Long term storage of callus cultures at low temperatures or under mineral oil layer

1986 ◽  
Vol 5 (5) ◽  
pp. 372-376 ◽  
Author(s):  
J. M. Augereau ◽  
D. Courtois ◽  
V. Petiard
Keyword(s):  
Low Temperatures ◽  
Mineral Oil ◽  
Callus Cultures ◽  
Long Term Storage ◽  
Term Storage

Patterns of lichen photosynthesis and respiration following prolonged frozen storage

1978 ◽  
Vol 56 (17) ◽  
pp. 2119-2123 ◽  
Author(s):  
Douglas W. Larson
Keyword(s):  
Light Intensity ◽  
Low Temperatures ◽  
Physiological Activity ◽  
Frozen Storage ◽  
Experimental Systems ◽  
Long Term Storage ◽  
Photosynthesis And Respiration ◽  
Term Storage ◽  
Respiratory Responses

The effect of prolonged frozen storage on patterns of photosynthesis and respiration in the lichen Alectoria ochroleuca (Hoffm.) Massal. has been examined. The results indicate that this plant not only survives long-term exposure to low temperatures but also that its basic photosynthetic and respiratory responses to temperature, light intensity, and thallus moisture content are altered very little by long-term storage at −60 °C. This maintenance not only of absolute viability but also of the more subtle patterns of physiological activity suggests that such storage may be used to hold lichen material for use in multivariate experimental systems which require replicates having identical field pretreatment.

scholarly journals Selected cryopreservatives for long term storage of Helicobacter pylori at low temperatures.

1992 ◽  
Vol 45 (8) ◽  
pp. 735-736 ◽  
Author(s):  
M Shahamat ◽  
C Paszko-Kolva ◽  
U E Mai ◽  
H Yamamoto ◽  
R R Colwell
Keyword(s):  
Helicobacter Pylori ◽  
Low Temperatures ◽  
Long Term Storage ◽  
Term Storage

Long-term storage of blood samples as whole blood at extremely low temperatures for methemoglobin determination

1988 ◽  
Vol 37 (2) ◽  
pp. 99-104 ◽  
Author(s):  
Keizo Sato ◽  
Keiji Tamaki ◽  
Hiroshi Okajima ◽  
Yoshinao Katsumata
Keyword(s):  
Whole Blood ◽  
Low Temperatures ◽  
Blood Samples ◽  
Long Term Storage ◽  
Term Storage

scholarly journals A Study on the Combination of Enzyme Stabilizers and Low Temperatures in the Long-Term Storage of Glutamate Biosensor

Chemosensors ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 129
Author(s):  
Andrea Bacciu ◽  
Paola Arrigo ◽  
Rossana Migheli ◽  
Alessandra T. Peana ◽  
Gaia Rocchitta ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Low Temperatures ◽  
Triethylene Glycol ◽  
Storage Conditions ◽  
Low Temperature Storage ◽  
Long Term Storage ◽  
The Impact ◽  
Term Storage

The importance of physiological glutamate has been widely demonstrated in cognitive and memory processes, as well as in neurotransmission. The involvement of physiological glutamate in several pathologies has also been established. Therefore, analytical devices for studying variations in physiological glutamate are of fundamental importance, particularly in preclinical studies. The necessary knowledge to develop and characterize biosensors for glutamate detection is often restricted to only a few research groups. However, many more groups have sought to implant such analytical devices to study the glutamatergic system in vivo. On this basis, a series of studies was undertaken to explore the medium-term storage of biosensors, thereby allowing their usage results to be differentiated from their construction and characterization processes to facilitate the wider diffusion and use of such sensors. Therefore, it has become vital to determine the best storage conditions to extend the life and functionality of these biosensors, especially due to the diachronic instability of the enzyme present on the surface. In the present study, we analyzed the impact of glycols, such as glycerol and triethylene glycol, as enzyme stabilizers coupled with long-term storage at low temperatures (−20 and −80 °C) on biosensor performance. The biosensors were observed for 5 months and evaluated for their enzymatic activity by measuring the VMAX(app) and KM(app). The analytical features were also evaluated in terms of the Linear Region Slope, which is one the most important parameters for indicating the efficiency and the sensitivity of biosensors. Interestingly, both glycols proved to be capable of increasing enzymatic activity and maintaining good biosensor efficiency over time. Moreover, the combination with low-temperature storage highlighted the different behaviors of the two glycols. In particular, glycerol was more effective in stabilizing the enzyme and maintaining analytical performance when the biosensors were stored at −20 °C. Instead, triethylene glycol performed the same function as glycerol but when the biosensors were stored at −80 °C.

Survival of wood-inhabiting fungi stored for 10 years in water and under oil

1992 ◽  
Vol 38 (8) ◽  
pp. 861-864 ◽  
Author(s):  
Gary C. Johnson ◽  
Andrew K. Martin
Keyword(s):  
Key Words ◽  
Water Storage ◽  
Mineral Oil ◽  
Sterile Water ◽  
Long Term Storage ◽  
Oil Storage ◽  
Term Storage ◽  
Water Survival

Subcultures of 1064 isolates of 393 species of wood-inhabiting fungi were stored both under sterile mineral oil and in sterile water for 10 years. A high proportion (98%) of the Basidiomycotina isolates survived storage under oil, whereas only 26% survived in sterile water. Survival of most isolates of Ascomycotina (91% under oil, 72% in water) and Deuteromycotina (96% under oil, 92% in water) was somewhat less in water than under oil. Key words: fungal storage, long-term storage, oil storage, water storage, Basidiomycotina.

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