Involvement of Oct4‐type transcription factor Pou5f3 in posterior spinal cord formation in zebrafish embryos

2021 ◽  
Author(s):  
Tatsuya Yuikawa ◽  
Masaaki Ikeda ◽  
Sachiko Tsuda ◽  
Shinji Saito ◽  
Kyo Yamasu
Keyword(s):  
Spinal Cord ◽  
Transcription Factor ◽  
Zebrafish Embryos

scholarly journals Notch-regulated oligodendrocyte specification from radial glia in the spinal cord of zebrafish embryos

2008 ◽  
Vol 237 (8) ◽  
pp. 2081-2089 ◽  
Author(s):  
Ho Kim ◽  
Jimann Shin ◽  
Suhyun Kim ◽  
Justin Poling ◽  
Hae-Chul Park ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Radial Glia ◽  
Zebrafish Embryos

Visualization in zebrafish larvae of Na+ uptake in mitochondria-rich cells whose differentiation is dependent on foxi3a

2007 ◽  
Vol 292 (1) ◽  
pp. R470-R480 ◽  
Author(s):  
Masahiro Esaki ◽  
Kazuyuki Hoshijima ◽  
Sayako Kobayashi ◽  
Hidekazu Fukuda ◽  
Koichi Kawakami ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Cell Differentiation ◽  
Direct Evidence ◽  
Zebrafish Embryos ◽  
Morpholino Oligonucleotide ◽  
Gastrula Stage ◽  
Zebrafish Larvae ◽  
Transport Inhibitors ◽  
Antisense Morpholino ◽  
Na Uptake

Uptake of Na+ from the environment is an indispensable strategy for the survival of freshwater fish, as they easily lose Na+ from the plasma to a diluted environment. Nevertheless, the location of and molecules involved in Na+ uptake remain poorly understood. In this study, we utilized Sodium Green, a Na+-dependent fluorescent reagent, to provide direct evidence that Na+ absorption takes place in a subset of the mitochondria-rich (MR) cells on the yolk sac surface of zebrafish larvae. Combined with immunohistochemistry, we revealed that the Na+-absorbing MR cells were exceptionally rich in vacuolar-type H+-ATPase (H+-ATPase) but moderately rich in Na+-K+-ATPase. We also addressed the function of foxi3a, a transcription factor that is specifically expressed in the H+-ATPase-rich MR cells. When foxi3a was depleted from zebrafish embryos by antisense morpholino oligonucleotide injection, differentiation of the MR cells was completely blocked and Na+ influx was severely reduced, indicating that MR cells are the primary sites for Na+ absorption. Additionally, foxi3a expression is initiated at the gastrula stage in the presumptive ectoderm; thus, we propose that foxi3a is a key gene in the control of MR cell differentiation. We also utilized a set of ion transport inhibitors to assess the molecules involved in the process and discuss the observations.

scholarly journals Alpinia oxyphylla Fruit Extract Ameliorates Experimental Autoimmune Encephalomyelitis through the Regulation of Th1/Th17 Cells

2019 ◽  
Vol 2019 ◽  
pp. 1-15 ◽  
Author(s):  
Kuo-Kuei Huang ◽  
Meng-Nan Lin ◽  
Yi-Ling Hsu ◽  
I-Huang Lu ◽  
I-Hong Pan ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Transcription Factor ◽  
Experimental Autoimmune Encephalomyelitis ◽  
Quantitative Polymerase Chain Reaction ◽  
Histopathological Analysis ◽  
Autoimmune Encephalomyelitis ◽  
Th17 Response ◽  
Alpinia Oxyphylla ◽  
Experimental Autoimmune

Alpinia oxyphylla is a traditional Chinese medicine widely used for treating diarrhea, ulceration, and enuresis. Moreover, A. oxyphylla is effective for cognitive function improvement and nerve regeneration. Multiple sclerosis (MS) is a chronic neuronal inflammatory autoimmune disease that commonly affects young adults in high-latitude regions. The aim of this study was to evaluate the beneficial effects of A. oxyphylla in an experimental autoimmune encephalomyelitis (EAE) mouse model, which is an extensively used model for human MS. The ethanolic extract of A. oxyphylla fruit (AO-1) was orally administered to EAE mice. Our results showed AO-1 significantly reduced EAE symptoms. Histopathological analysis showed AO-1 reduced demyelination, inflammation, gliosis, and axonal swelling in the spinal cord. Furthermore, immunohistochemistry and quantitative polymerase chain reaction (qPCR) studies revealed that the infiltration of CD4+, CD8+ T cells, and CD11b+ monocytes into the spinal cord decreased in the AO-1-treated group. Mechanistically, the Th1 transcription factor T-bet, Th17 transcription factor retinoic acid receptor–related orphan receptor γ (RORγt), and inflammatory cytokines interferon (IFN)-γ and interleukin (IL)-17 were reduced in the spinal cords of mice treated with AO-1. The expression levels of T-bet and RORγt were also lowered in the spleens of those mice. Further in vitro study showed AO-1 inhibited production of IFN-γ, IL-2, and tumor necrosis factor-α from MOG35-55-peptide-stimulated splenocytes. One component isolated from AO-1, yakuchinone A, inhibited IL-17 production in vitro and reduced EAE symptoms in the mice. Collectively, our results indicate that AO-1 ameliorated the severity of EAE in mice and may involve the regulation of Th1/Th17 response. A. oxyphylla warrants further investigation, particularly regarding its clinical benefits for MS.

P118. Multiple and stage-specific functions of a Class V POU transcription factor Pou2/Pou5f1 in zebrafish embryos revealed by the transgenic approach

2010 ◽  
Vol 80 ◽  
pp. S56-S57
Author(s):  
A. Nakamoto ◽  
S. Okamoto ◽  
M. Tai ◽  
S. Saito ◽  
D. Isobe ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Zebrafish Embryos ◽  
Class V

Subtypes of hypoxia-responsive cells differentiate into neurons in spinal cord of zebrafish embryos after hypoxic stress

2016 ◽  
Vol 108 (12) ◽  
pp. 357-377 ◽  
Author(s):  
Chih-Wei Zeng ◽  
Yasuhiro Kamei ◽  
Chih-Tien Wang ◽  
Huai-Jen Tsai
Keyword(s):  
Spinal Cord ◽  
Zebrafish Embryos ◽  
Hypoxic Stress

scholarly journals Unc-4 acts to promote neuronal identity and development of the take-off circuit in the Drosophila CNS

2019 ◽  
Author(s):  
Haluk Lacin ◽  
W. Ryan Williamson ◽  
Gwyneth M. Card ◽  
James B. Skeath ◽  
James W. Truman
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Transcription Factor ◽  
Nerve Cord ◽  
Ventral Nerve Cord ◽  
Functional Organization ◽  
Neuronal Circuits ◽  
Sense Organs ◽  
Neuronal Identity ◽  
Cholinergic Neurotransmitter

ABSTRACTThe Drosophila ventral nerve cord (VNC), the fly equivalent of the spinal cord, is composed of thousands of neurons that are born from a set of individually identifiable stem cells. The VNC harbors neuronal circuits required for the execution of vital behaviors, such as flying and walking. Taking advantage of the lineage-based functional organization of the VNC and genetic tools we developed, we investigated the molecular and developmental basis of behavior by focusing on lineage-specific functions of the homeodomain transcription factor, Unc-4. We found that Unc-4 functions in lineage 11A to promote cholinergic neurotransmitter identity and suppress the GABA fate. In 7B lineage, Unc-4 promotes proper neuronal projections to the leg neuropil, the hub for leg-related neuronal circuits and a specific flight-related take-off behavior. We also uncovered that Unc-4 acts peripherally to promote the development of proprioceptive sense organs and the abilities of flies to execute specific leg-related behaviors such as walking, climbing, and grooming. Our findings, thus, initiates the work on revealing molecular and developmental events that shape the VNC related behaviors.

scholarly journals Hemato-vascular specification requires arnt1 and arnt2 genes in zebrafish embryos

2022 ◽  
Author(s):  
Hailey E Edwards ◽  
Jaclyn Paige Souder ◽  
Daniel A Gorelick
Keyword(s):  
Endothelial Cells ◽  
Transcription Factor ◽  
Transcription Factors ◽  
Progenitor Cells ◽  
Bhlh Transcription Factor ◽  
Zebrafish Embryos ◽  
Pas Domain ◽  
Hematopoietic Stem ◽  
Vascular Progenitor Cells ◽  
Bhlh Transcription Factors

During embryonic development, a subset of cells in the mesoderm germ layer are specified as hemato-vascular progenitor cells, which then differentiate into endothelial cells and hematopoietic stem and progenitor cells. In zebrafish, the transcription factor npas4l, also known as cloche, is required for the specification of hemato-vascular progenitor cells. However, it is unclear if npas4l is the sole factor at the top of the hemato-vascular specification cascade. Here we show that arnt1 and arnt2 genes are required for hemato-vascular specification. We found that arnt1;arnt2 double homozygous mutant zebrafish embryos (herein called arnt1/2 mutants), but not arnt1 or arnt2 single mutants, lack blood cells and most vascular endothelial cells. arnt1/2 mutants have reduced or absent expression of etv2 and tal1, the earliest known endothelial and hematopoietic transcription factor genes. npas4l and arnt genes are PAS domain-containing bHLH transcription factors that function as dimers. We found that Npas4l binds both Arnt1 and Arnt2 proteins in vitro, consistent with the idea that PAS domain-containing bHLH transcription factors act in a multimeric complex to regulate gene expression. Our results demonstrate that npas4l, arnt1 and arnt2 act together as master regulators of endothelial and hematopoietic cell fate. Our results also demonstrate that arnt1 and arnt2 act redundantly in a transcriptional complex containing npas4l, but do not act redundantly when interacting with another PAS domain-containing bHLH transcription factor, the aryl hydrocarbon receptor. Altogether, our data enhance our understanding of hemato-vascular specification and the function of PAS domain-containing bHLH transcription factors.

Sign in / Sign up

Export Citation Format

Share Document