scholarly journals Effect of pressure and temperature on the availability of lysine in meat and bone meal as determined by slope-ratio assays with growing pigs, rats and chicks and by chemical techniques

1986 ◽  
Vol 55 (2) ◽  
pp. 441-453 ◽  
Author(s):  
E. S. Batterham ◽  
R.E. Darnell ◽  
L. S. Herbert ◽  
E. J. Major
Keyword(s):  
Late Stage ◽  
Binding Capacity ◽  
Growing Pigs ◽  
High Temperature Treatment ◽  
Slope Ratio ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Effect Of Pressure ◽  
Dye Binding ◽  
Available Lysine

1. The availability of lysine for pigs, rats and chicks was determined using samples of meat and bone meal (MBM) subjected to different pressure and temperature treatments during dry-rendering processing. The relation between slope-ratio estimates and three chemical tests for estimating ‘available’ lysine was assessed.2. The availability of lysine (proportion of total) for pigs was 0.97 in the control. Pressure (275 kPa gauge, 141°, for 30 min) in the early stage of rendering reduced availability to 0.74 and, in the late stage, to 0.46. Maintaining the final temperature at 125° for 4 h had little effect (0.84) whereas a higher temperature of 150° for 4 h reduced availability to 0.38.3. Availability estimates for rats were lower than those of the pig, ranging from 0.88 in the control to 0.21 for the high-temperature treatment (150° for 4 h). The effects for temperature were similar to those for the pig, whereas the effect of pressure was equally detrimental in both the early and late stages (0.45 and 0.43 respectively).4. For chicks, availability estimates were similar to those for the pig for the control (0.93) and the two temperature treatments (0.86 and 0.31 for the 125° and 150° treatments respectively). The chick was less susceptible to the effect of pressure applied to the MBM (0.78 and 0.63 for the early-and late-stage treatments respectively).5. Values for the indirect-and direct-1-fluoro-2, 4-dinitrobenzene-(FDNB)-‘available’-lysine assays decreased from 0.86 and 0.74 to 0.57 and 0.54 for the control and 150° for 4 h treatments respectively, indicating that approximately half the reduced availability involved reactions with the ε-amino group of lysine. There was little relation between the FDNB values and lysine availability for the treatments involving changes in pressure.6. There was little or no relation between dye-binding capacity of the meals, as assessed by the Acid Orange-12 dye-binding procedure (Hurrell et al. 1979), and lysine availability for the three species.

scholarly journals Availability of lysine in meat meal, meat and bone meal and blood meal as determined by the slope-ratio assay with growing pigs, rats and chicks and by chemical techniques

1986 ◽  
Vol 55 (2) ◽  
pp. 427-440 ◽  
Author(s):  
E. S. Batterham ◽  
R. F. Lowe ◽  
R. E. Darnell ◽  
E. J. Major
Keyword(s):  
Blood Meal ◽  
Growing Pigs ◽  
Slope Ratio ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Meat Meal ◽  
Chemical Techniques ◽  
The Individual ◽  
Blood Meals

1. The availability of lysine in four meat meals (MMs), four meat and bone meals (MBMs) and two blood meals was determined using the slope-ratio assay with growing pigs, rats and chicks and with two chemical techniques.2. The availability of lysine (proportion of total) in the eight MMs or MBMs ranged from 0.48 to 0.88 for pigs, from 0.49 to 0.88 for rats and from 0.68 to 0.88 for chicks. There was no apparent relation between the availability estimates for pigs, rats and chicks for the individual meals.3. For the two blood meals, availability estimates were 1.03 and 1.13 for pigs, 0.81 and 0.80 for rats and 1.07 and 1.02 for chicks.4. Values for the indirect and direct 1-fluoro-2, 4-dinitrobenzene-‘available’-lysineassays ranged from 0.77 to 0.88 and 0.78 to 0.93 respectively for the eight MMs and MBMs. There appeared to be no relation between these values and the pig estimates.

A nutritional evaluation of diets containing meat meal for growing pigs. 6. Amino acid and mineral-vitamin-antibiotic supplements for maize-meat meal diets

1970 ◽  
Vol 10 (46) ◽  
pp. 534 ◽  
Author(s):  
ES Batterham
Keyword(s):  
Amino Acid ◽  
Crude Protein ◽  
Growing Pigs ◽  
Meat And Bone Meal ◽  
Feed Conversion ◽  
Bone Meal ◽  
Poor Growth ◽  
Nutritional Evaluation ◽  
Meat Meal ◽  
Cent Increase

Maize-meat meal and maize-meat and bone meal diets were supplemented with dl-tryptophan, l-lysine or a mineral-vitamin-antibiotic (MVA) premix and fed to pigs during the 1s to 45 kg growth phase. Diets were offered at an estimated 145 kcal of digestible energy and 7g crude protein per kg liveweight per day. The basal maize-meat meal diet produced poor growth and feed conversion and the combined addition of all three supplements increased growth by 46 per cent, feed conversion by 21 per cent, and lean in the ham by 4.7 per cent. This effect resulted from responses to dl-tryptophan, the MVA and an interaction between dl-tryptophan and l-lysine, with l-lysine depressing gain and feed conversion in the absence of dl- tryptophan and increasing gain, feed conversion, and lean in the ham in its presence. The basal maize-meat and bone meal diet also produced poor growth and feed conversion, and the combined addition of all three supplements resulted in an 82 per cent increase in growth, a 35 per cent increase in feed conversion, and a 3.3 per cent increase in lean in the ham. Both dl-tryptophan and MYA separately increased growth and feed conversion and combined they had an even greater effect. The l-lysine increased feed conversion and lean in the ham. The l-lysine also increased growth rates on the dl-tryptophan supplemented diets, but this effect was not significant (P>0.05).

Protein quality studies. 1. A comparison of meat and bone meal and fish meal-skim milk powder as sources of supplementary protein for growing pigs

1967 ◽  
Vol 7 (29) ◽  
pp. 562 ◽  
Author(s):  
BR Wilson ◽  
JM Holder
Keyword(s):  
Dry Matter ◽  
Fish Meal ◽  
Milk Powder ◽  
Skim Milk ◽  
Skim Milk Powder ◽  
Growing Pigs ◽  
Meat And Bone Meal ◽  
Feed Conversion ◽  
Bone Meal ◽  
Dry Matter Digestibility

Pig performance was compared on wheat based diets supplemented by two levels of either meat and bone meal or fish meal plus skim milk powder. The dry matter digestibility and nitrogen retention on these diets were determined and the effect of adding zinc to diets containing meat and bone meal was examined. At the higher level, fish meal plus skim milk powder produced greater daily gains between 60-160 lb than the meat and bone meal, but feed conversion and carcase lean were not affected. At the lower level, fish meal plus skim milk powder produced leaner carcases and greater daily gains between 60-160 lb than the meat and bone meal supplement, but had no effect on feed conversion. Between 60-100 lb, feed conversion was affected by level but not by supplement, and the higher level of fish meal plus skim milk powder produced greater daily gains than all other diets. The higher levels of each supplement produced greater nitrogen retentions and leaner carcases than the lower levels. Dry matter digestibility was least on the higher level meat and bone meal. Zinc supplements had no effect on performance.

A note on the effect of sudden changes in the composition of the diet of growing pigs

1973 ◽  
Vol 16 (3) ◽  
pp. 315-317
Author(s):  
R. M. Livingstone
Keyword(s):  
Crude Protein ◽  
Fish Meal ◽  
Live Weight ◽  
Growing Pigs ◽  
The Other ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Digestible Energy ◽  
Per Se

SUMMARYTwo diets, one based on barley and white-fish meal and the other on oats, wheat and meat and bone meal, with similar concentrations of digestible energy, crude protein, lysine and methionine+cystine were used to investigate the effects of sudden changes in the composition of the diet on the performance of pigs growing from 32 kg live weight, over a period of 77 days.The diets were given separately, alternated weekly with sudden changes from one diet to the other, or as a 1:1 mixture.Changes in diet per se had insignificant effects on performance.

THE APPLICATION OF THE DYE-BINDING METHOD FOR MEASURING PROTEIN DENATURATION OF RAPESEED MEALS CAUSED BY AUTOCLAVE OR OVEN HEAT TREATMENTS FOR VARYING PERIODS OF TIME

1979 ◽  
Vol 59 (1) ◽  
pp. 189-194 ◽  
Author(s):  
Y. K. GOH ◽  
D. R. CLANDININ ◽  
A. R. ROBBLEE
Keyword(s):  
Nutritive Value ◽  
Protein Denaturation ◽  
Nitrogen Solubility ◽  
Binding Capacity ◽  
Protein Nitrogen ◽  
Acid Orange ◽  
Moisture Contents ◽  
Dye Binding ◽  
Similar Temperature ◽  
Available Lysine

Laboratory-prepared rapeseed meals (RSMs) with moisture contents ranging from 2 to 40% of the meal were heated in an autoclave or an oven at 121 °C from 15 min to 4 h. The degree of protein denaturation of the RSMs was estimated by nitrogen solubility (NS) in water and by dye-binding capacity of the protein (DBCP) with Acid Orange 12. The NS study indicated that heating RSM for 15 min to 1 h in an autoclave produced more severe denaturation than heating RSM at a similar temperature for the same periods of time in an oven. When heating in the autoclave was prolonged, the nitrogen solubility increased. The ability of the RSM protein to bind Acid Orange 12 was more severely affected by the autoclave treatment than by oven heating. In this regard, the DBCP of the RSMs was at the lowest after 2 h of heating in the autoclave and after 4 h of heating in the oven. Since the NS values for the RSMs include both protein and non-protein nitrogen while DBCP values measure mainly basic amino acids, the latter values are likely to be more valid for estimating the nutritive value of RSM. The higher correlation coefficient between DBCP and available lysine than between NS and available lysine (0.90 vs. 0.63) would seem to support this conclusion.

scholarly journals Utilization of ileal digestible amino acids by growing pigs: tryptophan

1994 ◽  
Vol 71 (3) ◽  
pp. 345-360 ◽  
Author(s):  
E. S. Batterham ◽  
L. M. Andersen ◽  
D. R. Baigent
Keyword(s):  
Cottonseed Meal ◽  
Estimation Method ◽  
Soya Bean ◽  
Growing Pigs ◽  
Growth Responses ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Ileal Digestibility ◽  
Bean Meal ◽  
Soya Bean Meal

The ileal digestibility of tryptophan for growing pigs was determined for cottonseed, meat-and-bone and soya-bean meals. Tryptophan in the food and digesta was measured by two analytical procedures (NaOH hydrolysis and colorimetric estimation (method 1) and LiOH hydrolysis and HPLC determination (method 2)). The results were respectively: cottonseed meal 0.46, 0.81; meat-and-bone meal 0.55, 0.65; soya-bean meal 0.74, 0.90. In the first experiment the values for method 1 were shown to be inapplicable to pigs. In a second experiment three tryptophan-deficient diets (005 g ileal digestible tryptophan/MJ digestible energy (DE)) were formulated using values from method 2 for cottonseed meal, meat-and-bone meal plus L-tryptophan and soya-bean meal respectively as the only sources of tryptophan in the diets. This experiment was terminated after 28 d as overall growth performance of the pigs was very low. A third experiment was conducted in a similar manner to Expt 2 except that the diets were formulated to 0.065g ileal digestible tryptophan/MJ DE and growth responses and tryptophan retention were assessed over the 20–45 kg growth phase. Growth rates (g.d) of the pigs given the three diets were significantly different (P < 0.01): cottonseed meal 393, meat-and-bone meal plus L-tryptophan 531, soya-bean meal 437 (SED 39.0). Tryptophan retention (as a proportion of ileal digestible tryptophan intake) was significantly different (P < 0.05): cottonseed meal 0.51, meat-and-bone meal plus L-tryptophan 0.49, soya-bean meal 0.41. These results indicate (1) that the colorimetric technique for assessing tryptophan was inapplicable and (2) that ileal digestible values for tryptophan were not suitable for formulating diets containing heat-processed proteins, possibly due to absorption of some of the tryptophan in a form that was non-utilizable, and/or to underestimation of total tryptophan in the protein concentrates.

scholarly journals EQUATIONS TO PREDICT THE METABOLIZABLE ENERGY OF MEAT AND BONE MEAL FOR GROWING PIGS

2015 ◽  
Vol 39 (6) ◽  
pp. 565-573 ◽  
Author(s):  
Ricardo Araújo Castilho ◽  
Paulo Cesar Pozza ◽  
Newton Tavares Escocard de Oliveira ◽  
Cleiton Pagliari Sangali ◽  
Carolina Natali Langer ◽  
...  
Keyword(s):  
Block Design ◽  
Basal Diet ◽  
Growing Pigs ◽  
Metabolizable Energy ◽  
Good Prediction ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Randomized Block Design ◽  
Dependent Parameter ◽  
Energetic Composition

ABSTRACT The prediction of metabolizable energy (ME) of meat and bone meal (MBM) for pigs is an interesting tool, however, used models to predict these values must be validated in order to garantee higher precision. The aim of this study was to determine the chemical and energetic composition of different types of MBM for pigs and to adjust and validate models to better predict the ME based on the chemical composition. Thirty-two barrows, averaging an initial weight of 26.75 ± 1.45 kg, were individually allotted in a randomized block design with eight treatments and four replicates. The treatments consisted of seven types of MBM that replaced 20% of the basal diet. A stepwise procedure was the statistical procedure used to adjust the prediction equations and the ME was the dependent parameter. The validation of the adjusted models was performed using an independent databank of chemical and energetic composition of theBrazilian and international MBM. The metabolizable energy of different meat and bone meals ranged from 1645 to 2645 kcal kg-1. The equations that provide a good prediction of metabolizable energy of meat and bone meal for swine in Brazil are EM1 = -4233.58 + 0.4134GE + 72CP + 89.62ash - 159.06Ca; EM2 = 2087.49 + 0.3446GE + 31.82ash - 189.18Ca; EM3 = 2140.13 + 0.3845GE - 112.33Ca; EM4 = -346.58 + 0.656GE; EM5 = 3221.27 + 178.96fat - 76.55ash; and EM6 = 5356.45 - 84.75ash.

scholarly journals Availability of lysine in protein concentrates as determined by the slope-ratio assay with chicks and comparisons with rat, pig and chemical assays

1981 ◽  
Vol 46 (3) ◽  
pp. 513-519 ◽  
Author(s):  
E. J. Major ◽  
E. S. Batterham
Keyword(s):  
Cottonseed Meal ◽  
Fish Meal ◽  
Basal Diet ◽  
Slope Ratio ◽  
Meat And Bone Meal ◽  
Sunflower Meal ◽  
Protein Concentrates ◽  
Available Lysine ◽  
Bean Meal ◽  
Soya Bean Meal

1. A slope-ratio assay was developed to determine the availability of lysine in protein concentrates for chicks, Two protein concentrates were assessed per assay, using three levels of incorporation into the basal diet.2. Availability of lysine, expressed as a proportion of total lysine in five protein concentrates was: cottonseed meal 0·83, fish meal 1·00, meat-and-bone meal 0·86, soya-bean meal 0·93, sunflower meal 1·01.3. The five protein concentrates had previously been assayed for available lysine with slope-ratio assays for pies and rats (Batterham et al. 1979; Batterharn et al. 1981), There was little relationship between the results for chicks and those for pigs and rats. For pigs, availability estimates ranged from 0·43 for cottonseed meal to 0·89 for fish meal. For rats, availability estimates ranged from 0·49 for sunflower meal to 1·04 for fish meal.4. The results for chicks were in closer agreement with values obtained using the Silcock available-lysine assay (Roach et al. 1967) and the direct 1-fluoro-2,4-dinitrobenzene procedure (Carpenter, 1960). By contrast, there was litlle relationship between the chemical tests and results for pigs or rats.

scholarly journals Nutritional value of lupin (Lupinus albus)-seed meal for growing pigs: availability of lysine, effect of autoclaving and net energy content

1986 ◽  
Vol 56 (3) ◽  
pp. 645-659 ◽  
Author(s):  
E. S. Batterham ◽  
L. M. Andersen ◽  
R. F. Lowe ◽  
R. E. Darnell
Keyword(s):  
Lupinus Albus ◽  
Soya Bean ◽  
Growing Pigs ◽  
Seed Meal ◽  
Net Energy ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Lupin Seed ◽  
Bean Meal ◽  
Soya Bean Meal

1.Two experiments were conducted to assess the nutritional value of lupin (Lupinus albus)-seed meal for growing pigs. In the first, the availability of lysine was assessed using slope-ratio analysis. In the second, the effects of autoclaving lupin seeds and formulating the diets on the basis of estimated digestible or net energy were assessed.2. In the first experiment, the availability of lysine in three samples of lupin-seed meal was compared with that in meat-and-bone meal and soya-bean meal. Availability of lysine in the five protein concentrates, using food conversion efficiency on a carcass basis as the criterion of response, was (proportion of total): lupin-seed meal no. 1 0.44, no. 2 0.57, no. 3 0.53, meat-and-bone meal 0.42, soya-bean meal 0.80.3. Availability estimates, based on protein deposited:food intake, were: lupin-seed meal no. 1 0.82, no. 2 0.73, no. 3 0.70, meat-and-bone meal 0.27, soya-bean meal 0.77. These estimates had higher standard deviations than those based on carcass response.4. Regressing the measures of response v. lysine intake resulted in estimates of availability similar to, or higher than, the slope-ratio analysis but was associated with greater statistical invalidity and higher standard deviations.5. The proportion of energy retained in the carcasses was unaffected by the inclusion levels of lysine or soya-bean meal. Energy retention was depressed (P < 0.05) with the three lupin-seed meals and the meat-and-bone meal.6. In the second experiment, the response of pigs given a diet containing lupin-seed meal was inferior, on a carcass basis (P < 0.05), to that of pigs given a diet containing soya-bean meal formulated to similar total lysine and digestible energy contents.7. The addition of soya-bean oil to the diet containing lupin-seed meal, to equalize the estimated net energy of the diet to that of the diet containing soya-bean meal, depressed protein deposition (P < 0.05) and increased fat deposition (P < 0.05), indicating that energy was not limiting the growth of pigs given the lupin-seed-meal diet.8. Autoclaving the lupin-seed at 121°for 5 min had no effect on the growth of pigs, indicating that the low availability of lysine was not due to the presence of heat-labile anti-nutritional factors.

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