P118. Multiple and stage-specific functions of a Class V POU transcription factor Pou2/Pou5f1 in zebrafish embryos revealed by the transgenic approach

2010 ◽  
Vol 80 ◽  
pp. S56-S57
Author(s):  
A. Nakamoto ◽  
S. Okamoto ◽  
M. Tai ◽  
S. Saito ◽  
D. Isobe ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Zebrafish Embryos ◽  
Class V

Involvement of Oct4‐type transcription factor Pou5f3 in posterior spinal cord formation in zebrafish embryos

2021 ◽  
Author(s):  
Tatsuya Yuikawa ◽  
Masaaki Ikeda ◽  
Sachiko Tsuda ◽  
Shinji Saito ◽  
Kyo Yamasu
Keyword(s):  
Spinal Cord ◽  
Transcription Factor ◽  
Zebrafish Embryos

Visualization in zebrafish larvae of Na+ uptake in mitochondria-rich cells whose differentiation is dependent on foxi3a

2007 ◽  
Vol 292 (1) ◽  
pp. R470-R480 ◽  
Author(s):  
Masahiro Esaki ◽  
Kazuyuki Hoshijima ◽  
Sayako Kobayashi ◽  
Hidekazu Fukuda ◽  
Koichi Kawakami ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Cell Differentiation ◽  
Direct Evidence ◽  
Zebrafish Embryos ◽  
Morpholino Oligonucleotide ◽  
Gastrula Stage ◽  
Zebrafish Larvae ◽  
Transport Inhibitors ◽  
Antisense Morpholino ◽  
Na Uptake

Uptake of Na+ from the environment is an indispensable strategy for the survival of freshwater fish, as they easily lose Na+ from the plasma to a diluted environment. Nevertheless, the location of and molecules involved in Na+ uptake remain poorly understood. In this study, we utilized Sodium Green, a Na+-dependent fluorescent reagent, to provide direct evidence that Na+ absorption takes place in a subset of the mitochondria-rich (MR) cells on the yolk sac surface of zebrafish larvae. Combined with immunohistochemistry, we revealed that the Na+-absorbing MR cells were exceptionally rich in vacuolar-type H+-ATPase (H+-ATPase) but moderately rich in Na+-K+-ATPase. We also addressed the function of foxi3a, a transcription factor that is specifically expressed in the H+-ATPase-rich MR cells. When foxi3a was depleted from zebrafish embryos by antisense morpholino oligonucleotide injection, differentiation of the MR cells was completely blocked and Na+ influx was severely reduced, indicating that MR cells are the primary sites for Na+ absorption. Additionally, foxi3a expression is initiated at the gastrula stage in the presumptive ectoderm; thus, we propose that foxi3a is a key gene in the control of MR cell differentiation. We also utilized a set of ion transport inhibitors to assess the molecules involved in the process and discuss the observations.

scholarly journals Mesendoderm specification depends on the function of Pou2, the class V POU-type transcription factor, during zebrafish embryogenesis

2012 ◽  
Vol 54 (7) ◽  
pp. 686-701 ◽  
Author(s):  
Alam Khan ◽  
Andrew Nakamoto ◽  
Miyako Tai ◽  
Shinji Saito ◽  
Yukiko Nakayama ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Class V ◽  
Zebrafish Embryogenesis

scholarly journals Hemato-vascular specification requires arnt1 and arnt2 genes in zebrafish embryos

2022 ◽  
Author(s):  
Hailey E Edwards ◽  
Jaclyn Paige Souder ◽  
Daniel A Gorelick
Keyword(s):  
Endothelial Cells ◽  
Transcription Factor ◽  
Transcription Factors ◽  
Progenitor Cells ◽  
Bhlh Transcription Factor ◽  
Zebrafish Embryos ◽  
Pas Domain ◽  
Hematopoietic Stem ◽  
Vascular Progenitor Cells ◽  
Bhlh Transcription Factors

During embryonic development, a subset of cells in the mesoderm germ layer are specified as hemato-vascular progenitor cells, which then differentiate into endothelial cells and hematopoietic stem and progenitor cells. In zebrafish, the transcription factor npas4l, also known as cloche, is required for the specification of hemato-vascular progenitor cells. However, it is unclear if npas4l is the sole factor at the top of the hemato-vascular specification cascade. Here we show that arnt1 and arnt2 genes are required for hemato-vascular specification. We found that arnt1;arnt2 double homozygous mutant zebrafish embryos (herein called arnt1/2 mutants), but not arnt1 or arnt2 single mutants, lack blood cells and most vascular endothelial cells. arnt1/2 mutants have reduced or absent expression of etv2 and tal1, the earliest known endothelial and hematopoietic transcription factor genes. npas4l and arnt genes are PAS domain-containing bHLH transcription factors that function as dimers. We found that Npas4l binds both Arnt1 and Arnt2 proteins in vitro, consistent with the idea that PAS domain-containing bHLH transcription factors act in a multimeric complex to regulate gene expression. Our results demonstrate that npas4l, arnt1 and arnt2 act together as master regulators of endothelial and hematopoietic cell fate. Our results also demonstrate that arnt1 and arnt2 act redundantly in a transcriptional complex containing npas4l, but do not act redundantly when interacting with another PAS domain-containing bHLH transcription factor, the aryl hydrocarbon receptor. Altogether, our data enhance our understanding of hemato-vascular specification and the function of PAS domain-containing bHLH transcription factors.

scholarly journals Grhl3 promotes retention of epidermal cells under endocytic stress to maintain epidermal architecture in zebrafish

PLoS Genetics ◽  
2021 ◽  
Vol 17 (9) ◽  
pp. e1009823
Author(s):  
Mandar Phatak ◽  
Shruti Kulkarni ◽  
Lee B. Miles ◽  
Nazma Anjum ◽  
Sebastian Dworkin ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Transcription Factor ◽  
Cell Adhesion ◽  
Transcriptome Analysis ◽  
Genetic Interaction ◽  
Epidermal Cells ◽  
Tissue Homeostasis ◽  
Zebrafish Embryos ◽  
Epithelial Homeostasis ◽  
Cell Extrusion

Epithelia such as epidermis cover large surfaces and are crucial for survival. Maintenance of tissue homeostasis by balancing cell proliferation, cell size, and cell extrusion ensures epidermal integrity. Although the mechanisms of cell extrusion are better understood, how epithelial cells that round up under developmental or perturbed genetic conditions are reintegrated in the epithelium to maintain homeostasis remains unclear. Here, we performed live imaging in zebrafish embryos to show that epidermal cells that round up due to membrane homeostasis defects in the absence of goosepimples/myosinVb (myoVb) function, are reintegrated into the epithelium. Transcriptome analysis and genetic interaction studies suggest that the transcription factor Grainyhead-like 3 (Grhl3) induces the retention of rounded cells by regulating E-cadherin levels. Moreover, Grhl3 facilitates the survival of MyoVb deficient embryos by regulating cell adhesion, cell retention, and epidermal architecture. Our analyses have unraveled a mechanism of retention of rounded cells and its importance in epithelial homeostasis.

Hey2 acts upstream of Notch in hematopoietic stem cell specification in zebrafish embryos

Blood ◽  
2010 ◽  
Vol 116 (12) ◽  
pp. 2046-2056 ◽  
Author(s):  
Jonathan M. Rowlinson ◽  
Martin Gering
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Transcription Factor ◽  
Stem Cell ◽  
Growth Factor ◽  
Notch Signaling ◽  
Dorsal Aorta ◽  
Zebrafish Embryos ◽  
Vascular Endothelial ◽  
Hematopoietic Stem ◽  
Endothelial Growth Factor

Abstract Hematopoietic stem cells (HSCs) are essential for homeostasis and injury-induced regeneration of the vertebrate blood system. Although HSC transplantations constitute the most common type of stem cell therapy applied in the clinic, we know relatively little about the molecular programming of HSCs during vertebrate embryogenesis. In vertebrate embryos, HSCs form in close association with the ventral wall of the dorsal aorta. We have shown previously that in zebrafish, HSC formation depends on the presence of a signaling cascade that involves Hedgehog, vascular endothelial growth factor, and Notch signaling. Here, we reveal that Hey2, a hairy/enhancer-of-split–related basic helix-loop-helix transcription factor often believed to act downstream of Notch, is also required for HSC formation. In dorsal aorta progenitors, Hey2 expression is induced downstream of cloche and the transcription factor Scl/Tal1, and is maintained by Hedgehog and vascular endothelial growth factor signaling. Whereas knockdown of Hey2 expression results in a loss of Notch receptor expression in dorsal aorta angioblasts, activation of Notch signaling in hey2 morphants rescues HSC formation in zebrafish embryos. These results establish an essential role for Hey2 upstream of Notch in HSC formation.

The transcription factor, glial cell missing 2, is involved in differentiation and functional regulation of H+-ATPase-rich cells in zebrafish (Danio rerio)

2009 ◽  
Vol 296 (4) ◽  
pp. R1192-R1201 ◽  
Author(s):  
Wei-Jen Chang ◽  
Jiun-Lin Horng ◽  
Jia-Jiun Yan ◽  
Chung-Der Hsiao ◽  
Pung-Pung Hwang
Keyword(s):  
Transcription Factor ◽  
In Situ Hybridization ◽  
Glial Cell ◽  
Cell Fate ◽  
Zebrafish Embryos ◽  
Complete Disappearance ◽  
Apical Surface ◽  
Atpase Subunit ◽  
Functional Regulation

H+-ATPase-rich (HR) cells in zebrafish are known to be involved in acid secretion and Na+ uptake mechanisms in zebrafish gills/skin; however, little is known about how HR cells are functionally regulated. In the present work, we studied the roles of Drosophila glial cell missing ( gcm), a cell fate-related transcription factor, in the differentiation and functional regulation of zebrafish HR cells. Zebrafish gcm2 ( zgcm2) was found to begin expression in zebrafish embryos at 10 h postfertilization (hpf), and to be extensively expressed in gills but only mildly so in eyes, heart, muscles, and testes. By whole mount in situ hybridization, zgcm2 mRNA signals were found in a group of cells on the zebrafish yolk sac surface initially in the tail bud stage (10 hpf); they had disappeared at 36 hpf and thereafter appeared again in the gill region from 48 hpf. Double fluorescence in situ hybridization further demonstrated specific colocalization of zgcm2 mRNA in HR cells in zebrafish embryos. Knockdown of zgcm2 with a specific morpholino oligonucleotide caused the complete disappearance of HR cells with a concomitant decrease in H+ activity at the apical surface of HR cells, but it did not affect the occurrence of Na+-K+-ATPase-rich cells. A decrease in the H+-ATPase subunit A ( zatp6v1a) expression and no change in zgcm2 expression in zebrafish gills were seen from 12 h to 3 days after transfer to acidic fresh water, but a compensatory stimulation in the expressions of both genes appeared 4 days posttransfer. In conclusion, functional regulation of HR cells is probably achieved by enhancing cell differentiation via zGCM2 activation.

scholarly journals Insights into early vasculogenesis revealed by expression of the ETS-domain transcription factor Fli-1 in wild-type and mutant zebrafish embryos

2000 ◽  
Vol 90 (2) ◽  
pp. 237-252 ◽  
Author(s):  
Louise A Brown ◽  
Adam R.F Rodaway ◽  
Thomas F Schilling ◽  
Trevor Jowett ◽  
Philip W Ingham ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Zebrafish Embryos ◽  
Wild Type ◽  
Ets Domain
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