scholarly journals Plackett-Burman design for screening of process components and their effects on production of lactase by newly isolated Bacillus sp. VUVD101 strain from Dairy effluent

2018 ◽  
Vol 7 (4) ◽  
pp. 543-546 ◽  
Author(s):  
Abraham Peele Karlapudi ◽  
S. Krupanidhi ◽  
Rajeswara Reddy E. ◽  
Indira M. ◽  
N. Bobby Md. ◽  
...  
Keyword(s):  
Bacillus Sp ◽  
Dairy Effluent ◽  
Burman Design ◽  
Plackett Burman Design

scholarly journals Screening and Selection of Media Components for Protease Production by Bacillus sp. EBTA6 Using Plackett–Burman Design

2020 ◽  
Vol 8 (6) ◽  
pp. 1250-1255
Author(s):  
Fikriye Alev Akçay ◽  
Ayşe Avcı
Keyword(s):  
Yeast Extract ◽  
Protease Activity ◽  
Inoculum Size ◽  
Protease Production ◽  
Bacillus Sp ◽  
Medium Components ◽  
Dipotassium Phosphate ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Selection Of

In this study, effects of medium components and inoculum size on the protease production by Bacillus sp. EBTA6 that was isolated from a home-made Tarhana sample were investigated. The cell-free supernatant of bacterium cultured on a shaking incubator for 24 h was used to test protease activity as the response. With a total number of 11 factors, 12 different experiments were run and the highest experimental protease activity was measured as 2280.4 U/mL. Results were analyzed statistically by ANOVA and the most efficient factors were detected as yeast extract, dipotassium phosphate, casein, and peptone with a contribution of 93.78, 2.19, 1.96, 1.31%, respectively. For validation of the selected factors, a further experiment was performed by using of yeast extract (9.98 g/L), dipotassium phosphate (1.27 g/L), casein (8.69 g/L), and peptone (9.88 g/L) obtained from the design equation. The experimental response was found as 2411.4 U/mL which was only 5.5% higher than the predicted response showing that the model was applicable.

scholarly journals Statistical Based Bioprocess Design for Improved Production of Amylase from Halophilic Bacillus sp. H7 Isolated from Marine Water

Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 2833
Author(s):  
J. N. Bandal ◽  
V. A. Tile ◽  
R. Z. Sayyed ◽  
H. P. Jadhav ◽  
N. I. Wan Azelee ◽  
...  
Keyword(s):  
Catalytic Efficiency ◽  
Fold Increase ◽  
Soluble Starch ◽  
Statistical Optimization ◽  
Bacillus Sp ◽  
Bioprocess Design ◽  
Medium Components ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Central Composite

Amylase (EC 3.2.1.1) enzyme has gained tremendous demand in various industries, including wastewater treatment, bioremediation and nano-biotechnology. This compels the availability of enzyme in greater yields that can be achieved by employing potential amylase-producing cultures and statistical optimization. The use of Plackett–Burman design (PBD) that evaluates various medium components and having two-level factorial designs help to determine the factor and its level to increase the yield of product. In the present work, we are reporting the screening of amylase-producing marine bacterial strain identified as Bacillus sp. H7 by 16S rRNA. The use of two-stage statistical optimization, i.e., PBD and response surface methodology (RSM), using central composite design (CCD) further improved the production of amylase. A 1.31-fold increase in amylase production was evident using a 5.0 L laboratory-scale bioreactor. Statistical optimization gives the exact idea of variables that influence the production of enzymes, and hence, the statistical approach offers the best way to optimize the bioprocess. The high catalytic efficiency (kcat/Km) of amylase from Bacillus sp. H7 on soluble starch was estimated to be 13.73 mL/s/mg.

A glycerol-inducible thermostable lipase from Bacillus sp.: medium optimization by a Plackett–Burman design and by response surface methodology

2004 ◽  
Vol 50 (5) ◽  
pp. 361-368 ◽  
Author(s):  
Namita Gupta ◽  
Gauri Mehra ◽  
Rani Gupta
Keyword(s):  
Enzyme Production ◽  
Medium Optimization ◽  
Lipase Production ◽  
Inoculum Density ◽  
Bacillus Sp ◽  
Optimal Composition ◽  
Thermostable Lipase ◽  
Burman Design ◽  
The One ◽  
Plackett Burman Design

The production of a neutral lipase from a Bacillus sp. was improved tremendously (193-fold) following media optimization involving both the "one-at-a-time" and the statistical designing approaches. The present lipase was poorly induced by oils, instead its production was induced in the presence of sugars and sugar alcohols, mainly galactose, lactose, glycerol, and mannitol. A high inoculum density of 15% v/v (A550 = 0.8) led to maximum lipase production. Interestingly, the enzyme induction was growth independent, a property very different from most of the lipases investigated to date. The optimal composition of the growth medium to achieve maximum lipase production was determined to be as follows: NH4Cl, 35 g·L–1; glycerol, 10 mL·L–1; K2HPO4, 3 g·L–1; KH2PO4, 1 g·L–1; MgSO4·7H2O, 0.1 g·L–1; glucose, 2 g·L–1; MgCl2, 0.6 mmol·L–1, with 15% inoculum density and an incubation period of 24 h. About 62 U·mL–1 of enzyme production was achieved in the optimized medium.Key words: lipase, glycerol inducible, statistical designing.

Assessment of liquid chromatographic method robustness by use of Plackett-Burman design

2010 ◽  
Vol 22 (2) ◽  
pp. 281-296 ◽  
Author(s):  
M. Mašković ◽  
B. Jančić-Stojanović ◽  
A. Malenović ◽  
D. Ivanović ◽  
M. Medenica
Keyword(s):  
Chromatographic Method ◽  
Liquid Chromatographic Method ◽  
Burman Design ◽  
Liquid Chromatographic ◽  
Plackett Burman Design

Using a Plackett–Burman design to maximise yield of rosemary essential oil by distillation

2021 ◽  
Vol 166 ◽  
pp. 113488
Author(s):  
Agnese Spadi ◽  
Giulia Angeloni ◽  
Lorenzo Guerrini ◽  
Ferdinando Corti ◽  
Marco Michelozzi ◽  
...  
Keyword(s):  
Essential Oil ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Rosemary Essential Oil

scholarly journals Analysis of insecticide residues in honey by liquid chromatography tandem mass spectrometry using QuEChERS optimized by the Plackett Burman design

2021 ◽  
Vol 19 (1) ◽  
pp. 326-332
Author(s):  
Ana Paula Ferreirade Souza ◽  
Mateus Henrique Petrarca ◽  
Patrícia Aparecida de Campos Braga ◽  
Nadia Regina Rodrigues ◽  
Felix Guillermo Reyes Reyes
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Tandem Mass ◽  
Insecticide Residues ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Burman Design ◽  
Plackett Burman Design

scholarly journals Use of chemometric techniques to design a microbiological method for sulfonamide detection in milk

2013 ◽  
Vol 31 (No. 6) ◽  
pp. 627-632 ◽  
Author(s):  
O.G. Nagel ◽  
M.P. Molina ◽  
R.L. Althaus
Keyword(s):  
Experimental Design ◽  
Logistic Model ◽  
Logistic Regression Model ◽  
Culture Medium ◽  
Spore Concentration ◽  
Microbiological Method ◽  
Burman Design ◽  
Detection Capabilities ◽  
Sulfonamide Residues ◽  
Plackett Burman Design

We proposed an experimental design of a microbial bioassay of dichotomous response (positive or negative) using Bacillus subtilis BGA for the detection of sulfonamide residues. In the first stage, the bioassay response time was reduced to 6 h by increasing the spore concentration of B. subtilis. Then, the effects of spore, indicator, trimethoprim (TMP) concentration, and volume of the culture medium were examined with a Plackett Burman design (2<sup>4-1</sup>). Finally, the effect of TMP concentration on the method detection capabilities and specificity was analysed using a logistic model with interaction. The detection capabilities of sulfonamides in milk are close to the MRLs when using 500 mg/l of TMP in the culture medium of the bioassay. It is concluded that the experimental design techniques and a logistic regression model can be used to design successfully a dichotomous response bioassay.

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