scholarly journals Notch-regulated oligodendrocyte specification from radial glia in the spinal cord of zebrafish embryos

2008 ◽  
Vol 237 (8) ◽  
pp. 2081-2089 ◽  
Author(s):  
Ho Kim ◽  
Jimann Shin ◽  
Suhyun Kim ◽  
Justin Poling ◽  
Hae-Chul Park ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Radial Glia ◽  
Zebrafish Embryos

An Early Developmental Marker for Radial Glia in Rat Spinal Cord

1996 ◽  
Vol 54 ◽  
pp. 36-37
Author(s):  
V. Kriho ◽  
H.-Y. Yang ◽  
C.-M. Lue ◽  
N. Lieska ◽  
G. D. Pappas
Keyword(s):  
Spinal Cord ◽  
Intermediate Filament ◽  
Radial Glia ◽  
Rat Spinal Cord ◽  
Future Studies ◽  
Spinal Cord Development ◽  
Median Septum ◽  
Differentiation Marker ◽  
Early Developmental ◽  
Developing Rat

Radial glia have been classically defined as those early glial cells that radially span their thin processes from the ventricular to the pial surfaces in the developing central nervous system. These radial glia constitute a transient cell population, disappearing, for the most part, by the end of the period of neuronal migration. Traditionally, it has been difficult to definitively identify these cells because the principal criteria available were morphologic only.Using immunofluorescence microscopy, we have previously defined a phenotype for radial glia in rat spinal cord based upon the sequential expression of vimentin, glial fibrillary acidic protein and an intermediate filament-associated protein, IFAP-70/280kD. We report here the application of another intermediate filament-associated protein, IFAP-300kD, originally identified in BHK-21 cells, to the immunofluorescence study of radial glia in the developing rat spinal cord.Results showed that IFAP-300kD appeared very early in rat spinal cord development. In fact by embryonic day 13, IFAP-300kD immunoreactivity was already at its peak and was observed in most of the radial glia which span the spinal cord from the ventricular to the subpial surfaces (Fig. 1). Interestingly, from this time, IFAP-300kD immunoreactivity diminished rapidly in a dorsal to ventral manner, so that by embryonic day 16 it was detectable only in the maturing macroglial cells in the marginal zone of the spinal cord and the dorsal median septum (Fig. 2). By birth, the spinal cord was essentially immuno-negative for this IFAP. Thus, IFAP-300kD appears to be another differentiation marker available for future studies of gliogenesis, especially for the early stages of radial glia differentiation.

scholarly journals Glutamate Transporter GLAST Is Expressed in the Radial Glia–Astrocyte Lineage of Developing Mouse Spinal Cord

1997 ◽  
Vol 17 (23) ◽  
pp. 9212-9219 ◽  
Author(s):  
Takashi Shibata ◽  
Keiko Yamada ◽  
Masahiko Watanabe ◽  
Kazuhiro Ikenaka ◽  
Keiji Wada ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Radial Glia ◽  
Glutamate Transporter ◽  
Mouse Spinal Cord

Embryonic radial glia bridge spinal cord lesions and promote functional recovery following spinal cord injury

2005 ◽  
Vol 193 (2) ◽  
pp. 394-410 ◽  
Author(s):  
Koichi Hasegawa ◽  
Yu-Wen Chang ◽  
Hedong Li ◽  
Yana Berlin ◽  
Osamu Ikeda ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Functional Recovery ◽  
Radial Glia ◽  
Spinal Cord Lesions ◽  
Cord Injury

Morphology and differentiation of radial glia in the developing rat spinal cord

2002 ◽  
Vol 454 (3) ◽  
pp. 263-271 ◽  
Author(s):  
Siobhan S. McMahon ◽  
Kieran W. McDermott
Keyword(s):  
Spinal Cord ◽  
Radial Glia ◽  
Rat Spinal Cord ◽  
Developing Rat

scholarly journals Radial glia inhibit peripheral glial infiltration into the spinal cord at motor exit point transition zones

Glia ◽  
2016 ◽  
Vol 64 (7) ◽  
pp. 1138-1153 ◽  
Author(s):  
Cody J. Smith ◽  
Kimberly Johnson ◽  
Taylor G. Welsh ◽  
Michael J. F. Barresi ◽  
Sarah Kucenas
Keyword(s):  
Spinal Cord ◽  
Radial Glia ◽  
Exit Point ◽  
Transition Zones

Involvement of Oct4‐type transcription factor Pou5f3 in posterior spinal cord formation in zebrafish embryos

2021 ◽  
Author(s):  
Tatsuya Yuikawa ◽  
Masaaki Ikeda ◽  
Sachiko Tsuda ◽  
Shinji Saito ◽  
Kyo Yamasu
Keyword(s):  
Spinal Cord ◽  
Transcription Factor ◽  
Zebrafish Embryos

scholarly journals Cellular Changes in Injured Rat Spinal Cord Following Electrical Brainstem Stimulation

2019 ◽  
Vol 9 (6) ◽  
pp. 124 ◽  
Author(s):  
Walter J. Jermakowicz ◽  
Stephanie S. Sloley ◽  
Lia Dan ◽  
Alberto Vitores ◽  
Melissa M. Carballosa-Gautam ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Cellular Proliferation ◽  
Radial Glia ◽  
Low Frequency ◽  
Cell Types ◽  
Beneficial Effects ◽  
Cell Counts ◽  
Cord Injury ◽  
Near Term ◽  
Altered Cell

Spinal cord injury (SCI) is a major cause of disability and pain, but little progress has been made in its clinical management. Low-frequency electrical stimulation (LFS) of various anti-nociceptive targets improves outcomes after SCI, including motor recovery and mechanical allodynia. However, the mechanisms of these beneficial effects are incompletely delineated and probably multiple. Our aim was to explore near-term effects of LFS in the hindbrain’s nucleus raphe magnus (NRM) on cellular proliferation in a rat SCI model. Starting 24 h after incomplete contusional SCI at C5, intermittent LFS at 8 Hz was delivered wirelessly to NRM. Controls were given inactive stimulators. At 48 h, 5-bromodeoxyuridine (BrdU) was administered and, at 72 h, spinal cords were extracted and immunostained for various immune and neuroglial progenitor markers and BrdU at the level of the lesion and proximally and distally. LFS altered cell marker counts predominantly at the dorsal injury site. BrdU cell counts were decreased. Individually and in combination with BrdU, there were reductions in CD68 (monocytes) and Sox2 (immature neural precursors) and increases in Blbp (radial glia) expression. CD68-positive cells showed increased co-staining with iNOS. No differences in the expression of GFAP (glia) and NG2 (oligodendrocytes) or in GFAP cell morphology were found. In conclusion, our work shows that LFS of NRM in subacute SCI influences the proliferation of cell types implicated in inflammation and repair, thus providing mechanistic insight into deep brain stimulation as a neuromodulatory treatment for this devastating pathology.

Subtypes of hypoxia-responsive cells differentiate into neurons in spinal cord of zebrafish embryos after hypoxic stress

2016 ◽  
Vol 108 (12) ◽  
pp. 357-377 ◽  
Author(s):  
Chih-Wei Zeng ◽  
Yasuhiro Kamei ◽  
Chih-Tien Wang ◽  
Huai-Jen Tsai
Keyword(s):  
Spinal Cord ◽  
Zebrafish Embryos ◽  
Hypoxic Stress

scholarly journals Oligodendrocytes and Radial Glia Derived From Adult Rat Spinal Cord Progenitors: Morphological and Immunocytochemical Characterization

2006 ◽  
Vol 55 (3) ◽  
pp. 209-222 ◽  
Author(s):  
Iris Kulbatski ◽  
Andrea J. Mothe ◽  
Armand Keating ◽  
Yoji Hakamata ◽  
Eiji Kobayashi ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Radial Glia ◽  
Rat Spinal Cord ◽  
Adult Rat ◽  
Adult Rat Spinal Cord
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