Rye–wheat hybrids: the production of wheat chromosome additions to rye

Genome ◽  
1991 ◽  
Vol 34 (5) ◽  
pp. 840-844 ◽  
Author(s):  
M. Baum
Keyword(s):  
Wheat Chromosome ◽  
Banding Technique ◽  
Addition Lines ◽  
C Banding ◽  
Good Potential ◽  
Wheat Hybrids

To produce rye–wheat addition lines, 21-chromosome rye–wheat hybrids were produced by crossing tetraploid triticale with diploid rye. The subsequent selfing of the hybrids lead to monosomic and double monosomic wheat additions to rye. Screening of the progeny was carried out using the C-banding technique. The wheat additions can be fertile. Wheat chromosome 6B was the chromosome most frequently added to rye. Ribosomal spacer probe pTa250.4 was used to confirm the results obtained by C-banding for the 6B wheat additions to rye. Embryos of the 21-chromosome rye–wheat hybrids showed a good potential for propagating more plantlets after they had been transferred to artificial medium.Key words: rye–wheat addition lines.

Cytogenetic identification of Triticum peregrinum chromosomes added to common wheat

Genome ◽  
1996 ◽  
Vol 39 (2) ◽  
pp. 272-276 ◽  
Author(s):  
B. Friebe ◽  
E. D. Badaeva ◽  
B. S. Gill ◽  
N. A. Tuleen
Keyword(s):  
Triticum Aestivum ◽  
Key Words ◽  
Common Wheat ◽  
Addition Lines ◽  
Karyotypic Analysis ◽  
Chromosome Addition ◽  
C Banding ◽  
Chromosome Addition Lines ◽  
Complete Set ◽  
Donor Species

C-banded karyotypes of a complete set of 14 Triticum peregrinum whole chromosome addition lines and 25 telosomic addition lines are reported. The added T. peregrinum chromosomes were not structurally rearranged compared with the corresponding chromosomes of the donor accession. Comprehensive karyotypic analysis confirmed Triticum umbellulatum as the donor species of the Uv genome and identified Triticum longissimum as the donor species of the Sv genome of T. peregrinum. Neither the Uv nor Sv genome chromosomes of the T. peregrinum accession showed large modifications when compared with the ancestral U and S1 genomes. Key words : Triticum aestivum, Triticum peregrinum, Triticum umbellulatum, Triticum longissimum, chromosome addition lines, C-banding.

Characterization of Thinopyrum distichum chromosomes using double fluorescence in situ hybridization, RFLP analysis of 5S and 26S rRNA, and C-banding of parents and addition lines

Genome ◽  
1997 ◽  
Vol 40 (5) ◽  
pp. 689-696 ◽  
Author(s):  
A Fominaya ◽  
S. Molnar ◽  
G. Fedak ◽  
K. C. Armstrong ◽  
N.-S. Kim ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Chromosome Pair ◽  
Triticum Turgidum ◽  
5S Rrna ◽  
Polymorphism Analysis ◽  
Addition Lines ◽  
C Banding ◽  
26S Rrna

Diagnostic markers for eight Thinopyrum distichum addition chromosomes in Triticum turgidum were established using C-banding, in situ hybridization, and restriction fragment length polymorphism analysis. The C-band karyotype conclusively identified individual Th. distichum chromosomes and distinguished them from chromosomes of T. turgidum. Also, TaqI and BamHI restriction fragments containing 5S and 18S–5.8S–26S rRNA sequences were identified as positive markers specific to Th. distichum chromosomes. Simultaneous fluorescence in situ hybridization showed both 5S and 18S–5.8S–26S ribosomal RNA genes to be located on chromosome IV. Thinopyrum distichum chromosome VII carried only a 18S–5.8S–26S rRNA locus and chromosome pair II carried only a 5S rRNA locus. The arrangement of these loci on Th. distichum chromosome IV was different from that on wheat chromosome pair 1B. Two other unidentified Th. distichum chromosome pairs also carried 5S rRNA loci. The homoeologous relationship between Th. distichum chromosomes IV and VII and chromosomes of other members of the Triticeae was discussed by comparing results obtained using these physical and molecular markers.Key words: Triticum turgidum, homoeologous relationship, Triticeae, addition lines, NOR.

Meiotic pairing in wheat–rye addition and substitution lines

1984 ◽  
Vol 26 (1) ◽  
pp. 25-33 ◽  
Author(s):  
J. Orellana ◽  
M. C. Cermeño ◽  
J. R. Lacadena
Keyword(s):  
Chromosome Pairing ◽  
Chinese Spring ◽  
Constitutive Heterochromatin ◽  
Banding Technique ◽  
Addition Line ◽  
Meiotic Pairing ◽  
Addition Lines ◽  
Substitution Lines ◽  
Homologous Chromosomes ◽  
Complex Process

Chromosome pairing was examined in wheat–rye addition and substitution lines using the C-banding technique. It was found that both rye and wheat chromosomes affect each other's homologous pairing. The strongest diminution of wheat pairing (measured as bound arms per cell) was produced by chromosome 5R of rye (7.5 and 7.2% in 'Chinese Spring' – 'Imperial' and 'Holdfast' – 'King II' addition lines, respectively). The weakest diminution of wheat pairing was produced by chromosome 3R in the 'Chinese Spring' – 'Imperial' addition line (1.1%). The diminution of rye chromosome pairing produced by wheat chromosomes ranges from 6.9 to 48.4% ('Chinese Spring' – 'Imperial' and 'Holdfast' – 'King II' addition lines, respectively). When put into a wheat background, the rye chromosomes suffer a worse fate than the wheat chromosomes. For example, chromosome 6R reduces the wheat complement pairing in the 'Holdfast' – 'King II' addition line by 3.8% but its own pairing is reduced by 41.4%. The decrease in pairing of both wheat and rye homologous chromosomes in addition and substitution lines is a complex process in which factors such as genes controlling meiotic pairing, constitutive heterochromatin, and cryptic wheat–rye interactions can play important roles.

Gliadin gene location and C-banding identification of Aegilops longissima chromosomes added to wheat

Genome ◽  
1991 ◽  
Vol 34 (2) ◽  
pp. 236-240 ◽  
Author(s):  
G. Hueros ◽  
J. M. Gonzalez ◽  
J. C. Sanz ◽  
E. Ferrer
Keyword(s):  
Gene Location ◽  
Addition Lines ◽  
Substitution Lines ◽  
Structural Modifications ◽  
C Banding ◽  
Aegilops Longissima ◽  
Gli 1 ◽  
Protein Components ◽  
Gliadin Protein ◽  
Alien Addition Lines

Gliadin protein components from Aegilops longissima were separated by two-dimensional electrophoresis. No equivalents for α-gliadin were noted. Addition and substitution lines of Ae. longissima in Triticum aestivum 'Chinese Spring' allowed the identification of homoeologous Gli-1 and Gli-2 loci in Ae. longissima chromosomes 1S1 and 6S1. The chromosomal constitution of the alien addition lines was ascertained by C-banding. In addition, C-banding analysis revealed that the Ae. longissima addition set was incomplete as only six distinct addition lines were identified. No evidence for structural modifications between the alien chromosomes in the lines and their Ae. longissima counterparts was found.Key words: gliadins, C-banding, gene location, Aegilops longissima, wheat.

Standard Giemsa C-banded karyotype of Russian wildrye (Psathyrostachys juncea) and its use in identification of a deletion–translocation heterozygote

Genome ◽  
1995 ◽  
Vol 38 (6) ◽  
pp. 1262-1270 ◽  
Author(s):  
Jun-Zhi Wei ◽  
William F. Campbell ◽  
Richard R.-C. Wang
Keyword(s):  
Banding Pattern ◽  
Geographic Area ◽  
B Chromosome ◽  
Distal Segment ◽  
Banding Technique ◽  
Translocation Heterozygote ◽  
C Banding ◽  
Psathyrostachys Juncea ◽  
Geographical Regions ◽  
Russian Wildrye

Ten accessions of Russian wildrye, Psathyrostachys juncea (Fisch.) Nevski (2n = 2x = 14; NsNs), collected from different geographical regions were analyzed using the C-banding technique. C-banding pattern polymorphisms were observed at all levels, i.e., within homologous chromosome pairs of the same plant, among different individuals within accessions, between different accessions of the same geographic area, and among accessions of different origins. The seven homologous groups varied in the level of C-banding pattern polymorphism; chromosomes A, B, E, and F were more variable than chromosomes C, D, and G. The polymorphisms did not hamper chromosome identification in Ps. juncea, because each chromosome pair of the Ns genome had a different basic C-banding pattern and karyotypic character. A standard C-banded karyotype of Ps. juncea is proposed based on the overall karyotypes and C-bands in the 10 accessions. The C-bands on the Ns-genome chromosomes were designated according to the rules of nomenclature used in wheat. A deletion–translocation heterozygote of Russian wildrye was identified based on the karyotype and C-banding patterns established. The chromosome F pair consisted of a chromosome having the distal segment in the long arm deleted and a translocated chromosome having the distal segment of long arm replaced by the distal segment of the long arm of chromosome E. The chromosome E pair had a normal chromosome E and a translocated chromosome having the short arm and the proximal segment of the long arm of chromosome E and the distal segment of the long arm of chromosome F.Key words: Psathyrostachys juncea, karyotype, Giemsa C-banding, polymorphism, B chromosome.

Characterization of an Agropyron elongatum chromosome conferring resistance to cephalosporium stripe in common wheat

Genome ◽  
1996 ◽  
Vol 39 (1) ◽  
pp. 56-62 ◽  
Author(s):  
Xiwen Cai ◽  
Stephen S. Jones ◽  
Timothy D. Murray
Keyword(s):  
In Situ Hybridization ◽  
Wheat Chromosome ◽  
Triticum Aestivum L ◽  
Chromosome Substitution ◽  
Agropyron Elongatum ◽  
Breeding Lines ◽  
C Banding ◽  
Small Band ◽  
Cephalosporium Gramineum

Related wheat (Triticum aestivum L.) breeding lines, PI 561033, REA 9232, REA 9257, and CI 13113 were analyzed cytogenetically to characterize the association of resistance to cephalosporium stripe (caused by Cephalosporium gramineum Nis. & Ika.) with Agropyron elongatum chromatin. One pair of A. elongatum chromosomes was detected in PI 561033, REA 9232, and CI 13113 by genomic in situ hybridization. The sib line of PI 561033 and REA 9232, REA 9257, which is not resistant to this disease, lacked this pair of A. elongatum chromosomes. PI 561033 was characterized as a disomic T. aestivum – A. elongatum 6Ae#2(6A) chromosome substitution line using test crosses and C-banding. In situ hybridization and test crosses showed that the donor parent, CI 13113, also had chromosome 6A substituted by A. elongatum chromosome 6Ae#2. The C-banding pattern of 6Ae#2 showed two subterminal bands on the long arm and one small band proximal to the centromere on the short arm. Based on chromosome pairing and compensation, chromosome 6Ae#2 shows a close homoeologous relationship with wheat chromosome 6A. Key words : Cephalosporium gramineum, Agropyron elongatum, in situ hybridization, C-banding, chromosome substitution.

Analysis of intraspecific diversity of cultivated emmer Triticum dicoccum (Schrank.) schuebl using C-banding technique

2007 ◽  
Vol 43 (11) ◽  
pp. 1271-1285 ◽  
Author(s):  
O. S. Dedkova ◽  
E. D. Badaeva ◽  
O. P. Mitrofanova ◽  
E. N. Bilinskaya ◽  
V. A. Pukhalskiy
Keyword(s):  
Intraspecific Diversity ◽  
Banding Technique ◽  
Triticum Dicoccum ◽  
C Banding

Modified Method of C Banding Using Barium Hydroxide

1975 ◽  
Vol 24 (3-4) ◽  
pp. 315-316
Author(s):  
P.K. Ghosh ◽  
Indera P. Singh
Keyword(s):  
Barium Hydroxide ◽  
Centromeric Heterochromatin ◽  
Banding Technique ◽  
C Banding ◽  
Modified Method ◽  
The Relationship

A modified centromeric heterochromatin banding technique using barium hydroxide octahydrate is described. The relationship between slide maturity and time of denaturation by barium-hydroxide is discussed.

scholarly journals Karyotyping the Arabian Camel (Camelus dromedarius), Using C-Banding Technique.

CYTOLOGIA ◽  
1992 ◽  
Vol 57 (3) ◽  
pp. 383-388 ◽  
Author(s):  
Mohammed A. Samman ◽  
Abdulaziz A. Al-Saleh ◽  
Kart Sheth
Keyword(s):  
Camelus Dromedarius ◽  
Banding Technique ◽  
C Banding ◽  
Arabian Camel
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