CENTROMERIC BANDING IN MAIZE

1981 ◽  
Vol 23 (2) ◽  
pp. 255-258 ◽  
Author(s):  
C. Chow ◽  
E. N. Larter
Keyword(s):  
Zea Mays L ◽  
Banding Technique ◽  
Treatment Schedule ◽  
Positive Contribution ◽  
Salt Treatment ◽  
Metaphase Chromosomes ◽  
C Banding ◽  
Combined Action ◽  
Complete Set ◽  
Water Rinse

A modified Leishman C-banding technique is described which can produce a complete set of centromeric bands on the somatic metaphase chromosomes of maize (Zea mays L.). Several interstitial (minor) C-bands, additional to those revealed by the conventional C-banding method, were observed. Centromeric bands were produced only after both an alkali and salt treatment were incorporated into the treatment schedule and a subsequent water rinse was omitted. Apparently, the combined action of alkali and salt treatments provided a positive contribution to the centromeric regions which otherwise remained unstained using the standard C-banding technique.

DIFFERENTIAL GIEMSA STAINING IN PLANTS. VI. CENTROMERIC BANDING

1979 ◽  
Vol 21 (3) ◽  
pp. 373-378 ◽  
Author(s):  
W. Gary Filion ◽  
David H. Blakey
Keyword(s):  
Room Temperature ◽  
Chromosome Banding ◽  
Giemsa Staining ◽  
Barium Hydroxide ◽  
Banding Technique ◽  
Giemsa Banding ◽  
Metaphase Chromosomes ◽  
Somatic Metaphase ◽  
C Banding ◽  
Hydrolysis Temperature

Somatic metaphase chromosomes of Tulipa which were subjected to various hydrolyses with several times and temperatures displayed two distinctive types of C-banding when stained using the BSG (Barium hydroxide/Saline/Giemsa) chromosome banding technique. In addition to the two types of Giemsa bands, namely intercalary/terminal and centromeric, a unique transition from the former to the latter type of banding was observed. That is, at the point of transition from intercalary/terminal to centromeric banding, both types were present at one time. The two types of Giemsa banding resulted from different HCl hydrolysis times and temperatures; centromeric bands being observed after either a prolonged hydrolysis at room temperature or an increase in the hydrolysis temperature to 60 °C. These results are discussed in relation to the mechanisms of chromosome banding.

Cytogenetic identification of Triticum peregrinum chromosomes added to common wheat

Genome ◽  
1996 ◽  
Vol 39 (2) ◽  
pp. 272-276 ◽  
Author(s):  
B. Friebe ◽  
E. D. Badaeva ◽  
B. S. Gill ◽  
N. A. Tuleen
Keyword(s):  
Triticum Aestivum ◽  
Key Words ◽  
Common Wheat ◽  
Addition Lines ◽  
Karyotypic Analysis ◽  
Chromosome Addition ◽  
C Banding ◽  
Chromosome Addition Lines ◽  
Complete Set ◽  
Donor Species

C-banded karyotypes of a complete set of 14 Triticum peregrinum whole chromosome addition lines and 25 telosomic addition lines are reported. The added T. peregrinum chromosomes were not structurally rearranged compared with the corresponding chromosomes of the donor accession. Comprehensive karyotypic analysis confirmed Triticum umbellulatum as the donor species of the Uv genome and identified Triticum longissimum as the donor species of the Sv genome of T. peregrinum. Neither the Uv nor Sv genome chromosomes of the T. peregrinum accession showed large modifications when compared with the ancestral U and S1 genomes. Key words : Triticum aestivum, Triticum peregrinum, Triticum umbellulatum, Triticum longissimum, chromosome addition lines, C-banding.

Banding of rye (Secale cereale) chromosomes using the restriction enzymes AluI, DraI, HpaII, and MspI

Genome ◽  
1993 ◽  
Vol 36 (5) ◽  
pp. 998-1002 ◽  
Author(s):  
T. Stößer ◽  
T. Günther ◽  
C. U. Hesemann
Keyword(s):  
Secale Cereale ◽  
Restriction Enzymes ◽  
Chromosome Band ◽  
Banding Technique ◽  
Recognition Sequence ◽  
Metaphase Chromosomes ◽  
Banding Patterns ◽  
Secale Cereale L ◽  
Characteristic Banding

Mitotic metaphase chromosomes of the rye inbred line L 301, which belongs to the Sortiment of the University of Hohenheim, were treated in situ with the restriction enzymes AluI (recognition sequence: 5′-AC/GT-3′), DraI (recognition sequence: 5′-TTT/AAA-3′), and the isoschizomeres HpaII and MspI (recognition sequence: 5′-C/CGG-3′) and stained with Giemsa. The chromosomes indicated similar banding patterns in comparison with the conventional Giemsa-C-banding. However, we have found in rye chromosomes after restrictase treatment that the telomeric bands were reduced in extension. In a lower degree the centromeric bands of individual chromosomes could be absent in dependence of the used restriction enzymes. The number of the intercalary bands were also reduced. Nevertheless, the tested restriction enzymes produced characteristic banding patterns of the rye genome. This uncomplicated banding technique is suited for a very quick banding method of karyotype analysis especially to obtain a first survey of the band patterns on the rye chromosomes.Key words: Secale cereale L., chromosome band pattern, in situ digestion, restriction endonuclease, restriction banding.

scholarly journals NaCl stress causes changes in photosynthetic pigments and accumulation of compatible solutes in Zea mays L.

2016 ◽  
Vol 1 (1) ◽  
pp. 3 ◽  
Author(s):  
Rajendran Sozharajan ◽  
Sabanayagam Natarajan
Keyword(s):  
Zea Mays ◽  
Photosynthetic Pigments ◽  
Zea Mays L ◽  
Block Design ◽  
Photosynthetic Pigment ◽  
Compatible Solutes ◽  
Nacl Stress ◽  
Salt Treatment ◽  
Randomized Block Design ◽  
Photosynthetic Pigment Content

The present study was conducted in order to evaluate the effects of NaCl on photosynthetic pigments and compatible solutes of Zea mays under salt stress. Seven NaCl regimes were used, 0mM, 25mM, 50mM, 75mM 100mM, 125mM and 150mM. Plants were analyzed on 15th day after salt treatment. A factorial experiment in a completely randomized block design (CRBD) with seven treatments and three replications were applied. From the data attained, we understand that in accordance with the increase in salinity, photosynthetic pigment content reduced drastically, whereas compatible solutes like proline, glycine betaine and sugar enhanced marginally. The accumulation of compatible solutes makes the plant survive against salinity stress.

Standard Giemsa C-banded karyotype of Russian wildrye (Psathyrostachys juncea) and its use in identification of a deletion–translocation heterozygote

Genome ◽  
1995 ◽  
Vol 38 (6) ◽  
pp. 1262-1270 ◽  
Author(s):  
Jun-Zhi Wei ◽  
William F. Campbell ◽  
Richard R.-C. Wang
Keyword(s):  
Banding Pattern ◽  
Geographic Area ◽  
B Chromosome ◽  
Distal Segment ◽  
Banding Technique ◽  
Translocation Heterozygote ◽  
C Banding ◽  
Psathyrostachys Juncea ◽  
Geographical Regions ◽  
Russian Wildrye

Ten accessions of Russian wildrye, Psathyrostachys juncea (Fisch.) Nevski (2n = 2x = 14; NsNs), collected from different geographical regions were analyzed using the C-banding technique. C-banding pattern polymorphisms were observed at all levels, i.e., within homologous chromosome pairs of the same plant, among different individuals within accessions, between different accessions of the same geographic area, and among accessions of different origins. The seven homologous groups varied in the level of C-banding pattern polymorphism; chromosomes A, B, E, and F were more variable than chromosomes C, D, and G. The polymorphisms did not hamper chromosome identification in Ps. juncea, because each chromosome pair of the Ns genome had a different basic C-banding pattern and karyotypic character. A standard C-banded karyotype of Ps. juncea is proposed based on the overall karyotypes and C-bands in the 10 accessions. The C-bands on the Ns-genome chromosomes were designated according to the rules of nomenclature used in wheat. A deletion–translocation heterozygote of Russian wildrye was identified based on the karyotype and C-banding patterns established. The chromosome F pair consisted of a chromosome having the distal segment in the long arm deleted and a translocated chromosome having the distal segment of long arm replaced by the distal segment of the long arm of chromosome E. The chromosome E pair had a normal chromosome E and a translocated chromosome having the short arm and the proximal segment of the long arm of chromosome E and the distal segment of the long arm of chromosome F.Key words: Psathyrostachys juncea, karyotype, Giemsa C-banding, polymorphism, B chromosome.

Analysis of intraspecific diversity of cultivated emmer Triticum dicoccum (Schrank.) schuebl using C-banding technique

2007 ◽  
Vol 43 (11) ◽  
pp. 1271-1285 ◽  
Author(s):  
O. S. Dedkova ◽  
E. D. Badaeva ◽  
O. P. Mitrofanova ◽  
E. N. Bilinskaya ◽  
V. A. Pukhalskiy
Keyword(s):  
Intraspecific Diversity ◽  
Banding Technique ◽  
Triticum Dicoccum ◽  
C Banding

Rye–wheat hybrids: the production of wheat chromosome additions to rye

Genome ◽  
1991 ◽  
Vol 34 (5) ◽  
pp. 840-844 ◽  
Author(s):  
M. Baum
Keyword(s):  
Wheat Chromosome ◽  
Banding Technique ◽  
Addition Lines ◽  
C Banding ◽  
Good Potential ◽  
Wheat Hybrids

To produce rye–wheat addition lines, 21-chromosome rye–wheat hybrids were produced by crossing tetraploid triticale with diploid rye. The subsequent selfing of the hybrids lead to monosomic and double monosomic wheat additions to rye. Screening of the progeny was carried out using the C-banding technique. The wheat additions can be fertile. Wheat chromosome 6B was the chromosome most frequently added to rye. Ribosomal spacer probe pTa250.4 was used to confirm the results obtained by C-banding for the 6B wheat additions to rye. Embryos of the 21-chromosome rye–wheat hybrids showed a good potential for propagating more plantlets after they had been transferred to artificial medium.Key words: rye–wheat addition lines.

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