scholarly journals Statistical Based Bioprocess Design for Improved Production of Amylase from Halophilic Bacillus sp. H7 Isolated from Marine Water

Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 2833
Author(s):  
J. N. Bandal ◽  
V. A. Tile ◽  
R. Z. Sayyed ◽  
H. P. Jadhav ◽  
N. I. Wan Azelee ◽  
...  
Keyword(s):  
Catalytic Efficiency ◽  
Fold Increase ◽  
Soluble Starch ◽  
Statistical Optimization ◽  
Bacillus Sp ◽  
Bioprocess Design ◽  
Medium Components ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Central Composite

Amylase (EC 3.2.1.1) enzyme has gained tremendous demand in various industries, including wastewater treatment, bioremediation and nano-biotechnology. This compels the availability of enzyme in greater yields that can be achieved by employing potential amylase-producing cultures and statistical optimization. The use of Plackett–Burman design (PBD) that evaluates various medium components and having two-level factorial designs help to determine the factor and its level to increase the yield of product. In the present work, we are reporting the screening of amylase-producing marine bacterial strain identified as Bacillus sp. H7 by 16S rRNA. The use of two-stage statistical optimization, i.e., PBD and response surface methodology (RSM), using central composite design (CCD) further improved the production of amylase. A 1.31-fold increase in amylase production was evident using a 5.0 L laboratory-scale bioreactor. Statistical optimization gives the exact idea of variables that influence the production of enzymes, and hence, the statistical approach offers the best way to optimize the bioprocess. The high catalytic efficiency (kcat/Km) of amylase from Bacillus sp. H7 on soluble starch was estimated to be 13.73 mL/s/mg.

scholarly journals Medium Optimization for Spore Production of a Straw-Cellulose Degrading Actinomyces Strain under Solid-State Fermentation Using Response Surface Method

2020 ◽  
Vol 12 (21) ◽  
pp. 8893
Author(s):  
Huanran Liu ◽  
Dan Zhang ◽  
Xia Zhang ◽  
Chuanzhi Zhou ◽  
Pei Zhou ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Inorganic Nitrogen ◽  
Nitrogen Sources ◽  
Spore Production ◽  
Steepest Ascent ◽  
Medium Components ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Central Composite

The strains capable of degrading cellulose have attracted much interest because of their applications in straw resource utilization in solid-state fermentation (SSF). However, achieving high spore production in SSF is rarely reported. The production of spores from Streptomyces griseorubens JSD-1 was investigated in shaker-flask cultivation in this study. The optimal carbon, organic nitrogen and inorganic nitrogen sources were sucrose, yeast extract and urea, respectively. Plackett–Burman design (PBD) was adopted to determine the key medium components, and the concentration levels of three components (urea, NaCl, MgSO4·7H2O) were optimized with the steepest ascent path and central composite design (CCD), achieving 1.72 × 109 CFU/g of spore production. Under the optimal conditions (urea 2.718% w/v, NaCl 0.0697% w/v, MgSO4·7H2O 0.06956% w/v), the practical value of spore production was 1.69 × 109 CFU/g. The determination coefficient (R2) was 0.9498, which ensures an adequate credibility of the model.

Medium Optimization for the Production of Phytase by Recombinant Pichia pastoris Grown on Glycerol

2011 ◽  
Vol 9 (1) ◽  
Author(s):  
Min Liu ◽  
Gabriel Potvin ◽  
Yiru Gan ◽  
Zhanbin Huang ◽  
Zisheng Zhang
Keyword(s):  
Pichia Pastoris ◽  
Phytase Production ◽  
Medium Components ◽  
Gap Promoter ◽  
Recombinant Pichia Pastoris ◽  
Burman Design ◽  
Statistical Designs ◽  
Plackett Burman Design ◽  
Central Composite ◽  
Three Components

Based on statistical designs, minimal salts medium, commonly used for yeast cultivation, was optimized to maximize GAP promoter-mediated phytase production by recombinant Pichia pastoris grown on glycerol. A Plackett-Burman design was followed to screen medium components to determine those that significantly affected phytase production. Of the 8 components studied, the concentrations of K2SO4, CaSO4•2H2O and MgSO4•7H2O were identified as having a significant effect. These three components were subsequently optimized by response surface methodology using a central composite design. The optimal concentrations of the three components, leading to a maximal extracellular phytase activity of 161.64 U/ml, were K2SO4 13.25g/l, CaSO4•2H2O 1.03g/l and MgSO4•7H2O 17.94g/l. The activity measured in cultures using optimized growth medium is significantly higher than the 73.31 U/ml measured in cultures using standard minimal salts media. The theoretical phytase yields predicted by the developed model were very close to experimentally obtained values.

Valorization of de-oiled cakes for xylanase production and optimization using central composite design by Trichoderma koeningi isolate

2017 ◽  
Vol 42 (3) ◽  
Author(s):  
Ramesh Bandikari ◽  
Umamahesh Katike ◽  
Naga Sivudu Seelam ◽  
Vijaya Sarathi Reddy Obulam
Keyword(s):  
Central Composite Design ◽  
18S Rrna ◽  
Fermentation Medium ◽  
Xylanase Production ◽  
Corn Cobs ◽  
Medium Components ◽  
The Media ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Central Composite

AbstractAimEvaluation of the medium components and conditions for the optimization of xylanase production in submerged fermentation byMethodswas identified by the 18s rRNA. NaOH pre-treated corn cobs were used in order to reduce the crystallinity of corn cobs. De-oiled cakes proximate composition was analyzed according to AOAC. Plackett-Burman design (PBD) was used to screen the 19 media variables that affect xylanase production and optimized the media by central composite design (CCD).ResultsThe isolate was identified asConclusionNaOH pre-treated corn cobs were used with mustard cake powder as fermentation medium constituent to induce the xylanase production. From the results we conclude that mustard cake induced the xylanase production.

scholarly journals Statistical optimization of medium components and physicochemical parameters to simultaneously enhance bacterial growth and esterase production by Bacillus thuringiensis

2016 ◽  
Vol 62 (1) ◽  
pp. 24-34 ◽  
Author(s):  
Cintia Anabela Mazzucotelli ◽  
María del Rosario Moreira ◽  
María Roberta Ansorena
Keyword(s):  
Bacillus Thuringiensis ◽  
Bacterial Growth ◽  
Physicochemical Parameters ◽  
Desirability Function ◽  
Fold Increase ◽  
Simultaneous Optimization ◽  
Medium Components ◽  
Burman Design ◽  
Esterase Production ◽  
Plackett Burman Design

Bacillus thuringiensis is a genus extensively studied because of its high potential for biotechnological application, principally in biocontrol techniques. However, the optimization of esterase production by this strain has been scarcely studied. The aim of this work was to select and optimize the physicochemical and nutritional parameters that significantly influence the growth and esterase production of B. thuringiensis. To this purpose, 6 nutritional factors and 2 physicochemical parameters were evaluated using a Plackett–Burman design. Significant variables were optimized using a Box–Behnken design and through the desirability function to select the levels of the variables that simultaneously maximize microbial growth and esterase production. The optimum conditions resulting from simultaneous optimization of the responses under study were found to be 1 g/L glucose, 15 g/L peptone, and 3.25 g/L NaCl. Under these optimal conditions, it was possible to achieve a 2.5 log CFU/mL increase in bacterial growth and a 113-fold increase in esterase productivity, compared with minimal medium without agitation.

scholarly journals Screening and Selection of Media Components for Protease Production by Bacillus sp. EBTA6 Using Plackett–Burman Design

2020 ◽  
Vol 8 (6) ◽  
pp. 1250-1255
Author(s):  
Fikriye Alev Akçay ◽  
Ayşe Avcı
Keyword(s):  
Yeast Extract ◽  
Protease Activity ◽  
Inoculum Size ◽  
Protease Production ◽  
Bacillus Sp ◽  
Medium Components ◽  
Dipotassium Phosphate ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Selection Of

In this study, effects of medium components and inoculum size on the protease production by Bacillus sp. EBTA6 that was isolated from a home-made Tarhana sample were investigated. The cell-free supernatant of bacterium cultured on a shaking incubator for 24 h was used to test protease activity as the response. With a total number of 11 factors, 12 different experiments were run and the highest experimental protease activity was measured as 2280.4 U/mL. Results were analyzed statistically by ANOVA and the most efficient factors were detected as yeast extract, dipotassium phosphate, casein, and peptone with a contribution of 93.78, 2.19, 1.96, 1.31%, respectively. For validation of the selected factors, a further experiment was performed by using of yeast extract (9.98 g/L), dipotassium phosphate (1.27 g/L), casein (8.69 g/L), and peptone (9.88 g/L) obtained from the design equation. The experimental response was found as 2411.4 U/mL which was only 5.5% higher than the predicted response showing that the model was applicable.

scholarly journals Rapid Evaluation and Optimization of Medium Components Governing Tryptophan Production by Pediococcus acidilactici TP-6 Isolated from Malaysian Food via Statistical Approaches

Molecules ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 779 ◽  
Author(s):  
Ye Heng Lim ◽  
Hooi Ling Foo ◽  
Teck Chwen Loh ◽  
Rosfarizan Mohamad ◽  
Raha Abdul Rahim
Keyword(s):  
Medium Composition ◽  
Rapid Evaluation ◽  
Pediococcus Acidilactici ◽  
Meat Extract ◽  
Medium Components ◽  
Burman Design ◽  
Statistical Approaches ◽  
De Man ◽  
Plackett Burman Design ◽  
Central Composite

Tryptophan is one of the most extensively used amino acids in livestock industry owing to its effectiveness in enhancing the growth performance of animals. Conventionally, the production of tryptophan relies heavily on genetically modified Escherichia coli but its pathogenicity is a great concern. Our recent study demonstrated that a lactic acid bacterium (LAB), Pediococcus acidilactici TP-6 that isolated from Malaysian food was a promising tryptophan producer. However, the tryptophan production must enhance further for viable industrial application. Hence, the current study evaluated the effects of medium components and optimized the medium composition for tryptophan production by P. acidilactici TP-6 statistically using Plackett-Burman Design, and Central Composite Design. The optimized medium containing molasses (14.06 g/L), meat extract (23.68 g/L), urea (5.56 g/L) and FeSO4 (0.024 g/L) significantly enhanced the tryptophan production by 150% as compared to the control de Man, Rogosa and Sharpe medium. The findings obtained in this study revealed that rapid evaluation and effective optimization of medium composition governing tryptophan production by P. acidilactici TP-6 were feasible via statistical approaches. Additionally, the current findings reveal the potential of utilizing LAB as a safer alternative tryptophan producer and provides insight for future exploitation of various amino acid productions by LAB.

scholarly journals Optimization of Extraction or Purification Process of Multiple Components from Natural Products: Entropy Weight Method Combined with Plackett–Burman Design and Central Composite Design

Molecules ◽  
2021 ◽  
Vol 26 (18) ◽  
pp. 5572
Author(s):  
Yu Du ◽  
Pengcheng Huang ◽  
Weifeng Jin ◽  
Chang Li ◽  
Jiehong Yang ◽  
...  
Keyword(s):  
Natural Products ◽  
Purification Process ◽  
Entropy Weight ◽  
Multiple Components ◽  
Entropy Weight Method ◽  
Weight Method ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Central Composite ◽  
Comprehensive Score

In this paper, the optimization of the extraction/purification process of multiple components was performed by the entropy weight method (EWM) combined with Plackett–Burman design (PBD) and central composite design (CCD). We took the macroporous resin purification of Astragalus saponins as an example to discuss the practicability of this method. Firstly, the weight of each component was given by EWM and the sum of the product between the componential content and its weight was defined as the comprehensive score, which was taken as the evaluation index. Then, the single factor method was adopted for determining the value range of each factor. PBD was applied for screening the significant factors. Important variables were further optimized by CCD to determine the optimal process parameters. After the combination of EWM, PBD and CCD, the resulting optimal purification conditions were as follows: pH value of 6.0, the extraction solvent concentration of 0.15 g/mL, and the ethanol volume fraction of 75%. Under the optimal conditions, the practical comprehensive score of recoveries of saponins was close to the predicted value (n = 3). Therefore, the present study provided a convenient and efficient method for extraction and purification optimization technology of multiple components from natural products.

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