Efficiency in a forest nursery of an ectomycorrhizal fungus inoculum produced in a fermentor and entrapped in polymeric gels

1985 ◽  
Vol 63 (9) ◽  
pp. 1664-1668 ◽  
Author(s):  
F. Le Tacon ◽  
G. Jung ◽  
J. Mugnier ◽  
P. Michelot ◽  
C. Mauperin
Keyword(s):  
Norway Spruce ◽  
Seedling Growth ◽  
Ectomycorrhizal Fungus ◽  
Douglas Fir ◽  
Forest Nursery ◽  
Thelephora Terrestris ◽  
Hebeloma Cylindrosporum ◽  
Polymeric Gels ◽  
Mycorrhizal Development ◽  
High Metabolic Activity

In a nursery in the centre of France, we have compared the effect of different forms of an ectomycorrhizal fungus inoculum (Hebeloma cylindrosporum) on growth and mycorrhizal development of Douglas-fir and Norway spruce after soil fumigation. Compared with inoculum produced on peat and vermiculite, the inoculum produced in a fermentor and entrapped in polymeric gels significantly improved Douglas-fir and Norway spruce seedling growth. The Hebeloma mycorrhizal index was not significantly different between the different forms of inocula. The superiority of the inoculum produced in the fermentor and entrapped in gels is probably related to the high metabolic activity of the mycelium and to the protection given by the polymers after the incorporation of the inoculum into the soil. Hebeloma cylindrosporum stimulates seedling growth even in the presence of Thelephora terrestris mycorrhiza.

Pure culture synthesis of ectomycorrhizae by Thelephora terrestris and Pisolithus tinctorius on different conifer hosts

1970 ◽  
Vol 48 (3) ◽  
pp. 639-643 ◽  
Author(s):  
Donald H. Marx ◽  
W. Craig Bryan
Keyword(s):  
Plant Growth ◽  
Norway Spruce ◽  
Pure Culture ◽  
Douglas Fir ◽  
Pisolithus Tinctorius ◽  
Thelephora Terrestris ◽  
Fungal Symbiont ◽  
Growth Room ◽  
Pure Culture Synthesis

In a special plant-growth room, isolates of Thelephora terrestris produced basidiocarps and formed typical ectomycorrhizae with seedlings of bristlecone, jack, sand, lodgepole, shortleaf, slash, sugar, Austrian, longleaf, cluster, ponderosa, red, pitch, eastern white, Scots, loblolly, and Virginia pines and Douglas fir. Atypical mycorrhizae (lacking mantle) were formed on seedlings of Norway spruce and jack, Japanese red, and Himalayan pines. The formation of atypical mycorrhizae was considered a result of differences in the symbiotic–parasitic nature of the fungal symbionts on different hosts. Pisolithus tinctorius formed typical mycorrhizae with seedlings of jack, sand, Japanese red, shortleaf, slash, Austrian, longleaf, cluster, red, pitch, eastern white, Scots, loblolly, and Virginia pines. Reisolation of specific fungal symbionts from mycorrhizae of several pine hosts was successful.Mycorrhizae formed by T. terrestris were macroscopically and microscopically different from those of P. tinctorius, but mycorrhizae formed by different isolates of T. terrestris were indistinguishable from each other, regardless of host. These results suggest that the fungal symbiont determines color and morphology of ectomycorrhizae.

Use of the ectomycorrhizal fungus Laccarialaccata in forestry. I. Consistency between isolates in effective colonization of containerized conifer seedlings

1982 ◽  
Vol 12 (3) ◽  
pp. 469-473 ◽  
Author(s):  
Randy Molina
Keyword(s):  
Seedling Growth ◽  
Ponderosa Pine ◽  
Dry Weight ◽  
Sitka Spruce ◽  
Ectomycorrhizal Fungus ◽  
Douglas Fir ◽  
Western Hemlock ◽  
Conifer Species ◽  
Colonization Success ◽  
Inoculation Treatment

Four isolates of the ectomycorrhizal fungus Laccarialaccata (Scop. ex Fr.) Berk and Br. were inoculated singly onto containerized Douglas-fir (Pseudotsugamenziesii (Mirb.) Franco), ponderosa pine (Pinusponderosa Dougl. ex Laws.), Sitka spruce (Piceasitchensis (Bong.) Carr.), and western hemlock (Tsugaheterophylla (Raf.) Sarg.) seedlings to detect ecotypic variation in colonization success and effects on seedling growth. All isolates formed well-developed ectomycorrhizae on all inoculated seedlings. Abundance ratings of short roots colonized did not differ between the four isolates for any conifer species; most inoculated seedlings developed ectomycorrhizae on over 80% of their short roots. There were also no differences between isolates in affecting seedling height, stem diameter, and dry weight of tops and roots for all conifer species. Inoculations did not improve seedling growth over uninoculated controls. Uninoculated controls of Douglas-fir and ponderosa pine had significantly greater dry weight than their inoculated seedlings; growth of Sitka spruce and western hemlock seedlings was not affected by any inoculation treatment. Laccarialaccata vigorously colonized the entire container rooting substrate and appears a prime candidate for artificial ectomycorrhizal inoculation of containerized western conifers.

Use of the ectomycorrhizal fungus Laccarialaccata in forestry. III. Effects of commercially produced inoculum on container-grown Douglas-fir and ponderosa pine seedlings

1986 ◽  
Vol 16 (4) ◽  
pp. 802-806 ◽  
Author(s):  
Ling-Ling L. Hung ◽  
Randy Molina
Keyword(s):  
Seedling Growth ◽  
Ponderosa Pine ◽  
Ectomycorrhizal Fungus ◽  
Douglas Fir ◽  
Greenhouse Experiment ◽  
Optimal Rate ◽  
Pine Seedlings ◽  
Container Nurseries

Inoculation of container-grown Douglas-fir and ponderosa pine seedlings with vegetative inoculum of Laccarialaccata (Scop.:Fr.) Berk. & Br., prepared by Sylvan Spawn Laboratory, yielded ectomycorrhizae in a research greenhouse experiment; however, in two container nurseries, ectomycorrhizae were observed only on inoculated Douglas-fir seedlings. Successfully colonized seedlings had more short roots than did the controls. The percentage of L. laccata ectomycorrhizae increased with increasing inoculation rates. Inoculation rate for best seedling growth and ectomycorrhiza formation differed between nurseries. Inoculation at an "optimal" rate resulted in quality seedlings and abundant ectomycorrhiza formation at each nursery. Inoculation of Douglas-fir with L. laccata in a commercial nursery application is feasible.

scholarly journals Pathogenicity and Virulence of Pythium Species Obtained from Forest Nursery Soils on Douglas-Fir Seedlings

Plant Disease ◽  
2013 ◽  
Vol 97 (6) ◽  
pp. 744-748 ◽  
Author(s):  
Jerry E. Weiland ◽  
Bryan R. Beck ◽  
Anne Davis
Keyword(s):  
Pseudotsuga Menziesii ◽  
Seedling Survival ◽  
The United States ◽  
Douglas Fir ◽  
Tree Seedlings ◽  
Pythium Species ◽  
Forest Nursery ◽  
Damping Off ◽  
Greenhouse Study ◽  
Forest Nurseries

Pythium species are common soilborne oomycetes that occur in forest nursery soils throughout the United States. Numerous species have been described from nursery soils. However, with the exception of P. aphanidermatum, P. irregulare, P. sylvaticum, and P. ultimum, little is known about the potential for other Pythium species found in nursery soils to cause damping-off of tree seedlings. A greenhouse study was conducted to evaluate the pathogenicity and virulence of 44 Pythium isolates representing 16 species that were originally recovered from soil at three forest nurseries in Washington and Oregon. Seeds of Douglas-fir (Pseudotsuga menziesii) were planted into soil infested with each of the isolates. Seedling survival, the number of surviving seedlings with necrotic root lesions, and taproot length were evaluated 4 weeks later. Responses of Douglas-fir to inoculation varied significantly depending on Pythium species and isolate. Eight species (P. dissotocum, P. irregulare, P. aff. macrosporum, P. mamillatum, P. aff. oopapillum, P. rostratifingens, P. sylvaticum, and P. ultimum var. ultimum) significantly reduced the number of surviving seedlings compared to the noninoculated treatment. However, all Pythium species caused a greater percentage of seedlings to develop root lesions (total mean 40%) than was observed from noninoculated seedlings (17%). Taproot length varied little among Pythium treatments and was not a useful character for evaluating pathogenicity. Results confirm the ability of P. irregulare, P. mamillatum, and P. ultimum var. ultimum to cause damping-off of Douglas-fir seedlings, and are indicative that other species such as P. dissotocum, P. aff. macrosporum, P. aff. oopapillum, P. rostratifingens, and P. sylvaticum may also be responsible for seedling loss.

Variation and genetics of ribonucleases and phosphodiesterases in conifer seeds

1977 ◽  
Vol 55 (6) ◽  
pp. 711-717 ◽  
Author(s):  
L. Mejnartowicz ◽  
F. Bergmann
Keyword(s):  
Norway Spruce ◽  
Scots Pine ◽  
Douglas Fir ◽  
Test Material ◽  
Starch Gel Electrophoresis ◽  
Conifer Species ◽  
Endosperm Tissue ◽  
Isoenzyme Variation ◽  
Conifer Seeds ◽  
Starch Gel

Using techniques of starch gel electrophoresis, isoenzymes of ribonuclease 11 (RNase, EC 3.1.4.23) and phosphodiesterase I (PDase, EC 3.1.4.1) could be identified in endosperm tissue from dry seeds of three conifer species: Norway spruce (Picea abies). Scots pine (Pinus silvestris), and Douglas fir (Pseudotsuga menziesii). The RNase patterns mostly exhibited a relatively great number of isoenzyme bands as well as a considerable tree-to-tree variation, whereas the PDase system revealed only one enzyme zone in each of the three seed species. Furthermore, an isoenzyme variation within the PDase zones appeared to be very infrequent and could only be detected in Norway spruce and Douglas fir. However, the isoenzyme patterns of RNase as well as PDase showed significant differences between the conifer species. The genetic basis of the intraspecific isoenzyme variations could be easily analyzed, since the test material (seed endosperm) represented haploid tissues resulting from macrogametophytes after fertilization. Hence, it was possible to identify three polymorphic RNase gene loci in Douglas fir seeds, two in Scots pine seeds, and one in Norway spruce seeds. The PDase zone in each conifer species was controlled by one gene locus which revealed allelic forms only in Norway spruce and Douglas fir seeds.

Isolation and characterization of nitrate reductase deficient mutants of the ectomycorrhizal fungus Hebeloma cylindrosporum

1998 ◽  
Vol 140 (2) ◽  
pp. 311-318 ◽  
Author(s):  
ROLAND MARMEISSE ◽  
PATRICIA JARGEAT ◽  
FRANCOISE WAGNER ◽  
GILLES GAY ◽  
JEAN-CLAUDE DEBAUD
Keyword(s):  
Nitrate Reductase ◽  
Ectomycorrhizal Fungus ◽  
Hebeloma Cylindrosporum ◽  
Isolation And Characterization

scholarly journals Evaluation of the provenance plot “Hrubá Skála” (Northern Bohemia) with grand fir at the age of 36 years

2017 ◽  
Vol 63 (No. 2) ◽  
pp. 75-87 ◽  
Author(s):  
Fulín Martin ◽  
Novotný Petr ◽  
Podrázský Vilém ◽  
Beran František ◽  
Dostál Jaroslav ◽  
...  
Keyword(s):  
Czech Republic ◽  
Norway Spruce ◽  
Coastal Region ◽  
Tree Height ◽  
Douglas Fir ◽  
Stem Volume ◽  
Silver Fir ◽  
The Czech Republic ◽  
The North ◽  
Oregon Cascades

The article aims to evaluate the research provenance plot established in 1980 in locality No. 214 – Hrubá Skála (in the north of the Czech Republic), where nine provenances of grand fir (Abies grandis (Douglas ex D. Don) Lindley) provided in the framework of the International Union of Forest Research Organizations project, and one provenance of grand fir, Norway spruce, silver fir and Douglas-fir from a standard commercial source are tested. We present the results of tree height, stem DBH, stem volume production and health status after 36 years. The results correspond with similar experiments in the Czech Republic and abroad and suggest that grand fir provenances from Vancouver Island (British Columbia, Canada) and the Washington (USA) State coastal region show the best production features, while the Oregon Cascades, Idaho and Montana provenances grow more slowly. Comparison with other tree species indicates that the production of grand fir at the investigated age exceeds the production of both Norway spruce and silver fir, and equalizes or gently exceeds even Douglas-fir.

scholarly journals Ectomycorrhizal Community on Norway Spruce Seedlings Following Bark Beetle Infestation

Forests ◽  
2019 ◽  
Vol 10 (9) ◽  
pp. 740 ◽  
Author(s):  
Petra Veselá ◽  
Martina Vašutová ◽  
Karolína Hofmannová ◽  
Magda Edwards-Jonášová ◽  
Pavel Cudlín
Keyword(s):  
Norway Spruce ◽  
Bark Beetle ◽  
Seedling Growth ◽  
Forest Type ◽  
Growth Parameters ◽  
Forest Disturbances ◽  
Phialocephala Fortinii ◽  
Exploration Types ◽  
Seedling Roots ◽  
Mycelial Network

Ectomycorrhizal (ECM) fungi importantly influence seedling growth, nutrition, and survival and create an extensive mycelial network interconnecting tree species and enabling resource redistribution. Due to their symbiotic relationship with trees, they are impacted by forest disturbances, which are of increasing relevance due to climate change. The effect of disturbance on seedling colonization and their morphology is still largely unknown. Seedling growth parameters and the ECM fungal assemblage on the roots of Norway spruce (Picea abies (L.) H. Karst.) seedlings were assessed in mature spruce forests attacked and destroyed by bark beetle and in a mature non-attacked forest as a reference. We did not detect significant differences in number of ECM species on seedling roots among forest types, but ECM species composition changed; Tylospora fibrillosa (Burt) Donk, Meliniomyces variabilis Hambl. & Sigler, and Phialocephala fortinii C.J.K. Wang & H.E. Wilcox were characteristic species in the forest destroyed by bark beetle, whereas Lactarius, Cortinarius, and Russula were in the mature forest. Forest type further significantly influenced the height, root length, and root collar thickness of seedlings and the proportion of exploration types of mycorrhizae.

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