Development and Evaluation of a Real-Time FRET Probe Based Multiplex PCR Assay for the Detection of Prohibited Meat and Bone Meal in Cattle Feed and Feed Ingredients

2006 ◽  
Vol 3 (4) ◽  
pp. 337-346 ◽  
Author(s):  
Gabriel J. Rensen ◽  
Wayne L. Smith ◽  
Carmela V. Jaravata ◽  
Bennie Osburn ◽  
James S. Cullor
Keyword(s):  
Multiplex Pcr ◽  
Real Time ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Pcr Assay ◽  
Feed Ingredients ◽  
Cattle Feed ◽  
Multiplex Pcr Assay

scholarly journals Multiplex PCR assay for animal species identification in meat bone meal

2019 ◽  
Vol 387 ◽  
pp. 012018
Author(s):  
M Cahyadi ◽  
T Wibowo ◽  
Y P Nugraheni ◽  
A Fadhila ◽  
A Pramono ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Species Identification ◽  
Animal Species ◽  
Bone Meal ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Meat Bone Meal

Real-Time Polymerase Chain Reaction Detection of Bovine DNA in Meat and Bone Meal Samples

2002 ◽  
Vol 65 (7) ◽  
pp. 1158-1165 ◽  
Author(s):  
S. LAHIFF ◽  
M. GLENNON ◽  
J. LYNG ◽  
T. SMITH ◽  
N. SHILTON ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Chain Reaction ◽  
Pcr Assay ◽  
The Real ◽  
Pcr Product ◽  
Pcr Products ◽  
Polymerase Chain

We describe a real-time polymerase chain reaction (PCR) assay for the detection of bovine DNA extracted from meat and bone meal (MBM) samples. PCR primers were used to amplify a 271-bp region of the mitochondrial ATPase 8–ATPase 6 gene, and a fluorogenic probe (BOV1) labeled with a 5′ FAM reporter and a 3′ TAMRA quencher was designed to specifically detect bovine PCR product. The specificity of the BOV1 probe for the detection of the bovine PCR product was confirmed by Southern blot hybridization analysis of the probe with PCR products generated from ovine, porcine, and bovine genomic DNA extracted from blood and with PCR products generated from genomic DNA extracted from single-species laboratory scale rendered MBM samples. The specificity of the BOV1 probe was also evaluated in real-time PCR reactions including these genomic targets. Both methods demonstrated that the BOV1 probe was specific for the detection of bovine PCR product. The BOV1 probe had a detection limit of 0.0001% bovine material by Southern blot DNA probe hybridization analysis and a detection limit of 0.001% bovine material in the real-time PCR assay. Application of the real-time PCR assay to six industrial samples that had previously tested positive for the presence of bovine material with a conventional PCR assay yielded positive results with the real-time PCR assay for four samples.

scholarly journals Real-Time Multiplex PCR Assay and Melting Curve Analysis for Identifying Diarrheagenic Escherichia coli

2013 ◽  
Vol 51 (3) ◽  
pp. 1031-1033 ◽  
Author(s):  
Tamara B. Souza ◽  
Diego M. Lozer ◽  
Sônia M. S. Kitagawa ◽  
Liliana C. Spano ◽  
Neusa P. Silva ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multiplex Pcr ◽  
Real Time ◽  
Melting Curve ◽  
Curve Analysis ◽  
Melting Curve Analysis ◽  
Pcr Assay ◽  
Diarrheagenic Escherichia Coli ◽  
Multiplex Pcr Assay

Development of a real-time multiplex PCR assay for detection of viral pathogens of penaeid shrimp

2008 ◽  
Vol 153 (12) ◽  
pp. 2245-2251 ◽  
Author(s):  
Zhixun Xie ◽  
Liji Xie ◽  
Yaoshan Pang ◽  
Zhaofa Lu ◽  
Zhiqin Xie ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Real Time ◽  
Penaeid Shrimp ◽  
Pcr Assay ◽  
Viral Pathogens ◽  
Multiplex Pcr Assay
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