Influence of some carbon sources on growth of Cochliobolus sativus

1972 ◽  
Vol 50 (3) ◽  
pp. 683-685
Author(s):  
R. V. Clark
Keyword(s):  
Radial Growth ◽  
Mycelial Growth ◽  
Growth Response ◽  
Carbon Sources ◽  
Cochliobolus Sativus ◽  
Good Growth ◽  
Liquid Media ◽  
Differential Growth ◽  
Agar Media

Four isolates of C. sativus were able to use a number of carbon sources to varying degrees with little evidence of a differential growth response by the isolates. With most carbon sources the response was different when growth on liquid media was compared with that on agar media. Dextrin and L-sorbose were exceptions as dextrin supported good growth with both types and L-sorbose poor with both. Lactose supported the best mycelial growth on liquid media and dextrin the best radial growth and sporulation on agar media when compared with sucrose.

Mycelial growth, sclerotial production and carbon utilization of three Typhula species

1999 ◽  
Vol 77 (2) ◽  
pp. 312-317
Author(s):  
C Wu ◽  
T Hsiang
Keyword(s):  
Radial Growth ◽  
Mycelial Growth ◽  
Biocontrol Agent ◽  
Carbon Sources ◽  
Carbon Utilization ◽  
Turfgrass Disease ◽  
Snow Mould ◽  
Typhula Incarnata ◽  
And Storage ◽  
Temperature Optima

The mycelial growth, sclerotial production, and carbon utilization of the snow mould biocontrol agent Typhula phacorrhiza Fries were compared with the two grey snow mould fungi, Typhula ishikariensis Imai and Typhula incarnata Lasch ex Fries. Variation was observed among the four isolates for each species, but there was greater variation among species. All three species were able to grow at the lowest temperature (0°C), but temperature optima differed with T. ishikariensis lowest and T. phacorrhiza highest. On potato dextrose agar or potato malt agar at 10°C, T. phacorrhiza had greater radial growth than T. ishikariensis but less than T. incarnata. All species could utilize microcrystalline cellulose, bacto-cellulose, and glucose as carbon sources, but radial growth of T. phacorrhiza was significantly greater than T. incarnata and T. ishikariensis on these defined carbon sources tested, except for Indulin-AT, which was inhibitory to T. incarnata and T. phacorrhiza. This greater ability to utilize these structural and storage carbohydrates, combined with mycelial growth and sclerotial production over a wider range of temperatures, may help explain how some isolates of T. phacorrhiza are able to outcompete grey snow mould in field tests.Key words: turfgrass disease, biocontrol, psychrophilic.

Hypoxylon mammatum ascospore germination and mycelial growth on bark and wood media from young branches of trembling aspen

1976 ◽  
Vol 54 (13) ◽  
pp. 1438-1442 ◽  
Author(s):  
J. R. French ◽  
P. D. Manion
Keyword(s):  
Seasonal Variation ◽  
Radial Growth ◽  
Mycelial Growth ◽  
Nutrient Source ◽  
Wood Tissue ◽  
Inhibiting Factors ◽  
Ascospore Germination ◽  
Agar Media ◽  
Growth Promoting ◽  
Hypoxylon Mammatum

Hypoxylon mammatum radial growth rates increased with increasing concentrations of propylene-oxide-sterilized bark or wood tissue from fourth-season branches as sole nutrient source in agar media. Assay of bark and wood of one- to three-season-old branches demonstrated clonal and seasonal variation in growth-promoting and spore-germination-inhibiting factors.

Differential Growth Response of A1 and A2 Mating Types of Phytophthora infestons on Rye A and V-8 Juice Agar Media Supplemented with Rhizome Powder of Cyperus rotundus

Mycobiology ◽  
2001 ◽  
Vol 29 (3) ◽  
pp. 164-169 ◽  
Author(s):  
U. P. Singh ◽  
B. K. Sarma ◽  
Ruo Nishimura ◽  
Kiroku Kobayashi ◽  
Akira Ogoshi ◽  
...  
Keyword(s):  
Growth Response ◽  
Cyperus Rotundus ◽  
Mating Types ◽  
Differential Growth ◽  
Agar Media

scholarly journals Effect Of Different Sugars On Shoot Regeneration Of Maize (Zea Mays L.)

1970 ◽  
Vol 8 (1) ◽  
pp. 119-124 ◽  
Author(s):  
DP Gauchan
Keyword(s):  
Zea Mays ◽  
Root Growth ◽  
Shoot Regeneration ◽  
Growth Response ◽  
Carbon Sources ◽  
Seedling Development ◽  
Good Growth ◽  
Low Concentration ◽  
Basal Media

Effect of various concentrations of different sugars was investigated for induction of root and shoot from maize. The seedling development was induced with the application of fructose, maltose and sucrose at different concentrations (0.25%, 0.5%, 0.75%, 1.0%, 2.5%, 5%, and 10%) of each. Dissected embryos were transferred in ½ MS basal media fortified with various concentrations of different carbon sources. In vitro regenerated maize plantlets were healthy and attained a length of 12.2 cm at 1.0 % concentration in maltose within a week. Out of three sugars low concentration (0.25%- 2.5%) of maltose and sucrose exhibited the maximum shoot and root growth. All the concentrations of maltose and sucrose showed the good growth response of shoot and root. DOI: http://dx.doi.org/10.3126/kuset.v8i1.6051 KUSET 2012; 8(1): 119-124

Growth of psychrophilic microorganisms at subzero temperatures

1968 ◽  
Vol 14 (2) ◽  
pp. 97-101 ◽  
Author(s):  
J. M. Larkin ◽  
J. L. Stokes
Keyword(s):  
Supercooled Liquid ◽  
Good Growth ◽  
Liquid Media ◽  
Psychrophilic Bacteria ◽  
Gram Negative ◽  
Subzero Temperatures ◽  
Generation Times ◽  
The Media ◽  
Agar Media ◽  
Gram Positive Cocci

In an attempt to find a suitable medium for determining the ability of psychrophilic bacteria and yeasts to grow at subzero temperatures, six antifreeze compounds were added individually and in combination to Trypticase soy broth and agar. The media were tested for their ability to support good growth of psychrophilic bacteria at 20 °C and to resist freezing at subzero temperatures. In general, media which supported good growth at 20 °C froze at −7 °C, whereas media which did not freeze at −7 °C did not support good growth at 20 °C. In supercooled liquid media, however, three species of Bacillus grew at −2 °C, one at −4.5 °C, and five at −5 to −7 °C. Generation times were approximately 4 days at −2 °C, 7 days at −4.5 °C, and 9 to 11 days at −5 to −7 °C. On supercooled growth-supporting agar media, two Gram-positive cocci, one Gram-negative rod, and four species of yeast grew at −2 °C. The latter also grew at −4.5 °C. Three species of Bacillus grew at −7 °C, and two at −10 °C.

scholarly journals Morphological and cultural studies of Septoria vignicola

2014 ◽  
Vol 19 (1) ◽  
pp. 83-89
Author(s):  
R. D. Rawal ◽  
H. S. Sohi
Keyword(s):  
Mycelial Growth ◽  
Culture Media ◽  
Agar Medium ◽  
Carbon Sources ◽  
Potassium Nitrate ◽  
Potato Dextrose Agar ◽  
Optimum Growth ◽  
Carbon Supply ◽  
Wide Range ◽  
Agar Media

Pycnidial formation starts six days after inoculation. These arę globose, dark and range between 59.28-109.20 μm in diameier. The pycnidial wall is composed of 2- 3 layers of brown cells. The pycnidiospores ooze 8 days after inoculation. They are filiform, straight to curved, hyaline, septate with 2-4 septa, ends pointed or round and 21.84-68.64 x 1.25-2.15 μm. Mycelial growth was most profuse on Czapek Dox's agar medium, whereas maximum sporulation took place on Coon's, Sabauraud's and Potato dextrose agar media. The fungus could grow over a wide range of temperature from 12- 36°C (optimum: 24 ± l°C) and showed no growth at 40°C or above. Pycnidial formation occurred at 16- 28°C with a maximum at 24 ± l°C. Out of seven carbon sources tested, sorbose was the best for growth and sporulation of <i>Septoria vignicola</i> whereas maltose was a poor source for sporulation. The fungus responded very well to the supply of carbon in culture media as no growth of the fungus could be observed when the carbon supply was with held. Aspartic acid supported optimum growth among all the organic sources tried, whereas among inorganic sources potassium nitrate was the best, last growth observed on ammonium nitrate.

SUPPRESSION AND STIMULATION OF MYCELIAL GROWTH OF PHYTOPHTHORA CRYPTOGEA BY CERTAIN THIAMINE-REQUIRING AND THIAMINE-SYNTHESIZING BACTERIA

1961 ◽  
Vol 7 (6) ◽  
pp. 945-950 ◽  
Author(s):  
D. C. Erwin ◽  
H. Katznelson
Keyword(s):  
Radial Growth ◽  
Mycelial Growth ◽  
Synthetic Medium ◽  
Growth Reduction ◽  
Phytophthora Cryptogea ◽  
Deficient Medium ◽  
Agar Media ◽  
Stimulation Of

Preculturing a synthetic medium with bacteria of the genus Arthrobacter reduced growth of Phytophthora cryptogea. The growth reduction appeared to be due chiefly to utilization of thiamine by the bacteria. However, there was some evidence that these bacteria also removed other fungal nutrients from the medium. When the bacteria were streaked on synthetic agar media, radial growth of the fungus on the same plates was reduced only when the thiamine concentration was low. Certain other bacteria stimulated mycelial growth of P. cryptogea in a thiamine-deficient medium by producing either thiamine or thiamine-like substances.

scholarly journals Preparation of uniform dispersions of cholesterol and other water-insoluble carbon sources in agar media

1962 ◽  
Vol 3 (2) ◽  
pp. 275-276
Author(s):  
G.E. Peterson ◽  
Harold L. Lewis ◽  
James R. Davis
Keyword(s):  
Carbon Sources ◽  
Agar Media

scholarly journals Radial growth response to climate change along the latitudinal range of the world's southernmost conifer in southern South America

2018 ◽  
Vol 45 (5) ◽  
pp. 1140-1152 ◽  
Author(s):  
Andrés Holz ◽  
Sarah J. Hart ◽  
Grant J. Williamson ◽  
Thomas T. Veblen ◽  
Juan C. Aravena
Keyword(s):  
Climate Change ◽  
South America ◽  
Radial Growth ◽  
Growth Response ◽  
Southern South America ◽  
Latitudinal Range

COCHLIOBOLUS SATIVUS: VI. ISOLATION OF NUTRITIONALLY EXACTING MUTANTS BY FILTRATION ENRICHMENT

1962 ◽  
Vol 40 (10) ◽  
pp. 1293-1297 ◽  
Author(s):  
R. D. Tinline
Keyword(s):  
Mass Transfer ◽  
Comparative Studies ◽  
Minimal Medium ◽  
Cochliobolus Sativus ◽  
Complete Medium ◽  
Differential Growth ◽  
Enrichment Method ◽  
Filtration Enrichment ◽  
Hyphal Tip ◽  
Methods Of Isolation

Nutritionally exacting mutants of Cochliobolus sativus, a fungus with multinucleate, multicellular spores, were readily isolated by a filtration enrichment method. The method is similar to one described by Fries and is based upon the differential growth of auxotrophs and prototrophs in minimal medium. Most of the prototrophic propagules were removed by filtration. Propagules in the filtrate were concentrated by centrifugation, plated on complete medium, and subsequently tested for nutritional requirements.In comparative studies on four methods of isolation, namely, total isolation, mass transfer; total isolation, hyphal-tip transfer; filtration enrichment, mass transfer; and filtration enrichment, hyphal-tip transfer, the yield of auxotrophic mutants was 0, 0.04, 1.16, and 1.83%, respectively.

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