) that evaluates various medium components and having two-level factorial designs help to determine the factor and its level to increase the yield of product. In the present work, we are reporting the screening of amylase-producing marine bacterial strain identified as Bacillus sp. H7 by 16S rRNA. The use of two-stage statistical optimization, i.e., PBD and response surface methodology (RSM), using central composite design (CCD) further improved the production of amylase. A 1.31-fold increase in amylase production was evident using a 5.0 L laboratory-scale bioreactor. Statistical optimization gives the exact idea of variables that influence the production of enzymes, and hence, the statistical approach offers the best way to optimize the bioprocess. The high catalytic efficiency (kcat/Km) of amylase from Bacillus sp. H7 on soluble starch was estimated to be 13.73 mL/s/mg.

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