A thin-layer chromatographic study of the phenolics of the genus Aegilops. I. Numerical chemotaxonomy of the diploid species

1970 ◽  
Vol 48 (10) ◽  
pp. 1775-1780 ◽  
Author(s):  
P. J. Kaltsikes ◽  
W. Dedio
Keyword(s):  
Phenolic Compounds ◽  
Thin Layer ◽  
Diploid Species ◽  
Chromatographic Study ◽  
Two Dimensional ◽  
Aegilops Species ◽  
Young Leaves ◽  
Chemotaxonomic Study ◽  
High Degree ◽  
Degree Of Similarity

A two-dimensional thin-layer chromatographic study of the phenolics of young leaves provided the means for a numerical chemotaxonomic study of the diploid species of the genus Aegilops L. A great amount of variation in types and concentration of these compounds was found within A. squarrosa. Var. anathera, typica, and meyeri, of the ssp. eusquarrosa, were more related to ssp. strangulata than to each other. However, since the subspecies are morphologically distinct, their present taxonomic position should be retained. A. comosa was distinguishable from all other species by the presence of a violet fluorescing compound, while A. mutica was distinct from the others by the presence of a blue and a yellow fluorescing compound. Members of the Sitopsis section exhibited a high degree of similarity in composition of phenolic compounds. Based on phenolic contrast, A. umbellulata should be placed in a section apart from A. caudata while the rest of the diploid species should remain as presently classified. All Aegilops species were distinctly different from T. aegilopoides and T. monococcum and should be retained as separate generic entities.

A thin-layer chromatographic study of the phenolics of the genus Aegilops. II. Numerical chemotaxonomy of the polyploid species

1970 ◽  
Vol 48 (10) ◽  
pp. 1781-1786 ◽  
Author(s):  
P. J. Kaltsikes ◽  
W. Dedio
Keyword(s):  
Thin Layer ◽  
Genetic Material ◽  
Natural Hybridization ◽  
Chromatographic Study ◽  
Aegilops Species ◽  
Polyploid Species ◽  
D Genome ◽  
Young Leaves ◽  
New Compounds ◽  
Hybridization And Introgression

Based upon a thin-layer chromatographic study of the phenolics of young leaves of the species of the genus Aegilops, in which affinities both within and among diploid and polyploid groups were compared, the following conclusions were drawn. No new compounds were found in the polyploid Aegilops species. A. ovata must have arisen from A. umbellulata and A. comosa. A. columnaris probably contains genetic material derived from members of the Sitopsis section. A. biuncialis contains parts of the genome of A. comosa and parts from some member of the Sitopsis section. A. variabilis contains an S or modified S genome. A. triuncialis contains the C and Cu genomes. A. cylindrica contains the genomes C and D. A. crassa(4x) has an S genome in its constitution. A. ventricosa seems to contain parts of the A. caudata genome. A. crassa (6x) contains one D genome, probably from A. squarrosa, one from A. bicornis, and the third genome appears to contain parts from the M and S genomes. A. juvenalis and A. triaristata (6x) contain a modified S genome.The present study supports the hypothesis that extensive natural hybridization and introgression has taken place during the evolution of the polyploid species.

scholarly journals Thin-layer chromatographic study of the phenolics of the Pleurocladula species (Hepaticae)

2014 ◽  
Vol 49 (1-2) ◽  
pp. 77-83 ◽  
Author(s):  
Maria Krzakowa
Keyword(s):  
Phenolic Compounds ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Chromatographic Study ◽  
Two Dimensional ◽  
Layer Chromatography

Phenolic compounds in <em>Pleurocladula albescens</em> (Hook.) Grolle and <em>Pleurocladuia islandica</em> (Nees) Grolle were studied by two-dimensional thin-layer chromatography. Consistent differences between both taxa were found.

The biosystematics of the genus Lotus (Leguminosae) in Canada. II. Numerical chemotaxonomy

1968 ◽  
Vol 46 (5) ◽  
pp. 585-589 ◽  
Author(s):  
William F. Grant ◽  
Ilse I. Zandstra
Keyword(s):  
Chromosome Number ◽  
Thin Layer ◽  
Native Species ◽  
Cytological Study ◽  
Diploid Species ◽  
Basic Chromosome Number ◽  
Chromatographic Study ◽  
Chemical Identification ◽  
Basic Chromosome ◽  
Fluorescent Compounds

A thin-layer chromatographic study of fluorescent compounds present in native (L. denticulatus, L. formosissimus, L. micranthus, L. pinnatus, L. purshianus) and introduced (L. corniculatus, L. krylovii, L.pedunculatus, L. tenuis) Canadian species of Lotus has been carried out and relationships of the species have been determined on the basis of the coefficients of association of these compounds. Chemical identification of the compounds was not attempted, but test reagents indicated a number to be phenolics. The analysis supported the general taxonomic relationships of the species based on a morphological and cytological study. Of the native species, L. pinnatus and L. formosissimus were the most closely related, with a coefficient of association of 83.33. Lotus denticulatus, the only native species with a chromosome number of n = 6, in general showed lower coefficients of association with the n = 7 species. Of the introduced species, all of which belong to the L. corniculatus group with a basic chromosome number of 6, L. krylovii and L. tenuis had the highest coefficient of association, 75.86. Based on their coefficients of association, both of these diploid species were more closely related to the tetraploid L. corniculatus than to the diploid L. pedunculatus.

scholarly journals Thin-layer chromatography analysis and scavenging activity of marigold (Calendula officinalis L) extracts

2003 ◽  
pp. 93-102 ◽  
Author(s):  
Gordana Cetkovic ◽  
Sonja Djilas ◽  
Jasna Canadanovic-Brunet ◽  
Vesna Tumbas
Keyword(s):  
Phenolic Compounds ◽  
Thin Layer ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Spectrophotometric Method ◽  
Scavenging Activity ◽  
Two Dimensional ◽  
Calendula Officinalis ◽  
Calendula Officinalis L ◽  
Layer Chromatography

The methanol, petroleum ether, chloroform, ethyl acetate, n-butanol and water extracts were obtained by extraction of marigold flower (Calendula officinalis L). The content of total phenolic compounds, determined by UV spectrophotometric method using the Folin-Ciocalteu reagent, was 15.12 mg/g. The content of total flavonoids, determined by UV spectrophotometric method according to Markham, was 5.13 mg/g. Qualitative determination of phenolic compounds in the extracts was performed by one- and two-dimensional thin-layer chromatography (TLC) procedures. The results of one- and two-dimensional TLC analyses showed that different flavonoids and phenolic acids were present in the investigated extracts. The greatest number of flavonoids (rutin, quercetin and some unidentified flavonoid glycosides) and phenolic acids (chlorogenic, caffeic, coumaric and vanillic acid) were deteminated in methanol extract. The influence of marigold extracts, in concentration range 0.6-1.2 mg/mL, on 2,2?-diphenyl-1-picrylhydrazyl (DPPH) free radicals was investigated by electron spin resonance (ESR) spectroscopy. All extracts showed scavenging activity (SA) in the following order: ethyl acetate > n-butanol > methanol > water > chloroform > petroleum ether. The SA increased with increasing concentration of extracts. The ethyl acetate and n-butanol extracts exibited the most significant SA. These extracts in concentration of 1.2 mg/mL eliminated completely DPPH radicals. The lowest SA had chloroform and petroleum ether extracts (in concentration of 0.6 mg/mL SA=0%). The SA of marigold extracts is attributed to its hydrogen-donating ability and scavenging effect.

Two-dimensional Thin Layer Chromatographic Separation of Phenolic Compounds from Eupatorium cannabinum Extracts and their Antioxidant Activity

2012 ◽  
Vol 8 (1) ◽  
pp. 118-131 ◽  
Author(s):  
Miroslaw A. Hawryl ◽  
Renata Nowak ◽  
Monika Waksmundzka-Hajnos ◽  
Ryszard Swieboda ◽  
Monika Robak
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Thin Layer ◽  
Chromatographic Separation ◽  
Two Dimensional ◽  
Eupatorium Cannabinum

Two-Dimensional Thin Layer Chromatographic Procedure for the Identification of Dye Intermediates in Arylamine Oxidation Hair Dyes

1966 ◽  
Vol 49 (5) ◽  
pp. 954-959 ◽  
Author(s):  
Christine M Kottemann
Keyword(s):  
Thin Layer ◽  
Acidic Solution ◽  
Chromatographic Study ◽  
Basic Solution ◽  
Two Dimensional ◽  
Hair Dyes ◽  
Initial Development ◽  
Chromatographic Procedure ◽  
Color Reactions ◽  
Spray Reagents

Abstract Dye intermediates in arylamine oxidation hair dyes are identified by a two-dimensional thin layer chromatographic method. An initial development in basic solution is followed by development in an acidic solution. The dye intermediates are identified by their color reactions with spray reagents and by their spatial relationships to known intermediates developed on the same plate. The chromatographic study included 30 dye intermediates proposed for use in commercial oxidation-type hair dyes.

A thin-layer chromatographic study of the phenolics of Triticale and its parental species

1969 ◽  
Vol 47 (10) ◽  
pp. 1589-1593 ◽  
Author(s):  
W. Dedio ◽  
P. J. Kaltsikes ◽  
E. N. Larter
Keyword(s):  
Thin Layer ◽  
Chinese Spring ◽  
Wheat Variety ◽  
Similarity Measures ◽  
Correlation Coefficients ◽  
Chromatographic Study ◽  
Wheat Varieties ◽  
Close Relationship ◽  
Young Leaves ◽  
Wheat Parent

The phenolics of young leaves from a hexaploid and three octaploid Triticale lines, and their rye and wheat parents were investigated by two dimensional thin-layer chromatography. Matching coefficients, coefficients of similarity, biochemical distances, and correlation coefficients were calculated to determine the degree of similarity of phenolics between the different groups. Chromatographic patterns and the calculated similarity measures showed a close relationship of phenolics between Triticale and its wheat parent. These patterns were even more closely related if the wheat parent was hexaploid. Two wheat varieties (Prelude and Kharkov) had identical chromatographic patterns while the variety Chinese Spring showed a reduced number of spots. The pattern of Stewart, a durum wheat variety, was intermediate between Chinese Spring and Prelude or Kharkov. Generally, the number of flavonoid compounds increased with the level of ploidy but did not increase proportionally with the number of genomes.

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