THE VITAMIN REQUIREMENTS OF CORYNEBACTERIUM SEPEDONICUM (SPIEK. & KOTT.) SKAPT. & BURKH.

1960 ◽  
Vol 6 (2) ◽  
pp. 171-181 ◽  
Author(s):  
Robert-A. Lachance
Keyword(s):  
Nicotinic Acid ◽  
Free Acid ◽  
Tween 80 ◽  
Pimelic Acid ◽  
Mineral Salts ◽  
Corynebacterium Sepedonicum ◽  
Casein Hydrolyzate ◽  
Vitamin Requirements

The vitamin requirements of Corynebacterium sepedonicum (Spiek. & Kott.) Skapt. & Burkholder were studied in a medium containing vitamin-free acid casein hydrolyzate, glucose, and mineral salts. Nicotinic acid, thiamine, and biotin were found to be stimulatory. Riboflavin inhibited the growth slightly. Nicotinic acid can be replaced by nicotinamide, but Tween 80 and pimelic acid could not replace biotin.

THE AMINO-ACID REQUIREMENTS OF CORYNEBACTERIUM SEPEDONICUM (SPIEK. & KOTT.) SKAPT. & BURKH.

1962 ◽  
Vol 8 (3) ◽  
pp. 321-325 ◽  
Author(s):  
Robert-A. Lachance
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Mineral Salts ◽  
Specific Amino Acid ◽  
Corynebacterium Sepedonicum ◽  
Casein Hydrolyzate ◽  
Absolute Requirement ◽  
Nutritional Importance ◽  
Amino Acid Requirements

The amino-acid requirements of Corynebacterium sepedonicum (Spiek. & Kott.) Skapt. & Burkh. were studied in a medium containing vitamins, glucose, and mineral salts. Seven amino acids, asparagine, methionine, leucine, histidine, alanine, arginine, and proline, were found to be required to obtain growth equivalent to that obtained with casein hydrolyzate, though asparagine and methionine are of major nutritional importance: no absolute requirement for a specific amino acid could be demonstrated. Tryptophane, tyrosine, phenylalanine, cystine, and cysteine were found to be inhibitory.

scholarly journals Detection and Characterization of a Nicotinic Acid-Replacing Substance in Vitamin-Free Acid Hydrolyzed Casein

1983 ◽  
Vol 66 (4) ◽  
pp. 743-749 ◽  
Author(s):  
T.J. Kerr ◽  
M.V. Williams ◽  
J.J. Rowe ◽  
G.J. Tritz ◽  
C.J. Washam
Keyword(s):  
Nicotinic Acid ◽  
Free Acid

scholarly journals Intracellular symbiosis and vitamin requirements of two insects, Lasioderma serricorne and Sitodrepa panicea

1944 ◽  
Vol 132 (867) ◽  
pp. 212-221 ◽  
Keyword(s):  
Growth Rate ◽  
Nicotinic Acid ◽  
Artificial Diet ◽  
Pantothenic Acid ◽  
Lasioderma Serricorne ◽  
White Flour ◽  
Vitamin Requirements ◽  
Pure Substances ◽  
Better Than

Normal larvae of two beetles, Lasioderma serricorne and Sitodrepa panicea , and larvae freed from their intracellular symbionts have been grown on various diets. On white flour, a food deficient in vitamins of the B group, normal larvae grow very much better than sterilized larvae, while no such difference in the growth rate occurs on a diet rich in vitamins of the B group, e. g. wholemeal flour plus yeast. On an artificial diet which contains the vitamins of the B complex in pure substances, the normal Lasioderma larva grows well or fairly well in the absence of either thiamin, riboflavin, nicotinic acid, pyridoxin or pantothenic acid, and the normal Sitodrepa larva in the absence of any of these vitamins except thiamin. The sterilized larvae of both species fail to grow in the absence of any of these five vitamins. It is concluded that the intracellular symbionts of Lasioderma and Sitodrepa supply vitamins of the B group.

scholarly journals Pentachlorophenol Degradation byJanibactersp., a New Actinobacterium Isolated from Saline Sediment of Arid Land

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Amel Khessairi ◽  
Imene Fhoula ◽  
Atef Jaouani ◽  
Yousra Turki ◽  
Ameur Cherif ◽  
...  
Keyword(s):  
Biological Treatment ◽  
High Performance ◽  
Gene Sequence ◽  
Elevated Temperatures ◽  
Tween 80 ◽  
Mineral Salts ◽  
Removal Capacity ◽  
Degrading Bacterium ◽  
Gene Sequence Analysis ◽  
Extreme Habitats

Many pentachlorophenol- (PCP-) contaminated environments are characterized by low or elevated temperatures, acidic or alkaline pH, and high salt concentrations. PCP-degrading microorganisms, adapted to grow and prosper in these environments, play an important role in the biological treatment of polluted extreme habitats. A PCP-degrading bacterium was isolated and characterized from arid and saline soil in southern Tunisia and was enriched in mineral salts medium supplemented with PCP as source of carbon and energy. Based on 16S rRNA coding gene sequence analysis, the strain FAS23 was identified asJanibactersp. As revealed by high performance liquid chromatography (HPLC) analysis, FAS23 strain was found to be efficient for PCP removal in the presence of 1% of glucose. The conditions of growth and PCP removal by FAS23 strain were found to be optimal in neutral pH and at a temperature of 30°C. Moreover, this strain was found to be halotolerant at a range of 1–10% of NaCl and able to degrade PCP at a concentration up to 300 mg/L, while the addition of nonionic surfactant (Tween 80) enhanced the PCP removal capacity.

Selective medium for the isolation and enumeration of Mycobacterium avium-intracellulare and M. scrofulaceum

1986 ◽  
Vol 32 (1) ◽  
pp. 10-14 ◽  
Author(s):  
Karen L. George ◽  
Joseph O. Falkinham III
Keyword(s):  
Carbon Source ◽  
Mycobacterium Avium ◽  
Sole Carbon Source ◽  
Natural Waters ◽  
Tween 80 ◽  
Selective Medium ◽  
Selective Isolation ◽  
Mineral Salts ◽  
Mycobacterium Avium Intracellulare

A medium for the selective isolation and enumeration of Mycobacterium avium-intracellulare and M. scrofulaceum (MAIS) was developed, based upon the ability of these mycobacteria to utilize Tween 80 as sole carbon source and grow optimally at pH 5.5 on a simple mineral salts medium. Representative MAIS strains had higher efficiencies of plating on the Tween 80 medium compared with Middlebrook 7H10. It was shown that nonmycobacterial organisms in natural waters had lower efficiencies of plating on the Tween 80 medium and smaller colonies, thus allowing direct isolation and enumeration of the slowly growing mycobacteria without overgrowth.

Effets des purines et des pyrimidines sur la croissance de Corynebacterium sepedonicum (Spiek. & Kott.) Skapt. & Burkh.

1968 ◽  
Vol 14 (4) ◽  
pp. 475-478 ◽  
Author(s):  
Lucien M. Bordeleau ◽  
Robert-A. Lachance
Keyword(s):  
Amino Acids ◽  
Nucleic Acids ◽  
Synthetic Medium ◽  
Limiting Factor ◽  
Mineral Salts ◽  
Corynebacterium Sepedonicum ◽  
Purines And Pyrimidines

The effects of purines and pyrimidines on the growth of Corynebacterium sepedonicum were studied in a synthetic medium containing amino acids, vitamins, mineral salts, and glucose. Among four purines tested, only adenine inhibited the growth of the organism. Hypoxanthine and guanine reversed the inhibition caused by adenine. None of four pyrimidines tested affected the growth. In the presence of pyrimidines, only hypoxanthine reversed the inhibition caused by adenine. It appears that purines and pyrimidines do not stimulate the growth of C. sepedonicum and that the synthesis of nucleic acids from these bases is not a limiting factor in the growth of the organism.

Vitamin requirements of the Coccidia of the chicken

Parasitology ◽  
1968 ◽  
Vol 58 (1) ◽  
pp. 137-148 ◽  
Author(s):  
E. W. Warren
Keyword(s):  
Folic Acid ◽  
Nicotinic Acid ◽  
Normal Development ◽  
Eimeria Acervulina ◽  
Vitamin Requirements

For normal development of the parasites, Eimeria acervulina and E. tenella in the chicken, dietary thiamine, riboflavin, biotin, nicotinic acid and folic acid are required. E. acervulina required thiamine for second schizogony and sporulation; riboflavin for first schizogony and possibly gametogony, biotin for the development of the sporozoites/trophozoites and for gametogony, and nicotinic acid for schizogony and gametogony. E. tenella required thiamine and riboflavin for gametogony; biotin for first schizogony; and nicotinic acid for second schizogony.

NITROGEN AND VITAMIN REQUIREMENTS OF PSEUDOMONAS HYDROPHILA

1953 ◽  
Vol 31 (2) ◽  
pp. 206-211 ◽  
Author(s):  
Florence R. Tamboline
Keyword(s):  
Yeast Extract ◽  
Synthetic Medium ◽  
Pantothenic Acid ◽  
Ammonium Phosphate ◽  
Ammonium Salts ◽  
Ammonium Citrate ◽  
Mineral Salts ◽  
Vitamin Requirements ◽  
Casamino Acids ◽  
Simple Synthetic Medium

Yeast extract and vitamin-free casamino acids were found to be equivalent as sources of nitrogen for the growth of Pseudomonas hydrophila in a glucose – mineral salts medium. The addition of a mixture of thiamine, riboflavin, pantothenic acid, pyridoxine, nicotinic acid, para-aminobenzoic acid, biotin, and folic acid to the medium containing vitamin-free casamino acids did not stimulate growth. About 67% as much growth was obtained with a mixture of 20 amino acids and asparagine as with the vitamin-free casamino acids and the mixture could be replaced by any one of asparagine, aspartic acid, serine, and alanine. Of the 11 simple nitrogen compounds tested, including urea, nitrates, and ammonium salts, only ammonium citrate and dibasic ammonium phosphate were utilized appreciably. A simple synthetic medium consisting of ammonium citrate, glucose, and mineral salts was found to give approximately the same amount of growth as the more complex yeast extract – glucose – mineral salts medium.

VITAMIN REQUIREMENTS OF A LOW-TEMPERATURE BASIDIOMYCETE

1962 ◽  
Vol 40 (10) ◽  
pp. 1347-1354 ◽  
Author(s):  
E. W. B. Ward
Keyword(s):  
Low Temperature ◽  
Optimum Concentration ◽  
Western Canada ◽  
Shake Culture ◽  
Optimum Growth ◽  
Mineral Salts ◽  
Snow Mold ◽  
Absolute Requirement ◽  
Vitamin Requirements

The vitamin requirements of an unidentified basidiomycete, the cause of severe snow mold of grasses and legumes in Western Canada, have been determined in shake culture. In a medium composed of D-glucose, L-asparagine, and mineral salts, a mixture of several vitamins supported optimum growth, but an absolute requirement was demonstrated only for thiamine. Pyridoxine stimulated growth in the presence of thiamine. The optimum concentration of thiamine was 50 μg per liter and below this level growth was proportional to concentration. Pyridoxine was effective in the range 50–100 μg per liter in the presence of 50 μg per liter of thiamine. The requirement for thiamine could be satisfied by a mixture of equivalent amounts of thiazole and pyrimidine but not by either alone.

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