scholarly journals Thin-layer chromatography analysis and scavenging activity of marigold (Calendula officinalis L) extracts

2003 ◽  
pp. 93-102 ◽  
Author(s):  
Gordana Cetkovic ◽  
Sonja Djilas ◽  
Jasna Canadanovic-Brunet ◽  
Vesna Tumbas
Keyword(s):  
Phenolic Compounds ◽  
Thin Layer ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Spectrophotometric Method ◽  
Scavenging Activity ◽  
Two Dimensional ◽  
Calendula Officinalis ◽  
Calendula Officinalis L ◽  
Layer Chromatography

The methanol, petroleum ether, chloroform, ethyl acetate, n-butanol and water extracts were obtained by extraction of marigold flower (Calendula officinalis L). The content of total phenolic compounds, determined by UV spectrophotometric method using the Folin-Ciocalteu reagent, was 15.12 mg/g. The content of total flavonoids, determined by UV spectrophotometric method according to Markham, was 5.13 mg/g. Qualitative determination of phenolic compounds in the extracts was performed by one- and two-dimensional thin-layer chromatography (TLC) procedures. The results of one- and two-dimensional TLC analyses showed that different flavonoids and phenolic acids were present in the investigated extracts. The greatest number of flavonoids (rutin, quercetin and some unidentified flavonoid glycosides) and phenolic acids (chlorogenic, caffeic, coumaric and vanillic acid) were deteminated in methanol extract. The influence of marigold extracts, in concentration range 0.6-1.2 mg/mL, on 2,2?-diphenyl-1-picrylhydrazyl (DPPH) free radicals was investigated by electron spin resonance (ESR) spectroscopy. All extracts showed scavenging activity (SA) in the following order: ethyl acetate > n-butanol > methanol > water > chloroform > petroleum ether. The SA increased with increasing concentration of extracts. The ethyl acetate and n-butanol extracts exibited the most significant SA. These extracts in concentration of 1.2 mg/mL eliminated completely DPPH radicals. The lowest SA had chloroform and petroleum ether extracts (in concentration of 0.6 mg/mL SA=0%). The SA of marigold extracts is attributed to its hydrogen-donating ability and scavenging effect.

scholarly journals PHARMACOGNOSTIC STANDARDIZATION, PRELIMINARY PHYTOCHEMICAL SCREENING AND TLC FINGERPRINTING OF THE BARK OF CASCABELA THEVETIA L.

2019 ◽  
pp. 125-130
Author(s):  
Neelutpal Gogoi ◽  
Biman Bhuyan ◽  
Trinayan Deka
Keyword(s):  
Thin Layer ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Thin Layer Chromatography ◽  
Water Soluble ◽  
Phytochemical Analysis ◽  
Alcoholic Extract ◽  
Phytochemical Screening ◽  
Cork Cell ◽  
Layer Chromatography

Objectives: In this study, systematic pharmacognostic study and preliminary phytochemical screening of the bark of Cascabela thevetia L. were carried out. Methods: The selected plant part was collected, processed and stored in an airtight container. From the bark different pharmacognostic studies like macroscopic and microscopic evaluation, physicochemical parameters, fluorescence analysis were done. Powdered bark was successively extracted by petroleum ether, chloroform, ethyl acetate, and methanol using a Soxhlet apparatus and finally macerated with the hydro-alcoholic solvent system (30:70). The preliminary phytochemical analysis and thin layer chromatography of the extracts were done to find the nature and number of the different phytoconstituents present. Results: Transverse microscopy reveals the presence of crystal oxalate, cork cell, starch granules, vascular bundle, phloem fiber, parenchyma cells, and collenchyma cells. Powder microscopy also showed the presence of cork cell, fiber and calcium oxalate crystal. Results obtained in different physicochemical analysis like total ash, acid insoluble ash, water soluble ash, alcohol-soluble extractive, water-soluble extractive, and moisture content were 8.67%, 0.83%, 5.33%, 4.53%, 12.27%, and 7.83% respectively. Phytochemical analysis showed the presence of alkaloid, flavonoid, triterpenoid, phytosterol, tannin, saponin, anthraquinone, carbohydrate and fatty acid in the different extracts. TLC (Thin Layer Chromatography) study revealed 4 spots in petroleum ether, chloroform, ethyl acetate, and methanol extracts and 3 spots in the Hydro-alcoholic extract with different solvent systems. Conclusion: The results obtained from the study will provide a reliable basis for identification, purity, and quality of the plant.

scholarly journals QUANTITATIVE PHYTOCHEMICAL SCREENING, THIN-LAYER CHROMATOGRAPHY ANALYSIS, HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY FINGERPRINTING, AND ANTIOXIDANT ACTIVITY OF LEAVES OF DIOSPYROS MONTANA (ROXB.)

2019 ◽  
pp. 325-331
Author(s):  
ABHIJEET V PURI
Keyword(s):  
Antioxidant Activity ◽  
Thin Layer ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Butylated Hydroxytoluene ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Layer Chromatography

Objective: The objective of this study was to investigate important phytochemical constituents and antioxidant potential of Diospyros montana Roxb. leaves belonging to the family Ebenaceae. Methods: Leaves were exhaustively extracted with ethanol and fractionated into petroleum ether, chloroform, and ethyl acetate extracts. The various fractions were further analyzed for phytochemical composition and concentration-dependent antioxidant activity using conventional methods and high-performance thin-layer chromatography (HPTLC) fingerprinting. Since leaves contained phenolic compounds, extracts were evaluated for total phenolic content, flavonoids contents, and in-vitro antioxidant activity. Antioxidant potential was assessed using parameters such as superoxide radical scavenging, nitric oxide inhibition, and β-carotene/linoleic acid antioxidant activity. Results: Primitive phytochemical investigation highlighted the presence of steroids, saponins, flavonoids, alkaloids, and tannins which were confirmed by TLC and HPTLC fingerprinting. The antioxidant activity of leaf extracts decreased in the following order ethyl acetate > ethanolic > chloroform > petroleum ether and it was comparable with standards such as ascorbic acid and butylated hydroxytoluene. Conclusion: The present study concludes that the ethanolic extract and fractions of D. montana (Roxb.) leaves have prominent antioxidant activity comparable to standards. Therefore, D. montana (Roxb.) leaves may be used as a probable source of natural antioxidants in the pharmaceutical industry.

Differentiation of Geographic Origin of Spices. II. Oregano by Gas Chromatography and Thin Layer Chromatography

1969 ◽  
Vol 52 (6) ◽  
pp. 1184-1189
Author(s):  
William H Stahl Stahl ◽  
Jean N Skarzynski ◽  
William A Voelker
Keyword(s):  
Gas Chromatography ◽  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Geographic Origin ◽  
Chromatographic Technique ◽  
Two Dimensional ◽  
Second Development ◽  
Chromatographic Procedure ◽  
Layer Chromatography

Abstract Both a gas chromatographic and a thin layer chromatographic procedure have been devised to differentiate the origin of oreganos. The gas chromatographic procedure utilizes the observation that the ratio of the concentration of carvacrol to thymol in Greek oregano is approximately 7:1, whereas in Mexican oregano it is 1:1. The decision can be reinforced by a fluorescence thin layer chromatographic technique, using a hexane-ethyl acetate-tetrahydrofuran mixture as the developing solvent. A two-dimensional TLC, using acetone-ethermethylene chloride for the second development, affords a clean-cut separation of all spots and is particularly useful in the diagnosis of mixtures.

Two-Dimensional Thin Layer Chromatography of Aflatoxins in Figs

1974 ◽  
Vol 57 (6) ◽  
pp. 1398-1401
Author(s):  
Lewis Allen
Keyword(s):  
Thin Layer ◽  
Formic Acid ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Two Dimensional ◽  
Aoac Official ◽  
Layer Chromatography

Abstract A method is described for the identification and analysis of aflatoxins in figs. This method is based on 2-dimensional thin layer chromatography, using an antidiagonal spotting technique after sample cleanup in accordance with AOAC official first action Method I for nuts and nut products, 26.015-26.020. Plates were developed in the first direction with toluene-ethyl acetate-90% formic acid (5 + 4 + 1) and in the second direction with acetone-chloroform (1 + 9). Results obtained by this method and those obtained by conventional thin layer methods are comparable. The advantage of the described method, however, is that a fluorescent artifact present in figs no longer interferes in the B2-G1 region.

scholarly journals Thin-layer chromatographic study of the phenolics of the Pleurocladula species (Hepaticae)

2014 ◽  
Vol 49 (1-2) ◽  
pp. 77-83 ◽  
Author(s):  
Maria Krzakowa
Keyword(s):  
Phenolic Compounds ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Chromatographic Study ◽  
Two Dimensional ◽  
Layer Chromatography

Phenolic compounds in <em>Pleurocladula albescens</em> (Hook.) Grolle and <em>Pleurocladuia islandica</em> (Nees) Grolle were studied by two-dimensional thin-layer chromatography. Consistent differences between both taxa were found.

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